20200506_ METABAT__266_1-contigs.fa

Organism: uncultured Pseudomonadales bacterium
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; environmental samples

Attributes

collection date	2020-05-06
broad-scale environmental context	marine biome [ENVO:00000447]
local-scale environmental context	microbial feature [ENVO:00002150]
environmental medium	coastal sea water [ENVO:00002150]
geographic location	North Sea
investigation type	metagenome-assembled genome
isolation source	marine water
project name	2020 Helgoland Spring Bloom Metagenomes
sample name	PACB-20200506-m63
ENA-CHECKLIST	ERC000047
ENA-FIRST-PUBLIC	2022-09-23
ENA-LAST-UPDATE	2022-09-23
External Id	SAMEA110069425
INSDC center alias	Max Planck Institute for Marine Microbiology
INSDC center name	Max Planck Institute for Marine Microbiology
INSDC first public	2022-09-23T08:24:31Z
INSDC last update	2022-09-23T08:24:31Z
INSDC status	public
Submitter Id	PACB-20200506-m63
assembly quality	Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S, and 5S rRNA genes and at least 18 tRNAs
assembly software	Flye v2.8.3
binning parameters	anvi'o 6.2, default parameters
binning software	Metabat2 v 2.12.1 and manual refinement with anvi’o v6.2
completeness score	96.2
completeness software	CheckM v1.1.3
contamination score	1.3
geographic location (latitude)	54.19
geographic location (longitude)	7.9
metagenomic source	marine metagenome
sample derived from	ERS12037258
sequencing method	Pacific Biosciences Sequel II
taxonomic identity marker	multi-marker approach (GTDB-tk v1.7, GTDB v202)

Description: seawater was pumped into a 500 L carboy, brought ashore, subsequently filtered through 10 μm - and 3 μm - pore-size polycarbonate filters and finally filtered onto 0.2 μm pore-size polycarbonate filter, frozen at -80°C. This sample refers to the 0.2 - 3 μm fraction

BioSample