20052020_METABAT_321_1

Organism: uncultured Sphingomonadales bacterium
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; Sphingomonadales; environmental samples

Attributes

collection date	2020-05-20
broad-scale environmental context	marine biome [ENVO:00000447]
local-scale environmental context	microbial feature [ENVO:00002150]
environmental medium	coastal sea water [ENVO:00002150]
geographic location	North Sea
investigation type	metagenome-assembled genome
isolation source	marine water
project name	2020 Helgoland Spring Bloom Metagenomes
sample name	AK_MB321_1
ENA-CHECKLIST	ERC000047
ENA-FIRST-PUBLIC	2022-09-23
ENA-LAST-UPDATE	2022-09-23
External Id	SAMEA110056013
INSDC center alias	Max Planck Institute for Marine Microbiology
INSDC center name	Max Planck Institute for Marine Microbiology
INSDC first public	2022-09-23T08:24:25Z
INSDC last update	2022-09-23T08:24:25Z
INSDC status	public
Submitter Id	AK_MB321_1
assembly quality	Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly software	Flye v2.8.3
binning parameters	anvi'o 7, default parameters
binning software	Metabat2 and manual refinement with anvi’o v7
completeness score	86.02
completeness software	CheckM v1.0.18
contamination score	0.87
geographic location (latitude)	54.19
geographic location (longitude)	7.9
metagenomic source	marine metagenome
sample derived from	ERS12037267
sequencing method	Pacific Biosciences Sequel II
taxonomic identity marker	multi-marker approach (GTDB-tk v1.3.0, GTDB v202)

Description: seawater was pumped into a 500 L carboy, brought ashore, subsequently filtered through 10 μm - and 3 μm - pore-size polycarbonate filters and finally filtered onto 0.2 μm pore-size polycarbonate filter, frozen at -80°C. This sample refers to the 0.2 - 3 μm fraction

BioSample