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SARS-CoV-2: wastewater surveillance sample from wastewater metagenome

Identifiers
BioSample: SAMN45913980; Sample name: 770309001; SRA: SRS23600061
Organism
wastewater metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes
Package
SARS-CoV-2: wastewater surveillance; version 1.0
Attributes
collection date2024-12-03
geographic locationUSA: Wisconsin
isolation sourcewastewater
wastewater population<100000
wastewater sample duration24
wastewater sample matrixraw wastewater
wastewater sample typecomposite
purpose of wastewater samplingpublic health surveillance community-level
purpose of wastewater sequencingIdentification of the diversity of mutations across the SARS-CoV-2 metagenome
sequenced byWisconsin State Laboratory of Hygiene
wastewater endogenous control 1pepper mild mottle virus
wastewater endogenous control 1 concentration104240000
wastewater endogenous control 1 protocolIDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
wastewater endogenous control 1 unitscopies/L
wastewater flow8645880.5512
wastewater population sourcepopulation of jurisdiction encompassing the wastewater service area
wastewater primary sludge retention timenot applicable
wastewater processing protocolInternal control spiked in samples at receipt. Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
wastewater sample sitewastewater treatment plant
wastewater surveillance jurisdictionWI
wastewater surveillance system sample ID770309001
project nameWisconsin SARS-CoV-2 Wastewater Monitoring Network
wastewater surveillance target 1SARS-CoV-2
wastewater surveillance target 1 geneN gene (N1)
wastewater surveillance target 1 known presentyes
wastewater surveillance target 1 protocolIDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
wastewater surveillance target 1 concentration645440
wastewater surveillance target 1 conc unitcopies/L
wastewater surveillance target 2SARS-CoV-2
wastewater surveillance target 2 geneN gene (N2)
wastewater surveillance target 2 known presentyes
wastewater surveillance target 2 protocolIDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
wastewater surveillance target 2 conc663040
wastewater surveillance target 2 conc unitcopies/L
concentration_methodceres nanotrap
extraction_methodpromega ht tna kit
extraction_controlbovine coronavirus
BioProject
PRJNA889839 wastewater metagenome
Retrieve all samples from this project

Submission
Wisconsin State Laboratory of Hygiene, Adelaide Roguet; 2024-12-19
Accession:
SAMN45913980
ID:
45913980

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