SARS-CoV-2: wastewater surveillance sample from wa... - BioSample

SARS-CoV-2: wastewater surveillance sample from wastewater metagenome

Identifiers: BioSample: SAMN45913979; Sample name: 770083001; SRA: SRS23600060

Organism: wastewater metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes

Package: SARS-CoV-2: wastewater surveillance; version 1.0

Attributes

collection date	2024-12-02
geographic location	USA: Wisconsin
isolation source	wastewater
wastewater population	<100000
wastewater sample duration	24
wastewater sample matrix	raw wastewater
wastewater sample type	composite
purpose of wastewater sampling	public health surveillance community-level
purpose of wastewater sequencing	Identification of the diversity of mutations across the SARS-CoV-2 metagenome
sequenced by	Wisconsin State Laboratory of Hygiene
wastewater endogenous control 1	pepper mild mottle virus
wastewater endogenous control 1 concentration	141600000
wastewater endogenous control 1 protocol	IDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
wastewater endogenous control 1 units	copies/L
wastewater flow	7267990.656
wastewater pH	7.38
wastewater population source	population of jurisdiction encompassing the wastewater service area
wastewater primary sludge retention time	not applicable
wastewater processing protocol	Internal control spiked in samples at receipt. Samples were concentrated using Nanotrap Microbiome A beads combined with the enhancement reagent 2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
wastewater sample site	wastewater treatment plant
wastewater surveillance jurisdiction	WI
wastewater surveillance system sample ID	770083001
project name	Wisconsin SARS-CoV-2 Wastewater Monitoring Network
wastewater surveillance target 1	SARS-CoV-2
wastewater surveillance target 1 gene	N gene (N1)
wastewater surveillance target 1 known present	yes
wastewater surveillance target 1 protocol	IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
wastewater surveillance target 1 concentration	285760
wastewater surveillance target 1 conc unit	copies/L
wastewater surveillance target 2	SARS-CoV-2
wastewater surveillance target 2 gene	N gene (N2)
wastewater surveillance target 2 known present	yes
wastewater surveillance target 2 protocol	IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
wastewater surveillance target 2 conc	357440
wastewater surveillance target 2 conc unit	copies/L
concentration_method	ceres nanotrap
extraction_method	promega ht tna kit
extraction_control	bovine coronavirus

Submission: Wisconsin State Laboratory of Hygiene, Adelaide Roguet; 2024-12-19

Accession:: SAMN45913979
ID:: 45913979

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SARS-CoV-2: wastewater surveillance sample from wastewater metagenome

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