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Organizing biological data
Thermus thermophilus
HB27-WT-RNAseq
I-B crRNA sequencing
Interference Requirements of Type III CRISPR-Cas Systems from Thermus thermophilus
mutantTth202204
Combination of clean mutations in the Primpol gene and catalytic Primpol mutants with clean deletions of the addAB operon
DNA repair pathways are important in the biology of the bacterium Thermus thermophilus.
The main objective is testing how important the RecFOR pathway genes RecO and RecR are in Thermus thermophilus biology, toghether with the downstream resolvase RuvB, whose product is part of the RuvABC complex.
Verify the transposition events in genomic DNA from 10 different strains of Thermus thermophilus.
Genomic DNA from 10 different strains of Thermus thermophilus were selected on kanamycin after transformation of a Thermus thermophilus Primpol gene deletion mutant with Thermus thermophilus genomic DNA that has been subject to a transposition reaction with a Tn5 transposase and a transposable fragment that contains a kanamycin resistance cassette in order to verify the transposition events.
Whole genome sequencing of five Thermus thermophilus HB27 strains used to study the physiological role of Ttago and TthPrimpol in DNA interference.
This project aims to sequence, in a whole-genome scale, five derivates of the thermophilic bacteria Thermus thermophilus HB27 in order to detect SNPs and compare the differences between mutants and its parental strains, quantify plasmid copy number of each strain and study the coverage of lectures along the genome.
Thermus thermophilus HB27
Thermus thermophilus HB27 Genome sequencing
Thermus thermophilus HB27 Raw sequence reads
The Thermus thermophilus DEAD-box protein Hera is a general RNA chaperone and plays a key role in tRNA metabolism
Effects of Argonaute on gene expression in Thermus thermophilus
To investigate if TtAgo also has the potential to control RNA levels, we analyzed RNA-seq data derived from cultures of four T. thermophilus strain HB27 variants: wild type, TtAgo knockout (Δago), and either strain transformed with a plasmid. Additionally we determined the effect of TtAgo on expression of plasmid-encoded RNA and plasmid DNA levels.
Thermus thermophilus HB27 WT vs HB27Δago RNAseq
Refseq Prokaryotic Genome Annotation Project
Thermophilic bacterium that produces thermostable enzymes of use in industry
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