BioProject

Recent advances in genomics have addressed the challenge that divergent haplotypes pose to the reconstruction of haploid genomes. More...

Recent advances in genomics have addressed the challenge that divergent haplotypes pose to the reconstruction of haploid genomes. However for many organisms, the sequencing of either field-caught individuals or a pool of heterogeneous individuals is still the only practical option. Here we present methodological approaches to achieve three outcomes from pooled long read sequencing, the generation of a contiguous haploid reference sequence, the sequences of heterozygous haplotypes and reconstructed genomic sequences of individuals related to the pooled material. PacBio long read sequencing, Dovetail Hi-C scaffolding and linkage map integration yielded a haploid chromosome-level assembly for the diamondback moth (Plutella xylostella), a global pest of Brassica crops, from a pool of related individuals. Less...