See
Genome Information for Mus musculus
We hypothesize that gene expression in the CBs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) genes related to CB function are expressed less in the A/J mice compared to DBA/2J mice; (2) gene expression levels of morphogenic and trophic factors of the CB maybe significantly lower in the A/J mice than the DBA/2J mice. In order to test these hypotheses, we utilized microarray analysis to examine transcriptional differences between CBs of both strains of mice.
Keywords: comparative expression profiling
Overall design: . This study utilizes microarray analysis to test these hypotheses. Three sets of CBs were harvested from both strains. RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05.
| Accession | PRJNA96321; GEO: GSE5313 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mus musculus[Taxonomy ID: 10090] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus |
| Publications | Balbir A et al., "A search for genes that may confer divergent morphology and function in the carotid body between two strains of mice.", Am J Physiol Lung Cell Mol Physiol, 2007 Mar;292(3):L704-15 |
| Submission | Registration date: 2-Dec-2006
Division of Physiology, Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
|---|
| Publications |
| PubMed | 1 |
| Other datasets |
| GEO DataSets | 2 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 270606 |
| Data volume, Processed Mbytes | 8 |