The municipal solid waste (MSW) is a serious and progressively growing environmental challenge in India. Pirana landfill dumping site (Ahmedabad, Gujarat) is the seventh largest open landfill of India where low and high density polyethylene (L/H-DPE) waste constitute 88 % of total plastic waste. During the recent COVID-19 pandemic, LDPE waste was found to be a major component of the MSW in terms of weight and volume. The conventional processing of these plastic waste poses major health and environmental risks. Therefore, microorganism mediated bioremediation has been observed to be an eco-friendly approach. In this study, six bacterial cultures (PSM1 to PSM6) were isolated from soil collected from Pirana landfill dump site, Ahmedabad, Gujarat, India. Genomic DNA from all the isolates was extracted and subjected to whole genome sequencing. Whole genome sequence was assembled and analysed for their identification which revealed the genera Rossellomorea, Klebsiella, Alcaligenes, and Bacillus. Furthermore, the annotation of draft genomes revealed the presence of plastic degrading enzymes such as monooxygenases, dioxygenases, hydroxylases, dehydrogenases, hydratases, lyases, esterases, transferases and peroxidases. The results provide the primary basis for further investigation on LDPE degradation mechanisms as well as bacterial sources for the potential plastic degrading enzymes.
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