BioProject

Assessing the dynamics of chromatin and transcription factor (TF) DNA binding in plants remains a significant challenge, primarily due to the lack of efficient tools for capturing these features on a larger scale. More...

Assessing the dynamics of chromatin and transcription factor (TF) DNA binding in plants remains a significant challenge, primarily due to the lack of efficient tools for capturing these features on a larger scale. Here, we present PHILO (Plant HIgh-throughput LOw input) ChIP-seq, a high-throughput ChIP-seq platform that enables the cost-effective and extensive capture of TF DNA binding and the genome-wide distribution of histone modifications. Through the application of H3K9ac PHILO ChIP-seq to eight jasmonic acid pathway mutants, involving the parallel processing of over 100 samples, we not only substantially corroborate the previously described interplay among chromatin regulators at the target genes of the JA master TF MYC2 but also unveil previously unknown histone acetylation patterns in the regulatory regions of MYC2 target genes. Overall, our PHILO ChIP-seq platform proves to be a highly effective approach for simultaneously examining chromatin on an unprecedented scale opening the door to conducting large-scale epigenome studies in plants. Overall design: ChIP-seq and PHILO ChIP-seq for histone modifications H3K9c, H3K4me3, H3K27me3, H3K36me3, transcription factor MYC2 and Pol II in Arabidopsis wildtype Col-0 seedlings and various jasmonic acid pathway mutants. Less...