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Conserved domains on  [gi|1958642903|ref|XP_038958618|]
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fatty acid desaturase 3 isoform X1 [Rattus norvegicus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PLN03199 super family cl31982
delta6-acyl-lipid desaturase-like protein; Provisional
 122-340 7.60e-29

delta6-acyl-lipid desaturase-like protein; Provisional


The actual alignment was detected with superfamily member PLN03199:

Pssm-ID: 178740 [Multi-domain]  Cd Length: 485  Bit Score: 116.29  E-value: 7.60e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 122 FRWEQIRQHDLPGDKWLVIERRVYDISRWAQrHPGGSrLIGHHSAEDATDAFHAFHQDLHfvRKFLKPLLIGELAPEEPS 201
Cdd:PLN03199   26 ISWQEVKKHASPDDAWIIHQNKVYDVSNWHD-HPGGA-VIFTHAGDDMTDIFAAFHAPGS--QALMKKFYIGDLIPESTE 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 202 QDGAQNAQLIEDFRALRQAAEDMKLFEADTTFFALLLGHILAMELLAWLLVYLLGPGWVSsVLAALILAISQAQCWCLQH 281
Cdd:PLN03199  102 HKDPQQIAFEKGYRDLRAKLIMMGMFKSNKMFYAYKCLFNMAIWAAACALVFYSDRFAMH-IASALLLGLFFQQCGWLAH 180

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958642903 282 DLGHASIFPKSRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHK-------DPDVTVAPVF 340
Cdd:PLN03199  181 DFLHHQVFKKRKHGDLGGIFWGDLMQGFSMQWWKNKHNGHHAVPNLHCSsadaqdgDPDIDTMPLL 246
 
Name Accession Description Interval E-value
PLN03199 PLN03199
delta6-acyl-lipid desaturase-like protein; Provisional
 122-340 7.60e-29

delta6-acyl-lipid desaturase-like protein; Provisional


Pssm-ID: 178740 [Multi-domain]  Cd Length: 485  Bit Score: 116.29  E-value: 7.60e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 122 FRWEQIRQHDLPGDKWLVIERRVYDISRWAQrHPGGSrLIGHHSAEDATDAFHAFHQDLHfvRKFLKPLLIGELAPEEPS 201
Cdd:PLN03199   26 ISWQEVKKHASPDDAWIIHQNKVYDVSNWHD-HPGGA-VIFTHAGDDMTDIFAAFHAPGS--QALMKKFYIGDLIPESTE 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 202 QDGAQNAQLIEDFRALRQAAEDMKLFEADTTFFALLLGHILAMELLAWLLVYLLGPGWVSsVLAALILAISQAQCWCLQH 281
Cdd:PLN03199  102 HKDPQQIAFEKGYRDLRAKLIMMGMFKSNKMFYAYKCLFNMAIWAAACALVFYSDRFAMH-IASALLLGLFFQQCGWLAH 180

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958642903 282 DLGHASIFPKSRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHK-------DPDVTVAPVF 340
Cdd:PLN03199  181 DFLHHQVFKKRKHGDLGGIFWGDLMQGFSMQWWKNKHNGHHAVPNLHCSsadaqdgDPDIDTMPLL 246
Delta6-FADS-like cd03506
The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like CD includes the integral-membrane enzymes: ...
 263-344 1.86e-24

The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like CD includes the integral-membrane enzymes: delta-4, delta-5, delta-6, delta-8, delta-8-sphingolipid, and delta-11 desaturases found in vertebrates, higher plants, fungi, and bacteria. These desaturases are required for the synthesis of highly unsaturated fatty acids (HUFAs), which are mainly esterified into phospholipids and contribute to maintaining membrane fluidity. While HUFAs may be required for cold tolerance in bacteria, plants and fish, the primary role of HUFAs in mammals is cell signaling. These enzymes are described as front-end desaturases because they introduce a double bond between the pre-exiting double bond and the carboxyl (front) end of the fatty acid. Various substrates are involved, with both acyl-coenzyme A (CoA) and acyl-lipid desaturases present in this CD. Acyl-lipid desaturases are localized in the membranes of cyanobacterial thylakoid, plant endoplasmic reticulum (ER), and plastid; and acyl-CoA desaturases are present in ER membrane. ER-bound plant acyl-lipid desaturases and acyl-CoA desaturases require cytochrome b5 as an electron donor. Most of the eukaryotic desaturase domains have an adjacent N-terminal cytochrome b5-like domain. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXH, HXX(X)HH, and Q/HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homolog, stearoyl CoA desaturase.


Pssm-ID: 239583 [Multi-domain]  Cd Length: 204  Bit Score: 98.87  E-value: 1.86e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 263 VLAALILAISQAQCWCLQHDLGHASIFPKSRWNHVAQQFVMGqLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPVFLL 342
Cdd:cd03506     1 LLLAILLGLFWAQGGFLAHDAGHGQVFKNRWLNKLLGLTVGN-LLGASAGWWKNKHNVHHAYTNILGHDPDIDTLPLLAR 79


                  ..
gi 1958642903 343 GE 344
Cdd:cd03506    80 SE 81
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 123-196 4.07e-24

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 93.84  E-value: 4.07e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958642903 123 RWEQIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLHFVRKFLKPLLIGELA 196
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEAIGHSEDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 125-196 1.03e-14

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 68.91  E-value: 1.03e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1958642903 125 EQIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLHFVRKFLKPLLIGELA 196
Cdd:COG5274    21 AEVATHNTLSDCWMAIDGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFNTKHPHSPKAERLLESYRIGRLA 92
 
Name Accession Description Interval E-value
PLN03199 PLN03199
delta6-acyl-lipid desaturase-like protein; Provisional
 122-340 7.60e-29

delta6-acyl-lipid desaturase-like protein; Provisional


Pssm-ID: 178740 [Multi-domain]  Cd Length: 485  Bit Score: 116.29  E-value: 7.60e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 122 FRWEQIRQHDLPGDKWLVIERRVYDISRWAQrHPGGSrLIGHHSAEDATDAFHAFHQDLHfvRKFLKPLLIGELAPEEPS 201
Cdd:PLN03199   26 ISWQEVKKHASPDDAWIIHQNKVYDVSNWHD-HPGGA-VIFTHAGDDMTDIFAAFHAPGS--QALMKKFYIGDLIPESTE 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 202 QDGAQNAQLIEDFRALRQAAEDMKLFEADTTFFALLLGHILAMELLAWLLVYLLGPGWVSsVLAALILAISQAQCWCLQH 281
Cdd:PLN03199  102 HKDPQQIAFEKGYRDLRAKLIMMGMFKSNKMFYAYKCLFNMAIWAAACALVFYSDRFAMH-IASALLLGLFFQQCGWLAH 180

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958642903 282 DLGHASIFPKSRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHK-------DPDVTVAPVF 340
Cdd:PLN03199  181 DFLHHQVFKKRKHGDLGGIFWGDLMQGFSMQWWKNKHNGHHAVPNLHCSsadaqdgDPDIDTMPLL 246
PLN03198 PLN03198
delta6-acyl-lipid desaturase; Provisional
 126-326 1.12e-25

delta6-acyl-lipid desaturase; Provisional


Pssm-ID: 178739 [Multi-domain]  Cd Length: 526  Bit Score: 107.47  E-value: 1.12e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 126 QIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSrLIGHHSAEDATDAFHAFHQDLHFvrKFLKPLLIGELAPEEPSqdga 205
Cdd:PLN03198  110 EVAAHNKPNDCWIVIKNKVYDVSDFAAEHPGGS-VISTYFGRDGTDAFSSFHAASTW--KILQDFYIGDVDNVEPT---- 182

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 206 qnAQLIEDFRALRQAAEDMKLFEADTTFFALLLGHILAMELLAWLLVYLLGPGWvsSVLA-ALILAISQAQCWCLQHDLG 284
Cdd:PLN03198  183 --PELLKDFRDLRALFLREQLFKSSKLYYVFKLLTNIAIFAASIAIICCSKSIS--AVLAsACMMALCFQQCGWLSHDFL 258

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1958642903 285 HASIFpKSRW-NHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPN 326
Cdd:PLN03198  259 HNQVF-ETRWlNEVVGYLIGNAVLGFSTGWWKEKHNLHHAAPN 300
Delta6-FADS-like cd03506
The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like CD includes the integral-membrane enzymes: ...
 263-344 1.86e-24

The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like CD includes the integral-membrane enzymes: delta-4, delta-5, delta-6, delta-8, delta-8-sphingolipid, and delta-11 desaturases found in vertebrates, higher plants, fungi, and bacteria. These desaturases are required for the synthesis of highly unsaturated fatty acids (HUFAs), which are mainly esterified into phospholipids and contribute to maintaining membrane fluidity. While HUFAs may be required for cold tolerance in bacteria, plants and fish, the primary role of HUFAs in mammals is cell signaling. These enzymes are described as front-end desaturases because they introduce a double bond between the pre-exiting double bond and the carboxyl (front) end of the fatty acid. Various substrates are involved, with both acyl-coenzyme A (CoA) and acyl-lipid desaturases present in this CD. Acyl-lipid desaturases are localized in the membranes of cyanobacterial thylakoid, plant endoplasmic reticulum (ER), and plastid; and acyl-CoA desaturases are present in ER membrane. ER-bound plant acyl-lipid desaturases and acyl-CoA desaturases require cytochrome b5 as an electron donor. Most of the eukaryotic desaturase domains have an adjacent N-terminal cytochrome b5-like domain. This domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain the residues: HXXXH, HXX(X)HH, and Q/HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homolog, stearoyl CoA desaturase.


Pssm-ID: 239583 [Multi-domain]  Cd Length: 204  Bit Score: 98.87  E-value: 1.86e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 263 VLAALILAISQAQCWCLQHDLGHASIFPKSRWNHVAQQFVMGqLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPVFLL 342
Cdd:cd03506     1 LLLAILLGLFWAQGGFLAHDAGHGQVFKNRWLNKLLGLTVGN-LLGASAGWWKNKHNVHHAYTNILGHDPDIDTLPLLAR 79


                  ..
gi 1958642903 343 GE 344
Cdd:cd03506    80 SE 81
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 123-196 4.07e-24

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 93.84  E-value: 4.07e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958642903 123 RWEQIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLHFVRKFLKPLLIGELA 196
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAINGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEAIGHSEDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 125-196 1.03e-14

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 68.91  E-value: 1.03e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1958642903 125 EQIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLHFVRKFLKPLLIGELA 196
Cdd:COG5274    21 AEVATHNTLSDCWMAIDGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFNTKHPHSPKAERLLESYRIGRLA 92
DesA COG3239
Fatty acid desaturase [Lipid transport and metabolism];
212-335 3.40e-13

Fatty acid desaturase [Lipid transport and metabolism];


Pssm-ID: 442471 [Multi-domain]  Cd Length: 319  Bit Score: 69.37  E-value: 3.40e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 212 EDFRALRQAAEDmKLFEADTTFFALLLGHILAMELLAWLLvyllGPGWVSsVLAALILAISQAQCWCLQHDLGHASIFPK 291
Cdd:COG3239    13 AELRALRARLRA-LLGRRDWRYLLKLALTLALLAALWLLL----SWSWLA-LLAALLLGLALAGLFSLGHDAGHGSLFRS 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1958642903 292 SRWNHVAqQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVT 335
Cdd:COG3239    87 RWLNDLL-GRLLGLPLGTPYDAWRRSHNRHHAYTNDPGKDPDIG 129
Membrane-FADS-like cd01060
The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane ...
 263-336 3.81e-13

The membrane fatty acid desaturase (Membrane_FADS)-like CD includes membrane FADSs, alkane hydroxylases, beta carotene ketolases (CrtW-like), hydroxylases (CrtR-like), and other related proteins. They are present in all groups of organisms with the exception of archaea. Membrane FADSs are non-heme, iron-containing, oxygen-dependent enzymes involved in regioselective introduction of double bonds in fatty acyl aliphatic chains. They play an important role in the maintenance of the proper structure and functioning of biological membranes. Alkane hydroxylases are bacterial, integral-membrane di-iron enzymes that share a requirement for iron and oxygen for activity similar to that of membrane FADSs, and are involved in the initial oxidation of inactivated alkanes. Beta-carotene ketolase and beta-carotene hydroxylase are carotenoid biosynthetic enzymes for astaxanthin and zeaxanthin, respectively. This superfamily domain has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of these sequences also reveals three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXX(X)H, HXX(X)HH, and HXXHH (an additional conserved histidine residue is seen between clusters 2 and 3). Spectroscopic and genetic evidence point to a nitrogen-rich coordination environment located in the cytoplasm with as many as eight histidines coordinating the two iron ions and a carboxylate residue bridging the two metals in the Pseudomonas oleovorans alkane hydroxylase (AlkB). In addition, the eight histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the rat stearoyl CoA delta-9 desaturase.


Pssm-ID: 238511 [Multi-domain]  Cd Length: 122  Bit Score: 65.57  E-value: 3.81e-13
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958642903 263 VLAALILAISQAQCWCLQHDLGHASIFPKSRWNHVAQQFvMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTV 336
Cdd:cd01060     2 LLALLLGLLGGLGLTVLAHELGHRSFFRSRWLNRLLGAL-LGLALGGSYGWWRRSHRRHHRYTNTPGKDPDSAV 74
PLN02252 PLN02252
nitrate reductase [NADPH]
 121-206 1.38e-09

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 59.69  E-value: 1.38e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 121 IFRWEQIRQHDLPGDKWLVIERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLhfVRKFLKPLLIGELAPEEP 200
Cdd:PLN02252  519 QYTMSEVRKHNSEDSCWIVVHGHVYDCTRFLKDHPGGADSILINAGTDCTEEFDAIHSDK--AKKMLEDYRIGELVTTGA 596


                  ....*.
gi 1958642903 201 SQDGAQ 206
Cdd:PLN02252  597 AASSSA 602
FA_desaturase pfam00487
Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a ...
 258-333 1.17e-05

Fatty acid desaturase; Fatty acid desaturases are enzymes that catalyze the insertion of a double bond at the delta position of fatty acids. There seem to be two distinct families of fatty acid desaturases which do not seem to be evolutionary related: Family 1 composed of Stearoyl-CoA desaturases (SCD) and Family 2 composed of Bacterial fatty acid desaturases, Plant stearoyl-acyl-carrier-protein desaturase and Cyanobacterial DesA. Members of this entry are ER integral membrane proteins that share the same mushroom-shaped fold consisting of four transmembrane helices (TM1-TM4) which anchor them to the membrane, capped by a cytosolic domain containing a unique 9-10 histidine- coordinating di metal (di-iron) catalytic centre. The structure of mouse stearoyl-CoA desaturase (SDC) revealed that TM2 and TM4 are longer than TM1 and TM3 and protrude into the cytosolic domain, providing three of the nine histidine residues that coordinate the two metal ions, while the other histidine residues are provided by the soluble domain in this enzyme.


Pssm-ID: 425713 [Multi-domain]  Cd Length: 252  Bit Score: 46.19  E-value: 1.17e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1958642903 258 GWVSSVLAALILAISQAQCWCLQHDLGHASIFPK---SRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPD 333
Cdd:pfam00487   1 SWLALLLALLLGLFLLGITGSLAHEASHGALFKKrrlNRWLNDLLGRLAGLPLGISYSAWRIAHLVHHRYTNGPDKDPD 79
Delta12-FADS-like cd03507
The Delta12 Fatty Acid Desaturase (Delta12-FADS)-like CD includes the integral-membrane ...
 259-339 4.59e-05

The Delta12 Fatty Acid Desaturase (Delta12-FADS)-like CD includes the integral-membrane enzymes, delta-12 acyl-lipid desaturases, oleate 12-hydroxylases, omega3 and omega6 fatty acid desaturases, and other related proteins, found in a wide range of organisms including higher plants, green algae, diatoms, nematodes, fungi, and bacteria. The expression of these proteins appears to be temperature dependent: decreases in temperature result in increased levels of fatty acid desaturation within membrane lipids subsequently altering cell membrane fluidity. An important enzyme for the production of polyunsaturates in plants is the oleate delta-12 desaturase (Arabidopsis FAD2) of the endoplasmic reticulum. This enzyme accepts l-acyl-2-oleoyl-sn-glycero-3-phosphocholine as substrate and requires NADH:cytochrome b oxidoreductase, cytochrome b, and oxygen for activity. FAD2 converts oleate(18:1) to linoleate (18:2) and is closely related to oleate 12-hydroxylase which catalyzes the hydroxylation of oleate to ricinoleate. Plastid-bound desaturases (Arabidopsis delta-12 desaturase (FAD6), omega-3 desaturase (FAD8), omega-6 desaturase (FAD6)), as well as, the cyanobacterial thylakoid-bound FADSs require oxygen, ferredoxin, and ferredoxin oxidoreductase for activity. As in higher plants, the cyanobacteria delta-12 (DesA) and omega-3 (DesB) FADSs desaturate oleate (18:1) to linoleate (18:2) and linoleate (18:2) to linolenate (18:3), respectively. Omega-3 (DesB/FAD8) and omega-6 (DesD/FAD6) desaturases catalyze reactions that introduce a double bond between carbons three and four, and carbons six and seven, respectively, from the methyl end of fatty acids. As with other members of this superfamily, this domain family has extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH, HXX(X)HH, and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within the homologue, stearoyl CoA desaturase. Mutation of any one of four of these histidines in the Synechocystis delta-12 acyl-lipid desaturase resulted in complete inactivity.


Pssm-ID: 239584 [Multi-domain]  Cd Length: 222  Bit Score: 44.14  E-value: 4.59e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 259 WVSSVLAALILAISQA----QCWCLQHDLGHASIFPKSRWNHVAqQFVMGQLKGFSAHWWNFRHFQHHAKPNifHKDPDV 334
Cdd:cd03507    26 LLLSWWLWPLYWIVQGlfltGLFVLGHDCGHGSFSDNRRLNDIV-GHILHSPLLVPYHSWRISHNRHHAHTG--NLEGDE 102


                  ....*
gi 1958642903 335 TVAPV 339
Cdd:cd03507   103 VWVPV 107
Rhizopine-oxygenase-like cd03511
This CD includes the putative hydrocarbon oxygenase, MocD, a bacterial rhizopine ...
 258-338 4.81e-05

This CD includes the putative hydrocarbon oxygenase, MocD, a bacterial rhizopine (3-O-methyl-scyllo-inosamine, 3-O-MSI) oxygenase, and other related proteins. It has been proposed that MocD, MocE (Rieske-like ferredoxin), and MocF (ferredoxin reductase) under the regulation of MocR, act in concert to form a ferredoxin oxygenase system that demethylates 3-O-MSI to form scyllo-inosamine. This domain family appears to be structurally related to the membrane fatty acid desaturases and the alkane hydroxylases. They all share in common extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH, HXXHH, and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within homologs, stearoyl CoA desaturase and alkane hydroxylase.


Pssm-ID: 239588 [Multi-domain]  Cd Length: 285  Bit Score: 44.28  E-value: 4.81e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 258 GWVSSVLAALILAISQAQCWCLQHDLGHASIFpKSRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVA 337
Cdd:cd03511    40 GSWWALPAFLVYGVLYAALFARWHECVHGTAF-ATRWLNDAVGQIAGLMILLPPDFFRWSHARHHRYTQIPGRDPELAVP 118


                  .
gi 1958642903 338 P 338
Cdd:cd03511   119 R 119
Rhizobitoxine-FADS-like cd03510
This CD includes the dihydrorhizobitoxine fatty acid desaturase (RtxC) characterized in ...
 256-342 1.97e-03

This CD includes the dihydrorhizobitoxine fatty acid desaturase (RtxC) characterized in Bradyrhizobium japonicum USDA110, and other related proteins. Dihydrorhizobitoxine desaturase is reported to be involved in the final step of rhizobitoxine biosynthesis. This domain family appears to be structurally related to the membrane fatty acid desaturases and the alkane hydroxylases. They all share in common extensive hydrophobic regions that would be capable of spanning the membrane bilayer at least twice. Comparison of sequences also reveals the existence of three regions of conserved histidine cluster motifs that contain eight histidine residues: HXXXH, HXX(X)HH, and HXXHH. These histidine residues are reported to be catalytically essential and proposed to be the ligands for the iron atoms contained within homologs, stearoyl CoA desaturase and alkane hydroxylase.


Pssm-ID: 239587 [Multi-domain]  Cd Length: 175  Bit Score: 38.80  E-value: 1.97e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958642903 256 GPGWVSSVLAALILAISQAQCWCLQHDLGHASIFPKSRWNHVAQQFVMGQLKGFSAHWWNFRHFQHHAKPNIfHKDPDVT 335
Cdd:cd03510    15 WPNWLAYLLAVLLIGARQRALAILMHDAAHGLLFRNRRLNDFLGNWLAAVPIFQSLAAYRRSHLKHHRHLGT-EDDPDLA 93


                  ....*..
gi 1958642903 336 VAPVFLL 342
Cdd:cd03510    94 LYLLLWL 100
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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