|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
50-430 |
5.19e-177 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 510.86 E-value: 5.19e-177
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVT 129
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEDLTPFRDMSKKVY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 130 ELLEEFSPA--VERLGFDENFVDLTEMvekrlqqlpsdevpsvtmsghvynnqsvnlhssthvRLAVGSQIAAEMREAMH 207
Cdd:cd01703 81 RLLRSYSWNdrVERLGFDENFMDVTEM------------------------------------RLLVASHIAYEMRERIE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 208 NQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIKEMPGIGYKTAKRLEVLGINSVHDLQTFPI 285
Cdd:cd01703 125 NELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPPSCADLMdfMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 286 ---------------KTLEKELGISVAQRIRKLSFGEDKSPV-TPSGPPQSFSEEDTFKKCSSEV--EAKTKIEELLSSL 347
Cdd:cd01703 205 rnrqtvgaapsllelLLMVKEFGEGIGQRIWKLLFGRDTSPVkPASDFPQQISIEDSYKKCSLEEirEARNKIEELLASL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 348 LNRVC--------HDGRKPHTIRLVIRRYSD--KHCNRESRQCPIPSHVIQKLGTGNYDAVPPLMEILMKLFRNMVNVK- 416
Cdd:cd01703 285 LERMKqdlqevkaGDGRRPHTLRLTLRRYTStkKHYNRESKQAPIPSHVFQKLTGGNEIAARPIEKILMRLFRELVPPKn 364
|
410
....*....|....*
gi 1958742570 417 -MAFHLTLLSVCFCN 430
Cdd:cd01703 365 vKGFNLTLLNVCFTN 379
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
47-385 |
5.69e-80 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 258.92 E-value: 5.69e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 47 RVIVHVDLDCFYAQVEMISNPELKDKPLGV---QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYRE 123
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVggdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPP-DFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 124 MSYKVTELLEEFSPAVERLGFDENFVDLTEMvekrlQQLPSDEVpsvtmsghvynnqsvnlhssthvrlavgsQIAAEMR 203
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGS-----ARLFGSAE-----------------------------AIARRIR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 204 EAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEMPGIGYKTAKRLEVLGINSVHDLQTF 283
Cdd:COG0389 126 RRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAFLAPL-PVEKLWGVGPKTAEKLARLGIRTIGDLAAL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 284 PIKTLEKELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKKcssEVEAKTKIEELLSSLLNRVCHD----GRKPH 359
Cdd:COG0389 205 PRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVERTFGE---DLTDLEELEAALRRLAERLAERlrrqGLGAR 280
|
330 340
....*....|....*....|....*.
gi 1958742570 360 TIRLVIrRYSDKHCNRESRQCPIPSH 385
Cdd:COG0389 281 TVTVKL-RTSDFRTTTRSRTLPEPTD 305
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
55-370 |
2.13e-59 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 204.20 E-value: 2.13e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 55 DCFYAQVEMISNPELKDKPLGV----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYREMSYKVTE 130
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVggspGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPG-RFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 131 LLEEFSPAVERLGFDENFVDLTEmvekrlqqlpsdevpsvtmsghvynnqSVNLHSSthvrlavGSQIAAEMREAMHNQL 210
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTD---------------------------NKLCIGS-------ATLIAQEIRQDIFEEL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 211 GLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEK 290
Cdd:PRK02406 127 GLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFLATLP-VEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 291 ELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKTKIEEL---LSSLLNRVCHDgRKPHTIRLVIr 366
Cdd:PRK02406 206 HFG-KFGRRLYERARGIDERPVKPDRERKSVGVERTFAEdLYDLEACLAELPRLaekLERRLERAKPD-KRIKTVGVKL- 282
|
....
gi 1958742570 367 RYSD 370
Cdd:PRK02406 283 KFAD 286
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
52-229 |
1.61e-45 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 159.28 E-value: 1.61e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 52 VDLDCFYAQVEMISNPELKDKPLGVQQKY---LVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVnGEDLSRYREMSYKV 128
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNgrgIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-PPDLELYRRASRKI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 129 TELLEEF-SPAVERLGFDENFVDLTEMVEkrlqqlpsdevpsvtmsghvynnqsvnlhssthvRLAVGSQIAAEMREAMH 207
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEK----------------------------------LFGAEEALAKRLRREIA 125
|
170 180
....*....|....*....|..
gi 1958742570 208 NQLGLTGCAGVAPNKLLAKLVS 229
Cdd:pfam00817 126 EETGLTCSIGIAPNKLLAKLAS 147
|
|
| Rev1_UBM2 |
cd19318 |
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, ... |
696-721 |
8.96e-04 |
|
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, the second ubiquitin-binding motif of Rev1, a DNA damage tolerance protein. Rev1 acts as a translesion synthesis (TLS) DNA polymerase and may also recruit other TLS polymerases to the site of DNA damage; in that process the UBMs are essential for Rev1 function, triggering TLS activation via recognition of ubiquitin moieties in PCNA, the proliferating cell nuclear antigen.
Pssm-ID: 412037 Cd Length: 36 Bit Score: 37.20 E-value: 8.96e-04
10 20
....*....|....*....|....*.
gi 1958742570 696 PSDIDPQVFYELPEEVQRELMAEWKR 721
Cdd:cd19318 9 FSQVDPSVLAALPPDLQEELEAAYAQ 34
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
50-430 |
5.19e-177 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 510.86 E-value: 5.19e-177
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVT 129
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEDLTPFRDMSKKVY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 130 ELLEEFSPA--VERLGFDENFVDLTEMvekrlqqlpsdevpsvtmsghvynnqsvnlhssthvRLAVGSQIAAEMREAMH 207
Cdd:cd01703 81 RLLRSYSWNdrVERLGFDENFMDVTEM------------------------------------RLLVASHIAYEMRERIE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 208 NQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIKEMPGIGYKTAKRLEVLGINSVHDLQTFPI 285
Cdd:cd01703 125 NELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPPSCADLMdfMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 286 ---------------KTLEKELGISVAQRIRKLSFGEDKSPV-TPSGPPQSFSEEDTFKKCSSEV--EAKTKIEELLSSL 347
Cdd:cd01703 205 rnrqtvgaapsllelLLMVKEFGEGIGQRIWKLLFGRDTSPVkPASDFPQQISIEDSYKKCSLEEirEARNKIEELLASL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 348 LNRVC--------HDGRKPHTIRLVIRRYSD--KHCNRESRQCPIPSHVIQKLGTGNYDAVPPLMEILMKLFRNMVNVK- 416
Cdd:cd01703 285 LERMKqdlqevkaGDGRRPHTLRLTLRRYTStkKHYNRESKQAPIPSHVFQKLTGGNEIAARPIEKILMRLFRELVPPKn 364
|
410
....*....|....*
gi 1958742570 417 -MAFHLTLLSVCFCN 430
Cdd:cd01703 365 vKGFNLTLLNVCFTN 379
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
50-429 |
9.07e-114 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 347.04 E-value: 9.07e-114
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGVQQKY----LVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLSRYREMS 125
Cdd:cd00424 1 LHIDFDNFFASVEQLARPELKGRPVVVVPFNsdstCVIACSYEARKYGVKRGMPVREARKMCPNLILVP-ARLDLYRRLS 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 126 YKVTELLEEFSPAVERLGFDENFVDLTEMVEKrlqqlpsdevpsvtmsghvynnqsvnlhssthvrLAVGSQIAAEMREA 205
Cdd:cd00424 80 ERLLSELEEVAPLVEVASIDELFLDLTGSARL----------------------------------LGLGSEVALRIKRH 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 206 MHNQLG-LTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIhSLNHIKEMPGIGYKTAKRLEVLGINSVHDLQTFP 284
Cdd:cd00424 126 IAEQLGgITASIGIASNKLLAKLAAKYAKPDGLTILDPEDLPGFL-SKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAAS 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 285 IKTLEKELGISVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVE-AKTKIEELLSSLLNRVCHDGRKPHTIRL 363
Cdd:cd00424 205 PDALLALWGGVSGERLWYALRGIDDEPLSPPRPRKSFSHERVLPRDSRNAEdARPLLRLLLEKLARRLRRDGRGATRLRL 284
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1958742570 364 VIRRYSD---KHCNRESRQCPIPSHViqklgtgnydAVPPLMEILMKLFRNMVNVKMAFHLTLLSVCFC 429
Cdd:cd00424 285 WLRTVDGrwsGHADIPSRSAPRPIST----------EDGELLHALDKLWRALLDDKGPRRLRRLGVRLS 343
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
50-412 |
3.63e-81 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 261.69 E-value: 3.63e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGV---QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYREMSY 126
Cdd:cd03586 1 IHIDMDAFYASVEQRDNPELKGKPVAVggsSDRGVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPP-RFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 127 KVTELLEEFSPAVERLGFDENFVDLTEMVekrlqqlpsdevpsvtmsghvynnqsVNLHSSTHvrlavgsqIAAEMREAM 206
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLDVTDYV--------------------------RLFGSATE--------IAKEIRARI 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 207 HNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEMPGIGYKTAKRLEVLGINSVHDLQTFPIK 286
Cdd:cd03586 126 REETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPEDVEEFLAPL-PVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVE 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 287 TLEKELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTF-KKCSSEVEAKTKIEELLSSLLNRVCHDGRKPHTIRLVI 365
Cdd:cd03586 205 LLKKLFG-KSGRRLYELARGIDNRPVEPDRERKSIGVERTFsEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKL 283
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 1958742570 366 rRYSDKHCNreSRQCPIPSHviqklgTGNYDAvppLMEILMKLFRNM 412
Cdd:cd03586 284 -KYADFSTR--TRSRTLPEP------TDDAED---IYELALELLEEL 318
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
47-385 |
5.69e-80 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 258.92 E-value: 5.69e-80
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 47 RVIVHVDLDCFYAQVEMISNPELKDKPLGV---QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYRE 123
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVggdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPP-DFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 124 MSYKVTELLEEFSPAVERLGFDENFVDLTEMvekrlQQLPSDEVpsvtmsghvynnqsvnlhssthvrlavgsQIAAEMR 203
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGS-----ARLFGSAE-----------------------------AIARRIR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 204 EAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEMPGIGYKTAKRLEVLGINSVHDLQTF 283
Cdd:COG0389 126 RRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAFLAPL-PVEKLWGVGPKTAEKLARLGIRTIGDLAAL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 284 PIKTLEKELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKKcssEVEAKTKIEELLSSLLNRVCHD----GRKPH 359
Cdd:COG0389 205 PRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVERTFGE---DLTDLEELEAALRRLAERLAERlrrqGLGAR 280
|
330 340
....*....|....*....|....*.
gi 1958742570 360 TIRLVIrRYSDKHCNRESRQCPIPSH 385
Cdd:COG0389 281 TVTVKL-RTSDFRTTTRSRTLPEPTD 305
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
55-370 |
2.13e-59 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 204.20 E-value: 2.13e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 55 DCFYAQVEMISNPELKDKPLGV----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYREMSYKVTE 130
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVggspGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPG-RFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 131 LLEEFSPAVERLGFDENFVDLTEmvekrlqqlpsdevpsvtmsghvynnqSVNLHSSthvrlavGSQIAAEMREAMHNQL 210
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTD---------------------------NKLCIGS-------ATLIAQEIRQDIFEEL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 211 GLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEK 290
Cdd:PRK02406 127 GLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFLATLP-VEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 291 ELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKTKIEEL---LSSLLNRVCHDgRKPHTIRLVIr 366
Cdd:PRK02406 206 HFG-KFGRRLYERARGIDERPVKPDRERKSVGVERTFAEdLYDLEACLAELPRLaekLERRLERAKPD-KRIKTVGVKL- 282
|
....
gi 1958742570 367 RYSD 370
Cdd:PRK02406 283 KFAD 286
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
50-430 |
5.28e-56 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 195.61 E-value: 5.28e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLV------NGED------ 117
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNSIIAVSYAARAFGVTRFMTIDEAKKKCPDLILAhvatykKGEDeadyhe 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 118 ----------LSRYREMSYKVTELLEEFSPAVERLGFDENFVDLtemvekrlqqlpsdevpsvtmsghvynnqsvnlhss 187
Cdd:cd01702 81 npsparhkvsLDPYRRASRKILNILKRFGDVVEKASIDEAYLDL------------------------------------ 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 188 thvrlavGSQIAAEMREAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAK 267
Cdd:cd01702 125 -------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLP-ITSIRGLGGKLGE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 268 RL-EVLGINSVHDLQTFPIKT--LEKELGISVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTF--KKCSSEVEAKTKIEE 342
Cdd:cd01702 197 EIiDLLGLPTEGDVAGFRSSEsdLQEHFGEKLGEWLYNLLRGIDHEPVKPRPLPKSMGSSKNFpgKTALSTEDVQHWLLV 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 343 LLSSLLNRVCHD----GRKPHTIRLVIRRYSDKhcNRESRQCPIPSHVIQKLgtgnydaVPPLMEILMKLFRNMVNVKMA 418
Cdd:cd01702 277 LASELNSRLEDDryenNRRPKTLVLSLRQRGDG--VRRSRSCALPRYDAQKI-------VKDAFKLIKAINEEGLGLAWN 347
|
410
....*....|..
gi 1958742570 419 FHLTLLSVCFCN 430
Cdd:cd01702 348 YPLTLLSLSFTK 359
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
46-370 |
6.33e-54 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 191.40 E-value: 6.33e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 46 SRVIVHVDLDCFYAQVEMISNPELKDKPLGV-----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLSR 120
Cdd:PRK01810 4 GRVIFHVDMNSFFASVEIAYDPSLQGKPLAVagnekERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRR-PNFDR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 121 YREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKrlqqlpsdevpsvtmsGHVYnnqsvnlhssthvrlavgsQIAA 200
Cdd:PRK01810 83 YREASRQMFQILSEFTPLVQPVSIDEGYLDITDCYAL----------------GSPL-------------------EIAK 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 201 EMREAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEMPGIGYKTAKRLEVLGINSVHDL 280
Cdd:PRK01810 128 MIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRKRDVPEMLWPL-PVGEMHGIGEKTAEKLKDIGIQTIGDL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 281 QTFPIKTLEKELGISvAQRIRKLSFGEDKSPVTPS--------GPPQSFSEE--------DTFKKCSSEVEAKTKIEELL 344
Cdd:PRK01810 207 AKADEHILRAKLGIN-GVRLQRRANGIDDRPVDPEaiyqfksvGNSTTLSHDmdeekellDVLRRLSKSVSKRLQKKTVV 285
|
330 340
....*....|....*....|....*.
gi 1958742570 345 SSllnrvchdgrkphTIRLVIrRYSD 370
Cdd:PRK01810 286 SY-------------NVQIMI-RYHD 297
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
52-229 |
1.61e-45 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 159.28 E-value: 1.61e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 52 VDLDCFYAQVEMISNPELKDKPLGVQQKY---LVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVnGEDLSRYREMSYKV 128
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNgrgIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-PPDLELYRRASRKI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 129 TELLEEF-SPAVERLGFDENFVDLTEMVEkrlqqlpsdevpsvtmsghvynnqsvnlhssthvRLAVGSQIAAEMREAMH 207
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEK----------------------------------LFGAEEALAKRLRREIA 125
|
170 180
....*....|....*....|..
gi 1958742570 208 NQLGLTGCAGVAPNKLLAKLVS 229
Cdd:pfam00817 126 EETGLTCSIGIAPNKLLAKLAS 147
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
43-384 |
6.48e-41 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 154.78 E-value: 6.48e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 43 IASSRVIVHVDLDCFYAQVEMISNPELKDKPLGV-QQKYL---VVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVnGEDL 118
Cdd:cd01701 43 PDLQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVcHGKGPnseIASCNYEARSYGIKNGMWVGQAKKLCPQLVTL-PYDF 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 119 SRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEmvekrlqqlPSDEVPSVTMsghvynnqsvnlhssthvRLAvgSQI 198
Cdd:cd01701 122 EAYEEVSLTFYEILASYTDNIEAVSCDEALIDITS---------LLEETYELPE------------------ELA--EAI 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 199 AAEMREAmhnqlglTGC---AGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGIN 275
Cdd:cd01701 173 RNEIRET-------TGCsasVGIGPNILLARLATRKAKPDGQYHLSAEKVEEFLSQLK-VGDLPGVGSSLAEKLVKLFGD 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 276 SVHD--LQTFPIKTLEKELGISVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDT----FKKcssEVEAKTKIEELLSSLLN 349
Cdd:cd01701 245 TCGGleLRSKTKEKLQKVLGPKTGEKLYDYCRGIDDRPVTGEKERKSVSAEINygirFTN---VDDVEQFLQRLSEELSK 321
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 1958742570 350 RVCHDGRKPHTIRLVIRRYSDKH------------CNRESRQCPIPS 384
Cdd:cd01701 322 RLEESNVTGRQITLKLMKRAPGApieppkymghgiCDSFSKSSTLGV 368
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
44-368 |
1.11e-40 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 155.09 E-value: 1.11e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 44 ASSRVIVHVDLDCFYAQVEMISNPELKDKPL---GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLSR 120
Cdd:PRK03348 2 RAQRWVLHLDMDAFFASVEQLTRPTLRGRPVlvgGLGGRGVVAGASYEARVFGARSAMPMHQARRLVGNGAVVLPPRFVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 121 YREMSYKVTELLEEFSPAVERLGFDENFVDLTEmvekrLQQLPSDEVpsvtmsghvynnqsvnlhssthvrlavgSQIAA 200
Cdd:PRK03348 82 YRAASRRVFDTLRELSPVVEQLSFDEAFVEPAE-----LAGASAEEV----------------------------EAFAE 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 201 EMREAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDL 280
Cdd:PRK03348 129 RLRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIRVVPPGEERELLAPLP-VRRLWGIGPVTEEKLHRLGIETIGDL 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 281 QTFPIKTLEKELGISVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKTKIEELLSSLLNRVCHDGRKPH 359
Cdd:PRK03348 208 AALSEAEVANLLGATVGPALHRLARGIDDRPVAERAEAKQISAESTFAVdLTTRAQLREAIERIAEHAHRRLLKDGRGAR 287
|
....*....
gi 1958742570 360 TIRLVIRRY 368
Cdd:PRK03348 288 TVTVKLRKS 296
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
47-348 |
4.61e-40 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 150.64 E-value: 4.61e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 47 RVIVHVDLDCFYAQVEMISNPELKDKPL---GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLSRYRE 123
Cdd:PRK14133 3 RVIIHVDMDAFFASVEQMDNPKLKGKPVivgGISERGVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVR-HERYKE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 124 MSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKrlqqlpsdevpsvtmsghvynnqsvnlhssthvrlavGSQIAAEMR 203
Cdd:PRK14133 82 VSKNIFKILYEVTPIVEPVSIDEAYLDITNIKEE-------------------------------------PIKIAKYIK 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 204 EAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDLQTF 283
Cdd:PRK14133 125 KKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKIITEDMIPDILKPLP-ISKVHGIGKKSVEKLNNIGIYTIEDLLKL 203
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1958742570 284 PIKTLEK---ELGISVAQRIRklsfGEDKSPVTPSGPPQSFSEEDTFKKcsseveaKTKIEELLSSLL 348
Cdd:PRK14133 204 SREFLIEyfgKFGVEIYERIR----GIDYREVEVSRERKSIGKETTLKK-------DTKDKEELKKYL 260
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
49-327 |
1.29e-38 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 148.54 E-value: 1.29e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 49 IVHVDLDCFYAQVEMISNPELKDKPL--GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLSRYREMSY 126
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPViiGGGKRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKP-DMEKYVRVGR 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 127 KVTELLEEFSPAVERLGFDENFVDL--TEmvekRLQQLPsdevPSVTMSghvynnqsvnlhssthvRLAvgsqiaaemrE 204
Cdd:PRK02794 117 EVRAMMQALTPLVEPLSIDEAFLDLsgTE----RLHGAP----PAVVLA-----------------RFA----------R 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 205 AMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIkemPGIGYKTAKRLEVLGINSVHDLQT 282
Cdd:PRK02794 162 RVEREIGITVSVGLSYNKFLAKIASDLDKPRGFSVIGRAEALAFLapKPVGII---WGVGPATAARLARDGIRTIGDLQR 238
|
250 260 270 280
....*....|....*....|....*....|....*....|....*
gi 1958742570 283 FPIKTLEKELGiSVAQRIRKLSFGEDKSPVTPSGPPQSFSEEDTF 327
Cdd:PRK02794 239 ADEADLMRRFG-SMGLRLWRLARGIDDRKVSPDREAKSVSAETTF 282
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
47-315 |
1.56e-36 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 142.06 E-value: 1.56e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 47 RVIVHVDLDCFYAQVEMISNPELKDKPLGV-----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLSRY 121
Cdd:PRK03103 3 RVILLVDMQSFYASVEKAANPELKGRPVIVsgdpeRRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPR-MQRY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 122 REMSYKVTELLEEFSPAVERLGFDENFVDLTemvekrlqqlpsdevpsvtmsghvynnQSVNLHSSTHvrlavgsQIAAE 201
Cdd:PRK03103 82 IDVSLQITRILEDFTDLVEPFSIDEQFLDVT---------------------------GSQKLFGSPL-------EIAQK 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 202 MREAMHNQLGLTGCAGVAPNKLLAKLVSGVF---KPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVH 278
Cdd:PRK03103 128 IQQRIMRETGVYARVGIGPNKLLAKMACDNFakkNPDGLFTLDKEDVPADLWPLP-VRKLFGVGSRMEKHLRRMGIRTIG 206
|
250 260 270
....*....|....*....|....*....|....*..
gi 1958742570 279 DLQTFPIKTLEKELGIsVAQRIRKLSFGEDKSPVTPS 315
Cdd:PRK03103 207 QLANTPLERLKKRWGI-NGEVLWRTANGIDYSPVTPH 242
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
52-410 |
4.53e-35 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 136.52 E-value: 4.53e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 52 VDLDCFYAQVEMISNPELKDKPLGVqqkyL------VVTCNYEARKLGVKKLMNVRDAKEKCPQL-VLVNGEDLSRYREM 124
Cdd:cd01700 3 VDCNSFYASCERVFRPLLLGRPLVV----LsnndgcVIARSPEAKALGIKMGSPYFKVPDLLERHgVAVFSSNYALYGDM 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 125 SYKVTELLEEFSPAVERLGFDENFVDLTEMvekrlqqlpsdevpsvtmsghvynnqsvnLHSSTHVrlavgsQIAAEMRE 204
Cdd:cd01700 79 SRRIMSILERFSPDVEVYSIDESFLDLTGS-----------------------------LRFGDLE------ELARKIRR 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 205 AMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQT----VLLPESCQHLIHSLNHIKEMPGIGYKTAKRLEVLGINSVHDL 280
Cdd:cd01700 124 RILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYggvvDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 281 QTFPIKTLEKELGISVAQRIRKLSfGEDKSPVTPSGPP-QSFseedtfkkCSSE-----VEAKTKIEELLSSLLNRVCHD 354
Cdd:cd01700 204 AQADPDLLRKKFGVVGERLVRELN-GIDCLPLEEYPPPkKSI--------GSSRsfgrdVTDLDELKQALAEYAERAAEK 274
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 355 GRKPH----TIRLVIRrysdkhCNRESRQCPIPSHVIQKLGTGNYdavppLMEILMKLFR 410
Cdd:cd01700 275 LRRQKsvarTISVFIG------TSGFSRQPKYYSATNTLPYPTND-----TREIVKAALR 323
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
49-312 |
7.39e-35 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 137.04 E-value: 7.39e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 49 IVHVDLDCFYAQVEMISNPELKDKPL----GVqqkylVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLSRYREM 124
Cdd:PRK03858 6 ILHADLDSFYASVEQRDDPALRGRPVivggGV-----VLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPR-MSAYSRA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 125 SYKVTELLEEFSPAVERLGFDENFVDLTEMveKRLQQLPsdevpsvtmsghvynnqsvnlhssthvrlavgSQIAAEMRE 204
Cdd:PRK03858 80 SKAVFEVFRDTTPLVEGLSIDEAFLDVGGL--RRISGTP--------------------------------VQIAARLRR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 205 AMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDLQTFP 284
Cdd:PRK03858 126 RVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRELAFLHPLP-VRRLWGVGPVTAAKLRAHGITTVGDVAELP 204
|
250 260
....*....|....*....|....*...
gi 1958742570 285 IKTLEKELGISVAQRIRKLSFGEDKSPV 312
Cdd:PRK03858 205 ESALVSLLGPAAGRHLHALAHNRDPRRV 232
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
48-314 |
2.81e-34 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 134.15 E-value: 2.81e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 48 VIVHVDLDCFYAQVEMISNPELKDKPLGV-------QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDlSR 120
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVcvysgrfEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRK-EV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 121 YREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEkrlqqlpsdevpsvtmsghvyNNQSvnlhssthvrlavGSQIAA 200
Cdd:PRK01216 81 YQQVSNRIMKLLREYSEKIEIASIDEAYLDISDKVK---------------------NYQD-------------AYNLGL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 201 EMREAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDL 280
Cdd:PRK01216 127 EIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRFINELD-IADIPGIGDITAEKLKKLGVNKLVDT 205
|
250 260 270
....*....|....*....|....*....|....
gi 1958742570 281 QTFPIKTLEKELGISVAQRIRKLSFGEDKSPVTP 314
Cdd:PRK01216 206 LRIEFDELKGIIGEAKAKYLFSLARNEYNEPVRA 239
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
43-384 |
2.09e-32 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 128.60 E-value: 2.09e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 43 IASSRVIVHVDLDCFYAQVEMISNPELKDKPLGV------QQKYLVVTC-NYEARKLGVKKLMNVRDAKEKCPQLVLVNg 115
Cdd:PRK03352 1 TAMPRWVLHVDLDQFIAAVELLRRPELAGLPVIVggngdpTEPRKVVTCaSYEARAFGVRAGMPLRTAARRCPDAVFLP- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 116 EDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDltemvekrlqqlpsdevpsvtmsghvynnqsvnlhssthVRLAVG 195
Cdd:PRK03352 80 SDPAAYDAASEEVMATLRDLGVPVEVWGWDEAFLG---------------------------------------VDTDDP 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 196 SQIAAEMREAMHNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGIN 275
Cdd:PRK03352 121 EALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRLTDANWMAVMGDRP-TDALWGVGPKTAKRLAALGIT 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 276 SVHDLQTFPIKTLEKELGISVAQRIRKLSFGEDKSPVTpSGP--PQSFSEEDTFKK---CSSEVEAktKIEELLSSLLNR 350
Cdd:PRK03352 200 TVADLAAADPAELAATFGPTTGPWLLLLARGGGDTEVS-AEPwvPRSRSREVTFPQdltDRAEVES--AVRELARRVLDE 276
|
330 340 350
....*....|....*....|....*....|....*
gi 1958742570 351 VCHDGRKPhtIRLVIR-RYSDKHCNRESRQCPIPS 384
Cdd:PRK03352 277 VVAEGRPV--TRVAVKvRTATFYTRTKIRKLPEPT 309
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
50-280 |
1.26e-19 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 93.16 E-value: 1.26e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 50 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLSRYREMSYKVT 129
Cdd:PTZ00205 136 IHLDMDMFYAAVEIKKHPEYAAIPLAIGTMTMLQTANYVARGRGIRQGMPGFLALKICPNLLILP-PDFDAYNEESNTVR 214
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 130 ELLEEFSPAVERLGFDENFVDLTEMVEkRLQQLPSDEvpsvtmsghvynnqsvnlhssthvrlavgsQIAAEMREAMHNQ 209
Cdd:PTZ00205 215 RIVAEYDPNYISFGLDELTLEVSAYIE-RFEGTKTAE------------------------------DVASELRVRVFGE 263
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958742570 210 LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVL---LPESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGINSVHDL 280
Cdd:PTZ00205 264 TKLTASAGIGPTAALAKIASNINKPNGQHDLnlhTRGDVMTYVRDLG-LRSVPGVGKVTEALLKGLGITTLSDI 336
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
51-307 |
1.73e-16 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 82.51 E-value: 1.73e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 51 HVDLDCFYAQVEMISNPELKDKPLGV--QQKYLVVTCNYEARKLGVK---KLMNVRDAKEKCPQLVLVNGEDLsrYREMS 125
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVlsNNDGCVIARSAEAKALGIKmgdPWFKQKDLFRRCGVVCFSSNYEL--YADMS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 126 YKVTELLEEFSPAVERLGFDENFVDLTEMVEKRlqqlpsdevpSVTMSGHvynnqsvnlhssthvrlavgsqiaaEMREA 205
Cdd:PRK03609 82 NRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCR----------DLTDFGR-------------------------EIRAT 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 206 MHNQLGLTGCAGVAPNKLLAKLVSGVFKP-NQQT---VLL--PESCQHLIhSLNHIKEMPGIGYKTAKRLEVLGINSVHD 279
Cdd:PRK03609 127 VLQRTHLTVGVGIAQTKTLAKLANHAAKKwQRQTggvVDLsnLERQRKLL-SLQPVEEVWGVGRRISKKLNAMGIKTALD 205
|
250 260
....*....|....*....|....*...
gi 1958742570 280 LQTFPIKTLEKELGISVAQRIRKLSfGE 307
Cdd:PRK03609 206 LADTNIRFIRKHFNVVLERTVRELR-GE 232
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
67-412 |
2.46e-09 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 59.70 E-value: 2.46e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 67 PELKDKPLGV--QQKYLVVT-CNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLSRYREMSYKVTELLEEFSPAVERLG 143
Cdd:cd03468 18 PADDEAPLAVveRKKAGRILaCNAAARAAGVRPGMPLAEALALCPNLQVVE-YDPEADARALQELALWLLRFTPLVALDG 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 144 FDENFVDltemvekrlqqlpsdevpsVTMSGHVYNnqsvnlhssthvrlavGSQIAAEMREAMHNQLGLTGCAGVAPNKL 223
Cdd:cd03468 97 PDGLLLD-------------------VTGCLHLFG----------------GEDALAASLRAALATLGLSARAGIADTPG 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 224 LAKLVSgvFKPNQQTVLLPESCQHLIHSLNH-IKEMPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEKELGISVAQRIRK 302
Cdd:cd03468 142 AAWLLA--RAGGGRGVLRREALAAALVLLAPlPVAALRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQ 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 303 LsFGEDKSPVTPSGPPQSFS-------EEDTFKkcSSEVEAKTKIEELLSSLLNRvchdgrkphtiRLVIRRYSdkhcNR 375
Cdd:cd03468 220 A-YGRDPEPLLFSPPPPAFDfrlelqlEEPIAR--GLLFPLRRLLEQLCAFLALR-----------GLGARRLS----LT 281
|
330 340 350
....*....|....*....|....*....|....*..
gi 1958742570 376 ESRQCPIPSHVIQKLGTGNYDAvpplmEILMKLFRNM 412
Cdd:cd03468 282 LFREDGRVTRVLVGLARPSRDD-----LPLLRLLRER 313
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
318-431 |
9.80e-08 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 50.64 E-value: 9.80e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958742570 318 PQSFSEEDTF-KKCSSEVEAKTKIEELLSSLLNRVCHDGRKPHTIRLVIrRYSDKHcnRESRQCPIPSHViqklgtgnyD 396
Cdd:pfam11799 1 RKSIGAERTFgRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKI-RYSDFR--TITRSVTLPSPT---------D 68
|
90 100 110
....*....|....*....|....*....|....*
gi 1958742570 397 AVPPLMEILMKLFRNMVNVKmafHLTLLSVCFCNL 431
Cdd:pfam11799 69 DTDEIYRAALRLLRRLYRGR---PVRLLGVSLSNL 100
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
242-274 |
1.60e-05 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 42.00 E-value: 1.60e-05
10 20 30
....*....|....*....|....*....|...
gi 1958742570 242 PESCQHLIHSLNhIKEMPGIGYKTAKRLEVLGI 274
Cdd:pfam11798 1 PDDVPEFLWPLP-ISKIPGIGKKLAEKLKALGI 32
|
|
| Rev1_UBM2 |
cd19318 |
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, ... |
696-721 |
8.96e-04 |
|
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, the second ubiquitin-binding motif of Rev1, a DNA damage tolerance protein. Rev1 acts as a translesion synthesis (TLS) DNA polymerase and may also recruit other TLS polymerases to the site of DNA damage; in that process the UBMs are essential for Rev1 function, triggering TLS activation via recognition of ubiquitin moieties in PCNA, the proliferating cell nuclear antigen.
Pssm-ID: 412037 Cd Length: 36 Bit Score: 37.20 E-value: 8.96e-04
10 20
....*....|....*....|....*.
gi 1958742570 696 PSDIDPQVFYELPEEVQRELMAEWKR 721
Cdd:cd19318 9 FSQVDPSVLAALPPDLQEELEAAYAQ 34
|
|
| HHH_5 |
pfam14520 |
Helix-hairpin-helix domain; |
257-300 |
8.46e-03 |
|
Helix-hairpin-helix domain;
Pssm-ID: 434010 [Multi-domain] Cd Length: 57 Bit Score: 35.16 E-value: 8.46e-03
10 20 30 40
....*....|....*....|....*....|....*....|....*.
gi 1958742570 257 EMPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEK--ELGISVAQRI 300
Cdd:pfam14520 6 SISGIGPKTALALLSAGIGTVEDLAEADVDELAEipGIGEKTAQRI 51
|
|
| |
