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Conserved domains on  [gi|1958672601|ref|XP_038946733|]
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exonuclease 1 isoform X1 [Rattus norvegicus]

Protein Classification

exonuclease 1( domain architecture ID 10177090)

exonuclease 1 is a 5'->3' double-stranded DNA exonuclease that could act in a pathway that corrects mismatched base pairs; may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity

CATH:  3.40.50.1010|1.10.150.20
EC:  3.1.-.-
Gene Ontology:  GO:0006310|GO:0006298|GO:0003677|GO:0035312|GO:0008409|GO:0017108|GO:0046872
PubMed:  20298197|16905530

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PIN_EXO1 cd09857
FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' ...
 1-201 1.81e-122

FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR)), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. These nucleases are members of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


:

Pssm-ID: 350207 [Multi-domain]  Cd Length: 202  Bit Score: 367.11  E-value: 1.81e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGC 80
Cdd:cd09857     1 MGIQGLLPFLKPIQRPVHISEYAGKTVAVDAYCWLHRGAYSCAEELALGKPTDKYIDYCMKRVNMLLHHGITPILVFDGA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  81 TLPSKKEVERSRRERRQSNLLKGKQLLREGKVSEARECFTRSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNK 160
Cdd:cd09857    81 PLPSKAGTEEERRERREEALEKALELLREGKKSEARECFQRAVDITPEMAHELIKALRKENVEYIVAPYEADAQLAYLAK 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1958672601 161 AGIVQAVITEDSDLLAFGCKKVILKMDQFGNGLEVDQARLG 201
Cdd:cd09857   161 TGYVDAVITEDSDLLAFGCPKVLFKLDKDGNGQEIDRDDLG 201
H3TH_EXO1 cd09908
H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; ...
 213-289 1.65e-34

H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; Exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. Members of this subgroup include the H3TH (helix-3-turn-helix) domains of EXO1 and other similar eukaryotic 5' nucleases. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. These nucleases have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (Mg2+ or Mn2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


:

Pssm-ID: 188628 [Multi-domain]  Cd Length: 73  Bit Score: 125.77  E-value: 1.65e-34
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLANnpDIVKVIKKIGHYLKMniTVPEDYITGFIRANNTFLYQLVFD 289
Cdd:cd09908     1 EKFRHMCILSGCDYLPSLPGIGLKKAYKLVRRHR--TIEKVIKALRFDGKK--EVPPDYEEGFQKALLTFLHQRVFD 73
 
Name Accession Description Interval E-value
PIN_EXO1 cd09857
FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' ...
 1-201 1.81e-122

FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR)), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. These nucleases are members of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


Pssm-ID: 350207 [Multi-domain]  Cd Length: 202  Bit Score: 367.11  E-value: 1.81e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGC 80
Cdd:cd09857     1 MGIQGLLPFLKPIQRPVHISEYAGKTVAVDAYCWLHRGAYSCAEELALGKPTDKYIDYCMKRVNMLLHHGITPILVFDGA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  81 TLPSKKEVERSRRERRQSNLLKGKQLLREGKVSEARECFTRSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNK 160
Cdd:cd09857    81 PLPSKAGTEEERRERREEALEKALELLREGKKSEARECFQRAVDITPEMAHELIKALRKENVEYIVAPYEADAQLAYLAK 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1958672601 161 AGIVQAVITEDSDLLAFGCKKVILKMDQFGNGLEVDQARLG 201
Cdd:cd09857   161 TGYVDAVITEDSDLLAFGCPKVLFKLDKDGNGQEIDRDDLG 201
H3TH_EXO1 cd09908
H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; ...
 213-289 1.65e-34

H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; Exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. Members of this subgroup include the H3TH (helix-3-turn-helix) domains of EXO1 and other similar eukaryotic 5' nucleases. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. These nucleases have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (Mg2+ or Mn2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


Pssm-ID: 188628 [Multi-domain]  Cd Length: 73  Bit Score: 125.77  E-value: 1.65e-34
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLANnpDIVKVIKKIGHYLKMniTVPEDYITGFIRANNTFLYQLVFD 289
Cdd:cd09908     1 EKFRHMCILSGCDYLPSLPGIGLKKAYKLVRRHR--TIEKVIKALRFDGKK--EVPPDYEEGFQKALLTFLHQRVFD 73
PTZ00217 PTZ00217
flap endonuclease-1; Provisional
 1-295 1.92e-34

flap endonuclease-1; Provisional


Pssm-ID: 240317 [Multi-domain]  Cd Length: 393  Bit Score: 136.29  E-value: 1.92e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   1 MGIQGLLQFIKEASePVNVKK-----YKGQAVAVDTYCWLHKGAIACAEKL-------AKGEPTDRYVGFcMKFVNMLLS 68
Cdd:PTZ00217    1 MGIKGLSKFLADKA-PNAIKEqelknYFGRVIAIDASMALYQFLIAIRDDSqggnltnEAGEVTSHISGL-FNRTIRLLE 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  69 YGVKPILVFDGcTLPSKKeversrrerrQSNLLKGKQL-----------LREGKVSEARECFTRSVNITHAMAHKVIKAA 137
Cdd:PTZ00217   79 AGIKPVYVFDG-KPPELK----------SGELEKRRERreeaeeelekaIEEGDDEEIKKQSKRTVRVTKEQNEDAKKLL 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 138 RALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGNG----LEVDQARlgMCKQLGdvFTEE 213
Cdd:PTZ00217  148 RLMGIPVIEAPCEAEAQCAELVKKGKVYAVATEDMDALTFGTPVLLRNLNFSEAKkrpiQEINLST--VLEELG--LSMD 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 214 KFRYMCILSGCDYLASLRGIGLAKACKVLRLANNpdIVKVIKKIGhylKMNITVPEDYitGFIRANNTFLYQLVFDPIQR 293
Cdd:PTZ00217  224 QFIDLCILCGCDYCDTIKGIGPKTAYKLIKKYKS--IEEILEHLD---KTKYPVPENF--DYKEARELFLNPEVTPAEEI 296


                  ..
gi 1958672601 294 KL 295
Cdd:PTZ00217  297 DL 298
XPGN smart00485
Xeroderma pigmentosum G N-region; domain in nucleases
 1-86 1.07e-30

Xeroderma pigmentosum G N-region; domain in nucleases


Pssm-ID: 214690 [Multi-domain]  Cd Length: 99  Bit Score: 116.18  E-value: 1.07e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601    1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGC 80
Cdd:smart00485   1 MGIKGLWPLLKPVVREVPLEALRGKTLAIDASIWLYQFLTACREKLGTPLPNSKHLMGLFYRTCRLLEFGIKPIFVFDGK 80


                   ....*.
gi 1958672601   81 TLPSKK 86
Cdd:smart00485  81 PPPLKS 86
XPG_I pfam00867
XPG I-region;
141-225 1.51e-29

XPG I-region;


Pssm-ID: 459970 [Multi-domain]  Cd Length: 87  Bit Score: 112.22  E-value: 1.51e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 141 GVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGNG---LEVDQARLG-MCKQLGdvFTEEKFR 216
Cdd:pfam00867   1 GIPYVVAPGEAEAQCAYLQKSGLVDAVISEDSDVLLFGAPRLLRNLTGKSKKkskVPVEEIDLEkILKELG--LTREQLI 78


                  ....*....
gi 1958672601 217 YMCILSGCD 225
Cdd:pfam00867  79 DLAILLGCD 87
rad2 TIGR00600
DNA excision repair protein (rad2); All proteins in this family for which functions are known ...
 97-242 4.31e-07

DNA excision repair protein (rad2); All proteins in this family for which functions are known are flap endonucleases that generate the 3' incision next to DNA damage as part of nucleotide excision repair. This family is related to many other flap endonuclease families including the fen1 family. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273166 [Multi-domain]  Cd Length: 1034  Bit Score: 53.75  E-value: 4.31e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   97 QSNLLKGKQLLREGKVSEAREcftrSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLA 176
Cdd:TIGR00600  745 EANLLAEQNSLKAQKQQQKRI----AAEVTGQMILESQELLRLFGIPYIVAPMEAEAQCAILDLLDQTSGTITDDSDIWL 820

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958672601  177 FGCKKVILKMDQFGNGLEVDQArLGMCKQLGdvFTEEKFRYMCILSGCDYLASLRGIGLAKACKVL 242
Cdd:TIGR00600  821 FGARHVYKNFFNQNKFVEYYQY-VDIHNQLG--LDRNKLINLAYLLGSDYTEGIPTVGPVSAMEIL 883
HhH2 smart00279
Helix-hairpin-helix class 2 (Pol1 family) motifs;
213-243 8.06e-05

Helix-hairpin-helix class 2 (Pol1 family) motifs;


Pssm-ID: 197623 [Multi-domain]  Cd Length: 36  Bit Score: 40.51  E-value: 8.06e-05
                           10        20        30
                   ....*....|....*....|....*....|...
gi 1958672601  213 EKFRYMCILSG--CDYLASLRGIGLAKACKVLR 243
Cdd:smart00279   2 EQFIDYAILVGdySDNIPGVKGIGPKTALKLLR 34
 
Name Accession Description Interval E-value
PIN_EXO1 cd09857
FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' ...
 1-201 1.81e-122

FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR)), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. These nucleases are members of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


Pssm-ID: 350207 [Multi-domain]  Cd Length: 202  Bit Score: 367.11  E-value: 1.81e-122
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGC 80
Cdd:cd09857     1 MGIQGLLPFLKPIQRPVHISEYAGKTVAVDAYCWLHRGAYSCAEELALGKPTDKYIDYCMKRVNMLLHHGITPILVFDGA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  81 TLPSKKEVERSRRERRQSNLLKGKQLLREGKVSEARECFTRSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNK 160
Cdd:cd09857    81 PLPSKAGTEEERRERREEALEKALELLREGKKSEARECFQRAVDITPEMAHELIKALRKENVEYIVAPYEADAQLAYLAK 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1958672601 161 AGIVQAVITEDSDLLAFGCKKVILKMDQFGNGLEVDQARLG 201
Cdd:cd09857   161 TGYVDAVITEDSDLLAFGCPKVLFKLDKDGNGQEIDRDDLG 201
PIN_FEN1_EXO1-like cd00128
FEN-like PIN domains of Flap endonuclease-1 (FEN1)-like and exonuclease-1 (EXO1)-like ...
 6-202 4.76e-59

FEN-like PIN domains of Flap endonuclease-1 (FEN1)-like and exonuclease-1 (EXO1)-like nucleases, structure-specific, divalent-metal-ion dependent, 5' nucleases; PIN (PilT N terminus) domain of Flap endonuclease-1 (FEN1) and exonuclease-1 (EXO1)-like nucleases: FEN1, EXO1, Mkt1, Gap endonuclease 1 (GEN1) and Xeroderma pigmentosum complementation group G (XPG) nuclease. These nucleases are members of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350200 [Multi-domain]  Cd Length: 162  Bit Score: 197.98  E-value: 4.76e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   6 LLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGCTLPSK 85
Cdd:cd00128     1 LWQFIGEAKEPISIESLKGKTVAIDASIWVYQFLTAKREQGGDIGVTNSHLRGLFYRIIKLLSNGIKPIFVFDGGPPPLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  86 KEversrrerrqsnllkgkqllregkvsearecftrsvNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNKAGIVQ 165
Cdd:cd00128    81 KE------------------------------------TITKKMYQECKHLLSLFGIPYVVAPYEAEAQCAYLLKAGIVD 124

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1958672601 166 AVITEDSDLLAFGCKKVILKMDQFG-NGLEVDQARLGM 202
Cdd:cd00128   125 AAITEDSDCLLFGAPRVIRNMTFEGpHVEEFDASSILE 162
H3TH_EXO1 cd09908
H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; ...
 213-289 1.65e-34

H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease; Exonuclease-1 (EXO1) is involved in multiple, eukaryotic DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity), DNA repair processes (DNA mismatch repair (MMR) and post-replication repair (PRR), recombination, and telomere integrity. EXO1 functions in the MMS2 error-free branch of the PRR pathway in the maintenance and repair of stalled replication forks. Studies also suggest that EXO1 plays both structural and catalytic roles during MMR-mediated mutation avoidance. Members of this subgroup include the H3TH (helix-3-turn-helix) domains of EXO1 and other similar eukaryotic 5' nucleases. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. These nucleases have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (Mg2+ or Mn2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases. EXO1 nucleases also have C-terminal Mlh1- and Msh2-binding domains which allow interaction with MMR and PRR proteins, respectively.


Pssm-ID: 188628 [Multi-domain]  Cd Length: 73  Bit Score: 125.77  E-value: 1.65e-34
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLANnpDIVKVIKKIGHYLKMniTVPEDYITGFIRANNTFLYQLVFD 289
Cdd:cd09908     1 EKFRHMCILSGCDYLPSLPGIGLKKAYKLVRRHR--TIEKVIKALRFDGKK--EVPPDYEEGFQKALLTFLHQRVFD 73
PTZ00217 PTZ00217
flap endonuclease-1; Provisional
 1-295 1.92e-34

flap endonuclease-1; Provisional


Pssm-ID: 240317 [Multi-domain]  Cd Length: 393  Bit Score: 136.29  E-value: 1.92e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   1 MGIQGLLQFIKEASePVNVKK-----YKGQAVAVDTYCWLHKGAIACAEKL-------AKGEPTDRYVGFcMKFVNMLLS 68
Cdd:PTZ00217    1 MGIKGLSKFLADKA-PNAIKEqelknYFGRVIAIDASMALYQFLIAIRDDSqggnltnEAGEVTSHISGL-FNRTIRLLE 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  69 YGVKPILVFDGcTLPSKKeversrrerrQSNLLKGKQL-----------LREGKVSEARECFTRSVNITHAMAHKVIKAA 137
Cdd:PTZ00217   79 AGIKPVYVFDG-KPPELK----------SGELEKRRERreeaeeelekaIEEGDDEEIKKQSKRTVRVTKEQNEDAKKLL 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 138 RALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGNG----LEVDQARlgMCKQLGdvFTEE 213
Cdd:PTZ00217  148 RLMGIPVIEAPCEAEAQCAELVKKGKVYAVATEDMDALTFGTPVLLRNLNFSEAKkrpiQEINLST--VLEELG--LSMD 223

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 214 KFRYMCILSGCDYLASLRGIGLAKACKVLRLANNpdIVKVIKKIGhylKMNITVPEDYitGFIRANNTFLYQLVFDPIQR 293
Cdd:PTZ00217  224 QFIDLCILCGCDYCDTIKGIGPKTAYKLIKKYKS--IEEILEHLD---KTKYPVPENF--DYKEARELFLNPEVTPAEEI 296


                  ..
gi 1958672601 294 KL 295
Cdd:PTZ00217  297 DL 298
XPGN smart00485
Xeroderma pigmentosum G N-region; domain in nucleases
 1-86 1.07e-30

Xeroderma pigmentosum G N-region; domain in nucleases


Pssm-ID: 214690 [Multi-domain]  Cd Length: 99  Bit Score: 116.18  E-value: 1.07e-30
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601    1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDGC 80
Cdd:smart00485   1 MGIKGLWPLLKPVVREVPLEALRGKTLAIDASIWLYQFLTACREKLGTPLPNSKHLMGLFYRTCRLLEFGIKPIFVFDGK 80


                   ....*.
gi 1958672601   81 TLPSKK 86
Cdd:smart00485  81 PPPLKS 86
XPG_I pfam00867
XPG I-region;
141-225 1.51e-29

XPG I-region;


Pssm-ID: 459970 [Multi-domain]  Cd Length: 87  Bit Score: 112.22  E-value: 1.51e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 141 GVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGNG---LEVDQARLG-MCKQLGdvFTEEKFR 216
Cdd:pfam00867   1 GIPYVVAPGEAEAQCAYLQKSGLVDAVISEDSDVLLFGAPRLLRNLTGKSKKkskVPVEEIDLEkILKELG--LTREQLI 78


                  ....*....
gi 1958672601 217 YMCILSGCD 225
Cdd:pfam00867  79 DLAILLGCD 87
H3TH_FEN1-like cd09901
H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases: FEN1 ...
 213-289 1.48e-28

H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases: FEN1 (eukaryotic) and EXO1; The 5' nucleases within this family are capable of both 5'-3' exonucleolytic activity and cleaving bifurcated or branched DNA, in an endonucleolytic, structure-specific manner, and are involved in DNA replication, repair, and recombination. This family includes the H3TH (helix-3-turn-helix) domains of eukaryotic Flap Endonuclease-1 (FEN1), Exonuclease-1 (EXO1), and other eukaryotic homologs. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. The nucleases within this family have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (i. e., Mg2+, Mn2+, Zn2+, or Co2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases.


Pssm-ID: 188621 [Multi-domain]  Cd Length: 73  Bit Score: 108.77  E-value: 1.48e-28
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLANnpDIVKVIKKIGHYLKMniTVPEDYITGFIRANNTFLYQLVFD 289
Cdd:cd09901     1 EQFIDLCILSGCDYLPSIPGIGPKTAYKLIKKHK--SIEKVLKALRSNKKK--KVPVPYEEPFKEARLTFLHQRVYD 73
PIN_XPG_RAD2 cd09868
FEN-like PIN domains of Xeroderma pigmentosum complementation group G (XPG) nuclease, a ...
 2-183 1.96e-27

FEN-like PIN domains of Xeroderma pigmentosum complementation group G (XPG) nuclease, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; The Xeroderma pigmentosum complementation group G (XPG) nuclease plays a central role in nucleotide excision repair (NER) in cleaving DNA bubble structures or loops. XPG is a member of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350216 [Multi-domain]  Cd Length: 209  Bit Score: 110.68  E-value: 1.96e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   2 GIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKgAIAcAEKLAKGEPTDR--YVGF---CMKfvnmLLSYGVKPILV 76
Cdd:cd09868     1 GVKGLWKLLEPTGRPVSLESLEGKVLAVDASIWLHQ-FVK-GMRDNEGNSVPNahLLGFfrrICK----LLFYGIKPVFV 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  77 FDGCTlPSKKeversrrerrqsnllkgKQLLREGKvsearecftrsvNITHAMAHKVIKAARALGVDCLVAPYEADAQLA 156
Cdd:cd09868    75 FDGPA-PALK-----------------RRTLARRR------------SVTDEMYEEIQELLRLFGIPYIVAPMEAEAQCA 124

                         170       180
                  ....*....|....*....|....*..
gi 1958672601 157 YLNKAGIVQAVITEDSDLLAFGCKKVI 183
Cdd:cd09868   125 FLERLGLVDGVITDDSDVFLFGAKRVY 151
PIN_YEN1 cd09870
FEN-like PIN domains of Saccharomyces cerevisiae endonuclease 1 (YEN1), Chaetomium ...
 2-183 2.25e-24

FEN-like PIN domains of Saccharomyces cerevisiae endonuclease 1 (YEN1), Chaetomium thermophilum junction-resolving enzyme GEN1, and fungal homologs; Fungal Endonuclease 1 (YEN1 and GEN1, GEN1 is known as YEN1 in Saccharomyces cerevisiae) is a four-way (Holliday) junction resolvase. Members of this subgroup belong to the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350218 [Multi-domain]  Cd Length: 229  Bit Score: 102.35  E-value: 2.25e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   2 GIQGLLQFIKEASEPV---------NVKKYKGQA--VAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVnMLLSYG 70
Cdd:cd09870     1 GIPGLWDLLEPAAESRslaelavveEFNKRGGRPlrIGIDASIWLFHAQSSFGGGHIQAGENPELRTLFYRLA-RLLSLP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  71 VKPILVFDGCTLPSKKeversrrerrqsnllKGKQllregkvsearecftRSVNITHAMAHKVIKAARALGVDCLVAPYE 150
Cdd:cd09870    80 IQPVFVFDGPNRPPFK---------------RGKK---------------VGKSTPHWLTKLFKELLDAFGFPWHEAPGE 129

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1958672601 151 ADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVI 183
Cdd:cd09870   130 AEAELARLQRLGVVDAVLTDDSDALVFGATTVL 162
XPGI smart00484
Xeroderma pigmentosum G I-region; domain in nucleases
 138-207 1.22e-23

Xeroderma pigmentosum G I-region; domain in nucleases


Pssm-ID: 214689 [Multi-domain]  Cd Length: 73  Bit Score: 94.96  E-value: 1.22e-23
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1958672601  138 RALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVILKMDQFGN-GLEVDQARL-GMCKQLG 207
Cdd:smart00484   1 RLMGIPYIVAPYEAEAQCAYLAKSGLVDAIITEDSDLLLFGAPRLYRNLFFSGKkKLEFRIIDLeSVLKELG 72
PIN_FEN1-like cd09856
FEN-like PIN domains of Flap endonuclease-1 (FEN1)-like, structure-specific, ...
 5-183 1.69e-23

FEN-like PIN domains of Flap endonuclease-1 (FEN1)-like, structure-specific, divalent-metal-ion dependent, 5' nucleases; PIN (PilT N terminus) domain of Flap endonuclease-1 (FEN1)-like nucleases: FEN1, Gap endonuclease 1 (GEN1) and Xeroderma pigmentosum complementation group G (XPG) nuclease. Nucleases in this subfamily are members of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350206 [Multi-domain]  Cd Length: 235  Bit Score: 99.92  E-value: 1.69e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   5 GLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNmLLSYGVKPILVFDGCTLPS 84
Cdd:cd09856     1 GFWKIIGPSKRRISLESLRGKRVAIDASIWIYQFLTAVRGQGGNGVSNSHIRGLFYRIIR-LLENGIKPVFVFDGEPPKL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  85 KKEVERSRRERRQSNLLKGKQLLREGKVSEARECFTRSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNKAGIV 164
Cdd:cd09856    80 KKRTRRKRKERRQGAEESAKSAVEDELFEEQSKDKKRSGTVTKVMTAECKHLLSLFGIPYVDAPGEAEAQCAYLEQQGIV 159

                         170
                  ....*....|....*....
gi 1958672601 165 QAVITEDSDLLAFGCKKVI 183
Cdd:cd09856   160 DAVLTEDVDTFLFGSPVVY 178
H3TH_FEN1-XPG-like cd09897
H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases; The 5' ...
 213-284 1.67e-16

H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases; The 5' nucleases within this family are capable of both 5'-3' exonucleolytic activity and cleaving bifurcated or branched DNA, in an endonucleolytic, structure-specific manner, and are involved in DNA replication, repair, and recombination. This family includes the H3TH (helix-3-turn-helix) domains of Flap Endonuclease-1 (FEN1), Exonuclease-1 (EXO1), Mkt1, Gap Endonuclease 1 (GEN1), Xeroderma pigmentosum complementation group G (XPG) nuclease, and other eukaryotic and archaeal homologs. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. With the except of the Mkt1-like proteins, the nucleases within this family have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (i. e., Mg2+, Mn2+, Zn2+, or Co2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases.


Pssm-ID: 188617 [Multi-domain]  Cd Length: 68  Bit Score: 74.56  E-value: 1.67e-16
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLAnnPDIVKVIKKIGHYLKMNitVPEDYITGFIRANNTFLY 284
Cdd:cd09897     1 EQFIDLCILSGCDYLPGLPGIGPKTALKLIKEY--GSLEKVLKALRDDKKDK--VPVPYDFPYKKARELFLH 68
PIN_FEN1 cd09867
FEN-like PIN domains of Flap endonuclease-1, a structure-specific, divalent-metal-ion ...
 15-183 1.94e-16

FEN-like PIN domains of Flap endonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; Flap endonuclease-1 (FEN1) is involved in multiple DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity) and DNA repair processes (long-patch base excision repair) in eukaryotes and archaea. Interaction between FEN1 and PCNA (Proliferating cell nuclear antigen) is an essential prerequisite to FEN1's DNA replication functionality and stimulates FEN1 nuclease activity by 10-50 fold. FEN1 belongs to the FEN1-EXO1-like subfamily of structure-specific, 5' nucleases (FEN-like family). Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. FEN1 has a C-terminal extension containing residues forming the consensus PIP-box - Qxx(M/L/I)xxF(Y/F) which serves to anchor FEN1 to PCNA.


Pssm-ID: 350215 [Multi-domain]  Cd Length: 251  Bit Score: 79.75  E-value: 1.94e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  15 EPVNVKKYKGQAVAVDTYCWLHKGAIAC----AEKL--AKGEPTDRYVGFCMKFVNMLlSYGVKPILVFDGctLPSKkev 88
Cdd:cd09867    10 KEIELKDLSGKKIAIDASNALYQFLSAIrqpdGTPLtdSKGRVTSHLSGLFYRTINLL-ENGIKPVYVFDG--KPPE--- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  89 ersrrerrqsnlLKGKQL-----------------LREGKVSEARECFTRSVNITHAMAHKVIKAARALGVDCLVAPYEA 151
Cdd:cd09867    84 ------------LKSGELekrrerreeaeekleeaLEEGDLEEARKYAKRTVRVTKEMVEEAKKLLDLMGIPYVQAPSEG 151

                         170       180       190
                  ....*....|....*....|....*....|..
gi 1958672601 152 DAQLAYLNKAGIVQAVITEDSDLLAFGCKKVI 183
Cdd:cd09867   152 EAQAAYLVKKGDVYAVASQDYDSLLFGAPRLV 183
PRK03980 PRK03980
flap endonuclease-1; Provisional
 47-259 2.88e-16

flap endonuclease-1; Provisional


Pssm-ID: 235185 [Multi-domain]  Cd Length: 292  Bit Score: 80.25  E-value: 2.88e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  47 AKGEPTDRYVGFCMKFVNmLLSYGVKPILVFDGCTLPSKKEVERSRRERRQSNLLKGKQLLREGKVSEARECFTRSVNIT 126
Cdd:PRK03980    3 SKGRITSHLSGIFYRTIN-LLENGIKPVYVFDGKPPELKAEEIEERREVREEAEEKYEEAKEEGDLEEARKYAQRSSRLT 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601 127 HAMAHKVIKAARALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLAFGCKKVI--------LKMDQFGNGLEVD-- 196
Cdd:PRK03980   82 DEIVEDSKKLLDLMGIPYVQAPSEGEAQAAYMAKKGDAWAVGSQDYDSLLFGAPRLVrnltisgkRKLPGKNVYVEVKpe 161

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 197 ----QARLgmcKQLGdvFTEEKFRYMCILSGCDYLASLRGIGLAKACKVLRlaNNPDIVKVIKKIGH 259
Cdd:PRK03980  162 lielEEVL---KELG--ITREQLIDIAILVGTDYNPGIKGIGPKTALKLIK--KHGDLEKVLEERGF 221
XPG_N pfam00752
XPG N-terminal domain;
2-86 3.57e-16

XPG N-terminal domain;


Pssm-ID: 395609 [Multi-domain]  Cd Length: 100  Bit Score: 74.70  E-value: 3.57e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   2 GIQGLLQFIKE--ASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSYGVKPILVFDG 79
Cdd:pfam00752   1 GIKGLLPILKPvaLIRPVDIEALEGKTLAIDASIWLYQFLKAVRDQLGNALQNTSHLMGFFSRLCRLKDFGIKPIFVFDG 80


                  ....*..
gi 1958672601  80 CTLPSKK 86
Cdd:pfam00752  81 GPPPLKA 87
PIN_MKT1 cd09858
FEN-like PIN domains of Mkt1, a global regulator of mRNAs encoding mitochondrial proteins and ...
 61-187 3.83e-16

FEN-like PIN domains of Mkt1, a global regulator of mRNAs encoding mitochondrial proteins and eukaryotic homologs; The Mkt1 gene product interacts with the Poly(A)-binding protein associated factor, Pbp1, and is present at the 3' end of RNA transcripts during translation. The Mkt1-Pbp1 complex is involved in the post-transcriptional regulation of HO endonuclease expression. Mkt1 and eukaryotic homologs are atypical members of the structure-specific, 5' nuclease family (FEN-like). Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. Although Mkt1 appears to possess both a PIN and H3TH domain, the Mkt1 PIN domain lacks several of the active site residues necessary to bind essential divalent metal ion cofactors (Mg2+/Mn2+) required for nuclease activity in this family. Also, Mkt1 lacks the glycine-rich loop in the H3TH domain which is proposed to facilitate duplex DNA binding.


Pssm-ID: 350208 [Multi-domain]  Cd Length: 206  Bit Score: 77.96  E-value: 3.83e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  61 KFVNMLLSYGVKPILVFDGCTLPSKKEVERSRRERRQSnLLKGKQLLREGKVSEARECFTRSVNI-THAMAHKVIKAARA 139
Cdd:cd09858    64 SDLEQLKKLNITPVFVFNGLSLKGQDEPSSQSEQAAQK-REEAWDLYEKGQADQAVKAFGESGSYrLEDLYRLLQRILKE 142

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1958672601 140 LGVDCLVAPYEADAQLAYL--NKAGIVQAVITeDSDLLAFGCKKVILKMD 187
Cdd:cd09858   143 RGVEFLVAPYSAWAQLAYLekHGKQYIDAIYG-STELLLFGVDKVITSID 191
PIN_GEN1 cd09869
FEN-like PIN domains of Gap Endonuclease 1, a structure-specific, divalent-metal-ion dependent, ...
 2-188 5.07e-15

FEN-like PIN domains of Gap Endonuclease 1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs; Gap Endonuclease 1 (GEN1) is a Holliday junction resolvase reported to symmetrically cleave Holliday junctions and allow religation without additional processing. GEN1 is a member of the structure-specific, 5' nuclease family (FEN-like) that catalyzes hydrolysis of DNA duplex-containing nucleic acid structures during DNA replication, repair, and recombination. Canonical members of the FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350217 [Multi-domain]  Cd Length: 227  Bit Score: 74.95  E-value: 5.07e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   2 GIQGLLQfikeASEPVNVKKY----KGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFcmKFVNmLLSYGVKPILVF 77
Cdd:cd09869     1 GVKGLWT----ILDPVKKRKPlselRGKTLAVDLSIWICEAQTVLALFETVPKPHLRNLFF--RTVN-LLRLGIKPVFVL 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  78 DGCTlPSKKEVERSRRERRQSNLLKGKQLLREGKVSEarecFTRSVNITHAMAhkvikaaRALGVDCLVAPYEADAQLAY 157
Cdd:cd09869    74 DGDA-PELKLQTIKKRNAARFGGAKKKGGSKKRGRSR----FSRVLKECEELL-------ELLGVPVVQAPGEAEALCAL 141

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1958672601 158 LNKAGIVQAVITEDSDLLAFGCKKVI--LKMDQ 188
Cdd:cd09869   142 LNAEGLVDGCITNDGDAFLYGARTVYrnFSLNT 174
H3TH_FEN1-Euk cd09907
H3TH domain of Flap Endonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' ...
 213-289 2.33e-09

H3TH domain of Flap Endonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease: Eukaryotic homologs; Members of this subgroup include the H3TH (helix-3-turn-helix) domains of eukaryotic Flap endonuclease-1 (FEN1), 5' nucleases. FEN1 is involved in multiple DNA metabolic pathways, including DNA replication processes (5' flap DNA endonuclease activity and double stranded DNA 5'-exonuclease activity) and DNA repair processes (long-patch base excision repair) in eukaryotes and archaea. Interaction between FEN1 and PCNA (Proliferating cell nuclear antigen) is an essential prerequisite to FEN1's DNA replication functionality and stimulates FEN1 nuclease activity by 10-50 fold. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. The nucleases within this subfamily have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (Mg2+ or Mn2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases. Also, FEN1 has a C-terminal extension containing residues forming the consensus PIP-box - Qxx(M/L/I)xxF(Y/F) which serves to anchor FEN1 to PCNA.


Pssm-ID: 188627 [Multi-domain]  Cd Length: 70  Bit Score: 54.09  E-value: 2.33e-09
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVLRLANNpdIVKVIKKIGhylKMNITVPEDYItgFIRANNTFLYQLVFD 289
Cdd:cd09907     1 EQFIDLCILLGCDYCESIKGIGPKTALKLIKKHKS--IEKILENID---KSKYPVPEDWP--YKEARELFLNPEVTD 70
rad2 TIGR00600
DNA excision repair protein (rad2); All proteins in this family for which functions are known ...
 97-242 4.31e-07

DNA excision repair protein (rad2); All proteins in this family for which functions are known are flap endonucleases that generate the 3' incision next to DNA damage as part of nucleotide excision repair. This family is related to many other flap endonuclease families including the fen1 family. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273166 [Multi-domain]  Cd Length: 1034  Bit Score: 53.75  E-value: 4.31e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601   97 QSNLLKGKQLLREGKVSEAREcftrSVNITHAMAHKVIKAARALGVDCLVAPYEADAQLAYLNKAGIVQAVITEDSDLLA 176
Cdd:TIGR00600  745 EANLLAEQNSLKAQKQQQKRI----AAEVTGQMILESQELLRLFGIPYIVAPMEAEAQCAILDLLDQTSGTITDDSDIWL 820

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958672601  177 FGCKKVILKMDQFGNGLEVDQArLGMCKQLGdvFTEEKFRYMCILSGCDYLASLRGIGLAKACKVL 242
Cdd:TIGR00600  821 FGARHVYKNFFNQNKFVEYYQY-VDIHNQLG--LDRNKLINLAYLLGSDYTEGIPTVGPVSAMEIL 883
PIN_FEN-like cd09853
FEN-like PIN domains of structure-specific 5' nucleases (or Flap endonuclease-1-like) involved ...
 66-186 5.05e-07

FEN-like PIN domains of structure-specific 5' nucleases (or Flap endonuclease-1-like) involved in DNA replication, repair, and recombination; Structure-specific 5' nucleases are capable of both 5'-3' exonucleolytic activity and cleaving bifurcated or branched DNA, in an endonucleolytic, structure-specific manner. The family includes the PIN (PilT N terminus) domains of Flap endonuclease-1 (FEN1), exonuclease-1 (EXO1), Mkt1, Gap Endonuclease 1 (GEN1), and Xeroderma pigmentosum complementation group G (XPG) nuclease. Also included are the PIN domains of the 5'-3' exonucleases of DNA polymerase I and single domain protein homologs, as well as, the bacteriophage T4- and T5-5' nucleases, and other homologs. Canonical members of this FEN-like family possess a PIN domain with a two-helical structure insert (also known as the helical arch, helical clamp or I domain) of variable length (approximately 16 to 800 residues), and at the C-terminus of the PIN domain a H3TH (helix-3-turn-helix) domain, an atypical helix-hairpin-helix-2-like region. Both the H3TH domain (not included in this model) and the helical arch/clamp region are involved in DNA binding. The PIN domain belongs to a large nuclease superfamily. The structural properties of the PIN domain indicate its putative active center, consisting of invariant acidic amino acid residues (putative metal-binding residues), is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.


Pssm-ID: 350204 [Multi-domain]  Cd Length: 174  Bit Score: 50.56  E-value: 5.05e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958672601  66 LLSYGVKPILVFDGCTLPSKKEVERSRRERRQSNLLKGKQLLREGKvsearECFTRSVNITHAMAHKVIKAARA-LGVDC 144
Cdd:cd09853    39 KLKPGIKPILLFDGGKPKAKKGNRDKRRERRAREEDRKKGQLKEHK-----EFDKRLIELGPEYLIRLFELLKHfMGIPV 113

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1958672601 145 LVAPYEADAQLAYLNKA----GIVQAVITEDSDLLAFG--CKKVILKM 186
Cdd:cd09853   114 MDAPGEAEDEIAYLVKKhkhlGTVHLIISTDGDFLLLGtdHPYIPRNL 161
H3TH_StructSpec-5'-nucleases cd00080
H3TH domains of structure-specific 5' nucleases (or flap endonuclease-1-like) involved in DNA ...
 213-278 1.44e-06

H3TH domains of structure-specific 5' nucleases (or flap endonuclease-1-like) involved in DNA replication, repair, and recombination; The 5' nucleases of this superfamily are capable of both 5'-3' exonucleolytic activity and cleaving bifurcated or branched DNA, in an endonucleolytic, structure-specific manner, and are involved in DNA replication, repair, and recombination. The superfamily includes the H3TH (helix-3-turn-helix) domains of Flap Endonuclease-1 (FEN1), Exonuclease-1 (EXO1), Mkt1, Gap Endonuclease 1 (GEN1) and Xeroderma pigmentosum complementation group G (XPG) nuclease. Also included are the H3TH domains of the 5'-3' exonucleases of DNA polymerase I and single domain protein homologs, as well as, the bacteriophage T4 RNase H, T5-5'nuclease, and other homologs. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the C-terminal region of the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. Typically, the nucleases within this superfamily have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (i. e., Mg2+, Mn2+, Zn2+, or Co2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one or two Asp residues from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases.


Pssm-ID: 188616 [Multi-domain]  Cd Length: 71  Bit Score: 46.60  E-value: 1.44e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1958672601 213 EKFRYMCILSGCDYL--ASLRGIG----LAKACKVLRLANNPDIVKVIKKIghyLKMNITVPEDYITGFIRA 278
Cdd:cd00080     1 EQFIDLCALVGCDYSdnPGVPGIGpktaAKLALKYGSLEGILENLDELKGK---KREKLEEPKEYAFLSRKL 69
HhH2 smart00279
Helix-hairpin-helix class 2 (Pol1 family) motifs;
213-243 8.06e-05

Helix-hairpin-helix class 2 (Pol1 family) motifs;


Pssm-ID: 197623 [Multi-domain]  Cd Length: 36  Bit Score: 40.51  E-value: 8.06e-05
                           10        20        30
                   ....*....|....*....|....*....|...
gi 1958672601  213 EKFRYMCILSG--CDYLASLRGIGLAKACKVLR 243
Cdd:smart00279   2 EQFIDYAILVGdySDNIPGVKGIGPKTALKLLR 34
H3TH_XPG-like cd09900
H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases: FEN1 ...
 213-262 2.65e-04

H3TH domains of Flap endonuclease-1 (FEN1)-like structure specific 5' nucleases: FEN1 (archaeal), GEN1, YEN1, and XPG; The 5' nucleases within this family are capable of both 5'-3' exonucleolytic activity and cleaving bifurcated or branched DNA, in an endonucleolytic, structure-specific manner, and are involved in DNA replication, repair, and recombination. This family includes the H3TH (helix-3-turn-helix) domains of archaeal Flap Endonuclease-1 (FEN1), Gap Endonuclease 1 (GEN1), Yeast Endonuclease 1 (YEN1), Xeroderma pigmentosum complementation group G (XPG) nuclease, and other eukaryotic and archaeal homologs. These nucleases contain a PIN (PilT N terminus) domain with a helical arch/clamp region/I domain (not included here) and inserted within the PIN domain is an atypical helix-hairpin-helix-2 (HhH2)-like region. This atypical HhH2 region, the H3TH domain, has an extended loop with at least three turns between the first two helices, and only three of the four helices appear to be conserved. Both the H3TH domain and the helical arch/clamp region are involved in DNA binding. Studies suggest that a glycine-rich loop in the H3TH domain contacts the phosphate backbone of the template strand in the downstream DNA duplex. With the except of the Mkt1-like proteins, the nucleases within this family have a carboxylate rich active site that is involved in binding essential divalent metal ion cofactors (i. e., Mg2+, Mn2+, Zn2+, or Co2+) required for nuclease activity. The first metal binding site is composed entirely of Asp/Glu residues from the PIN domain, whereas, the second metal binding site is composed generally of two Asp residues from the PIN domain and one Asp residue from the H3TH domain. Together with the helical arch and network of amino acids interacting with metal binding ions, the H3TH region defines a positively charged active-site DNA-binding groove in structure-specific 5' nucleases.


Pssm-ID: 188620 [Multi-domain]  Cd Length: 52  Bit Score: 39.39  E-value: 2.65e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1958672601 213 EKFRYMCILSGCDYLASLRGIGLAKACKVL--------RLANNPDIvkvikkIGHYLK 262
Cdd:cd09900     1 EQLILLALLLGTDYNPGVPGIGPKTALELLkefgedleKFLESEEI------LEAYLN 52
rad2 TIGR00600
DNA excision repair protein (rad2); All proteins in this family for which functions are known ...
 1-79 9.80e-04

DNA excision repair protein (rad2); All proteins in this family for which functions are known are flap endonucleases that generate the 3' incision next to DNA damage as part of nucleotide excision repair. This family is related to many other flap endonuclease families including the fen1 family. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273166 [Multi-domain]  Cd Length: 1034  Bit Score: 42.96  E-value: 9.80e-04
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1958672601    1 MGIQGLLQFIKEASEPVNVKKYKGQAVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNmLLSYGVKPILVFDG 79
Cdd:TIGR00600    1 MGVQGLWKLLECSGRPVSPETLEGKRLAVDISIWLNQALKGVRDREGNAIKNSHLLTLFHRLCK-LLFFRIRPIFVFDG 78
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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