Solute carrier family 22 members 4 and 5 (also called Organic cation/carnitine transporters 1 and 2) of the Major Facilitator Superfamily of transporters; This subfamily is composed of solute carrier family 22 members 4 (SLC22A4) and 5 (SLC22A5), and similar proteins. SLC22A4 is also called ergothioneine transporter (ETT) or organic cation/carnitine transporter 1 (OCTN1). It is a sodium-ion dependent, low affinity carnitine transporter, and a highly specific transporter for the uptake of ergothioneine (ET), a thiolated derivative of histidine with antioxidant properties. ET is a natural compound produced only by certain fungi and bacteria and must be absorbed from the diet by humans and other vetebrates. SLC22A5, also called organic cation/carnitine transporter 2 (OCTN2), is a sodium-ion dependent, high affinity carnitine transporter involved in the active cellular uptake of carnitine. SLC22A4/5 belongs to the Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   1 MVLPLFAYFIRDWRMLLLALTVPGVLCGALWWFIPesprwlisqgrvkeaeviirkaakfngivapSTIFdpselqdlns 80
Cdd:cd17376   139 MLLPLFAYFIRDWRMLLLALTLPGVLCVPLWWFIP-------------------------------ITTI---------- 177

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  81 kkpqshhiydlvrtrniriitiMSIILWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSI 160
Cdd:cd17376   178 ----------------------MSLLLWMAISVGYFGLSLNTPNLHGDPYLNCFLSAVIEVPAYIIAWLLLRSLPRRYSI 235

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 161 SAALFLGGSVLLFIQLVPSELFYLSTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYL 240
Cdd:cd17376   236 AATLVLGGGVLLFIQLVPTDLQVLATALVMLGKFGITAAFSMVYVYTAELYPTVVRNMGVGVCSTASRIGSIISPYFVYL 315

                         250       260
                  ....*....|....*....|....*..
gi 1958657956 241 GAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:cd17376   316 GAYDRYLPYILMGSLTVLIGILTLFLP 342

Sugar phosphate permease [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 134 FLLAAVEVPAYVLAWLLLQHLPRRYSISAALFLGGSVLLFIQLVPSELFYLSTALVMVGKFGITSAYSMVYVYTAELYPT 213
Cdd:COG2271   223 SLPFLAGIVGSLLGGWLSDRLGRRRKLVLAIGLLLAALALLLLALLPSPALAIALLFLAGFGLGGAFGLLWALAAELFPK 302

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1958657956 214 VVRNMGVGVSSTASRLGSILSPYFV--YLGAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:COG2271   303 KARGTASGLVNTFGFLGGALGPLLVgyLLDATGYQAAFLLLAALALLAALLALLLL 358

Solute carrier family 22 members 4 and 5 (also called Organic cation/carnitine transporters 1 and 2) of the Major Facilitator Superfamily of transporters; This subfamily is composed of solute carrier family 22 members 4 (SLC22A4) and 5 (SLC22A5), and similar proteins. SLC22A4 is also called ergothioneine transporter (ETT) or organic cation/carnitine transporter 1 (OCTN1). It is a sodium-ion dependent, low affinity carnitine transporter, and a highly specific transporter for the uptake of ergothioneine (ET), a thiolated derivative of histidine with antioxidant properties. ET is a natural compound produced only by certain fungi and bacteria and must be absorbed from the diet by humans and other vetebrates. SLC22A5, also called organic cation/carnitine transporter 2 (OCTN2), is a sodium-ion dependent, high affinity carnitine transporter involved in the active cellular uptake of carnitine. SLC22A4/5 belongs to the Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   1 MVLPLFAYFIRDWRMLLLALTVPGVLCGALWWFIPesprwlisqgrvkeaeviirkaakfngivapSTIFdpselqdlns 80
Cdd:cd17376   139 MLLPLFAYFIRDWRMLLLALTLPGVLCVPLWWFIP-------------------------------ITTI---------- 177

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  81 kkpqshhiydlvrtrniriitiMSIILWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSI 160
Cdd:cd17376   178 ----------------------MSLLLWMAISVGYFGLSLNTPNLHGDPYLNCFLSAVIEVPAYIIAWLLLRSLPRRYSI 235

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 161 SAALFLGGSVLLFIQLVPSELFYLSTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYL 240
Cdd:cd17376   236 AATLVLGGGVLLFIQLVPTDLQVLATALVMLGKFGITAAFSMVYVYTAELYPTVVRNMGVGVCSTASRIGSIISPYFVYL 315

                         250       260
                  ....*....|....*....|....*..
gi 1958657956 241 GAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:cd17376   316 GAYDRYLPYILMGSLTVLIGILTLFLP 342

Organic anion transporters of the Major Facilitator Superfamily of transporters; Organic anion transporters (OATs) generally display broad substrate specificity and they facilitate the exchange of extracellular with intracellular organic anions (OAs). Several OATs have been characterized including OAT1-10 and urate anion exchanger 1 (URAT1, also called SLC22A12). Many OATs occur in renal proximal tubules, the site of active drug secretion. OATs mediate the absorption, distribution, and excretion of a diverse array of environmental toxins, and clinically important drugs, including anti-HIV therapeutics, anti-tumor drugs, antibiotics, anti-hypertensives, and anti-inflammatories, and therefore is critical for the survival of the mammalian species. OAT falls into the SLC22 (solute carrier 22) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   1 MVLPLFAYFIRDWRMLLLALTVPGVLCGALWWFIPEspRWLISqgrvkeaeviirkaakfngivapstifdpselqdlns 80
Cdd:cd17374   138 MLLAGLAYAIRDWRLLQLVVSVPFFLFFLYSWWLPK--RTLIL------------------------------------- 178

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  81 kkpqshhiydlvrtrniriitimsIILWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSI 160
Cdd:cd17374   179 ------------------------SFVWFANSFSYYGLSLDLQHLGLNVFLLQFLFGAVEVPARCLALFLLNHMGRRISQ 234

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 161 SAALFLGGSVLLFIQLVPSELFYLSTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYL 240
Cdd:cd17374   235 AVLLLLAGLCILANIFVPQEMQTLRTVLAVLGKGSSAAAFTISYVYTAELFPTVLRQTGMGLNSMFARIGGILAPLIMLL 314

                         250       260
                  ....*....|....*....|....*..
gi 1958657956 241 GAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:cd17374   315 GVYSPLLPMIIYGVLPILAGLLALLLP 341

Solute carrier family 22 member 15 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 15 (SLC22A15) is also called fly-like putative transporter 1 (FLIPT1). It is expressed at the highest levels in the kidney and brain. It is a member of the SLC22 family of transporters, which includes organic cation transporters (OCTs), organic zwitterion/cation transporters (OCTNs), and organic anion transporters (OATs). SLC22 transporters interact with a variety of compounds that include drugs of abuse, environmental toxins, opioid analgesics, antidepressant and anxiolytic agents, and neurotransmitters and their metabolites. SLC22A15 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 107 LWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQH--LPRRYSISAALFLGGSVLLFIQLVP----SE 180
Cdd:cd17377   185 VWFVCSLVYYGLTLNAGDLGGNIYLNLALSGLVELPAYPLCIYLINQkwSGRRRTLAGFLFLGGIACLLVMFVPekkdSG 264

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 181 LFYL--STALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYLGAYDRFLPYILMGSLTIL 258
Cdd:cd17377   265 VFAVvnSTSLSLLGKLTISAAFNIVYIYTSELYPTVVRNAGLGVCSMFSRVGGIIAPFIPSLKSVQWSLPFIVFGASGLS 344

                  ....*....
gi 1958657956 259 TAILTLFFP 267
Cdd:cd17377   345 AGLLSLLLP 353

Solute carrier family 22 member 7 (also called Organic anion transporter 2) of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 7 (SLC22A7), also called organic anion transporter 2 (OAT2) mediates sodium-independent transport of a variety of organic anions including prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate. It also plays a role in renal uric acid uptake from blood as a first step of tubular secretion. OAT2 belongs to the Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major Facilitator Superfamily (MFS)of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   1 MVLPLFAYFIRDWRMLLLALTVPGVLCGALWWFIPesprwlisqgrvkeaeviirKAAKFNGIVapstifdpselqdlns 80
Cdd:cd17447   138 MLLALVAYLIRDWRWLILAVTLPCAPGIITWWWVP--------------------KISLCTGIV---------------- 181

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  81 kkpqshhiydlvrtrniriitimsiilWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSI 160
Cdd:cd17447   182 ---------------------------WFGVAFTYYGISFNISGFGLNVYLTQFIYGAIELPAKLLAYLLLDRIGRRLTQ 234

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 161 SAALFLGGSVLLFIQLVPSELFYLSTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYL 240
Cdd:cd17447   235 AGSLILTGLSIGINILIPSEFTVVRTVIAVLGKGFSEAAFTTVFLYTSELYPTVLRQNGMGYTSFIARLGGSLAPLVILL 314

                         250       260
                  ....*....|....*....|....*..
gi 1958657956 241 GAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:cd17447   315 DDVWLSLPQVIFCGVALLAGGVAFLLP 341

Plant organic cation/carnitine transporters of the Major Facilitator Superfamily of transporters; Plant organic cation/carnitine transporters (OCTs) are sequence-similar to their animal counterparts, which are broad-specificity transporters that play a critical role in the excretion and distribution of endogeneous organic cations and for the uptake, elimination and distribution of cationic drugs, toxins, and environmental waste products. Little is know about plant OCTs. In Arabidopsis, there are six genes belonging to this family that show distinct, organ-specific expression pattern of the individual genes. AtOCT1 has been found to affect root development and carnitine-related responses in Arabidopsis. AtOCT4, 5 and 6 are up-regulated during drought stress, AtOCT3 and 5 during cold stress and AtOCT5 and 6 during salt stress treatments. Plant OCTs belongs to the Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 107 LWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSISAALFLGGSVLLFIQLVPSEL-FYLS 185
Cdd:cd17378   181 IGFGIGMVYYGIPLAVGNLKFNIYLSVALNALSELPAFVITAFLLDRFGRRPSLLGFTLLSGASCLLCFVLSIGGtTSLQ 260

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 186 TALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYLGAYDRFLPYILMGSLTILTAILTLF 265
Cdd:cd17378   261 IALELVGFFGAAGAFNLLYIYTVELFPTCVRNAALGMLRQALVVGGIFAPFIVSLGRKNAALSFGVFGVAILLLGLFALG 340

                  ..
gi 1958657956 266 FP 267
Cdd:cd17378   341 LP 342

MFS transporter, sugar porter (SP) family; This model represent the sugar porter subfamily of the major facilitator superfamily (pfam00083) [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  13 WRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIRK----AAKFNGIVAPSTIFDPSELQDLNSKKPQSHHI 88
Cdd:TIGR00879 195 WRIPLGLQLIPAGLLFLGLFFLPESPRWLVGKGRVEEARKSLARlrgtSGEDKELLDELELIDIKRSIEKRSVQPSWGSL 274

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  89 YDLVRTRNIRIITIMSIILWLTIS----VGYFGL----SLDTPNLHGdiYVNCFLLAAVEVPAYVLAWLLLQHLPRR--- 157
Cdd:TIGR00879 275 FSSTRRIRRRLFLGVVLQWFQQFTginaIMYYSPtifeNAGVSTDHA--FLVSIIVGAVNFAFTFVAIFLVDRFGRRpll 352

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 158 ---YSISAALFLGGSVLLFIQLVPSELF--YLSTALVMVGKFGI-TSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGS 231
Cdd:TIGR00879 353 ligAAGMAICLFVLGILGASFVTGSSKSsgNVAIVFILLFIAFFaMGWGPVPWVIVSEIFPLSLRPKGISIAVAANWLAN 432

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*.
gi 1958657956 232 IL-SPYFVY-LGAYDRFLPYILMGSLTILTAILTLFF-PESFGAPL 274
Cdd:TIGR00879 433 FIvGFLFPTmLESIGVGGVFIFFGGLNVLGLIFVYFFlPETKGRTL 478

Sugar (and other) transporter;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  13 WRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEviiRKAAKFNGIVAPSTIFDPSELQDLNSKKPQSHHIYDLV 92
Cdd:pfam00083 170 WRIPLGLQLVPALLLIIGLLFLPESPRWLVEKGRLEEAR---EVLAKLRGVPDVDRELDEIKDSLEAGQEAEKASWKELF 246

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  93 RTRNIRIITIMSIILWLTI------SVGYFGLSL-DTPNLHGDIYVNcFLLAAVEVPAYVLAWLLLQHLPRRYsisaALF 165
Cdd:pfam00083 247 STKTRRQRLLIGVMLQIFQqltginAIFYYSTTIfENLGLSDSFLVT-IIVGVVNFVFTFIAIFLVDRFGRRP----LLL 321

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 166 LGGSVLLFIQLVPSELFYLSTA-------------LVMVGKFGITSAySMVYVYTAELYPTVVRNMGVGVSSTASRLGSI 232
Cdd:pfam00083 322 LGAAGMAICFVILGIVALLGVSksdwagivaivfiALFIAFFAMGWG-PVPWVIVSELFPLSVRSKAMALATAANWLANF 400

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1958657956 233 LSPYF--VYLGAYDRFLPYILMGSLTILTAILTLFF-PESFGAPLpDTIDQML 282
Cdd:pfam00083 401 LIGFLfpIITDAIGLGYTFFIFAGLLVLFIIFVFFFvPETKGRTL-EEIDELF 452

Synaptic vesicle 2 (SV2)-related protein-like (SVOPL) of the Major Facilitator Superfamily; Synaptic vesicle 2 (SV2)-related protein-like (SVOPL) or SVOP-like protein is a transporter-like protein that shares structural similarity to the solute carrier family 22 (SLC22), a large family of organic cation and anion transporters. It belongs to the Metazoan Synaptic Vesicle Glycoprotein 2 (SV2) and related small molecule transporter family (SV2-like) of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 149 LLLQHLPRRYSISAALFLGGSVLLFIQLVPSELFYlsTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASR 228
Cdd:cd17442   258 LGINFLGRRLSLVITMGLTAIFFLLLNICTSSAGL--TIFLFMLRALVSANFNTVYIYTAEVYPTTMRALGMGTSGSLCR 335

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1958657956 229 LGSILSPYFV-YLGAYDRFLPYILMGSLTILTAILTLFFP 267
Cdd:cd17442   336 IGAMVAPFISqVLMSASVLGALCLFSSVCIVCAISAFTLP 375

Class 1 and Class 2 Glucose transporters (GLUTs) of the Major Facilitator Superfamily; This subfamily includes Class 1 and Class 2 glucose transporters (GLUTs) including Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1, also called glucose transporter type 1 or GLUT1), SLC2A2-5 (GLUT2-5), SLC2A7 (GLUT7), SLC2A9 (GLUT9), SLC2A11 (GLUT11), SLC2A14 (GLUT14), and similar proteins. GLUTs are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUTs 1-5 are the most thoroughly studied and are well-established as glucose and/or fructose transporters in various tissues and cell types. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  13 WRMLLLALTVPGVLCGALWWFIPESPRWL-ISQGRVKEAEVIIRkaaKFNGIVAP-STIFDPSELQDLNSKKPQSHHIYD 90
Cdd:cd17357   172 WPYLLFFPGIPALLQLAALPFFPESPKFLlISKGDEEEAEKSLK---FLRGIEDDvDQELEEIKKESEQMGDQKQVSLMQ 248

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  91 LVRTRNIRIITIMSIILWLTI-----------SVGYF---GLSLDTPnlhgdIYVNcFLLAAVEVPAYVLAWLLLQHLPR 156
Cdd:cd17357   249 LLRDPSLRLPLLLVLVVSASQqfsginaiffySTFIFenaGFSPQLA-----EWAN-LGIGIVNVLSTIVGPFLIEKVGR 322

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 157 R-------YSISAALFLgGSVLLFIQLVPSELFYLSTALVMVgkFGITsaYSMV-----YVYTAELYPTVVRNMGVGVSS 224
Cdd:cd17357   323 RplllislSVCAVALLL-MSVFLFLSEQNSWMSYGCIVAIFL--FIFF--FAIGlgpipWFIGAELFPQAPRSAAQSLGS 397

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*....
gi 1958657956 225 TASRLGSILSPYFVY----LGAYDRFLPYILmgsLTILTAILTLFF-PE 268
Cdd:cd17357   398 SVNWTSNFIVGMAFPplqsIGGGFVFIIFAI---PCALFLLYLYRYlPE 443

Synaptic vesicle 2-related protein (SVOP) of the Major Facilitator Superfamily; Synaptic vesicle 2 (SV2)-related protein (SVOP) is a transporter-like nucleotide binding protein that localizes to neurotransmitter-containing vesicles. Like SV2, SVOP is expressed in all brain regions, with highest levels in cerebellum, hindbrain and pineal gland. Studies with knockout mice suggets that SVOP may perform a subtle function that is not necessary for survival under normal conditions, since mice lacking SVOP are viable, fertile, and phenotypically normal. SVOP shares structural similarity to the solute carrier family 22 (SLC22), a large family of organic cation and anion transporters. This SVOP subfamily belongs to the Metazoan Synaptic Vesicle Glycoprotein 2 (SV2) and related small molecule transporter family (SV2-like) of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 186 TALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFV-YLGAYDRFLPYILMGSLTILTAILTL 264
Cdd:cd17441   290 TVLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAqVMLESSVYLTLSVYSGCCLLAAIASC 369

                  ...
gi 1958657956 265 FFP 267
Cdd:cd17441   370 FLP 372

Glucose transporter (GLUT) types 6 and 8, Class 3 GLUTs, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of glucose transporter type 6 (GLUT6), GLUT8, plant early dehydration-induced gene ERD6-like proteins, and similar insect proteins including facilitated trehalose transporter Tret1-1. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). Insect Tret1-1 is a low-capacity facilitative transporter for trehalose that mediates the transport of trehalose synthesized in the fat body and the incorporation of trehalose into other tissues that require a carbon source. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1958657956   1 MVLPLFAYFIRdWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIRK 56
Cdd:cd17358   144 LLGYVLGSFLP-WRTLALIGAIPPVVFLILLFFIPESPRWLAKKGREEEAEKSLQF 198

Synaptic vesicle glycoprotein 2 of the Major Facilitator Superfamily of transporters; Synaptic vesicle glycoprotein 2 (SV2) is a transporter-like integral membrane glycoprotein, with 12 transmembrane regions, expressed in vertebrates and is localized to synaptic and endocrine secretory vesicles. Three isoforms have been identified, SV2A, SV2B, and SV2C. SV2A and SV2B are widely expressed in the brain, while SV2C is more restricted to evolutionarily older brain. SV2 isoforms have been shown to be critical for the proper function of the central nervous system. SV2 serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. The SV2 family belongs to the Metazoan Synaptic Vesicle Glycoprotein 2 (SV2) and related small molecule transporter family (SV2-like) of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   7 AYFIRDWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIRKAAKFN--GIVAPSTIFDPSELQDLNSkkpq 84
Cdd:cd17363   159 AYQFHSWRVFVIVCALPSVASVVALTFMPESPRFLLEVGKHDEAWMILKQVHDTNmrAKGHPERVFTVSHIKRMNT---- 234

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  85 shhiydlvrtrniriitIMSIILWLTISVGYFGLSLDTP----------------------------------------- 123
Cdd:cd17363   235 -----------------LFLAIVWFTMSFSYYGLTVWFPdmikylqfedslfeecyfedvtstntffenctfintvfynt 297

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 124 ---------------------------NLHGD----IYVNCFLLAAVEVPAYVLAWLLLQHLPRRYSISAALFLGGSVLL 172
Cdd:cd17363   298 dlfeykfidsrflnstfflkekcgchlNDDEEnaylIYLVSFLGSLSVLPGNIVSALLMDKIGRLKMIGGSMLLSAVCCF 377

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 173 FIQLVPSElfylSTALVMVGKFGITS--AYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPY-FVYLGAYDRFLPY 249
Cdd:cd17363   378 FLFFGNSE----SAMIGLQCLFCGVSiaAWNALDVVTVELYPTDKRATAFGFLNGLCKLAAILGNSiFGSFVGITKVIPI 453

                         330       340
                  ....*....|....*....|
gi 1958657956 250 ILMGSLTILTAILTLFFPES 269
Cdd:cd17363   454 LLASAALVCGGLLALKLPET 473

Synaptic vesicle glycoprotein 2C of the Major Facilitator Superfamily of transporters; Synaptic vesicle glycoprotein 2 (SV2) is a transporter-like integral membrane glycoprotein, with 12 transmembrane regions, expressed in vertebrates and is localized to synaptic and endocrine secretory vesicles. Three isoforms have been identified, SV2A, SV2B, and SV2C. SV2A and SV2B are widely expressed in the brain, while SV2C is more restricted to evolutionarily older brain. SV2 isoforms have been shown to be critical for the proper function of the central nervous system. SV2 serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. SV2C exhibits enriched expression in several basal ganglia nuclei, and has been found to be involved in normal operation of the basal ganglia network and could be also be involved in system adaptation in basal ganglia pathological conditions. SV2C belongs to the Metazoan Synaptic Vesicle Glycoprotein 2 (SV2) and related small molecule transporter family (SV2-like) of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   7 AYFIRDWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIRKAAKFNGIV--APSTIFDPSELQDLNSKKpq 84
Cdd:cd17440   159 AYQFHSWRVFVIVCALPCVSSVVALTFMPESPRFLLEVGKHDEAWMILKQIHDTNMRArgQPEKVFTVNRIKKDNTIK-- 236

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1958657956  85 shhiydlvrtrniriitimSIILWLTISVGYFGLSLDTPNL 125
Cdd:cd17440   237 -------------------LTIVWLTLSFGYYGLSVWFPDV 258

Glucose transporter type 8, a Class 3 GLUT, of the Major Facilitator Superfamily of transporters; Glucose transporter type 8 (GLUT8) is also called Solute carrier family 2, facilitated glucose transporter member 8 (SLC2A8) or glucose transporter type X1 (GLUTX1). It is classified as a Class 3 GLUT protein and is an insulin-regulated facilitative glucose transporter predominantly expressed in testis and brain. It can also transport fructose and galactose. SLC2A8 knockout mice were viable, developed normally, and display only a very mild phenotype, including mild alterations in the brain (increased proliferation of hippocampal neurons), heart (impaired transmission of electrical wave through the atrium), and sperm cells (reduced number of motile sperm cells). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1958657956  12 DWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIR 55
Cdd:cd17433   154 DWRWLAVLGSIPPTLMLLLMCFMPETPRFLLTQHRRQEAMAALR 197

Synaptic vesicle glycoprotein 2B of the Major Facilitator Superfamily of transporters; Synaptic vesicle glycoprotein 2 (SV2) is a transporter-like integral membrane glycoprotein, with 12 transmembrane regions, expressed in vertebrates and is localized to synaptic and endocrine secretory vesicles. Three isoforms have been identified, SV2A, SV2B, and SV2C. SV2A and SV2B are widely expressed in the brain, while SV2C is more restricted to evolutionarily older brain. SV2 isoforms have been shown to be critical for the proper function of the central nervous system. SV2 serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. SV2B is a key modulator of amyloid toxicity at the synaptic site and also has an essential role in the formation and maintenance of the glomerular capillary wall. SV2B belongs to the Metazoan Synaptic Vesicle Glycoprotein 2 (SV2) and related small molecule transporter family (SV2-like) of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   8 YFIRDWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAEVIIRKAAKFN--GIVAPSTIFDPSELQDLNSkkpqs 85
Cdd:cd17438   160 YHFHSWRVFVIICALPCIASIIALKFMPESPRFLLEMGKHDEAWMILKNIHDTNmrAKGTPEKVFTVSNIKRKNT----- 234

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1958657956  86 hhiydlvrtrniriitIMSIILWLTISVGYFGLSLDTPNL 125
Cdd:cd17438   235 ----------------LFLAVVWFTMAFSYYGLTVWFPDM 258

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956   1 MVLPLFAYFIRDWRMLLLALTVPGVLCGALWWFIPESPRWLISQGRVKEAE----VIIRKAAKFNGIVAPSTIfdpselq 76
Cdd:cd06174   139 LGGILASSLGFGWRAVFLIAAALALLAAILLLLVVPDPPESARAKNEEASSksvlKLLKRVLKNPGLWLLLLA------- 211

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956  77 dlnskkpqsHHIYDLVrtrniriiTIMSIILWLTISVGYFGLSLDTPNLHGDIYVNCFLLAAVevpayVLAWLLLQHLPR 156
Cdd:cd06174   212 ---------IFLVNLA--------YYSFSTLLPLFLLDLGGLSVAVAGLLLSLFGLAGALGSL-----LLGLLSDRLIGR 269

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958657956 157 RYSISAALFLGGSVLLFIQLVPSelFYLSTALVMVGKFGITSAYSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPY 236
Cdd:cd06174   270 KPLLLIGLLLMALGLALLLLAPS--LLLLLLLLLLLGFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAIGPL 347

                         250       260       270
                  ....*....|....*....|....*....|.
gi 1958657956 237 ---FVYLGAYDRFLPYILMGSLTILTAILTL 264
Cdd:cd06174   348 lagFLLAATFGLTGAFLVLAVLLLLAAILLL 378