crossover junction endonuclease EME1 isoform X1 [Rattus norvegicus]
Protein Classification
List of domain hits
| Name | Accession | Description | Interval | E-value | ||||
| XPF_nuclease-like super family | cl41760 | nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap ... |
1-190 | 3.56e-82 | ||||
nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap endonuclease that act upon 3'-flap structures and involved in DNA repair pathways that are necessary for the removal of UV-light-induced DNA lesions and cross-links between DNA strands. Family members exist either as heterodimers or as homodimers in their functionally competent states which consist of a catalytic and a noncatalytic subunit. The catalytic subunits have a DX(n)RKX(3)D motif. This motif is required for metal-dependent endonuclease activity but not for DNA junction binding. The equivalent regions of the noncatalytic subunits (ERCC1, EME1, and FAAP24) have diverged. The noncatalytic subunits have roles such as binding ssDNA or an ability to target the endonuclease to defined DNA structures or sites of DNA damage. The actual alignment was detected with superfamily member cd20081: Pssm-ID: 425391 Cd Length: 179 Bit Score: 245.82 E-value: 3.56e-82
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| Name | Accession | Description | Interval | E-value | ||||
| XPF_nuclease_EME1 | cd20081 | XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 ... |
1-190 | 3.56e-82 | ||||
XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. The nuclease domain of EME1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA. Pssm-ID: 410857 Cd Length: 179 Bit Score: 245.82 E-value: 3.56e-82
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| Name | Accession | Description | Interval | E-value | ||||
| XPF_nuclease_EME1 | cd20081 | XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 ... |
1-190 | 3.56e-82 | ||||
XPF-like nuclease domain of crossover junction endonuclease EME1; EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. The nuclease domain of EME1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA. Pssm-ID: 410857 Cd Length: 179 Bit Score: 245.82 E-value: 3.56e-82
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| XPF_nuclease_EME-like | cd20080 | XPF-like nuclease domain of the family of Essential Meiotic Endonucleases (EMEs) and similar ... |
1-187 | 4.92e-55 | ||||
XPF-like nuclease domain of the family of Essential Meiotic Endonucleases (EMEs) and similar proteins; The family of EMEs includes EME1 and EME2. EME1, also called MMS4 homolog (hMMS4), interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Its typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks. EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EMEs is a nuclease-like domain which is involved in targeting the MUS81-EME heterodimer complex to DNA. The family also includes budding yeast Mms4 (also known as Eme1 in other organisms), a putative transcriptional (co)activator that protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. It interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The nuclease domain of Mms4 lacks the catalytic motif. Pssm-ID: 410856 Cd Length: 164 Bit Score: 176.42 E-value: 4.92e-55
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| XPF_nuclease_EME | cd20083 | XPF-like nuclease domain of crossover junction endonucleases, EME1, EME2 and similar proteins; ... |
1-187 | 2.04e-37 | ||||
XPF-like nuclease domain of crossover junction endonucleases, EME1, EME2 and similar proteins; The Mus81-EME1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. ERCC4 domain of Eme1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA. Pssm-ID: 410859 Cd Length: 179 Bit Score: 131.25 E-value: 2.04e-37
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| XPF_nuclease_EME2 | cd20082 | XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to ... |
7-187 | 7.61e-31 | ||||
XPF-like nuclease domain of crossover junction endonuclease EME2; EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA. Pssm-ID: 410858 Cd Length: 195 Bit Score: 114.73 E-value: 7.61e-31
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| XPF_nuclease-like | cd19940 | nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap ... |
2-179 | 3.51e-15 | ||||
nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap endonuclease that act upon 3'-flap structures and involved in DNA repair pathways that are necessary for the removal of UV-light-induced DNA lesions and cross-links between DNA strands. Family members exist either as heterodimers or as homodimers in their functionally competent states which consist of a catalytic and a noncatalytic subunit. The catalytic subunits have a DX(n)RKX(3)D motif. This motif is required for metal-dependent endonuclease activity but not for DNA junction binding. The equivalent regions of the noncatalytic subunits (ERCC1, EME1, and FAAP24) have diverged. The noncatalytic subunits have roles such as binding ssDNA or an ability to target the endonuclease to defined DNA structures or sites of DNA damage. Pssm-ID: 410849 [Multi-domain] Cd Length: 126 Bit Score: 70.87 E-value: 3.51e-15
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