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Conserved domains on  [gi|1907170921|ref|XP_036021758|]
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tRNA (guanine(6)-N2)-methyltransferase THUMP3 isoform X1 [Mus musculus]

Protein Classification

RNA methyltransferase( domain architecture ID 10185016)

SAM-dependent RNA methyltransferase containing a THUMP domain, similar to Methanocaldococcus jannaschii Trm14, a tRNA m2G6-methyltransferase and eukaryotic THUMPD3

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
UPF0020 pfam01170
Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated ...
 295-480 3.48e-79

Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated with the THUMP domain that also occurs with RNA modification domains.


:

Pssm-ID: 395932 [Multi-domain]  Cd Length: 184  Bit Score: 245.34  E-value: 3.48e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 295 RNITHF-GPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGATEWSHCYHIAGDNNPLAVNRAANNISSLLTKSQ 373
Cdd:pfam01170   1 RGYRPFnGPAPLKETLAAAMVNLAGWKPGDPLLDPMCGSGTILIEAALMGANIAPGKFDARVRAPLYGSDIDRRMVQGAR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 374 IKDGKTTWGLPIDAVQWDICNLPLRTASVDIIVTDMPFGKRMGSKKRNWNLYPACLREMSRVCRPGtGRAVLLTQDKKCF 453
Cdd:pfam01170  81 LNAENAGVGDLIEFVQADAADLPLLEGSVDVIVTNPPYGIRLGSKGALEALYPEFLREAKRVLRGG-GWLVLLTAENKDF 159

                         170       180
                  ....*....|....*....|....*..
gi 1907170921 454 TKALSGMGhvWRKVHVVWVNIGGLHAA 480
Cdd:pfam01170 160 EKAARERA--WRKKKEFNVHIGGTRVI 184
THUMP_AdoMetMT cd11715
THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of ...
 42-288 9.87e-14

THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of this family contain an N-terminal THUMP domain and a C-terminal S-adenosylmethionine-dependent methyltransferase domain. Members have been implicated in the modification of 23S RNA m2G2445, a highly conserved modification in bacteria and in the m2G6 modification of tRNA. The THUMP domain is named after thiouridine synthases, methylases and PSUSs. The domain consists of about 110 amino acid residues. It is predicted to be an RNA-binding domain and probably functions by delivering a variety of RNA modification enzymes to their targets.


:

Pssm-ID: 212584  Cd Length: 152  Bit Score: 68.76  E-value: 9.87e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921  42 IGATVPTGFEQTAAGEVREKLksACRISKDRGKIYFDIAVESLAQV-HCLRSVDNLFVVVQEFKDYQFKDtkeevlrdFE 120
Cdd:cd11715     1 FFATCPPGLEELLAAELKALG--AEDVEVGPGGVSFEGDLEDAYRAnLWLRTAHRVLLLLAEFEAEDFDD--------LY 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 121 ELAGKLPWSDPLKVWQinttfkkkkakrrkanqsagkekadcgqgdkadekdgkkkhasstsdshildyyenpaikeeis 200
Cdd:cd11715    71 ELAKAIDWEDYLDPDG---------------------------------------------------------------- 86

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 201 tlvgdvlssckdetgqslreetepqvqKFRVTCNRAGEkHCFTSNEAARDFGGAIQEYFK-----WKADMTNFDVEVLLN 275
Cdd:cd11715    87 ---------------------------TFAVRATRVGS-KLFHSQFAALRVKDAIVDRFRekgkrPSVDLDNPDVRIRVH 138

                         250
                  ....*....|...
gi 1907170921 276 IHDNEVIVAIALT 288
Cdd:cd11715   139 LSKDRATLSLDLS 151
 
Name Accession Description Interval E-value
UPF0020 pfam01170
Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated ...
 295-480 3.48e-79

Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated with the THUMP domain that also occurs with RNA modification domains.


Pssm-ID: 395932 [Multi-domain]  Cd Length: 184  Bit Score: 245.34  E-value: 3.48e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 295 RNITHF-GPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGATEWSHCYHIAGDNNPLAVNRAANNISSLLTKSQ 373
Cdd:pfam01170   1 RGYRPFnGPAPLKETLAAAMVNLAGWKPGDPLLDPMCGSGTILIEAALMGANIAPGKFDARVRAPLYGSDIDRRMVQGAR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 374 IKDGKTTWGLPIDAVQWDICNLPLRTASVDIIVTDMPFGKRMGSKKRNWNLYPACLREMSRVCRPGtGRAVLLTQDKKCF 453
Cdd:pfam01170  81 LNAENAGVGDLIEFVQADAADLPLLEGSVDVIVTNPPYGIRLGSKGALEALYPEFLREAKRVLRGG-GWLVLLTAENKDF 159

                         170       180
                  ....*....|....*....|....*..
gi 1907170921 454 TKALSGMGhvWRKVHVVWVNIGGLHAA 480
Cdd:pfam01170 160 EKAARERA--WRKKKEFNVHIGGTRVI 184
Trm11 COG1041
tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 ...
 299-450 5.77e-25

tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 N-methylase Trm11 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440663 [Multi-domain]  Cd Length: 172  Bit Score: 101.18  E-value: 5.77e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 299 HFGPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGAteWSHCYHIAGDNNPLAVNRAANNISSLLTKSqikdgk 378
Cdd:COG1041     4 FFYPGSLDPRLARALVNLAGAKEGDTVLDPFCGTGTILIEAG--LLGRRVIGSDIDPKMVEGARENLEHYGYED------ 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1907170921 379 ttwglpIDAVQWDICNLPLRTASVDIIVTDMPFGKRMGSKKRNW-NLYPACLREMSRVCRPGtGRAVLLTQDK 450
Cdd:COG1041    76 ------ADVIRGDARDLPLADESVDAIVTDPPYGRSSKISGEELlELYEKALEEAARVLKPG-GRVVIVTPRD 141
THUMP_AdoMetMT cd11715
THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of ...
 42-288 9.87e-14

THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of this family contain an N-terminal THUMP domain and a C-terminal S-adenosylmethionine-dependent methyltransferase domain. Members have been implicated in the modification of 23S RNA m2G2445, a highly conserved modification in bacteria and in the m2G6 modification of tRNA. The THUMP domain is named after thiouridine synthases, methylases and PSUSs. The domain consists of about 110 amino acid residues. It is predicted to be an RNA-binding domain and probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 212584  Cd Length: 152  Bit Score: 68.76  E-value: 9.87e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921  42 IGATVPTGFEQTAAGEVREKLksACRISKDRGKIYFDIAVESLAQV-HCLRSVDNLFVVVQEFKDYQFKDtkeevlrdFE 120
Cdd:cd11715     1 FFATCPPGLEELLAAELKALG--AEDVEVGPGGVSFEGDLEDAYRAnLWLRTAHRVLLLLAEFEAEDFDD--------LY 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 121 ELAGKLPWSDPLKVWQinttfkkkkakrrkanqsagkekadcgqgdkadekdgkkkhasstsdshildyyenpaikeeis 200
Cdd:cd11715    71 ELAKAIDWEDYLDPDG---------------------------------------------------------------- 86

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 201 tlvgdvlssckdetgqslreetepqvqKFRVTCNRAGEkHCFTSNEAARDFGGAIQEYFK-----WKADMTNFDVEVLLN 275
Cdd:cd11715    87 ---------------------------TFAVRATRVGS-KLFHSQFAALRVKDAIVDRFRekgkrPSVDLDNPDVRIRVH 138

                         250
                  ....*....|...
gi 1907170921 276 IHDNEVIVAIALT 288
Cdd:cd11715   139 LSKDRATLSLDLS 151
THUMP smart00981
The THUMP domain is named after after thiouridine synthases, methylases and PSUSs; The THUMP ...
 223-285 1.81e-13

The THUMP domain is named after after thiouridine synthases, methylases and PSUSs; The THUMP domain consists of about 110 amino acid residues. The structure of ThiI reveals that the THUMP has a fold unlike that of previously characterised RNA-binding domains. It is predicted that this domain is an RNA-binding domain The THUMP domain probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 214952 [Multi-domain]  Cd Length: 83  Bit Score: 65.76  E-value: 1.81e-13
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1907170921  223 EPQVQKFRVTCNRAGEKHCFTSNEAARDFGGAIQE-YFKWKADMTNFDVEVLLNIHDNEVIVAI 285
Cdd:smart00981  19 FKEGKTFAVRAKRRGKNHEFTSLEVKRAIGDKLLEkTGGRKVDLKNPDVVIRVELRKDKAYLSI 82
THUMP pfam02926
THUMP domain; The THUMP domain is named after after thiouridine synthases, methylases and ...
 203-285 4.24e-11

THUMP domain; The THUMP domain is named after after thiouridine synthases, methylases and PSUSs. The THUMP domain consists of about 110 amino acid residues. The structure of ThiI reveals that the THUMP has a fold unlike that of previously characterized RNA-binding domains. It is predicted that this domain is an RNA-binding domain The THUMP domain probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 460749  Cd Length: 143  Bit Score: 60.91  E-value: 4.24e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 203 VGDVLSSCKDETGQSLREETEPQVQKFRVTCNRAGEKHCFTSNEAARDFGGAIQEYFKWKADMTNFDVEVLLNIHDNEVI 282
Cdd:pfam02926  59 CEADLEDILELAKEIIKDKFKKEGETFAVRVKRRGKNHEFTSLEINREVGKAIVEKTGLKVDLENPDIVVHVEIIKDKAY 138


                  ...
gi 1907170921 283 VAI 285
Cdd:pfam02926 139 ISI 141
TIGR01177 TIGR01177
putative methyltransferase, TIGR01177 family; This family of probable methyltransferases is ...
 244-445 5.01e-11

putative methyltransferase, TIGR01177 family; This family of probable methyltransferases is found exclusively in the Archaea. [Hypothetical proteins, Conserved]


Pssm-ID: 273486 [Multi-domain]  Cd Length: 329  Bit Score: 64.00  E-value: 5.01e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 244 SNEAARDFGGAIQEYfKWKADMTNFDVEVLLNIHDNEVIVAIALT--------EESLHRRniTHFGPTTLRSTLAYGMLR 315
Cdd:TIGR01177 100 KARLERKIGAILKKK-GFKVSLRRPDIVVRVVITEDIFYLGRVLEerdkeqfiERKPDRR--PFFKPGSMDPKLARAMVN 176

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 316 LCEPKPTDVIVDPMCGTGAIPIEGATEWSHCyhIAGDNNPLAVNRAANNisslLTKSQIKDGKTtwglpidaVQWDICNL 395
Cdd:TIGR01177 177 LARVTEGDRVLDPFCGTGGFLIEAGLMGAKV--IGCDIDWKMVAGARIN----LEHYGIEDFFV--------KRGDATKL 242

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1907170921 396 PLRTASVDIIVTDMPFGkRMGSKKRNWN--LYPACLREMSRVCRPGTGRAVL 445
Cdd:TIGR01177 243 PLSSESVDAIATDPPYG-RSTTAAGDGLesLYERSLEEFHEVLKSEGWIVYA 293
rlmL PRK11783
bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2)) ...
 240-340 7.41e-09

bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL;


Pssm-ID: 236981 [Multi-domain]  Cd Length: 702  Bit Score: 58.28  E-value: 7.41e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 240 HCFTSNEAARD--FGG-----AIQEYFKWKA------DMTNFDVEVLLNIHDNEVIVAIALTEESLHRRNI-THFGPTTL 305
Cdd:PRK11783   94 DFSGTNDEIRNtqFGAlkvkdAIVDRFRRKGgprpsvDKEQPDIRINARLNKGEATISLDLSGESLHQRGYrQATGEAPL 173

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1907170921 306 RSTLAYGML-RLCEPKPTDVIVDPMCGTGAIPIEGA 340
Cdd:PRK11783  174 KENLAAAILlRSGWPQEGTPLLDPMCGSGTLLIEAA 209
ThiI COG0301
Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) [Coenzyme ...
 229-283 3.65e-07

Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) [Coenzyme transport and metabolism, Translation, ribosomal structure and biogenesis]; Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) is part of the Pathway/BioSystem: Thiamine biosynthesis


Pssm-ID: 440070 [Multi-domain]  Cd Length: 382  Bit Score: 52.40  E-value: 3.65e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1907170921 229 FRVTCNRAGEKHCFTSNEAARDFGGAIQEYFK-WKADMTNFDVEVLLNIHDNEVIV 283
Cdd:COG0301   103 FKVRAKRAGKHFPFTSPELEREVGGALLENTPgLKVDLKNPDVTIRVEVRDDKAYV 158
 
Name Accession Description Interval E-value
UPF0020 pfam01170
Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated ...
 295-480 3.48e-79

Putative RNA methylase family UPF0020; This domain is probably a methylase. It is associated with the THUMP domain that also occurs with RNA modification domains.


Pssm-ID: 395932 [Multi-domain]  Cd Length: 184  Bit Score: 245.34  E-value: 3.48e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 295 RNITHF-GPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGATEWSHCYHIAGDNNPLAVNRAANNISSLLTKSQ 373
Cdd:pfam01170   1 RGYRPFnGPAPLKETLAAAMVNLAGWKPGDPLLDPMCGSGTILIEAALMGANIAPGKFDARVRAPLYGSDIDRRMVQGAR 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 374 IKDGKTTWGLPIDAVQWDICNLPLRTASVDIIVTDMPFGKRMGSKKRNWNLYPACLREMSRVCRPGtGRAVLLTQDKKCF 453
Cdd:pfam01170  81 LNAENAGVGDLIEFVQADAADLPLLEGSVDVIVTNPPYGIRLGSKGALEALYPEFLREAKRVLRGG-GWLVLLTAENKDF 159

                         170       180
                  ....*....|....*....|....*..
gi 1907170921 454 TKALSGMGhvWRKVHVVWVNIGGLHAA 480
Cdd:pfam01170 160 EKAARERA--WRKKKEFNVHIGGTRVI 184
Trm11 COG1041
tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 ...
 299-450 5.77e-25

tRNA G10 N-methylase Trm11 [Translation, ribosomal structure and biogenesis]; tRNA G10 N-methylase Trm11 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440663 [Multi-domain]  Cd Length: 172  Bit Score: 101.18  E-value: 5.77e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 299 HFGPTTLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGAteWSHCYHIAGDNNPLAVNRAANNISSLLTKSqikdgk 378
Cdd:COG1041     4 FFYPGSLDPRLARALVNLAGAKEGDTVLDPFCGTGTILIEAG--LLGRRVIGSDIDPKMVEGARENLEHYGYED------ 75

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1907170921 379 ttwglpIDAVQWDICNLPLRTASVDIIVTDMPFGKRMGSKKRNW-NLYPACLREMSRVCRPGtGRAVLLTQDK 450
Cdd:COG1041    76 ------ADVIRGDARDLPLADESVDAIVTDPPYGRSSKISGEELlELYEKALEEAARVLKPG-GRVVIVTPRD 141
RlmL COG0116
23S rRNA G2445 N2-methylase RlmL [Translation, ribosomal structure and biogenesis]; 23S rRNA ...
 227-457 1.36e-19

23S rRNA G2445 N2-methylase RlmL [Translation, ribosomal structure and biogenesis]; 23S rRNA G2445 N2-methylase RlmL is part of the Pathway/BioSystem: 23S rRNA modification


Pssm-ID: 439886 [Multi-domain]  Cd Length: 369  Bit Score: 90.16  E-value: 1.36e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 227 QKFRVTCNRAGEKHcFTSNEAARDFGGAIQEYFKWKA------DMTNFDVEVLLNIHDNEVIVAIALTEESLHRRN---I 297
Cdd:COG0116    88 GTFAVDATSVKSKL-FHSQFAALRVKDAIVDRFREKYgarpsvDEDGPDVRIHVHLLKDRATLSLDTSGESLHKRGyreA 166

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 298 THFGPttLRSTLAYGMLRLCEPKPTDVIVDPMCGTGAIPIEGA----------------TEWSHC--------------- 346
Cdd:COG0116   167 QGEAP--LKETLAAALLLLSGWDGDRPLVDPMCGSGTILIEAAliaaniapglnrdfafEKWPDFdaelwqelreeaear 244

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 347 ------YHIAG-DNNPLAVNRAANNISSLltksqikdgkttwGLP--IDAVQWDICNLPlRTASVDIIVTDMPFGKRMGS 417
Cdd:COG0116   245 ikrdppLPIFGsDIDPRAIEAARENAERA-------------GVAdlIEFEQADFRDLE-PPAEPGLIITNPPYGERLGE 310

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|..
gi 1907170921 418 KKRNWNLYpaclREMSRVCRP--GTGRAVLLTQDKKcFTKAL 457
Cdd:COG0116   311 EEELEALY----RELGDVLKQrfKGWSAYILTSDPE-LEKAI 347
THUMP_AdoMetMT cd11715
THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of ...
 42-288 9.87e-14

THUMP domain associated with S-adenosylmethionine-dependent methyltransferases; Proteins of this family contain an N-terminal THUMP domain and a C-terminal S-adenosylmethionine-dependent methyltransferase domain. Members have been implicated in the modification of 23S RNA m2G2445, a highly conserved modification in bacteria and in the m2G6 modification of tRNA. The THUMP domain is named after thiouridine synthases, methylases and PSUSs. The domain consists of about 110 amino acid residues. It is predicted to be an RNA-binding domain and probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 212584  Cd Length: 152  Bit Score: 68.76  E-value: 9.87e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921  42 IGATVPTGFEQTAAGEVREKLksACRISKDRGKIYFDIAVESLAQV-HCLRSVDNLFVVVQEFKDYQFKDtkeevlrdFE 120
Cdd:cd11715     1 FFATCPPGLEELLAAELKALG--AEDVEVGPGGVSFEGDLEDAYRAnLWLRTAHRVLLLLAEFEAEDFDD--------LY 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 121 ELAGKLPWSDPLKVWQinttfkkkkakrrkanqsagkekadcgqgdkadekdgkkkhasstsdshildyyenpaikeeis 200
Cdd:cd11715    71 ELAKAIDWEDYLDPDG---------------------------------------------------------------- 86

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 201 tlvgdvlssckdetgqslreetepqvqKFRVTCNRAGEkHCFTSNEAARDFGGAIQEYFK-----WKADMTNFDVEVLLN 275
Cdd:cd11715    87 ---------------------------TFAVRATRVGS-KLFHSQFAALRVKDAIVDRFRekgkrPSVDLDNPDVRIRVH 138

                         250
                  ....*....|...
gi 1907170921 276 IHDNEVIVAIALT 288
Cdd:cd11715   139 LSKDRATLSLDLS 151
THUMP smart00981
The THUMP domain is named after after thiouridine synthases, methylases and PSUSs; The THUMP ...
 223-285 1.81e-13

The THUMP domain is named after after thiouridine synthases, methylases and PSUSs; The THUMP domain consists of about 110 amino acid residues. The structure of ThiI reveals that the THUMP has a fold unlike that of previously characterised RNA-binding domains. It is predicted that this domain is an RNA-binding domain The THUMP domain probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 214952 [Multi-domain]  Cd Length: 83  Bit Score: 65.76  E-value: 1.81e-13
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1907170921  223 EPQVQKFRVTCNRAGEKHCFTSNEAARDFGGAIQE-YFKWKADMTNFDVEVLLNIHDNEVIVAI 285
Cdd:smart00981  19 FKEGKTFAVRAKRRGKNHEFTSLEVKRAIGDKLLEkTGGRKVDLKNPDVVIRVELRKDKAYLSI 82
THUMP pfam02926
THUMP domain; The THUMP domain is named after after thiouridine synthases, methylases and ...
 203-285 4.24e-11

THUMP domain; The THUMP domain is named after after thiouridine synthases, methylases and PSUSs. The THUMP domain consists of about 110 amino acid residues. The structure of ThiI reveals that the THUMP has a fold unlike that of previously characterized RNA-binding domains. It is predicted that this domain is an RNA-binding domain The THUMP domain probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 460749  Cd Length: 143  Bit Score: 60.91  E-value: 4.24e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 203 VGDVLSSCKDETGQSLREETEPQVQKFRVTCNRAGEKHCFTSNEAARDFGGAIQEYFKWKADMTNFDVEVLLNIHDNEVI 282
Cdd:pfam02926  59 CEADLEDILELAKEIIKDKFKKEGETFAVRVKRRGKNHEFTSLEINREVGKAIVEKTGLKVDLENPDIVVHVEIIKDKAY 138


                  ...
gi 1907170921 283 VAI 285
Cdd:pfam02926 139 ISI 141
TIGR01177 TIGR01177
putative methyltransferase, TIGR01177 family; This family of probable methyltransferases is ...
 244-445 5.01e-11

putative methyltransferase, TIGR01177 family; This family of probable methyltransferases is found exclusively in the Archaea. [Hypothetical proteins, Conserved]


Pssm-ID: 273486 [Multi-domain]  Cd Length: 329  Bit Score: 64.00  E-value: 5.01e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 244 SNEAARDFGGAIQEYfKWKADMTNFDVEVLLNIHDNEVIVAIALT--------EESLHRRniTHFGPTTLRSTLAYGMLR 315
Cdd:TIGR01177 100 KARLERKIGAILKKK-GFKVSLRRPDIVVRVVITEDIFYLGRVLEerdkeqfiERKPDRR--PFFKPGSMDPKLARAMVN 176

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 316 LCEPKPTDVIVDPMCGTGAIPIEGATEWSHCyhIAGDNNPLAVNRAANNisslLTKSQIKDGKTtwglpidaVQWDICNL 395
Cdd:TIGR01177 177 LARVTEGDRVLDPFCGTGGFLIEAGLMGAKV--IGCDIDWKMVAGARIN----LEHYGIEDFFV--------KRGDATKL 242

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1907170921 396 PLRTASVDIIVTDMPFGkRMGSKKRNWN--LYPACLREMSRVCRPGTGRAVL 445
Cdd:TIGR01177 243 PLSSESVDAIATDPPYG-RSTTAAGDGLesLYERSLEEFHEVLKSEGWIVYA 293
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 313-447 1.65e-10

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 59.24  E-value: 1.65e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 313 MLRLCEPKPTDVIVDPMCGTGAIPIEGAtewSHCYHIAG-DNNPLAVNRAANNISSLltksqikdgkttwGLPIDAVQWD 391
Cdd:COG2226    14 LLAALGLRPGARVLDLGCGTGRLALALA---ERGARVTGvDISPEMLELARERAAEA-------------GLNVEFVVGD 77

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1907170921 392 ICNLPLRTASVDIIVTDMPFgkrmgskkRNWNLYPACLREMSRVCRPGtGRAVLLT 447
Cdd:COG2226    78 AEDLPFPDGSFDLVISSFVL--------HHLPDPERALAEIARVLKPG-GRLVVVD 124
THUMP_ThiI cd11716
THUMP domain of thiamine biosynthesis protein ThiI; ThiI is an enzyme responsible for the ...
 194-283 6.28e-09

THUMP domain of thiamine biosynthesis protein ThiI; ThiI is an enzyme responsible for the formation of the modified base S(4)U (4-thiouridine) found at position 8 in some prokaryotic tRNAs. This modification acts as a signal for UV exposure, triggering a response that provides protection against its damaging effects. ThiI consists of an N-terminal THUMP domain, followed by an NFLD domain, and a C-terminal PP-loop pyrophosphatase domain. The N-terminal THUMP domain has been implicated in the recognition of the acceptor-stem region. The THUMP domain is named after thiouridine synthases, methylases and PSUSs. The domain consists of about 110 amino acid residues. It is predicted to be an RNA-binding domain and probably functions by delivering a variety of RNA modification enzymes to their targets.


Pssm-ID: 212585  Cd Length: 166  Bit Score: 55.15  E-value: 6.28e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 194 AIKEEISTLVGDVLSSCKdetgqslreetepqvqKFRVTCNRAGEKHCFTSNEAARDFGGAIQEYFK-WKADMTNFDVEV 272
Cdd:cd11716    83 DIKEAALELLKEELKKGK----------------TFKVRAKRADKSFPFTSMEINREVGAALLENTPdLKVDLKNPDVTI 146

                          90
                  ....*....|.
gi 1907170921 273 LLNIHDNEVIV 283
Cdd:cd11716   147 RVEIREDGAYV 157
rlmL PRK11783
bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2)) ...
 240-340 7.41e-09

bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL;


Pssm-ID: 236981 [Multi-domain]  Cd Length: 702  Bit Score: 58.28  E-value: 7.41e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 240 HCFTSNEAARD--FGG-----AIQEYFKWKA------DMTNFDVEVLLNIHDNEVIVAIALTEESLHRRNI-THFGPTTL 305
Cdd:PRK11783   94 DFSGTNDEIRNtqFGAlkvkdAIVDRFRRKGgprpsvDKEQPDIRINARLNKGEATISLDLSGESLHQRGYrQATGEAPL 173

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1907170921 306 RSTLAYGML-RLCEPKPTDVIVDPMCGTGAIPIEGA 340
Cdd:PRK11783  174 KENLAAAILlRSGWPQEGTPLLDPMCGSGTLLIEAA 209
ThiI COG0301
Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) [Coenzyme ...
 229-283 3.65e-07

Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) [Coenzyme transport and metabolism, Translation, ribosomal structure and biogenesis]; Adenylyl- and sulfurtransferase ThiI (thiamine and tRNA 4-thiouridine biosynthesis) is part of the Pathway/BioSystem: Thiamine biosynthesis


Pssm-ID: 440070 [Multi-domain]  Cd Length: 382  Bit Score: 52.40  E-value: 3.65e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1907170921 229 FRVTCNRAGEKHCFTSNEAARDFGGAIQEYFK-WKADMTNFDVEVLLNIHDNEVIV 283
Cdd:COG0301   103 FKVRAKRAGKHFPFTSPELEREVGGALLENTPgLKVDLKNPDVTIRVEVRDDKAYV 158
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 325-439 4.29e-07

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 47.94  E-value: 4.29e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 325 IVDPMCGTGAIPIEGATEWSHCYhIAGDNNPLAVNRAANNISSLltksqikdgkttwGLPIDAVQWDICNLPLRTASVDI 404
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGARV-TGVDLSPEMLERARERAAEA-------------GLNVEFVQGDAEDLPFPDGSFDL 66

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1907170921 405 IVTDMPFGkrmgskKRNWNLYPACLREMSRVCRPG 439
Cdd:pfam13649  67 VVSSGVLH------HLPDPDLEAALREIARVLKPG 95
ubiE PRK00216
bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol ...
 313-446 3.11e-05

bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE;


Pssm-ID: 234689 [Multi-domain]  Cd Length: 239  Bit Score: 45.53  E-value: 3.11e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 313 MLRLCEPKPTDVIVDPMCGTGAIpiegATEWSHcyhiagdnnplAVNRAANNI-----SSLLTKSQIKDGKTTWGLPIDA 387
Cdd:PRK00216   43 TIKWLGVRPGDKVLDLACGTGDL----AIALAK-----------AVGKTGEVVgldfsEGMLAVGREKLRDLGLSGNVEF 107

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1907170921 388 VQWDICNLPLRTASVDIiVTdMPFGKRmgskkrnwNL--YPACLREMSRVCRPGtGRAVLL 446
Cdd:PRK00216  108 VQGDAEALPFPDNSFDA-VT-IAFGLR--------NVpdIDKALREMYRVLKPG-GRLVIL 157
PRK08317 PRK08317
hypothetical protein; Provisional
 314-449 6.34e-04

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 41.46  E-value: 6.34e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 314 LRLCEPKPTDVIVDPMCGTGAIPIEGATEWSHCYHIAG-DNNP----LAVNRAANNisslltksqikdgkttwGLPIDAV 388
Cdd:PRK08317   12 FELLAVQPGDRVLDVGCGPGNDARELARRVGPEGRVVGiDRSEamlaLAKERAAGL-----------------GPNVEFV 74

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1907170921 389 QWDICNLPLRTASVDIIVTDmpfgkrmgskkrnwNLY------PACLREMSRVCRPGtGRAVLLTQD 449
Cdd:PRK08317   75 RGDADGLPFPDGSFDAVRSD--------------RVLqhledpARALAEIARVLRPG-GRVVVLDTD 126
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 330-445 5.22e-03

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 36.49  E-value: 5.22e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907170921 330 CGTGAIPIEGATEWSHCYHIagDNNPLAVNRAANNISslltksqikdgkttwGLPIDAVQWDICNLPLRTASVDIIVTDM 409
Cdd:pfam08241   5 CGTGLLTELLARLGARVTGV--DISPEMLELAREKAP---------------REGLTFVVGDAEDLPFPDNSFDLVLSSE 67

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1907170921 410 PFGkrmgskkrNWNLYPACLREMSRVCRPGtGRAVL 445
Cdd:pfam08241  68 VLH--------HVEDPERALREIARVLKPG-GILII 94
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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