Colier/Olf/Early B-cell factor (EBF) DNA Binding Domain; COE_DBD is the amino-terminal DNA binding domain of the COE protein family. The COE transcription factor is a regulator of development in several organs and tissues that contain the DBD domain as well as IPT/TIG (immunoglobulin-like, Plexins, transcription factors/transcription factor immunoglobulin) and basic helix-loop-helix (bHLH) domains. COE has four members in mammals (COE1-4) with high sequence similarity at the amino-terminal region. COE_DBD requires a zinc ion to bind DNA and contains a zinc finger motif (H-X(3)-C-X(2)-C-X(5)-C) termed the zinc knuckle. COE is homo- or heterodimerized through the bHLH domain to bind DNA. COE1-4 each has a variant due to alternative splicing. However, this alternative splicing does not occur at the DBD domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034   1 MTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 80
Cdd:cd11606    99 STKQAIPYEGQDKNPEMCRVLLTHEIMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 178

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1622948034  81 VVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 114
Cdd:cd11606   179 VLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 212

Transcription factor COE1 helix-loop-helix domain; This is the helix-loop-helix domain of transcription factor COE1. It is responsible for dimerization.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1622948034 214 LNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALY 257
Cdd:pfam16423   1 LNEPTIDYGFQRLQKLVPRHPGDPERLPKEIILKRAADLAEALY 44

Colier/Olf/Early B-cell factor (EBF) DNA Binding Domain; COE_DBD is the amino-terminal DNA binding domain of the COE protein family. The COE transcription factor is a regulator of development in several organs and tissues that contain the DBD domain as well as IPT/TIG (immunoglobulin-like, Plexins, transcription factors/transcription factor immunoglobulin) and basic helix-loop-helix (bHLH) domains. COE has four members in mammals (COE1-4) with high sequence similarity at the amino-terminal region. COE_DBD requires a zinc ion to bind DNA and contains a zinc finger motif (H-X(3)-C-X(2)-C-X(5)-C) termed the zinc knuckle. COE is homo- or heterodimerized through the bHLH domain to bind DNA. COE1-4 each has a variant due to alternative splicing. However, this alternative splicing does not occur at the DBD domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034   1 MTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 80
Cdd:cd11606    99 STKQAIPYEGQDKNPEMCRVLLTHEIMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 178

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1622948034  81 VVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 114
Cdd:cd11606   179 VLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 212

IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved in the regulation of the early stages of B-cell differentiation, Drosophila collier is a regulator of the head patterning, and a related protein in Xenopus is involved in primary neurogenesis. All COE family members have a well conserved DNA binding domain that contains an atypical Zn finger motif. The function of the IPT domain is unknown.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034 129 PCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGR 208
Cdd:cd01175     1 PCIKAISPSEGWTTGGATVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGR 80

                  ....*
gi 1622948034 209 FIYTA 213
Cdd:cd01175    81 FVYTA 85

IPT/TIG domain; This family consists of a domain that has an immunoglobulin like fold. These domains are found in cell surface receptors such as Met and Ron as well as in intracellular transcription factors where it is involved in DNA binding. CAUTION: This family does not currently recognize a significant number of members.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034 129 PCIKAISPSEGWTTGGATVIIIGDNF---FDGLQVIFGTMLVWSELITPHAIRVQTPPrHIPGVVEVTLSYKSKQFCKGt 205
Cdd:pfam01833   1 PVITSISPSSGPASGGTTITITGSNFgtdSSDLKVTIGGTPCTVISVSSTTIVCTTPP-GTSGLVNVSVTVGGGGISSS- 78

                  ....*.
gi 1622948034 206 PGRFIY 211
Cdd:pfam01833  79 PLTFTY 84

helix loop helix domain;

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|.
gi 1622948034  219 IDYGFQRLQKVIPRHPGDpERLPKEVILKRAADLVEALYGM 259
Cdd:smart00353  11 INEAFDELRSLLPTLPKN-KKLSKAEILRLAIEYIKSLQEE 50

Colier/Olf/Early B-cell factor (EBF) DNA Binding Domain; COE_DBD is the amino-terminal DNA binding domain of the COE protein family. The COE transcription factor is a regulator of development in several organs and tissues that contain the DBD domain as well as IPT/TIG (immunoglobulin-like, Plexins, transcription factors/transcription factor immunoglobulin) and basic helix-loop-helix (bHLH) domains. COE has four members in mammals (COE1-4) with high sequence similarity at the amino-terminal region. COE_DBD requires a zinc ion to bind DNA and contains a zinc finger motif (H-X(3)-C-X(2)-C-X(5)-C) termed the zinc knuckle. COE is homo- or heterodimerized through the bHLH domain to bind DNA. COE1-4 each has a variant due to alternative splicing. However, this alternative splicing does not occur at the DBD domain.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034   1 MTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 80
Cdd:cd11606    99 STKQAIPYEGQDKNPEMCRVLLTHEIMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQV 178

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1622948034  81 VVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 114
Cdd:cd11606   179 VLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRL 212

IPT domain of the COE family (Col/Olf-1/EBF) of non-basic, helix-loop-helix (HLH)-containing transcription factors. COE family proteins are all transcription factors and play an important role in variety of developmental processes. Mouse EBF is involved in the regulation of the early stages of B-cell differentiation, Drosophila collier is a regulator of the head patterning, and a related protein in Xenopus is involved in primary neurogenesis. All COE family members have a well conserved DNA binding domain that contains an atypical Zn finger motif. The function of the IPT domain is unknown.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034 129 PCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGR 208
Cdd:cd01175     1 PCIKAISPSEGWTTGGATVIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGR 80

                  ....*
gi 1622948034 209 FIYTA 213
Cdd:cd01175    81 FVYTA 85

IPT/TIG domain; This family consists of a domain that has an immunoglobulin like fold. These domains are found in cell surface receptors such as Met and Ron as well as in intracellular transcription factors where it is involved in DNA binding. CAUTION: This family does not currently recognize a significant number of members.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034 129 PCIKAISPSEGWTTGGATVIIIGDNF---FDGLQVIFGTMLVWSELITPHAIRVQTPPrHIPGVVEVTLSYKSKQFCKGt 205
Cdd:pfam01833   1 PVITSISPSSGPASGGTTITITGSNFgtdSSDLKVTIGGTPCTVISVSSTTIVCTTPP-GTSGLVNVSVTVGGGGISSS- 78

                  ....*.
gi 1622948034 206 PGRFIY 211
Cdd:pfam01833  79 PLTFTY 84

ig-like, plexins, transcription factors;

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034  129 PCIKAISPSEGWTTGGATVIIIGDNFFDGlQVIFGTMLVWSELIT-----PHAIRVQTPPRH-IPGVVEV-TLSYKSKQF 201
Cdd:smart00429   2 PVITRISPTSGPVSGGTEITLCGKNLKSI-SVVFVEVGVGEAPCTfspssSTAIVCKTPPYHnIPGSVPVrTVGLRNGGV 80

                           90
                   ....*....|.
gi 1622948034  202 cKGTPGRFIYT 212
Cdd:smart00429  81 -PSSPQPFTYV 90

IPT domain of eukaryotic transcription factors NF-kappaB/Rel, nuclear factor of activated Tcells (NFAT), and recombination signal J-kappa binding protein (RBP-Jkappa). The IPT domains in these proteins are involved in DNA binding. Most NF-kappaB/Rel proteins form homo- and heterodimers, while NFAT proteins are largely monomeric (with TonEBP being an exception). While the majority of sequence-specific DNA binding elements are found in the N-terminal domain, several are found in the IPT domain in loops adjacent to, and including, the linker region.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622948034 131 IKAISPSEGWTTGGATVIIIGDNF-FDGLQVIFG----TMLVW-------SELITPHAIRVQTPPRHIPGV-----VEVT 193
Cdd:cd00602     3 ICRVSSLSGSVNGGDEVFLLCDKVnKPDIKVWFGekgpGETVWeaeamfrQEDVRQVAIVFKTPPYHNKWItrpvqVPIQ 82

                          90
                  ....*....|....*....
gi 1622948034 194 LSYKSKQfCKGTPGRFIYT 212
Cdd:cd00602    83 LVRPDDR-KRSEPLTFTYT 100

basic helix-loop-helix (bHLH) domain found in achaete-scute complex-like (ASCL) family; The achaete-scute complex-like (ASCL, also known as achaete-scute complex homolog or ASH) family of bHLH transcription factors, ASCL1-5, have been implicated in cell fate specification and differentiation. They are critical for proper development of the nervous system. The deregulation of ASCL plays a key role in psychiatric and neurological disorders. ASCL-1, also termed Class A basic helix-loop-helix protein 46 (bHLHa46), or achaete-scute homolog 1 (ASH-1), or mammalian achaete-scute homolog 1 (Mash1), is a neural-specific bHLH transcription factor that is expressed in subsets of neural progenitors in both the central and peripheral nervous system. It plays a key role in neuronal differentiation and specification in the nervous system. ASCL-2, also termed achaete-scute homolog 2 (ASH-2), or Class A basic helix-loop-helix protein 45 (bHLHa45), or mammalian achaete-scute homolog 2 (Mash2), is a bHLH transcription factor that is involved in Schwann cell differentiation and control of proliferation in adult peripheral nerves. ASCL-3, also termed Class A basic helix-loop-helix protein 42 (bHLHa42), or bHLH transcriptional regulator Sgn-1, or achaete-scute homolog 3 (ASH-3), is a bHLH transcription factor specifically localized in the duct cells of the salivary glands. It may act as transcriptional repressor that inhibits myogenesis. The family also includes Drosophila melanogaster achaete-scute complex (AS-C) proteins, which consists of lethal of scute (also known as achaete-scute complex protein T3 or AST3), scute (also known as achaete-scute complex protein T4 or AST4), achaete (also known as achaete-scute complex protein T5 or AST5), and asense (also known as achaete-scute complex protein T8 or AST8). They are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system, as well as in sex determination and dosage compensation.

                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 1622948034 222 GFQRLQKVIPrHPGDPERLPKEVILKRAADLVEALYGM 259
Cdd:cd11418    19 AFDRLRQHVP-YLKRRKKLSKVKTLRRAIEYIRHLQKL 55