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Conserved domains on  [gi|1622910833|ref|XP_028696353|]
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protein VAC14 homolog isoform X1 [Macaca mulatta]

Protein Classification

VAC14 family protein( domain architecture ID 10579327)

VAC14 family protein similar to Saccharomyces cerevisiae vacuole morphology and inheritance protein 14 (VAC14) that is a component of the PI(3,5)P2 regulatory complex that regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate

Gene Ontology:  GO:0070772|GO:0006661|GO:0005515
PubMed:  23389034|19037259|35552740|10378263
SCOP:  4001283

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Vac14_Fig4_bd pfam11916
Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, ...
 546-682 1.27e-79

Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. The C-terminal region of Vac14 binds to Fig4p. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


:

Pssm-ID: 463395  Cd Length: 179  Bit Score: 253.56  E-value: 1.27e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 546 RKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCL 625
Cdd:pfam11916   1 RRLLERRGSLIIRQLCLLLNAERIYRTLAEILEKEEDLEFASTMVQTLNTILLTSPELAELRNKLRNLDSEEDRELFSTL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1622910833 626 YRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFT 682
Cdd:pfam11916  81 YKSWCHNPVATLSLCLLAQAYEHAYNLLQSFGELEITVEFLVQIDKLVQLLESPVFT 137
Vac14_Fab1_bd pfam12755
Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex ...
 67-163 8.94e-56

Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. This domain appears to be the one responsible for binding to Fab1. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


:

Pssm-ID: 403838  Cd Length: 97  Bit Score: 186.26  E-value: 8.94e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833  67 HSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADP 146
Cdd:pfam12755   1 NARKGGLIGLAATAIALGKDIAPYLDDIIPPVLACFSDQDSRVRYYACESLYNIAKVARGEVLPYFNDIFDGLCKLFADS 80

                          90
                  ....*....|....*..
gi 1622910833 147 DPNVKSGSELLDRLLKD 163
Cdd:pfam12755  81 DPSVKNGAELLDRLLKD 97
PRK07764 super family cl35613
DNA polymerase III subunits gamma and tau; Validated
 693-801 2.31e-04

DNA polymerase III subunits gamma and tau; Validated


The actual alignment was detected with superfamily member PRK07764:

Pssm-ID: 236090 [Multi-domain]  Cd Length: 824  Bit Score: 44.98  E-value: 2.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 693 ARAEPTGESVTLRAPSPHITREAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQGPLRPAHAPAAEQRLPA--A 770
Cdd:PRK07764  635 APAEASAAPAPGVAAPEHHPKHVAVPDASDGGDGWPAKAGGAAPAAPPPAPAPAAPAAPAGAAPAQPAPAPAATPPAgqA 714

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1622910833 771 LTPAPVRAQPRAAADRRQSKGSPQVPESRLP 801
Cdd:PRK07764  715 DDPAAQPPQAAQGASAPSPAADDPVPLPPEP 745
 
Name Accession Description Interval E-value
Vac14_Fig4_bd pfam11916
Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, ...
 546-682 1.27e-79

Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. The C-terminal region of Vac14 binds to Fig4p. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


Pssm-ID: 463395  Cd Length: 179  Bit Score: 253.56  E-value: 1.27e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 546 RKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCL 625
Cdd:pfam11916   1 RRLLERRGSLIIRQLCLLLNAERIYRTLAEILEKEEDLEFASTMVQTLNTILLTSPELAELRNKLRNLDSEEDRELFSTL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1622910833 626 YRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFT 682
Cdd:pfam11916  81 YKSWCHNPVATLSLCLLAQAYEHAYNLLQSFGELEITVEFLVQIDKLVQLLESPVFT 137
Vac14_Fab1_bd pfam12755
Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex ...
 67-163 8.94e-56

Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. This domain appears to be the one responsible for binding to Fab1. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


Pssm-ID: 403838  Cd Length: 97  Bit Score: 186.26  E-value: 8.94e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833  67 HSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADP 146
Cdd:pfam12755   1 NARKGGLIGLAATAIALGKDIAPYLDDIIPPVLACFSDQDSRVRYYACESLYNIAKVARGEVLPYFNDIFDGLCKLFADS 80

                          90
                  ....*....|....*..
gi 1622910833 147 DPNVKSGSELLDRLLKD 163
Cdd:pfam12755  81 DPSVKNGAELLDRLLKD 97
PRK07764 PRK07764
DNA polymerase III subunits gamma and tau; Validated
 693-801 2.31e-04

DNA polymerase III subunits gamma and tau; Validated


Pssm-ID: 236090 [Multi-domain]  Cd Length: 824  Bit Score: 44.98  E-value: 2.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 693 ARAEPTGESVTLRAPSPHITREAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQGPLRPAHAPAAEQRLPA--A 770
Cdd:PRK07764  635 APAEASAAPAPGVAAPEHHPKHVAVPDASDGGDGWPAKAGGAAPAAPPPAPAPAAPAAPAGAAPAQPAPAPAATPPAgqA 714

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1622910833 771 LTPAPVRAQPRAAADRRQSKGSPQVPESRLP 801
Cdd:PRK07764  715 DDPAAQPPQAAQGASAPSPAADDPVPLPPEP 745
 
Name Accession Description Interval E-value
Vac14_Fig4_bd pfam11916
Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, ...
 546-682 1.27e-79

Vacuolar protein 14 C-terminal Fig4p binding; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. The C-terminal region of Vac14 binds to Fig4p. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


Pssm-ID: 463395  Cd Length: 179  Bit Score: 253.56  E-value: 1.27e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 546 RKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCL 625
Cdd:pfam11916   1 RRLLERRGSLIIRQLCLLLNAERIYRTLAEILEKEEDLEFASTMVQTLNTILLTSPELAELRNKLRNLDSEEDRELFSTL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1622910833 626 YRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFT 682
Cdd:pfam11916  81 YKSWCHNPVATLSLCLLAQAYEHAYNLLQSFGELEITVEFLVQIDKLVQLLESPVFT 137
Vac14_Fab1_bd pfam12755
Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex ...
 67-163 8.94e-56

Vacuolar 14 Fab1-binding region; Vac14 is a scaffold for the Fab1 kinase complex, a complex that allows for the dynamic interconversion of PI3P and PI(3,5)P2p (phosphoinositide phosphate (PIP) lipids, that are generated transiently on the cytoplasmic face of selected intracellular membranes). This interconversion is regulated by at least five proteins in yeast: the lipid kinase Fab1p, lipid phosphatase Fig4p, the Fab1p activator Vac7p, the Fab1p inhibitor Atg18p, and Vac14p, a protein required for the activity of both Fab1p and Fig4p. This domain appears to be the one responsible for binding to Fab1. The full length Vac14 in yeasts is likely to be a protein carrying a succession of HEAT repeats, most of which have now degenerated. This regulatory system is crucial for the proper functioning of the mammalian nervous system.


Pssm-ID: 403838  Cd Length: 97  Bit Score: 186.26  E-value: 8.94e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833  67 HSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADP 146
Cdd:pfam12755   1 NARKGGLIGLAATAIALGKDIAPYLDDIIPPVLACFSDQDSRVRYYACESLYNIAKVARGEVLPYFNDIFDGLCKLFADS 80

                          90
                  ....*....|....*..
gi 1622910833 147 DPNVKSGSELLDRLLKD 163
Cdd:pfam12755  81 DPSVKNGAELLDRLLKD 97
PRK07764 PRK07764
DNA polymerase III subunits gamma and tau; Validated
 693-801 2.31e-04

DNA polymerase III subunits gamma and tau; Validated


Pssm-ID: 236090 [Multi-domain]  Cd Length: 824  Bit Score: 44.98  E-value: 2.31e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 693 ARAEPTGESVTLRAPSPHITREAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQGPLRPAHAPAAEQRLPA--A 770
Cdd:PRK07764  635 APAEASAAPAPGVAAPEHHPKHVAVPDASDGGDGWPAKAGGAAPAAPPPAPAPAAPAAPAGAAPAQPAPAPAATPPAgqA 714

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1622910833 771 LTPAPVRAQPRAAADRRQSKGSPQVPESRLP 801
Cdd:PRK07764  715 DDPAAQPPQAAQGASAPSPAADDPVPLPPEP 745
PHA03247 PHA03247
large tegument protein UL36; Provisional
 685-796 2.35e-03

large tegument protein UL36; Provisional


Pssm-ID: 223021 [Multi-domain]  Cd Length: 3151  Bit Score: 41.85  E-value: 2.35e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833  685 PIYSSPVLARAEPTGESVtlrAPSPHITREAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQGPLRPAHAPAAE 764
Cdd:PHA03247  2839 PPPPPGPPPPSLPLGGSV---APGGDVRRRPPSRSPAAKPAAPARPPVRRLARPAVSRSTESFALPPDQPERPPQPQAPP 2915

                           90       100       110
                   ....*....|....*....|....*....|..
gi 1622910833  765 QRLPAALTPAPVRAQPRAaadrrQSKGSPQVP 796
Cdd:PHA03247  2916 PPQPQPQPPPPPQPQPPP-----PPPPRPQPP 2942
PRK07003 PRK07003
DNA polymerase III subunit gamma/tau;
693-801 2.41e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 235906 [Multi-domain]  Cd Length: 830  Bit Score: 41.37  E-value: 2.41e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 693 ARAEPTGESVTLRAPSPHITREAAEQGAgnrPGPTAhlgVVGLRSAPAAAGREEQPLLDQGPLRPAHAPAaeqrlPAALT 772
Cdd:PRK07003  416 AAAAATRAEAPPAAPAPPATADRGDDAA---DGDAP---VPAKANARASADSRCDERDAQPPADSGSASA-----PASDA 484

                          90       100
                  ....*....|....*....|....*....
gi 1622910833 773 PAPVRAQPRAAADRRQSKGSPQVPESRLP 801
Cdd:PRK07003  485 PPDAAFEPAPRAAAPSAATPAAVPDARAP 513
PRK07003 PRK07003
DNA polymerase III subunit gamma/tau;
677-791 2.95e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 235906 [Multi-domain]  Cd Length: 830  Bit Score: 41.37  E-value: 2.95e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 677 ECPIFTSHPIYSSPVLARAEPTGESVTLRAPSPHITrEAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQG--- 753
Cdd:PRK07003  424 EAPPAAPAPPATADRGDDAADGDAPVPAKANARASA-DSRCDERDAQPPADSGSASAPASDAPPDAAFEPAPRAAAPsaa 502

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1622910833 754 ---PLRPAHAPAAEQR---LPAALTPAPVRAQPRAAADRRQSKG 791
Cdd:PRK07003  503 tpaAVPDARAPAAASRedaPAAAAPPAPEARPPTPAAAAPAARA 546
PRK12323 PRK12323
DNA polymerase III subunit gamma/tau;
688-801 5.18e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 237057 [Multi-domain]  Cd Length: 700  Bit Score: 40.24  E-value: 5.18e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622910833 688 SSPVLARAEPtgesVTLRAPSPHITREAAEQGAGNRPGPTAHLGVVGLRSAPAAAGREEQPLLDQGPL---RPAHAPAAE 764
Cdd:PRK12323  372 AGPATAAAAP----VAQPAPAAAAPAAAAPAPAAPPAAPAAAPAAAAAARAVAAAPARRSPAPEALAAarqASARGPGGA 447

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1622910833 765 QRLPAALTPAPVRAQPRAAAD-RRQSKGSPQVPESRLP 801
Cdd:PRK12323  448 PAPAPAPAAAPAAAARPAAAGpRPVAAAAAAAPARAAP 485
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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