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Conserved domains on  [gi|1622876673|ref|XP_028691723|]
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cystinosin isoform X2 [Macaca mulatta]

Protein Classification

cystinosin/MPDU1 family protein( domain architecture ID 11490013)

cystinosin/MPDU1 family protein similar to homo sapiens mannose-P-dolichol utilization defect 1, a cystinosin homolog that required for normal utilization of mannose-dolichol phosphate (Dol-P-Man) in the synthesis of N-linked and O-linked oligosaccharides and GPI anchors

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 125-285 4.08e-73

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


:

Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 227.05  E-value: 4.08e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 125 IVNQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNgvnpVNSN 204
Cdd:TIGR00951   2 LLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFNFLQLYCWSITNEFPLSSPG----VTQN 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 205 DVFFSLHAVVLTLIIIVQCCLYERGGQRVSWPAVGFLVLAWLFALVTMIVAAVGVTTWLQFLFCFSYIKLAVTLVKYFPQ 284
Cdd:TIGR00951  78 DVFFTLHAILICFIVLHQCGDYERGWQRVSNPWILRILVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVKYFPQ 157


                  .
gi 1622876673 285 V 285
Cdd:TIGR00951 158 A 158
PHA03378 super family cl33729
EBNA-3B; Provisional
 283-376 8.82e-05

EBNA-3B; Provisional


The actual alignment was detected with superfamily member PHA03378:

Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 44.67  E-value: 8.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 283 PQVLPGPcshgrwqERREVQGPGPAPVGQLRPGRGNRTESHREP-----GSPGGAARWGGQPTALPPGWNHTRSLAEAPG 357
Cdd:PHA03378  707 PAAPPGR-------AQRPAAATGRARPPAAAPGRARPPAAAPGRarppaAAPGRARPPAAAPGRARPPAAAPGAPTPQPP 779

                          90
                  ....*....|....*....
gi 1622876673 358 PHAPLSIRRCAGGSPSPHP 376
Cdd:PHA03378  780 PQAPPAPQQRPRGAPTPQP 798
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 125-285 4.08e-73

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 227.05  E-value: 4.08e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 125 IVNQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNgvnpVNSN 204
Cdd:TIGR00951   2 LLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFNFLQLYCWSITNEFPLSSPG----VTQN 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 205 DVFFSLHAVVLTLIIIVQCCLYERGGQRVSWPAVGFLVLAWLFALVTMIVAAVGVTTWLQFLFCFSYIKLAVTLVKYFPQ 284
Cdd:TIGR00951  78 DVFFTLHAILICFIVLHQCGDYERGWQRVSNPWILRILVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVKYFPQ 157


                  .
gi 1622876673 285 V 285
Cdd:TIGR00951 158 A 158
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 126-186 2.05e-18

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 78.31  E-value: 2.05e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1622876673 126 VNQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYI 186
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPSL 61
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 140-171 1.75e-08

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 49.76  E-value: 1.75e-08
                           10        20        30
                   ....*....|....*....|....*....|..
gi 1622876673  140 ISFYPQVIMNWRRKSVIGLSFDFVALNLTGFV 171
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
PHA03378 PHA03378
EBNA-3B; Provisional
 283-376 8.82e-05

EBNA-3B; Provisional


Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 44.67  E-value: 8.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 283 PQVLPGPcshgrwqERREVQGPGPAPVGQLRPGRGNRTESHREP-----GSPGGAARWGGQPTALPPGWNHTRSLAEAPG 357
Cdd:PHA03378  707 PAAPPGR-------AQRPAAATGRARPPAAAPGRARPPAAAPGRarppaAAPGRARPPAAAPGRARPPAAAPGAPTPQPP 779

                          90
                  ....*....|....*....
gi 1622876673 358 PHAPLSIRRCAGGSPSPHP 376
Cdd:PHA03378  780 PQAPPAPQQRPRGAPTPQP 798
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 125-285 4.08e-73

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 227.05  E-value: 4.08e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 125 IVNQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYIKEQFLLKYPNgvnpVNSN 204
Cdd:TIGR00951   2 LLSQILGWGYVAAWSISFYPQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFNFLQLYCWSITNEFPLSSPG----VTQN 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 205 DVFFSLHAVVLTLIIIVQCCLYERGGQRVSWPAVGFLVLAWLFALVTMIVAAVGVTTWLQFLFCFSYIKLAVTLVKYFPQ 284
Cdd:TIGR00951  78 DVFFTLHAILICFIVLHQCGDYERGWQRVSNPWILRILVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVKYFPQ 157


                  .
gi 1622876673 285 V 285
Cdd:TIGR00951 158 A 158
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 126-186 2.05e-18

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 78.31  E-value: 2.05e-18
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1622876673 126 VNQVIGWIYFVAWSISFYPQVIMNWRRKSVIGLSFDFVALNLTGFVAYSVFNIGLLWVPYI 186
Cdd:pfam04193   1 LSNVLGYISLVLWLVSYLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPSL 61
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 140-171 1.75e-08

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 49.76  E-value: 1.75e-08
                           10        20        30
                   ....*....|....*....|....*....|..
gi 1622876673  140 ISFYPQVIMNWRRKSVIGLSFDFVALNLTGFV 171
Cdd:smart00679   1 VSLLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
PHA03378 PHA03378
EBNA-3B; Provisional
 283-376 8.82e-05

EBNA-3B; Provisional


Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 44.67  E-value: 8.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622876673 283 PQVLPGPcshgrwqERREVQGPGPAPVGQLRPGRGNRTESHREP-----GSPGGAARWGGQPTALPPGWNHTRSLAEAPG 357
Cdd:PHA03378  707 PAAPPGR-------AQRPAAATGRARPPAAAPGRARPPAAAPGRarppaAAPGRARPPAAAPGRARPPAAAPGAPTPQPP 779

                          90
                  ....*....|....*....
gi 1622876673 358 PHAPLSIRRCAGGSPSPHP 376
Cdd:PHA03378  780 PQAPPAPQQRPRGAPTPQP 798
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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