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Conserved domains on  [gi|1622874167|ref|XP_028691267|]
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astrotactin-2 isoform X6 [Macaca mulatta]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ASTN1_2_EGF_Fn pfam19743
ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane ...
 16-231 3.24e-160

ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane proteins with a large C-terminal domain, extracellular for ASTN1 and endosome luminal for ASTN-2. They play critical roles in neurodevelopment, and ASTN-2 is also involved in the planar cell polarity pathway in hair cells. This is a domain found in the middle of the C-terminal of ASTN-1 and 2, which comprises a EGF- like domain (EGF4) and a fibronectin type III (Fn(III)) domain. These subdomains are located between the MACPF and annexin- like domains. The structure of Fn(III) from ASTN2 revealed an unexpected feature which has two additional beta strands folded across the core. The junction between EGF-4 and Fn(III) domains in ASTN2 (but not in ASTN1) is thought to be an inositol triphosphate binding site.


:

Pssm-ID: 437575  Cd Length: 216  Bit Score: 448.60  E-value: 3.24e-160
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  16 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKSALMSSYWCSGKGDVI 95
Cdd:pfam19743   1 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNVTKEAFKSALMSSYWCSGKGDVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  96 DDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 175
Cdd:pfam19743  81 DDWCRCDLSAFDKDGLPNCSPLPQPVLRLSPYVEPSSTVVSLEWMDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1622874167 176 FADDLLSGLGTSCVAAGRSHGEIPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 231
Cdd:pfam19743 161 FADDLLSGLGTSCVAAGRSHGDVPESSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 216
Annexin_like pfam18411
Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an ...
 242-334 8.95e-64

Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. The annexin-like domain is closest in fold to repeat three of human annexin V and similarly binds calcium, yet shares no sequence homology with it. Notably, this ASTN-2 annexin-like domain is closer in structure to human annexin repeat 3 than human annexin repeat 3 is to repeat 1. Annexin-like domains are known for their capacity to remodel membranes, triggered by calcium binding, and have also been suggested to be involved in the formation of pores in membranes both are possible biological roles of the ASTN-2 annexin-like domain.


:

Pssm-ID: 465756  Cd Length: 93  Bit Score: 198.93  E-value: 8.95e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167 242 CPLVDDNKAEEIADKIYNLYNGYTSGKEQQMAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRR 321
Cdd:pfam18411   1 CPLVDDSKAEEIADKIYNLYNGYTSGKEQQTAYNTLMELGAPGLNRVQHHYNQHYEKFGEFVWRSEDELGPRKAHLILRR 80

                          90
                  ....*....|...
gi 1622874167 322 LERVSSHCSSLLR 334
Cdd:pfam18411  81 LEKVSSHCSSLLR 93
ASTN_2_hairpin pfam18577
Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the ...
 338-384 2.09e-26

Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the C-terminal region of astrotactin 2 proteins (ASTN-2). ASTN-2 is an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. it consists of multiple polypeptide folds: a perforin-like domain, a minimal epidermal growth factor-like module, a fibronectin type III domain Fn (III) and an annexin-like domain as well as the beta hairpin domain which packs across the fibronectin domain.


:

Pssm-ID: 436591  Cd Length: 47  Bit Score: 99.91  E-value: 2.09e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 1622874167 338 IQSRVETVPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNT 384
Cdd:pfam18577   1 IQSRTDTVPYLFCRSEEVRPPGMVWHSSLKDTKVTCEEKLMSMPRNT 47
 
Name Accession Description Interval E-value
ASTN1_2_EGF_Fn pfam19743
ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane ...
 16-231 3.24e-160

ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane proteins with a large C-terminal domain, extracellular for ASTN1 and endosome luminal for ASTN-2. They play critical roles in neurodevelopment, and ASTN-2 is also involved in the planar cell polarity pathway in hair cells. This is a domain found in the middle of the C-terminal of ASTN-1 and 2, which comprises a EGF- like domain (EGF4) and a fibronectin type III (Fn(III)) domain. These subdomains are located between the MACPF and annexin- like domains. The structure of Fn(III) from ASTN2 revealed an unexpected feature which has two additional beta strands folded across the core. The junction between EGF-4 and Fn(III) domains in ASTN2 (but not in ASTN1) is thought to be an inositol triphosphate binding site.


Pssm-ID: 437575  Cd Length: 216  Bit Score: 448.60  E-value: 3.24e-160
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  16 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKSALMSSYWCSGKGDVI 95
Cdd:pfam19743   1 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNVTKEAFKSALMSSYWCSGKGDVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  96 DDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 175
Cdd:pfam19743  81 DDWCRCDLSAFDKDGLPNCSPLPQPVLRLSPYVEPSSTVVSLEWMDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1622874167 176 FADDLLSGLGTSCVAAGRSHGEIPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 231
Cdd:pfam19743 161 FADDLLSGLGTSCVAAGRSHGDVPESSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 216
Annexin_like pfam18411
Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an ...
 242-334 8.95e-64

Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. The annexin-like domain is closest in fold to repeat three of human annexin V and similarly binds calcium, yet shares no sequence homology with it. Notably, this ASTN-2 annexin-like domain is closer in structure to human annexin repeat 3 than human annexin repeat 3 is to repeat 1. Annexin-like domains are known for their capacity to remodel membranes, triggered by calcium binding, and have also been suggested to be involved in the formation of pores in membranes both are possible biological roles of the ASTN-2 annexin-like domain.


Pssm-ID: 465756  Cd Length: 93  Bit Score: 198.93  E-value: 8.95e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167 242 CPLVDDNKAEEIADKIYNLYNGYTSGKEQQMAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRR 321
Cdd:pfam18411   1 CPLVDDSKAEEIADKIYNLYNGYTSGKEQQTAYNTLMELGAPGLNRVQHHYNQHYEKFGEFVWRSEDELGPRKAHLILRR 80

                          90
                  ....*....|...
gi 1622874167 322 LERVSSHCSSLLR 334
Cdd:pfam18411  81 LEKVSSHCSSLLR 93
ASTN_2_hairpin pfam18577
Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the ...
 338-384 2.09e-26

Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the C-terminal region of astrotactin 2 proteins (ASTN-2). ASTN-2 is an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. it consists of multiple polypeptide folds: a perforin-like domain, a minimal epidermal growth factor-like module, a fibronectin type III domain Fn (III) and an annexin-like domain as well as the beta hairpin domain which packs across the fibronectin domain.


Pssm-ID: 436591  Cd Length: 47  Bit Score: 99.91  E-value: 2.09e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 1622874167 338 IQSRVETVPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNT 384
Cdd:pfam18577   1 IQSRTDTVPYLFCRSEEVRPPGMVWHSSLKDTKVTCEEKLMSMPRNT 47
MACPF smart00457
membrane-attack complex / perforin;
1-87 7.07e-11

membrane-attack complex / perforin;


Pssm-ID: 214671  Cd Length: 195  Bit Score: 60.91  E-value: 7.07e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167    1 MPFITYLSGLLTAQ-MLSDDQLISG-VEIRCEEKgRCPSTCHLCRRPGKEQLSPTPVLLEInRVVPLYTLIQDN----GT 74
Cdd:smart00457 105 LQSSSYIKYLSTSLrRESHTQVLGGhVTVLCDLL-RGPSSNSLDFSDWAESVPNEPVLIDV-SLAPIYELLPPNpelsQK 182

                           90
                   ....*....|...
gi 1622874167   75 KEAFKSALMSSYW 87
Cdd:smart00457 183 REALRQALRSYLK 195
FN3 cd00063
Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein ...
 118-240 5.96e-03

Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.


Pssm-ID: 238020 [Multi-domain]  Cd Length: 93  Bit Score: 35.94  E-value: 5.96e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167 118 PQPVLRLSPTVEPSSTVVsLEWvDVQPAIGTKVSDYILQHKKVDEYTDTDLytgeflsfaddllsglgtscvaagrshgE 197
Cdd:cd00063     1 PSPPTNLRVTDVTSTSVT-LSW-TPPEDDGGPITGYVVEYREKGSGDWKEV----------------------------E 50

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1622874167 198 IPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRT 240
Cdd:cd00063    51 VTPGSETSYTLTGLKPGTEYEFRVRAVNGGGESPPSESVTVTT 93
 
Name Accession Description Interval E-value
ASTN1_2_EGF_Fn pfam19743
ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane ...
 16-231 3.24e-160

ASTN1/2 EGF-like and Fn(III) domains; Astrotactin-1 and 2 (ASTN1/2) are integral membrane proteins with a large C-terminal domain, extracellular for ASTN1 and endosome luminal for ASTN-2. They play critical roles in neurodevelopment, and ASTN-2 is also involved in the planar cell polarity pathway in hair cells. This is a domain found in the middle of the C-terminal of ASTN-1 and 2, which comprises a EGF- like domain (EGF4) and a fibronectin type III (Fn(III)) domain. These subdomains are located between the MACPF and annexin- like domains. The structure of Fn(III) from ASTN2 revealed an unexpected feature which has two additional beta strands folded across the core. The junction between EGF-4 and Fn(III) domains in ASTN2 (but not in ASTN1) is thought to be an inositol triphosphate binding site.


Pssm-ID: 437575  Cd Length: 216  Bit Score: 448.60  E-value: 3.24e-160
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  16 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKSALMSSYWCSGKGDVI 95
Cdd:pfam19743   1 LSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNVTKEAFKSALMSSYWCSGKGDVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167  96 DDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 175
Cdd:pfam19743  81 DDWCRCDLSAFDKDGLPNCSPLPQPVLRLSPYVEPSSTVVSLEWMDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLS 160

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1622874167 176 FADDLLSGLGTSCVAAGRSHGEIPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 231
Cdd:pfam19743 161 FADDLLSGLGTSCVAAGRSHGDVPESSIYSVIFKCLEPDGLYKFTLYAVDTRGRHS 216
Annexin_like pfam18411
Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an ...
 242-334 8.95e-64

Annexin-like domain; This annexin-like domain can be found in astrotactin 2 (Astn-2), an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. The annexin-like domain is closest in fold to repeat three of human annexin V and similarly binds calcium, yet shares no sequence homology with it. Notably, this ASTN-2 annexin-like domain is closer in structure to human annexin repeat 3 than human annexin repeat 3 is to repeat 1. Annexin-like domains are known for their capacity to remodel membranes, triggered by calcium binding, and have also been suggested to be involved in the formation of pores in membranes both are possible biological roles of the ASTN-2 annexin-like domain.


Pssm-ID: 465756  Cd Length: 93  Bit Score: 198.93  E-value: 8.95e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167 242 CPLVDDNKAEEIADKIYNLYNGYTSGKEQQMAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRR 321
Cdd:pfam18411   1 CPLVDDSKAEEIADKIYNLYNGYTSGKEQQTAYNTLMELGAPGLNRVQHHYNQHYEKFGEFVWRSEDELGPRKAHLILRR 80

                          90
                  ....*....|...
gi 1622874167 322 LERVSSHCSSLLR 334
Cdd:pfam18411  81 LEKVSSHCSSLLR 93
ASTN_2_hairpin pfam18577
Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the ...
 338-384 2.09e-26

Astrotactin-2 C-terminal beta-hairpin domain; This is a beta-hairpin domain found at the C-terminal region of astrotactin 2 proteins (ASTN-2). ASTN-2 is an integral membrane perforin-like protein linked to the planar cell polarity pathway in hair cells. it consists of multiple polypeptide folds: a perforin-like domain, a minimal epidermal growth factor-like module, a fibronectin type III domain Fn (III) and an annexin-like domain as well as the beta hairpin domain which packs across the fibronectin domain.


Pssm-ID: 436591  Cd Length: 47  Bit Score: 99.91  E-value: 2.09e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 1622874167 338 IQSRVETVPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNT 384
Cdd:pfam18577   1 IQSRTDTVPYLFCRSEEVRPPGMVWHSSLKDTKVTCEEKLMSMPRNT 47
MACPF smart00457
membrane-attack complex / perforin;
1-87 7.07e-11

membrane-attack complex / perforin;


Pssm-ID: 214671  Cd Length: 195  Bit Score: 60.91  E-value: 7.07e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167    1 MPFITYLSGLLTAQ-MLSDDQLISG-VEIRCEEKgRCPSTCHLCRRPGKEQLSPTPVLLEInRVVPLYTLIQDN----GT 74
Cdd:smart00457 105 LQSSSYIKYLSTSLrRESHTQVLGGhVTVLCDLL-RGPSSNSLDFSDWAESVPNEPVLIDV-SLAPIYELLPPNpelsQK 182

                           90
                   ....*....|...
gi 1622874167   75 KEAFKSALMSSYW 87
Cdd:smart00457 183 REALRQALRSYLK 195
FN3 cd00063
Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein ...
 118-240 5.96e-03

Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all animal proteins contain the FN3 repeat; including extracellular and intracellular proteins, membrane spanning cytokine receptors, growth hormone receptors, tyrosine phosphatase receptors, and adhesion molecules. FN3-like domains are also found in bacterial glycosyl hydrolases.


Pssm-ID: 238020 [Multi-domain]  Cd Length: 93  Bit Score: 35.94  E-value: 5.96e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622874167 118 PQPVLRLSPTVEPSSTVVsLEWvDVQPAIGTKVSDYILQHKKVDEYTDTDLytgeflsfaddllsglgtscvaagrshgE 197
Cdd:cd00063     1 PSPPTNLRVTDVTSTSVT-LSW-TPPEDDGGPITGYVVEYREKGSGDWKEV----------------------------E 50

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1622874167 198 IPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRT 240
Cdd:cd00063    51 VTPGSETSYTLTGLKPGTEYEFRVRAVNGGGESPPSESVTVTT 93
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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