calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVAD 93
Cdd:cd21207     1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                          90       100
                  ....*....|....*....|....*..
gi 1339089646  94 VDVFQTVDLWEKKDIAQVTTTLFALGR 120
Cdd:cd21207    81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVAD 93
Cdd:cd21207     1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                          90       100
                  ....*....|....*....|....*..
gi 1339089646  94 VDVFQTVDLWEKKDIAQVTTTLFALGR 120
Cdd:cd21207    81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  17 LEQEKEAQAWIETVLGEKFP--PGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENIN-MFQQALKNYGVAD 93
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPgvRVTNFTTDLRDGLALCALLNKLAPGLVDKKKLNKSEFDKLENINlALDVAEKKLGVPK 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1339089646  94 VDVfQTVDLWEKKDIaQVTTTLFALGRTTYR 124
Cdd:pfam00307  81 VLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 1339089646 157 IGLQAGQNKGATQAGQN-LGAGRKI 180
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTaFGAVRHI 25

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVAD 93
Cdd:cd21207     1 KRDPALEAEALDWIEAVTGEKLDDGKDYEDVLKDGVILCKLINILKPGSVKKINTSKMAFKLMENIENFLTACKGYGVPK 80

                          90       100
                  ....*....|....*....|....*..
gi 1339089646  94 VDVFQTVDLWEKKDIAQVTTTLFALGR 120
Cdd:cd21207    81 TDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain found in Saccharomyces cerevisiae transgelin (SCP1) and similar proteins; The family includes transgelins from Saccharomyces cerevisiae and Schizosaccharomyces pombe, which are also called SCP1 and STG1, respectively. Transgelin, also called calponin homolog 1, has actin-binding and actin-bundling activity. It stabilizes actin filaments against disassembly. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  19 QEKEAQAWIETVLGEKFPPGEaFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVADVDVFQ 98
Cdd:cd21210     1 AEQEAREWIEEVLGEKLAQGD-LLDALKDGVVLCKLANRILPADIRKYKESKMPFVQMENISAFLNAARKLGVPENDLFQ 79

                          90       100
                  ....*....|....*....|..
gi 1339089646  99 TVDLWEKKDIAQVTTTLFALGR 120
Cdd:cd21210    80 TVDLFERKNPAQVLQCLHALSR 101

calponin homology (CH) domain found in the IQ motif containing GTPase activating protein family; Members of the IQ motif containing GTPase activating protein (IQGAP) family are associated with the Ras GTP-binding protein and act as essential regulators of cytoskeletal function. There are three known IQGAP family members: IQGAP1, IQGAP2, and IQGAP3. They are multi-domain molecules having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both IQGAP1 and IQGAP2 specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP3 regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  22 EAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSIS--KINTSGGQFKMMENINMFQQALKNYGVADVDVFQT 99
Cdd:cd21206    12 EAKQWIEACLNEELPPTTEFEEELRNGVVLAKLANKFAPKLVPlkKIYDVGLQFRHTDNINHFLRALKKIGLPKIFHFET 91

                          90
                  ....*....|....*....
gi 1339089646 100 VDLWEKKDIAQVTTTLFAL 118
Cdd:cd21206    92 TDLYEKKNIPKVIYCLHAL 110

calponin homology (CH) domain found in the transgelin family; The transgelin (TAGLN) family includes transgelin, transgelin-2 and transgelin-3. Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. Transgelin-2, also called epididymis tissue protein Li 7e, or SM22-alpha homolog, acts as an actin-binding protein that induces actin gelation and regulates actin cytoskeleton. It may participate in the development and progression of multiple cancers. Transgelin-3, also called neuronal protein 22 (NP22), or neuronal protein NP25, may have a role in alcohol-related adaptations and may mediate regulatory signal transduction pathways in neurons. Members of this family contain a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  16 DLEQEKEAQAWIETVLGE---KFPPGEA-FEDVLRDGQVLCKLMNKISP---GSISKINTSGGQFKMMENINMFQQALKN 88
Cdd:cd21209     1 DPELESRLVEWIVAQCGSdvgRPDPGRLgFQKWLKDGTVLCKLINSLYPegsKPVKKIQSSKMAFKQMEQISQFLKAAED 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1339089646  89 YGVADVDVFQTVDLWEKKDIAQVTTTLFALG 119
Cdd:cd21209    81 YGVRTTDIFQTVDLWEGKDMAAVQRTLMALG 111

calponin homology (CH) domain found in calponin-3; Calponin-3 (CNN3), also called acidic isoform calponin, is an F-actin-binding protein that is expressed in the brain and has been shown to control dendritic spine morphology, density, and plasticity by regulating actin cytoskeletal reorganization and dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGekFPPGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVAD 93
Cdd:cd21284     1 KYDPQKEEELRNWIEEVTG--MSIGENFQKGLKDGVILCELINKLQPGSIRKINESKLNWHQLENIGNFIKAIQAYGMKP 78

                          90       100
                  ....*....|....*....|....*
gi 1339089646  94 VDVFQTVDLWEKKDIAQVTTTLFAL 118
Cdd:cd21284    79 HDIFEANDLFENGNMTQVQTTLLAL 103

calponin homology (CH) domain found in transgelin-2; Transgelin-2, also called epididymis tissue protein Li 7e, or SM22-alpha homolog, acts as an actin-binding protein that induces actin gelation and regulates the actin cytoskeleton. It may participate in the development and progression of multiple cancers. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGEKFP---PG-EAFEDVLRDGQVLCKLMNKISPGS---ISKINTSGGQFKMMENINMFQQAL 86
Cdd:cd21280     4 QYDADLEQILIQWITAQCGKQVGrpqPGrENFQNWLKDGTVLCHLINSLYPKGqapVKKIQASTMAFKQMEQISQFLQAA 83

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1339089646  87 KNYGVADVDVFQTVDLWEKKDIAQVTTTLFALG 119
Cdd:cd21280    84 ERYGINTTDIFQTVDLWEGKNMASVQRTLMNLG 116

calponin homology (CH) domain found in calponin-2; Calponin-2 (CNN2), also called neutral calponin, or smooth muscle calponin H2, is an actin cytoskeleton-associated regulatory protein that inhibits the activity of myosin-ATPase and cytoskeleton dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  16 DLEQEKEAQAWIETVLGEKFppGEAFEDVLRDGQVLCKLMNKISPGSISKINTSGGQFKMMENINMFQQALKNYGVADVD 95
Cdd:cd21283     1 DPQKEAELRTWIEGLTGRSI--GPDFQKGLKDGVILCELMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGMKPVD 78

                          90       100
                  ....*....|....*....|...
gi 1339089646  96 VFQTVDLWEKKDIAQVTTTLFAL 118
Cdd:cd21283    79 LFEANDLFESGNMTQVQVSLLAL 101

Calponin homology domain; Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). These domains cross-link actin filaments into bundles and networks. A calponin homology domain is predicted in yeasst Cdc24p.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646   21 KEAQAWIETVLGE-KFPPGEAFEDVLRDGQVLCKLMNKISPGSISK--INTSGGQFKMMENINMFQQALKNYGVaDVDVF 97
Cdd:smart00033   1 KTLLRWVNSLLAEyDKPPVTNFSSDLKDGVALCALLNSLSPGLVDKkkVAASLSRFKKIENINLALSFAEKLGG-KVVLF 79

                           90       100
                   ....*....|....*....|..
gi 1339089646   98 QTVDLWE-KKDIAQVTTTLFAL 118
Cdd:smart00033  80 EPEDLVEgPKLILGVIWTLISL 101

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  16 DLEQEKEAQAWIETVLGEKFPPGE----AFEDVLRDGQVLCKLMNKISPGSISKI----NTSGGQFKMMENINMFQQALK 87
Cdd:cd21279     1 DEELEERLVEWIVVQCGPDVGRPDrgrlGFQVWLKNGVVLSKLVNSLYPDGSKPVkipdNPPSMVFKQMEQVAQFLKAAE 80

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1339089646  88 NYGVADVDVFQTVDLWEKKDIAQVTTTLFALG 119
Cdd:cd21279    81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALG 112

calponin homology (CH) domain found in Ras GTPase-activating-like protein IQGAP3; IQ motif containing GTPase activating protein 3 (IQGAP3) associates with Ras GTP-binding proteins. It regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton. It belongs to the IQGAP family, which consists of multi-domain proteins having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP3 contains a single copy of the CH domain at the N-terminus.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  21 KEAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSI--SKI--------NTSGGQFKMMENINMFQQALKNYG 90
Cdd:cd21276    11 EEAKRWMEACLKEELPPPTELEESLRNGVYLAKLGHCFAPRVVplKKIydleqmryQATGLHFRHTDNINHWRNAMMHIG 90

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1339089646  91 VADVDVFQTVDLWEKKDIAQVTTTLFALGRTTYRhpewpgpyLGPRPAEE---CKRDFTEEQL 150
Cdd:cd21276    91 LPSIFHPETTDIYDKKNMPRVVYCIHALSLYLFR--------LGLAPQIHdlyGKVKFTEEEI 145

first calponin homology (CH) domain found in the plastin/fimbrin family; This family includes plastin and fimbrin. Plastin has three isoforms, plastin-1, -2, and -3, which are all actin-bundling proteins. Plastin-1, also called intestine-specific plastin, or I-plastin, is an actin-bundling protein in the absence of calcium. Plastin-2, also called L-plastin, LC64P, or lymphocyte cytosolic protein 1 (LCP-1), plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. It modulates the cell surface expression of IL2RA/CD25 and CD69. Plastin-3, also called T-plastin, is found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It may play a role in the regulation of bone development. Fimbrin has been found in plants and fungi. Arabidopsis thaliana fimbrin (AtFIM) includes fimbrin-1, -2, -3, -4, and -5; they cross-link actin filaments (F-actin) in a calcium independent manner. They stabilize and prevent F-actin depolymerization mediated by profilin. They act as key regulators of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus. AtFIM5 is an actin bundling factor that is required for pollen germination and pollen tube growth. Fungal fimbrin binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. Members of this family contain four copies of the CH domain. This model corresponds to the first CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  20 EKEAQA-WIETVLGE---------KFPPGEAFEDVLRDGQVLCKLMNKISPGSI--SKINTSG--GQFKMMENINMFQQA 85
Cdd:cd21217     2 EKEAFVeHINSLLADdpdlkhllpIDPDGDDLFEALRDGVLLCKLINKIVPGTIdeRKLNKKKpkNIFEATENLNLALNA 81

                          90       100
                  ....*....|....*....|....*.
gi 1339089646  86 LKNYGVadvdvfQTV-----DLWEKK 106
Cdd:cd21217    82 AKKIGC------KVVnigpqDILDGN 101

calponin homology (CH) domain found in Ras GTPase-activating-like protein IQGAP1; IQ motif containing GTPase activating protein 1 (IQGAP1), also called p195, is a homodimeric protein that is widely expressed among vertebrate cell types from early embryogenesis. It plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. It belongs to the IQGAP family, which consists of multi-domain proteins having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both, IQGAP1 and IQGAP2, specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP1 contains a single copy of the CH domain at the N-terminus.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  21 KEAQAWIETVLGEKFPPGEAFEDVLRDGQVLCKLMNKISPGSIS----------KINTSGGQFKMMENINMFQQALKNYG 90
Cdd:cd21274    12 EEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSlkkiydreqtRYKATGLHFRHTDNVIQWLNAMDEIG 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1339089646  91 VADVDVFQTVDLWEKKDIAQVTTTLFALGRTTYRhpewpgpyLGPRPAEE---CKRDFTEEQL 150
Cdd:cd21274    92 LPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFK--------LGLAPQIQdlyGKVDFTEEEI 146

calponin homology (CH) domain found in the growth arrest-specific protein 2 family; The growth arrest-specific protein 2 (GAS-2) family includes GAS-2, and GAS-2 like proteins, GAS2L1-3. GAS-2 may play a role in apoptosis by acting as a cell death substrate for caspases. GAS2L1 (also called GAS2-related protein on chromosome 22 or growth arrest-specific protein 2-like 1) and GAS2L2 (also called GAS2-related protein on chromosome 17 or growth arrest-specific protein 2-like 2) may be involved in the cross-linking of microtubules and microfilaments. GAS2L3, also called GAS2-like protein 3, is a cytoskeletal linker protein that may promote and stabilize the formation of the actin and microtubule network. Members of this family contain a single copy of the CH domain at the N-terminal region. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  26 WIETVLGEKFPPgEAFEDVLRDGQVLCKLMNKI--------------SPGSISKI----NTSGGQFKMMENINMFQQALK 87
Cdd:cd21204    14 WLNDLLGDDLTP-DNFLDELRNGVVLCQLAQKIqeaaekareagkknGPPPSYKLkcneNAKPGSFFARDNVANFLRWCR 92

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1339089646  88 NYGVADVDVFQTVDLWEKKDIAQVTTTLFALGRTTYR 124
Cdd:cd21204    93 KLGVDEVLLFESEDLVLHKNPRQVLLCLLELARIAAR 129

calponin homology (CH) domain found in the Pak Interactive eXchange factor family; Pak Interactive eXchange factor (PIX) proteins are Rho guanine nucleotide exchange factors (GEFs), which activate small GTPases by exchanging bound GDP for free GTP. They act as GEFs for both Cdc42 and Rac1, and have been implicated in cell motility, adhesion, neurite outgrowth, and cell polarity. Vertebrates contain two proteins from the PIX family, alpha-PIX and beta-PIX. Alpha-PIX, also called Rho guanine nucleotide exchange factor 6 (ARHGEF6), is localized in dendritic spines where it regulates spine morphogenesis. It controls dendritic length and spine density in the hippocampus. Mutations in the ARHGEF6 gene cause X-linked intellectual disability in humans. Beta-PIX, also called Rho guanine nucleotide exchange factor 7 (ARHGEF7), plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Both alpha-PIX and beta-PIX contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  37 PGEAFEDVLRDGQVLCKLMNKISPGSISKI-NTSGGQFKMMENINMFQQALKNYGVADVDVFQTVDLWEKKDIAQVTTTL 115
Cdd:cd21202    26 PERFLSESLKNGVVLCRLVNRLKPGTVEKIyDEPTTEEECLYNFESFLKACQELGILAEEIFDPNDLYSGGNFQKVLSTL 105

                  ....*..
gi 1339089646 116 FALGRTT 122
Cdd:cd21202   106 ERLEKVA 112

calponin homology (CH) domain found in VAV3 protein and similar proteins; VAV3 is ubiquitously expressed and functions as a phosphorylation-dependent guanine nucleotide exchange factor (GEF) for RhoA, RhoG, and Rac1. Its function has been implicated in the hematopoietic, bone, cerebellar, and cardiovascular systems. VAV3 is essential in axon guidance in neurons that control blood pressure and respiration. It is overexpressed in prostate cancer cells and it plays a role in regulating androgen receptor transcriptional activity. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. The model corresponds to CH domain, an actin-binding domain which is present as a single copy in VAV3 protein.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  45 LRDGQVLCKLMNKISPGSIS--KIN--TSGGQFKMMENINMFQQA-LKNYGVADVDVFQTVDLWEKKDIAQVTTTLFALG 119
Cdd:cd21264    36 LRDGVLLCQLLNNLRPHSINlkEINlrPQMSQFLCLKNIRTFLSAcCETFGMRKSELFEAFDLFDVRDFGKVIETLSKLS 115

                  ..
gi 1339089646 120 RT 121
Cdd:cd21264   116 RT 117

calponin homology (CH) domain found in beta-Pak Interactive eXchange factor; Beta-Pak Interactive eXchange factor (beta-PIX), also called PAK-interacting exchange factor beta, Rho guanine nucleotide exchange factor 7 (ARHGEF7), p85, or Cool (Cloned out of Library)-1, activates small GTPases by exchanging bound GDP for free GTP. It acts as a GEF for both Cdc42 and Rac1, and plays important roles in regulating neuroendocrine exocytosis, focal adhesion maturation, cell migration, synaptic vesicle localization, and insulin secretion. Beta-PIX contains a single copy of the CH domain at its N-terminus. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1339089646  45 LRDGQVLCKLMNKISPGSISKI-NTSGGQFKMMENINMFqqaLKNYGVADVDVFQTVDLWEKKDIAQVTTTLFALGRTT 122
Cdd:cd21266    33 LKDGVVLCRLLERLLPGSIDKVyPEPRTESECLSNIREF---LRGCGALRLETFDANDLYQGQNFNKVLSSLVALNKVT 108

third calponin homology (CH) domain found in Saccharomyces cerevisiae fimbrin and similar proteins; Fimbrin binds to actin, and functionally associates with actin structures involved in the development and maintenance of cell polarity. Members of this family contain four copies of the CH domain. This model corresponds to the third CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1339089646  16 DLEQEKEAQA---WIETvLGEKFPPGEAFEDvLRDGQVLCKLMNKISPGSIS--KINTSG-----GQFKMMENIN 80
Cdd:cd21300     2 DAEGEREARVftlWLNS-LDVEPAVNDLFED-LRDGLILLQAYDKVIPGSVNwkKVNKAPasaeiSRFKAVENTN 74

calponin homology (CH) domain found in VAV2 protein and similar proteins; VAV2 is widely expressed and functions as a guanine nucleotide exchange factor (GEF) for RhoA, RhoB and RhoG and also activates Rac1 and Cdc42. It is implicated in many cellular and physiological functions including blood pressure control, eye development, neurite outgrowth and branching, EGFR endocytosis and degradation, and cell cluster morphology, among others. It has been reported to associate with Nek3. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. The model corresponds to CH domain, an actin-binding domain which is present as a single copy in VAV2 protein.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  45 LRDGQVLCKLMNKISPGSIS----KINTSGGQFKMMENINMFQQALKN-YGVADVDVFQTVDLWEKKDIAQVTTtlfALG 119
Cdd:cd21263    36 LRDGVLLCQLLHNLSPGSIDlkdiNFRPQMSQFLCLKNIRTFLKVCHDkFGLRNSELFDPFDLFDVRDFGKVIS---ALS 112

                  ....
gi 1339089646 120 RTTY 123
Cdd:cd21263   113 RLSH 116

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 1339089646 157 IGLQAGQNKGATQAGQN-LGAGRKI 180
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTaFGAVRHI 25

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 2; Leucine-rich repeat and calponin homology domain-containing protein 2 (LRCH2) may play a role in the organization of the cytoskeleton. It contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1339089646  14 KRDLEQEKEAQAWIETVLGEKFPpgEAFEDVLRDGQVLCKLMNKISPGSISKINTSG------GQFKMMENINMFQQALK 87
Cdd:cd21271     1 REELELIEQLRENIESRLKVILP--EDLGAALMDGVVLCHLANHIRPRSVGSIHVPSpavpklSMAKCRRNVENFLDACR 78

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1339089646  88 NYGVADVDVFQTVDLWEKKDIAQVTTTLFAL 118
Cdd:cd21271    79 KLGVPEDKLCLPHHILEEKGLVKVSVTVQAL 109

third calponin homology (CH) domain found in the Arabidopsis thaliana fimbrin family; The Arabidopsis thaliana fimbrin (AtFIM) family includes Fimbrin-1, -2, -3, -4, and -5, which cross-link actin filaments (F-actin) in a calcium independent manner. They stabilize and prevent F-actin depolymerization mediated by profilin. They act as key regulators of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus. AtFIM5 is an actin bundling factor that is required for pollen germination and pollen tube growth. Members of this family contain four copies of the CH domain. This model corresponds to the third CH domain. CH domains are actin filament (F-actin) binding motifs.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1339089646  16 DLEQEKEAQAWIETVLGEKFPpGEAFEDVlRDGQVLCKLMNKISPGSISKINTSGG----QFKMMENIN 80
Cdd:cd21299     2 TSREERCFRLWINSLGIDTYV-NNVFEDV-RDGWVLLEVLDKVSPGSVNWKHANKPpikmPFKKVENCN 68