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Conserved domains on  [gi|1958785984|ref|XP_017449960|]
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pleckstrin homology domain-containing family G member 3 isoform X6 [Rattus norvegicus]

Protein Classification

pleckstrin homology domain-containing family G protein( domain architecture ID 10457355)

pleckstrin homology (PH) domain-containing family G protein contains PH and RhoGEF domains and may function as a guanine nucleotide exchange factor; similar to Homo sapiens pleckstrin homology domain-containing family G members 1/2/3 (PLEKHG1/2/3) that are involved in the regulation of Rho protein signal transduction

Gene Ontology:  GO:0005085|GO:0051056
PubMed:  11738596

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PH_PLEKHG1_G2_G3 cd13243
Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) ...
274-417 1.87e-67

Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) domain; PLEKHG1 (also called ARHGEF41), PLEKHG2 (also called ARHGEF42 or CLG/common-site lymphoma/leukemia guanine nucleotide exchange factor2), and PLEKHG3 (also called ARHGEF43) have RhoGEF DH/double-homology domains in tandem with a PH domain which is involved in phospholipid binding. They function as a guanine nucleotide exchange factor (GEF) and are involved in the regulation of Rho protein signal transduction. Mutations in PLEKHG1 have been associated panic disorder (PD), an anxiety disorder characterized by panic attacks and anticipatory anxiety. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


:

Pssm-ID: 270063 [Multi-domain]  Cd Length: 147  Bit Score: 223.77  E-value: 1.87e-67
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  274 EVVEDAIDTMTCVAWYINDMKRRHEHAVRLQEIQSLLINWKGPDLTTYGELVLEATFRVHRVRNERTFFLFDKILLITKK 353
Cdd:cd13243      2 SVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERLLFLFDKMLLITKK 81
                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958785984  354 RGDH-FVYKGHIPCSSLMLIES-TRDSLCFTVTHYKHSKQQYSIQAKTVEEKRSWTHHIKRLILEN 417
Cdd:cd13243     82 REDGiLQYKTHIMCSNLMLSESiPKEPLSFQVLPFDNPKLQYTLQAKNQEQKRLWTQEIKRLILEN 147
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
118-291 1.89e-51

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


:

Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 179.03  E-value: 1.89e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  118 VVREIVETERMYVQDLRSIVEDYLlKIIDTPGLLKPEQVSALFGNIESIYALNSQ-LLRDLDSCNSDPVAVASCFVERSQ 196
Cdd:pfam00621    1 VIKELLQTERSYVRDLEILVEVFL-PPNSKPLSESEEEIKTIFSNIEEIYELHRQlLLEELLKEWISIQRIGDIFLKFAP 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  197 EFDIYTQYCNNYPNSVAALTECMQ-DKQQAKFFRDRQELLQ-HSLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDGFE 274
Cdd:pfam00621   80 GFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDYE 159
                          170
                   ....*....|....*..
gi 1958785984  275 VVEDAIDTMTCVAWYIN 291
Cdd:pfam00621  160 DLKKALEAIKEVAKQIN 176
 
Name Accession Description Interval E-value
PH_PLEKHG1_G2_G3 cd13243
Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) ...
274-417 1.87e-67

Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) domain; PLEKHG1 (also called ARHGEF41), PLEKHG2 (also called ARHGEF42 or CLG/common-site lymphoma/leukemia guanine nucleotide exchange factor2), and PLEKHG3 (also called ARHGEF43) have RhoGEF DH/double-homology domains in tandem with a PH domain which is involved in phospholipid binding. They function as a guanine nucleotide exchange factor (GEF) and are involved in the regulation of Rho protein signal transduction. Mutations in PLEKHG1 have been associated panic disorder (PD), an anxiety disorder characterized by panic attacks and anticipatory anxiety. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270063 [Multi-domain]  Cd Length: 147  Bit Score: 223.77  E-value: 1.87e-67
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  274 EVVEDAIDTMTCVAWYINDMKRRHEHAVRLQEIQSLLINWKGPDLTTYGELVLEATFRVHRVRNERTFFLFDKILLITKK 353
Cdd:cd13243      2 SVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERLLFLFDKMLLITKK 81
                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958785984  354 RGDH-FVYKGHIPCSSLMLIES-TRDSLCFTVTHYKHSKQQYSIQAKTVEEKRSWTHHIKRLILEN 417
Cdd:cd13243     82 REDGiLQYKTHIMCSNLMLSESiPKEPLSFQVLPFDNPKLQYTLQAKNQEQKRLWTQEIKRLILEN 147
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
118-291 1.89e-51

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 179.03  E-value: 1.89e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  118 VVREIVETERMYVQDLRSIVEDYLlKIIDTPGLLKPEQVSALFGNIESIYALNSQ-LLRDLDSCNSDPVAVASCFVERSQ 196
Cdd:pfam00621    1 VIKELLQTERSYVRDLEILVEVFL-PPNSKPLSESEEEIKTIFSNIEEIYELHRQlLLEELLKEWISIQRIGDIFLKFAP 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  197 EFDIYTQYCNNYPNSVAALTECMQ-DKQQAKFFRDRQELLQ-HSLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDGFE 274
Cdd:pfam00621   80 GFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDYE 159
                          170
                   ....*....|....*..
gi 1958785984  275 VVEDAIDTMTCVAWYIN 291
Cdd:pfam00621  160 DLKKALEAIKEVAKQIN 176
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
118-292 4.76e-49

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 172.49  E-value: 4.76e-49
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   118 VVREIVETERMYVQDLRSIVEDYLLKIIDTPGLLKPEQVSALFGNIESIYALNSQLLRDLDSC----NSDPVAVASCFVE 193
Cdd:smart00325    1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKLLSPNELETLFGNIEEIYEFHRDFLDELEERieewDDSVERIGDVFLK 80
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   194 RSQEFDIYTQYCNNYPNSVAALTECMQDKQQAKFFRDRQELLQH-SLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDG 272
Cdd:smart00325   81 LEEFFKIYSEYCSNHPDALELLKKLKKNPRFQKFLKEIESSPQCrRLTLESLLLKPVQRLTKYPLLLKELLKHTPEDHED 160
                           170       180
                    ....*....|....*....|
gi 1958785984   273 FEVVEDAIDTMTCVAWYIND 292
Cdd:smart00325  161 REDLKKALKAIKELANQVNE 180
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
117-291 1.75e-46

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 165.16  E-value: 1.75e-46
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  117 RVVREIVETERMYVQDLRSIVEDYLLKIIDTPGLLKPEQVSALFGNIESIYALNSQLLRDLDSC----NSDPVAVASCFV 192
Cdd:cd00160      3 EVIKELLQTERNYVRDLKLLVEVFLKPLDKELLPLSPEEVELLFGNIEEIYEFHRIFLKSLEERveewDKSGPRIGDVFL 82
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  193 ERSQEFDIYTQYCNNYPNSVAALTECMQDKQQAKFFRDRQELLQHSLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDG 272
Cdd:cd00160     83 KLAPFFKIYSEYCSNHPDALELLKKLKKFNKFFQEFLEKAESECGRLKLESLLLKPVQRLTKYPLLLKELLKHTPDGHED 162
                          170
                   ....*....|....*....
gi 1958785984  273 FEVVEDAIDTMTCVAWYIN 291
Cdd:cd00160    163 REDLKKALEAIKEVASQVN 181
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
336-414 7.88e-09

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 54.48  E-value: 7.88e-09
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   336 RNERTFFLFDKILLITKKRGDH--FVYKGHIPCSSLMLIESTRDSL-----CFTVTHykHSKQQYSIQAKTVEEKRSWTH 408
Cdd:smart00233   18 WKKRYFVLFNSTLLYYKSKKDKksYKPKGSIDLSGCTVREAPDPDSskkphCFEIKT--SDRKTLLLQAESEEEREKWVE 95

                    ....*.
gi 1958785984   409 HIKRLI 414
Cdd:smart00233   96 ALRKAI 101
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
118-371 1.75e-08

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 59.13  E-value: 1.75e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  118 VVREIVETERMYVQDLrSIVEDYLLKIIDT----PGLLKPEQVSALFGNIESIYALNSQLLRDL-DSCNSDPVA--VASC 190
Cdd:COG5422    488 AIYEVIYTERDFVKDL-EYLRDTWIKPLEEsniiPENARRNFIKHVFANINEIYAVNSKLLKALtNRQCLSPIVngIADI 566
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  191 FVERSQEFDIYTQYCNNYPNSVAALTEcmQDKQQAKFFR--DRQELLQHSLPLG--SYLLKPVQRILKYHLLLQEIAKHF 266
Cdd:COG5422    567 FLDYVPKFEPFIKYGASQPYAKYEFER--EKSVNPNFARfdHEVERLDESRKLEldGYLTKPTTRLARYPLLLEEVLKFT 644
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  267 DEEEDGFEVVEDAIDT----MTCVAWYINDMKRRHEHavrLQEIQSLLINWKGPDLT---TYGELVLEATFRVH------ 333
Cdd:COG5422    645 DPDNPDTEDIPKVIDMlrefLSRLNFESGKAENRGDL---FHLNQQLLFKPEYVNLGlndEYRKIIFKGVLKRKaksktd 721
                          250       260       270       280
                   ....*....|....*....|....*....|....*....|....
gi 1958785984  334 -RVRNERTFFLFDKILLITK-----KRGDHFVYKGHIPCSSLML 371
Cdd:COG5422    722 gSLRGDIQFFLLDNMLLFCKakavnKWRQHKVFQRPIPLELLFI 765
PH pfam00169
PH domain; PH stands for pleckstrin homology.
338-414 1.98e-06

PH domain; PH stands for pleckstrin homology.


Pssm-ID: 459697 [Multi-domain]  Cd Length: 105  Bit Score: 47.56  E-value: 1.98e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  338 ERTFFLFDKILLITK--KRGDHFVYKGHIPCSSLMLIESTRDS-----LCFTV-THYKHSKQQYSIQAKTVEEKRSWTHH 409
Cdd:pfam00169   20 KRYFVLFDGSLLYYKddKSGKSKEPKGSISLSGCEVVEVVASDspkrkFCFELrTGERTGKRTYLLQAESEEERKDWIKA 99

                   ....*
gi 1958785984  410 IKRLI 414
Cdd:pfam00169  100 IQSAI 104
 
Name Accession Description Interval E-value
PH_PLEKHG1_G2_G3 cd13243
Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) ...
274-417 1.87e-67

Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) domain; PLEKHG1 (also called ARHGEF41), PLEKHG2 (also called ARHGEF42 or CLG/common-site lymphoma/leukemia guanine nucleotide exchange factor2), and PLEKHG3 (also called ARHGEF43) have RhoGEF DH/double-homology domains in tandem with a PH domain which is involved in phospholipid binding. They function as a guanine nucleotide exchange factor (GEF) and are involved in the regulation of Rho protein signal transduction. Mutations in PLEKHG1 have been associated panic disorder (PD), an anxiety disorder characterized by panic attacks and anticipatory anxiety. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270063 [Multi-domain]  Cd Length: 147  Bit Score: 223.77  E-value: 1.87e-67
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  274 EVVEDAIDTMTCVAWYINDMKRRHEHAVRLQEIQSLLINWKGPDLTTYGELVLEATFRVHRVRNERTFFLFDKILLITKK 353
Cdd:cd13243      2 SVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERLLFLFDKMLLITKK 81
                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1958785984  354 RGDH-FVYKGHIPCSSLMLIES-TRDSLCFTVTHYKHSKQQYSIQAKTVEEKRSWTHHIKRLILEN 417
Cdd:cd13243     82 REDGiLQYKTHIMCSNLMLSESiPKEPLSFQVLPFDNPKLQYTLQAKNQEQKRLWTQEIKRLILEN 147
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
118-291 1.89e-51

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 179.03  E-value: 1.89e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  118 VVREIVETERMYVQDLRSIVEDYLlKIIDTPGLLKPEQVSALFGNIESIYALNSQ-LLRDLDSCNSDPVAVASCFVERSQ 196
Cdd:pfam00621    1 VIKELLQTERSYVRDLEILVEVFL-PPNSKPLSESEEEIKTIFSNIEEIYELHRQlLLEELLKEWISIQRIGDIFLKFAP 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  197 EFDIYTQYCNNYPNSVAALTECMQ-DKQQAKFFRDRQELLQ-HSLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDGFE 274
Cdd:pfam00621   80 GFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDYE 159
                          170
                   ....*....|....*..
gi 1958785984  275 VVEDAIDTMTCVAWYIN 291
Cdd:pfam00621  160 DLKKALEAIKEVAKQIN 176
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
118-292 4.76e-49

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 172.49  E-value: 4.76e-49
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   118 VVREIVETERMYVQDLRSIVEDYLLKIIDTPGLLKPEQVSALFGNIESIYALNSQLLRDLDSC----NSDPVAVASCFVE 193
Cdd:smart00325    1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKLLSPNELETLFGNIEEIYEFHRDFLDELEERieewDDSVERIGDVFLK 80
                            90       100       110       120       130       140       150       160
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   194 RSQEFDIYTQYCNNYPNSVAALTECMQDKQQAKFFRDRQELLQH-SLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDG 272
Cdd:smart00325   81 LEEFFKIYSEYCSNHPDALELLKKLKKNPRFQKFLKEIESSPQCrRLTLESLLLKPVQRLTKYPLLLKELLKHTPEDHED 160
                           170       180
                    ....*....|....*....|
gi 1958785984   273 FEVVEDAIDTMTCVAWYIND 292
Cdd:smart00325  161 REDLKKALKAIKELANQVNE 180
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
117-291 1.75e-46

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 165.16  E-value: 1.75e-46
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  117 RVVREIVETERMYVQDLRSIVEDYLLKIIDTPGLLKPEQVSALFGNIESIYALNSQLLRDLDSC----NSDPVAVASCFV 192
Cdd:cd00160      3 EVIKELLQTERNYVRDLKLLVEVFLKPLDKELLPLSPEEVELLFGNIEEIYEFHRIFLKSLEERveewDKSGPRIGDVFL 82
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  193 ERSQEFDIYTQYCNNYPNSVAALTECMQDKQQAKFFRDRQELLQHSLPLGSYLLKPVQRILKYHLLLQEIAKHFDEEEDG 272
Cdd:cd00160     83 KLAPFFKIYSEYCSNHPDALELLKKLKKFNKFFQEFLEKAESECGRLKLESLLLKPVQRLTKYPLLLKELLKHTPDGHED 162
                          170
                   ....*....|....*....
gi 1958785984  273 FEVVEDAIDTMTCVAWYIN 291
Cdd:cd00160    163 REDLKKALEAIKEVASQVN 181
PH_Collybistin_ASEF cd01224
Collybistin/APC-stimulated guanine nucleotide exchange factor pleckstrin homology (PH) domain; ...
298-406 3.48e-11

Collybistin/APC-stimulated guanine nucleotide exchange factor pleckstrin homology (PH) domain; Collybistin (also called PEM2) is homologous to the Dbl proteins ASEF (also called ARHGEF4/RhoGEF4) and SPATA13 (Spermatogenesis-associated protein 13; also called ASEF2). It activates CDC42 specifically and not any other Rho-family GTPases. Collybistin consists of an SH3 domain, followed by a RhoGEF/DH and PH domain. In Dbl proteins, the DH and PH domains catalyze the exchange of GDP for GTP in Rho GTPases, allowing them to signal to downstream effectors. It induces submembrane clustering of the receptor-associated peripheral membrane protein gephyrin, which is thought to form a scaffold underneath the postsynaptic membrane linking receptors to the cytoskeleton. It also acts as a tumor suppressor that links adenomatous polyposis coli (APC) protein, a negative regulator of the Wnt signaling pathway and promotes the phosphorylation and degradation of beta-catenin, to Cdc42. Autoinhibition of collybistin is accomplished by the binding of its SH3 domain with both the RhoGEF and PH domains to block access of Cdc42 to the GTPase-binding site. Inactivation promotes cancer progression. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 269931  Cd Length: 138  Bit Score: 62.28  E-value: 3.48e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  298 EHAVRLQEIQSLLINWKGPDLTTYGELVLEATfRVHRVR----NERTFFLFDKILLITKK---RGDHFVYKGHIPcSSLM 370
Cdd:cd01224      2 ENLEKLAAWQSTVEGWEGEDLSDRSSELIHSG-ELTKISagraQERTFFLFDHQLVYCKKdllRRKNYIYKGRID-TDNM 79
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*
gi 1958785984  371 LIESTRDSLCF----TVTH----YKHSKQQ-YSIQAKTVEEKRSW 406
Cdd:cd01224     80 EIEDLPDGKDDesgvTVKNawkiYNASKNKwYVLCAKSAEEKQRW 124
PH_puratrophin-1 cd13242
Puratrophin-1 pleckstrin homology (PH) domain; Puratrophin-1 (also called Purkinje cell ...
317-416 9.55e-10

Puratrophin-1 pleckstrin homology (PH) domain; Puratrophin-1 (also called Purkinje cell atrophy-associated protein 1 or PLEKHG4/Pleckstrin homology domain-containing family G member 4) contains a spectrin repeat, a RhoGEF (DH) domain, and a PH domain. It is thought to function in intracellular signaling and cytoskeleton dynamics at the Golgi. Puratrophin-1 is expressed in kidney, Leydig cells in the testis, epithelial cells in the prostate gland and Langerhans islet in the pancreas. A single nucleotide substitution in the puratrophin-1 gene were once thought to result in autosomal dominant cerebellar ataxia (ADCA), but now it has been demonstrated that this ataxia is a result of defects in the BEAN gene. Puratrophin contains a domain architecture similar to that of Dbl family members Dbs and Trio. Dbs is a guanine nucleotide exchange factor (GEF), which contains spectrin repeats, a RhoGEF (DH) domain and a PH domain. The Dbs PH domain participates in binding to both the Cdc42 and RhoA GTPases. Trio plays an essential role in regulating the actin cytoskeleton during axonal guidance and branching. Trio is a multidomain signaling protein that contains two RhoGEF(DH)-PH domains in tandem. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270062  Cd Length: 136  Bit Score: 58.07  E-value: 9.55e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  317 DLTTYGELVLEATFRV--HRVRNERTFFLFDKILLITKKRG-----DHFVYKGHIPCSSLMLIESTRDS-LCFTVTHYKH 388
Cdd:cd13242     22 NLKEQGQLLRQDEFLVwqGRKKCLRHVFLFEDLILFSKPKKtpggkDVYIYKHSIKTSDIGLTENVGDSgLKFEIWFRRR 101
                           90       100
                   ....*....|....*....|....*....
gi 1958785984  389 SKQQ-YSIQAKTVEEKRSWTHHIKRLILE 416
Cdd:cd13242    102 KARDtYILQATSPEIKQAWTSDIAKLLWK 130
PH cd00821
Pleckstrin homology (PH) domain; PH domains have diverse functions, but in general are ...
338-410 2.19e-09

Pleckstrin homology (PH) domain; PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275388 [Multi-domain]  Cd Length: 92  Bit Score: 55.63  E-value: 2.19e-09
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958785984  338 ERTFFLFDKILLITKKRGD-HFVYKGHIPCSSLMLIESTRDSL---CFTVTHYKHskQQYSIQAKTVEEKRSWTHHI 410
Cdd:cd00821     18 KRWFVLFEGVLLYYKSKKDsSYKPKGSIPLSGILEVEEVSPKErphCFELVTPDG--RTYYLQADSEEERQEWLKAL 92
PH_Dbs cd01227
DBL's big sister protein pleckstrin homology (PH) domain; Dbs (also called MCF2-transforming ...
318-417 3.44e-09

DBL's big sister protein pleckstrin homology (PH) domain; Dbs (also called MCF2-transforming sequence-like protein 2) is a guanine nucleotide exchange factor (GEF), which contains spectrin repeats, a rhoGEF (DH) domain and a PH domain. The Dbs PH domain participates in binding to both the Cdc42 and RhoA GTPases. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 269934 [Multi-domain]  Cd Length: 126  Bit Score: 56.44  E-value: 3.44e-09
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  318 LTTYGELVLEATFRV-------HRV------RNERTFFLFDKILLITKKRGDH-----FVYKGHIPCSSLMLIESTR-DS 378
Cdd:cd01227      9 LGDLGKLLMQGSFNVwtehkkgHTKklarfkPMQRHIFLYEKAVLFCKKRGENgeapsYSYKNSLNTTAVGLTENVKgDT 88
                           90       100       110
                   ....*....|....*....|....*....|....*....
gi 1958785984  379 LCFTVtHYKHSKQQYSIQAKTVEEKRSWTHHIKRLILEN 417
Cdd:cd01227     89 KKFEI-WLNGREEVFIIQAPTPEIKAAWVKAIRQVLLSQ 126
PH smart00233
Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The ...
336-414 7.88e-09

Pleckstrin homology domain; Domain commonly found in eukaryotic signalling proteins. The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. PH domains have been found to possess inserted domains (such as in PLC gamma, syntrophins) and to be inserted within other domains. Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids.


Pssm-ID: 214574 [Multi-domain]  Cd Length: 102  Bit Score: 54.48  E-value: 7.88e-09
                            10        20        30        40        50        60        70        80
                    ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984   336 RNERTFFLFDKILLITKKRGDH--FVYKGHIPCSSLMLIESTRDSL-----CFTVTHykHSKQQYSIQAKTVEEKRSWTH 408
Cdd:smart00233   18 WKKRYFVLFNSTLLYYKSKKDKksYKPKGSIDLSGCTVREAPDPDSskkphCFEIKT--SDRKTLLLQAESEEEREKWVE 95

                    ....*.
gi 1958785984   409 HIKRLI 414
Cdd:smart00233   96 ALRKAI 101
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
118-371 1.75e-08

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 59.13  E-value: 1.75e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  118 VVREIVETERMYVQDLrSIVEDYLLKIIDT----PGLLKPEQVSALFGNIESIYALNSQLLRDL-DSCNSDPVA--VASC 190
Cdd:COG5422    488 AIYEVIYTERDFVKDL-EYLRDTWIKPLEEsniiPENARRNFIKHVFANINEIYAVNSKLLKALtNRQCLSPIVngIADI 566
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  191 FVERSQEFDIYTQYCNNYPNSVAALTEcmQDKQQAKFFR--DRQELLQHSLPLG--SYLLKPVQRILKYHLLLQEIAKHF 266
Cdd:COG5422    567 FLDYVPKFEPFIKYGASQPYAKYEFER--EKSVNPNFARfdHEVERLDESRKLEldGYLTKPTTRLARYPLLLEEVLKFT 644
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  267 DEEEDGFEVVEDAIDT----MTCVAWYINDMKRRHEHavrLQEIQSLLINWKGPDLT---TYGELVLEATFRVH------ 333
Cdd:COG5422    645 DPDNPDTEDIPKVIDMlrefLSRLNFESGKAENRGDL---FHLNQQLLFKPEYVNLGlndEYRKIIFKGVLKRKaksktd 721
                          250       260       270       280
                   ....*....|....*....|....*....|....*....|....
gi 1958785984  334 -RVRNERTFFLFDKILLITK-----KRGDHFVYKGHIPCSSLML 371
Cdd:COG5422    722 gSLRGDIQFFLLDNMLLFCKakavnKWRQHKVFQRPIPLELLFI 765
PH_Scd1 cd13246
Shape and Conjugation Deficiency 1 Pleckstrin homology (PH) domain; Fission yeast Scd1 is an ...
294-352 1.11e-06

Shape and Conjugation Deficiency 1 Pleckstrin homology (PH) domain; Fission yeast Scd1 is an exchange factor for Cdc42 and an effector of Ras1, the homolog of the human H-Ras. Scd2/Bem1 mediates Cdc42 activation by binding to Scd1/Cdc24 and to Cdc42. Ras1 regulates Scd1/Cdc24/Ral1, which is a putative guanine nucleotide exchange factor for Cdc42, a member of the Rho family of Ras-like proteins. Cdc42 then activates the Shk1/Orb2 protein kinase. Scd1 interacts with Klp5 and Klp6 kinesins to mediate cytokinesis. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270066  Cd Length: 148  Bit Score: 49.55  E-value: 1.11e-06
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1958785984  294 KRRHEHAVRLQEIQSLLINWKGPDLTTYGELVLEATFRVHRVRNERTF--FLFDKILLITK 352
Cdd:cd13246      1 QRRAENQQVVDDLKARVEDWKGHSLDSFGELLLHDTFTVRKDDSEREYhvYLFERILLCCK 61
PH pfam00169
PH domain; PH stands for pleckstrin homology.
338-414 1.98e-06

PH domain; PH stands for pleckstrin homology.


Pssm-ID: 459697 [Multi-domain]  Cd Length: 105  Bit Score: 47.56  E-value: 1.98e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  338 ERTFFLFDKILLITK--KRGDHFVYKGHIPCSSLMLIESTRDS-----LCFTV-THYKHSKQQYSIQAKTVEEKRSWTHH 409
Cdd:pfam00169   20 KRYFVLFDGSLLYYKddKSGKSKEPKGSISLSGCEVVEVVASDspkrkFCFELrTGERTGKRTYLLQAESEEERKDWIKA 99

                   ....*
gi 1958785984  410 IKRLI 414
Cdd:pfam00169  100 IQSAI 104
PH_Vav cd01223
Vav pleckstrin homology (PH) domain; Vav acts as a guanosine nucleotide exchange factor (GEF) ...
307-406 3.15e-06

Vav pleckstrin homology (PH) domain; Vav acts as a guanosine nucleotide exchange factor (GEF) for Rho/Rac proteins. They control processes including T cell activation, phagocytosis, and migration of cells. The Vav subgroup of Dbl GEFs consists of three family members (Vav1, Vav2, and Vav3) in mammals. Vav1 is preferentially expressed in the hematopoietic system, while Vav2 and Vav3 are described by broader expression patterns. Mammalian Vav proteins consist of a calponin homology (CH) domain, an acidic region, a catalytic Dbl homology (DH) domain, a PH domain, a zinc finger cysteine rich domain (C1/CRD), and an SH2 domain, flanked by two SH3 domains. In invertebrates such as Drosophila and C. elegans, Vav is missing the N-terminal SH3 domain. The DH domain is involved in RhoGTPase recognition and selectivity and stimulates the reorganization of the switch regions for GDP/GTP exchange. The PH domain is implicated in directing membrane localization, allosteric regulation of guanine nucleotide exchange activity, and as a phospholipid- dependent regulator of GEF activity. Vavs bind RhoGTPases including Rac1, RhoA, RhoG, and Cdc42, while other members of the GEF family are specific for a single RhoGTPase. This promiscuity is thought to be a result of its CRD. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but only a few (less than 10%) display strong specificity in binding inositol phosphates. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinases, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, cytoskeletal associated molecules, and in lipid associated enzymes.


Pssm-ID: 269930  Cd Length: 127  Bit Score: 47.63  E-value: 3.15e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  307 QSLLINWKGPDLTTYGELVLEATFRV-----HRVRNeRTFFLFDKILLITKK-RGDHFVYKGHIPCSS------LMLIES 374
Cdd:cd01223      2 IQDLIENLNESLADYGRLQIDGELKIkshedQKKKD-RYAFLFDKVLLICKSlRGDQYEYKEIINLSEyrieddPSRRTL 80
                           90       100       110
                   ....*....|....*....|....*....|....*
gi 1958785984  375 TRD---SLCFTVTHyKHSKQQYSIQAKTVEEKRSW 406
Cdd:cd01223     81 KRDkrwSYQFLLVH-KQGKTAYTLYAKTEELKKKW 114
PH2_Kalirin_Trio_p63RhoGEF cd13241
p63RhoGEF pleckstrin homology (PH) domain, repeat 2; The guanine nucleotide exchange factor ...
318-417 1.80e-05

p63RhoGEF pleckstrin homology (PH) domain, repeat 2; The guanine nucleotide exchange factor p63RhoGEF is an effector of the heterotrimeric G protein, Galphaq and linking Galphaq-coupled receptors (GPCRs) to the activation of RhoA. The Dbl(DH) and PH domains of p63RhoGEF interact with the effector-binding site and the C-terminal region of Galphaq and appear to relieve autoinhibition of the catalytic DH domain by the PH domain. Trio, Duet, and p63RhoGEF are shown to constitute a family of Galphaq effectors that appear to activate RhoA both in vitro and in intact cells. Dbs is a guanine nucleotide exchange factor (GEF), which contains spectrin repeats, a rhoGEF (DH) domain and a PH domain. The Dbs PH domain participates in binding to both the Cdc42 and RhoA GTPases. Trio plays an essential role in regulating the actin cytoskeleton during axonal guidance and branching. Trio is a multidomain signaling protein that contains two RhoGEF(DH)-PH domains in tandem. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270061  Cd Length: 140  Bit Score: 46.10  E-value: 1.80e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  318 LTTYGELVLEATFRVHRVRN-------ERTFFLFDKILLI-----TKKRGDH--FVYKGHIPCSSLMLIES-TRDSL--C 380
Cdd:cd13241     11 ITAQGKLLLQGTLLVSEPSAgllqkgkERRVFLFEQIIIFseilgKKTQFSNpgYIYKNHIKVNKMSLEENvDGDPLrfA 90
                           90       100       110
                   ....*....|....*....|....*....|....*..
gi 1958785984  381 FTVTHYKHSKQQYSIQAKTVEEKRSWTHHIKRlILEN 417
Cdd:cd13241     91 LKSRDPNNPSETFILQAASPEVRQEWVDTINQ-ILDT 126
PH1_FARP1-like cd01220
FERM, RhoGEF and pleckstrin domain-containing protein 1 and related proteins Pleckstrin ...
337-414 7.65e-04

FERM, RhoGEF and pleckstrin domain-containing protein 1 and related proteins Pleckstrin Homology (PH) domain, repeat 1; Members here include FARP1 (also called Chondrocyte-derived ezrin-like protein; PH domain-containing family C member 2), FARP2 (also called FIR/FERM domain including RhoGEF; FGD1-related Cdc42-GEF/FRG), and FARP6 (also called Zinc finger FYVE domain-containing protein 24). They are members of the Dbl family guanine nucleotide exchange factors (GEFs) which are upstream positive regulators of Rho GTPases. Little is known about FARP1 and FARP6, though FARP1 has increased expression in differentiated chondrocytes. FARP2 is thought to regulate neurite remodeling by mediating the signaling pathways from membrane proteins to Rac. It is found in brain, lung, and testis, as well as embryonic hippocampal and cortical neurons. FARP1 and FARP2 are composed of a N-terminal FERM domain, a proline-rich (PR) domain, Dbl-homology (DH), and two C-terminal PH domains. FARP6 is composed of Dbl-homology (DH), and two C-terminal PH domains separated by a FYVE domain. This hierarchy contains the first PH repeat. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 269928  Cd Length: 109  Bit Score: 40.38  E-value: 7.65e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958785984  337 NERTFFLFDKILLITKKRGD---HFVYKGHIPCSSLMlIESTRDSL----CFTVThykHSKQQYSIQAKTVEEKRSWTHH 409
Cdd:cd01220     23 QQRMFFLFSDVLLYTSRSPTpslQFKVHGQLPLRGLM-VEESEPEWgvahCFTIY---GGNRALTVAASSEEEKERWLED 98

                   ....*
gi 1958785984  410 IKRLI 414
Cdd:cd01220     99 LQRAI 103
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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