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Conserved domains on  [gi|966959103|ref|XP_015001107|]
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sorting nexin-16 isoform X1 [Macaca mulatta]

Protein Classification

PX domain-containing protein; PX and BAR domain-containing protein( domain architecture ID 10163585)

PX (Phox Homology) domain-containing protein may bind phosphoinositides and may function in targeting proteins to membranes| PX (Phox homology) and BAR (Bin/Amphiphysin/Rvs) domain-containing protein similar to Saccoglossus kowalevskii sorting nexin 2

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PX_SNX16 cd07276
The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a ...
105-214 4.30e-75

The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX16 contains a central PX domain followed by a coiled-coil region. SNX16 is localized in early and recycling endosomes through the binding of its PX domain to phosphatidylinositol-3-phosphate (PI3P). It plays a role in epidermal growth factor (EGF) signaling by regulating EGF receptor membrane trafficking.


:

Pssm-ID: 132809  Cd Length: 110  Bit Score: 226.91  E-value: 4.30e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 105 DRPSTPTILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDR 184
Cdd:cd07276    1 DRPIRPPILGYEVMEERARFTVYKIRVENKVGDSWFVFRRYTDFVRLNDKLKQMFPGFRLSLPPKRWFKDNFDPDFLEER 80
                         90       100       110
                 ....*....|....*....|....*....|
gi 966959103 185 QLGLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd07276   81 QLGLQAFVNNIMAHKDIAKCKLVREFFCLD 110
Mplasa_alph_rch super family cl37461
helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of ...
231-336 2.73e-03

helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of Mycoplasma species. Members average 750 amino acids in length, including signal peptide. Sequences are predicted (Jpred 3) to be almost entirely alpha-helical. These sequences show strong periodicity (consistent with long alpha helical structures) and low complexity rich in D,E,N,Q, and K. Genes encoding these proteins are often found in tandem. The function is unknown.


The actual alignment was detected with superfamily member TIGR04523:

Pssm-ID: 275316 [Multi-domain]  Cd Length: 745  Bit Score: 39.62  E-value: 2.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  231 ETLEETNYRLQKELLEKQKEMESLKKLLSEKQLHIDTLENRIRTLSVEPEESLDVSETEGEQILKVKSSALEVDQDVLD- 309
Cdd:TIGR04523 352 TNSESENSEKQRELEEKQNEIEKLKKENQSYKQEIKNLESQINDLESKIQNQEKLNQQKDEQIKKLQQEKELLEKEIERl 431
                          90       100
                  ....*....|....*....|....*..
gi 966959103  310 EESRADNKPCLSFSEPENAVSEIEVAE 336
Cdd:TIGR04523 432 KETIIKNNSEIKDLTNQDSVKELIIKN 458
 
Name Accession Description Interval E-value
PX_SNX16 cd07276
The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a ...
105-214 4.30e-75

The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX16 contains a central PX domain followed by a coiled-coil region. SNX16 is localized in early and recycling endosomes through the binding of its PX domain to phosphatidylinositol-3-phosphate (PI3P). It plays a role in epidermal growth factor (EGF) signaling by regulating EGF receptor membrane trafficking.


Pssm-ID: 132809  Cd Length: 110  Bit Score: 226.91  E-value: 4.30e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 105 DRPSTPTILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDR 184
Cdd:cd07276    1 DRPIRPPILGYEVMEERARFTVYKIRVENKVGDSWFVFRRYTDFVRLNDKLKQMFPGFRLSLPPKRWFKDNFDPDFLEER 80
                         90       100       110
                 ....*....|....*....|....*....|
gi 966959103 185 QLGLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd07276   81 QLGLQAFVNNIMAHKDIAKCKLVREFFCLD 110
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
136-211 2.34e-19

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 81.13  E-value: 2.34e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 966959103  136 EESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFkDNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:pfam00787   6 LEEWSVRRRYSDFVELHKKLLRKFPSVIIpPLPPKRWL-GRYNEEFIEKRRKGLEQYLQRLLQHPELRNSEVLLEFL 81
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
115-211 4.43e-18

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 78.54  E-value: 4.43e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103   115 YEVMEERAKFTVYKIlVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWF--KDNYNADFLEDRQLGLQAF 191
Cdd:smart00312   5 EKIGDGKHYYYVIEI-ETKTGLEEWTVSRRYSDFLELHSKLKKHFPRSILpPLPGKKLFgrLNNFSEEFIEKRRRGLEKY 83
                           90       100
                   ....*....|....*....|.
gi 966959103   192 LQNLVAHKDIAN-CLAVREFL 211
Cdd:smart00312  84 LQSLLNHPELINhSEVVLEFL 104
COG5391 COG5391
Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function ...
56-255 4.70e-07

Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function prediction only];


Pssm-ID: 227680 [Multi-domain]  Cd Length: 524  Bit Score: 51.34  E-value: 4.70e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  56 SVPDQMDNTSPVCSSPLIRTKFTGAASSIEYSTRPRETEEQNpetviwedrpstptilgyevMEERAKFTVYKILVKKT- 134
Cdd:COG5391  103 SLRDMLSLLLPTSLQPPLSTSHTILDYFISSTVSNPQSLTLL--------------------VDSRDKHTSYEIITVTNl 162
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 135 ------PEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRW----FKDNYNADFLEDRQLGLQAFLQNLVAHKDIAN 203
Cdd:COG5391  163 psfqlrESRPLVVRRRYSDFESLHSILIKLLPLCAIpPLPSKKSnseyYGDRFSDEFIEERRQSLQNFLRRVSTHPLLSN 242
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 966959103 204 CLAVReflclddppgpfDSLEESRAFCETLEETNYRLQKELLEKQKEMESLK 255
Cdd:COG5391  243 YKNSK------------SWESHSTLLSSFIENRKSVPTPLSLDLTSTTQELD 282
Mplasa_alph_rch TIGR04523
helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of ...
231-336 2.73e-03

helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of Mycoplasma species. Members average 750 amino acids in length, including signal peptide. Sequences are predicted (Jpred 3) to be almost entirely alpha-helical. These sequences show strong periodicity (consistent with long alpha helical structures) and low complexity rich in D,E,N,Q, and K. Genes encoding these proteins are often found in tandem. The function is unknown.


Pssm-ID: 275316 [Multi-domain]  Cd Length: 745  Bit Score: 39.62  E-value: 2.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  231 ETLEETNYRLQKELLEKQKEMESLKKLLSEKQLHIDTLENRIRTLSVEPEESLDVSETEGEQILKVKSSALEVDQDVLD- 309
Cdd:TIGR04523 352 TNSESENSEKQRELEEKQNEIEKLKKENQSYKQEIKNLESQINDLESKIQNQEKLNQQKDEQIKKLQQEKELLEKEIERl 431
                          90       100
                  ....*....|....*....|....*..
gi 966959103  310 EESRADNKPCLSFSEPENAVSEIEVAE 336
Cdd:TIGR04523 432 KETIIKNNSEIKDLTNQDSVKELIIKN 458
 
Name Accession Description Interval E-value
PX_SNX16 cd07276
The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a ...
105-214 4.30e-75

The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX16 contains a central PX domain followed by a coiled-coil region. SNX16 is localized in early and recycling endosomes through the binding of its PX domain to phosphatidylinositol-3-phosphate (PI3P). It plays a role in epidermal growth factor (EGF) signaling by regulating EGF receptor membrane trafficking.


Pssm-ID: 132809  Cd Length: 110  Bit Score: 226.91  E-value: 4.30e-75
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 105 DRPSTPTILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDR 184
Cdd:cd07276    1 DRPIRPPILGYEVMEERARFTVYKIRVENKVGDSWFVFRRYTDFVRLNDKLKQMFPGFRLSLPPKRWFKDNFDPDFLEER 80
                         90       100       110
                 ....*....|....*....|....*....|
gi 966959103 185 QLGLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd07276   81 QLGLQAFVNNIMAHKDIAKCKLVREFFCLD 110
PX_domain cd06093
The Phox Homology domain, a phosphoinositide binding module; The PX domain is a ...
111-213 9.88e-28

The Phox Homology domain, a phosphoinositide binding module; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to membranes. Proteins containing PX domains interact with PIs and have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. Many members of this superfamily bind phosphatidylinositol-3-phosphate (PI3P) but in some cases, other PIs such as PI4P or PI(3,4)P2, among others, are the preferred substrates. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction, as in the cases of p40phox, p47phox, and some sorting nexins (SNXs). The PX domain is conserved from yeast to humans and is found in more than 100 proteins. The majority of PX domain-containing proteins are SNXs, which play important roles in endosomal sorting.


Pssm-ID: 132768 [Multi-domain]  Cd Length: 106  Bit Score: 104.36  E-value: 9.88e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 111 TILGYEVMEERAK-FTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKdNYNADFLEDRQLGL 188
Cdd:cd06093    3 SIPDYEKVKDGGKkYVVYIIEVTTQGGEEWTVYRRYSDFEELHEKLKKKFPGVILpPLPPKKLFG-NLDPEFIEERRKQL 81
                         90       100
                 ....*....|....*....|....*
gi 966959103 189 QAFLQNLVAHKDIANCLAVREFLCL 213
Cdd:cd06093   82 EQYLQSLLNHPELRNSEELKEFLEL 106
PX_CISK cd06870
The phosphoinositide binding Phox Homology Domain of Cytokine-Independent Survival Kinase; The ...
107-214 9.75e-27

The phosphoinositide binding Phox Homology Domain of Cytokine-Independent Survival Kinase; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Cytokine-independent survival kinase (CISK), also called Serum- and Glucocorticoid-induced Kinase 3 (SGK3), plays a role in cell growth and survival. It is expressed in most tissues and is most abundant in the embryo and adult heart and spleen. It was originally discovered in a screen for antiapoptotic genes. It phosphorylates and inhibits the proapoptotic proteins, Bad and FKHRL1. CISK/SGK3 also regulates many transporters, ion channels, and receptors. It plays a critical role in hair follicle morphogenesis and hair cycling. N-terminal to a catalytic kinase domain, CISK contains a PX domain which binds highly phosphorylated PIs, directs membrane localization, and regulates the enzyme's activity.


Pssm-ID: 132780  Cd Length: 109  Bit Score: 101.72  E-value: 9.75e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 107 PSTPTILGYEVMEERAKFTVYKILVKkTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDRQL 186
Cdd:cd06870    3 PSVSIPSSDEDREKKKRFTVYKVVVS-VGRSSWFVFRRYAEFDKLYESLKKQFPASNLKIPGKRLFGNNFDPDFIKQRRA 81
                         90       100
                 ....*....|....*....|....*...
gi 966959103 187 GLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd06870   82 GLDEFIQRLVSDPKLLNHPDVRAFLQMD 109
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
136-211 2.34e-19

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 81.13  E-value: 2.34e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 966959103  136 EESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFkDNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:pfam00787   6 LEEWSVRRRYSDFVELHKKLLRKFPSVIIpPLPPKRWL-GRYNEEFIEKRRKGLEQYLQRLLQHPELRNSEVLLEFL 81
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
115-211 4.43e-18

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 78.54  E-value: 4.43e-18
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103   115 YEVMEERAKFTVYKIlVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWF--KDNYNADFLEDRQLGLQAF 191
Cdd:smart00312   5 EKIGDGKHYYYVIEI-ETKTGLEEWTVSRRYSDFLELHSKLKKHFPRSILpPLPGKKLFgrLNNFSEEFIEKRRRGLEKY 83
                           90       100
                   ....*....|....*....|.
gi 966959103   192 LQNLVAHKDIAN-CLAVREFL 211
Cdd:smart00312  84 LQSLLNHPELINhSEVVLEFL 104
PX_SNARE cd06897
The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi; The PX domain ...
119-211 2.19e-17

The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of fungal proteins similar to Saccharomyces cerevisiae Vam7p. They contain an N-terminal PX domain and a C-terminal SNARE domain. The SNARE (Soluble NSF attachment protein receptor) family of proteins are integral membrane proteins that serve as key factors for vesicular trafficking. Vam7p is anchored at the vacuolar membrane through the specific interaction of its PX domain with phosphatidylinositol-3-phosphate (PI3P) present in bilayers. It plays an essential role in vacuole fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction.


Pssm-ID: 132807  Cd Length: 108  Bit Score: 76.54  E-value: 2.19e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 119 EERAKFTVYKILVKkTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLA-LPPKRWFKDNY-NADFLEDRQLGLQAFLQNLV 196
Cdd:cd06897   10 VSPKPYTVYNIQVR-LPLRSYTVSRRYSEFVALHKQLESEVGIEPPYpLPPKSWFLSTSsNPKLVEERRVGLEAFLRALL 88
                         90
                 ....*....|....*..
gi 966959103 197 AHKDIA--NCLAVREFL 211
Cdd:cd06897   89 NDEDSRwrNSPAVKEFL 105
PX_YPT35 cd07280
The phosphoinositide binding Phox Homology domain of the fungal protein YPT35; The PX domain ...
122-211 7.36e-16

The phosphoinositide binding Phox Homology domain of the fungal protein YPT35; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of YPT35 proteins from the fungal subkingdom Dikarya. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of YPT35 binds to phosphatidylinositol 3-phosphate (PI3P). It also serves as a protein interaction domain, binding to members of the Yip1p protein family, which localize to the ER and Golgi. YPT35 is mainly associated with endosomes and together with Yip1p proteins, may be involved in a specific function in the endocytic pathway.


Pssm-ID: 132813  Cd Length: 120  Bit Score: 72.75  E-value: 7.36e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 122 AKFTVYKILVKKTPEE--SWVVFRRYTDFSRLNDKLKEMFPGFRLA----LPPKRWFKD---NYNADFLEDRQLGLQAFL 192
Cdd:cd07280   20 GAYVVWKITIETKDLIgsSIVAYKRYSEFVQLREALLDEFPRHKRNeipqLPPKVPWYDsrvNLNKAWLEKRRRGLQYFL 99
                         90
                 ....*....|....*....
gi 966959103 193 QNLVAHKDIANCLAVREFL 211
Cdd:cd07280  100 NCVLLNPVFGGSPVVKEFL 118
PX_MONaKA cd06871
The phosphoinositide binding Phox Homology domain of Modulator of Na,K-ATPase; The PX domain ...
125-216 1.74e-15

The phosphoinositide binding Phox Homology domain of Modulator of Na,K-ATPase; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. MONaKA (Modulator of Na,K-ATPase) binds the plasma membrane ion transporter, Na,K-ATPase, and modulates its enzymatic and ion pump activities. It modulates brain Na,K-ATPase and may be involved in regulating electrical excitability and synaptic transmission. MONaKA contains an N-terminal PX domain and a C-terminal catalytic kinase domain. The PX domain interacts with PIs and plays a role in targeting proteins to PI-enriched membranes.


Pssm-ID: 132781  Cd Length: 120  Bit Score: 71.62  E-value: 1.74e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 125 TVYKILVKK--TPEESWVVFRRYTDFSRLNDKLkeMFPGFRLALPPKRWFkDNYNADFLEDRQLGLQAFLQNLVAHKDIA 202
Cdd:cd06871   22 TEYIIRVQRgpSPENSWQVIRRYNDFDLLNASL--QISGISLPLPPKKLI-GNMDREFIAERQQGLQNYLNVILMNPILA 98
                         90
                 ....*....|....
gi 966959103 203 NCLAVREFLcldDP 216
Cdd:cd06871   99 SCLPVKKFL---DP 109
PX_SNX13 cd06873
The phosphoinositide binding Phox Homology domain of Sorting Nexin 13; The PX domain is a ...
117-212 2.68e-15

The phosphoinositide binding Phox Homology domain of Sorting Nexin 13; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX13, also called RGS-PX1, contains an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs. It specifically binds to the stimulatory subunit of the heterotrimeric G protein G(alpha)s, serving as its GTPase activating protein, through the RGS domain. It preferentially binds phosphatidylinositol-3-phosphate (PI3P) through the PX domain and is localized in early endosomes. SNX13 is involved in endosomal sorting of EGFR into multivesicular bodies (MVB) for delivery to the lysosome.


Pssm-ID: 132783  Cd Length: 120  Bit Score: 71.15  E-value: 2.68e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 117 VMEERAK-FTVYKILVKK----TPEESWVVFRRYTDFSRLNDKLKEMFPGFR-LALPPKRWFKdNYNADFLEDRQLGLQA 190
Cdd:cd06873   14 IVKEHGKtYAVYAISVTRiypnGQEESWHVYRRYSDFHDLHMRLKEKFPNLSkLSFPGKKTFN-NLDRAFLEKRRKMLNQ 92
                         90       100
                 ....*....|....*....|..
gi 966959103 191 FLQNLVAHKDIANCLAVREFLC 212
Cdd:cd06873   93 YLQSLLNPEVLDANPGLQEIVL 114
PX_SNX19_like_plant cd06872
The phosphoinositide binding Phox Homology domain of uncharacterized SNX19-like plant proteins; ...
112-211 5.11e-15

The phosphoinositide binding Phox Homology domain of uncharacterized SNX19-like plant proteins; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized plant proteins containing an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some sorting nexins (SNXs). This is the same domain architecture found in SNX19. SNX13 and SNX14 also contain these three domains but also contain a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction.


Pssm-ID: 132782  Cd Length: 107  Bit Score: 70.24  E-value: 5.11e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 112 ILGYEVMEERAK-FTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMfPGFRLALPPKRWFKDNYNADFLEDRQLGLQA 190
Cdd:cd06872    5 VLGAEIVKSGSKsFAVYSVAVTDNENETWVVKRRFRNFETLHRRLKEV-PKYNLELPPKRFLSSSLDGAFIEERCKLLDK 83
                         90       100
                 ....*....|....*....|.
gi 966959103 191 FLQNLVAHKDIANCLAVREFL 211
Cdd:cd06872   84 YLKDLLVIEKVAESHEVWSFL 104
PX_MDM1p cd06876
The phosphoinositide binding Phox Homology domain of yeast MDM1p; The PX domain is a ...
116-211 4.99e-12

The phosphoinositide binding Phox Homology domain of yeast MDM1p; The PX domain is a phosphoinositide binding (PI) module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Yeast MDM1p is a filament-like protein localized in punctate structures distributed throughout the cytoplasm. It plays an important role in nuclear and mitochondrial transmission to daughter buds. Members of this subfamily show similar domain architectures as some sorting nexins (SNXs). Some members are similar to SNX19 in that they contain an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some SNXs. Others are similar to SNX13 and SNX14, which also harbor these three domains as well as a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132786  Cd Length: 133  Bit Score: 62.33  E-value: 4.99e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 116 EVMEERAKFTVYKILVKK----TPEESWVVFRRYTDFSRLNDKLKEMFPGFR-LALPPKRWFKDNY-NADFLEDRQLGLQ 189
Cdd:cd06876   30 DVEEEGKEFVVYLIEVQRlnndDQSSGWVVARRYSEFLELHKYLKKRYPGVLkLDFPQKRKISLKYsKTLLVEERRKALE 109
                         90       100
                 ....*....|....*....|..
gi 966959103 190 AFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06876  110 KYLQELLKIPEVCEDEEFRKFL 131
PX_SNX1_2_like cd06859
The phosphoinositide binding Phox Homology domain of Sorting Nexins 1 and 2; The PX domain is ...
122-211 5.24e-12

The phosphoinositide binding Phox Homology domain of Sorting Nexins 1 and 2; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX1, SNX2, and similar proteins. They harbor a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. Both domains have been shown to determine the specific membrane-targeting of SNX1. SNX1 and SNX2 are components of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures effcient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex.


Pssm-ID: 132769 [Multi-domain]  Cd Length: 114  Bit Score: 61.83  E-value: 5.24e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 122 AKFTVYKILVKKTPEE----SWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKR--WFKDNYNADFLEDRQLGLQAFLQNL 195
Cdd:cd06859   16 SAYVVYRVTTKTNLPDfkksEFSVLRRYSDFLWLYERLVEKYPGRIVPPPPEKqaVGRFKVKFEFIEKRRAALERFLRRI 95
                         90
                 ....*....|....*.
gi 966959103 196 VAHKDIANCLAVREFL 211
Cdd:cd06859   96 AAHPVLRKDPDFRLFL 111
PX_RUN cd07277
The phosphoinositide binding Phox Homology domain of uncharacterized proteins containing PX ...
124-198 7.66e-11

The phosphoinositide binding Phox Homology domain of uncharacterized proteins containing PX and RUN domains; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized proteins containing an N-terminal RUN domain and a C-terminal PX domain. PX domain harboring proteins have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction. The RUN domain is found in GTPases in the Rap and Rab families and may play a role in Ras-like signaling pathways.


Pssm-ID: 132810  Cd Length: 118  Bit Score: 58.90  E-value: 7.66e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 966959103 124 FTVYKILVKKTPEEsWVVFRRYTDFSRLNDKLKEMFPGFR-LALPPKRWFkDNYNADFLEDRQLGLQAFLQNLVAH 198
Cdd:cd07277   18 HHVYQVYIRIRDDE-WNVYRRYSEFYELHKKLKKKFPVVRsFDFPPKKAI-GNKDAKFVEERRKRLQVYLRRVVNT 91
PX_IRAS cd06875
The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor ...
111-211 2.46e-10

The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor Antisera-Selected; The PX domain is a phosphoinositide binding (PI) module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Imidazoline Receptor Antisera-Selected (IRAS), also called nischarin, contains an N-terminal PX domain, leucine rich repeats, and a predicted coiled coil domain. The PX domain of IRAS binds to phosphatidylinositol-3-phosphate in membranes. Together with the coiled coil domain, it is essential for the localization of IRAS to endosomes. IRAS has been shown to interact with integrin and inhibit cell migration. Its interaction with alpha5 integrin causes a redistribution of the receptor from the cell surface to endosomal structures, suggesting that IRAS may function as a sorting nexin (SNX) which regulates the endosomal trafficking of integrin. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132785  Cd Length: 116  Bit Score: 57.29  E-value: 2.46e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 111 TILGYEVMEeraKFTVYKILVKKTPEEsWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFkDNYNADFLEDRQLGLQA 190
Cdd:cd06875    7 RIPSAETVE---GYTVYIIEVKVGSVE-WTVKHRYSDFAELHDKLVAEHKVDKDLLPPKKLI-GNKSPSFVEKRRKELEI 81
                         90       100
                 ....*....|....*....|.
gi 966959103 191 FLQNLVAHKDIANCLAVREFL 211
Cdd:cd06875   82 YLQTLLSFFQKTMPRELAHFL 102
PX_SNX8_Mvp1p_like cd06866
The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p; The PX ...
141-211 3.12e-10

The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX8 and the yeast counterpart Mvp1p are involved in sorting and delivery of late-Golgi proteins, such as carboxypeptidase Y, to vacuoles.


Pssm-ID: 132776  Cd Length: 105  Bit Score: 56.47  E-value: 3.12e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 966959103 141 VFRRYTDFSRLNDKLKEMFPgFRL--ALPPKRwFKDNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06866   32 VYRRYSDFVWLHEYLLKRYP-YRMvpALPPKR-IGGSADREFLEARRRGLSRFLNLVARHPVLSEDELVRTFL 102
PX_UP2_fungi cd06869
The phosphoinositide binding Phox Homology domain of uncharacterized fungal proteins; The PX ...
91-211 1.93e-09

The phosphoinositide binding Phox Homology domain of uncharacterized fungal proteins; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized fungal proteins containing a PX domain. PX domain harboring proteins have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction.


Pssm-ID: 132779  Cd Length: 119  Bit Score: 54.98  E-value: 1.93e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  91 RETEEQNPETVIWEDRPSTPtilgyevmeeRAKFtVYKILVKK--TPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPP 168
Cdd:cd06869   11 RVTETAGRLSSKKAYFVNRS----------KHHY-EFIIRVRRegEEYRTIYVARRYSDFKKLHHDLKKEFPGKKLPKLP 79
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 966959103 169 KRwfkdnyNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06869   80 HK------DKLPREKLRLSLRQYLRSLLKDPEVAHSSILQEFL 116
PX_SNX_like cd06865
The phosphoinositide binding Phox Homology domain of SNX-like proteins; The PX domain is a ...
103-211 3.01e-09

The phosphoinositide binding Phox Homology domain of SNX-like proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. This subfamily is composed of uncharacterized proteins, predominantly from plants, with similarity to sorting nexins. A few members show a similar domain architecture as a subfamily of sorting nexins, containing a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX-BAR structural unit is known to determine specific membrane localization.


Pssm-ID: 132775  Cd Length: 120  Bit Score: 54.35  E-value: 3.01e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 103 WEDRPSTPTILGyevmeerAKFTVYKILVKKTPEES----WVVFRRYTDFSRLNDKLKEMFPGFrlALPPkRWFK----- 173
Cdd:cd06865    9 KEQEPSRVPLGG-------PPYISYKVTTRTNIPSYthgeFTVRRRFRDVVALADRLAEAYRGA--FVPP-RPDKsvves 78
                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 966959103 174 -DNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06865   79 qVMQSAEFIEQRRVALEKYLNRLAAHPVIGLSDELRVFL 117
PX_SNX25 cd06878
The phosphoinositide binding Phox Homology domain of Sorting Nexin 25; The PX domain is a ...
133-212 3.35e-09

The phosphoinositide binding Phox Homology domain of Sorting Nexin 25; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. The function of SNX25 is not yet known. It has been found in exosomes from human malignant pleural effusions. SNX25 shows the same domain architecture as SNX13 and SNX14, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.


Pssm-ID: 132788  Cd Length: 127  Bit Score: 54.30  E-value: 3.35e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 133 KTPEESWVVFRRYTDFSRLNDKLKEMFPGFR---LALPPKRWFKDNYNAdFLEDRQLGLQAFLQNLVAHKDIANCLAVRE 209
Cdd:cd06878   44 ESISSGWVVTRKLSEFHDLHRKLKECSSWLKkveLPSLSKKWFKSIDKK-FLDKSKNQLQKYLQFILEDETLCQSEALYS 122

                 ...
gi 966959103 210 FLC 212
Cdd:cd06878  123 FLS 125
PX_SNX20_21_like cd07279
The phosphoinositide binding Phox Homology domain of Sorting Nexins 20 and 21; The PX domain ...
117-214 8.55e-09

The phosphoinositide binding Phox Homology domain of Sorting Nexins 20 and 21; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX20, SNX21, and similar proteins. SNX20 interacts with P-Selectin glycoprotein ligand-1 (PSGL-1), a surface-expressed mucin that acts as a ligand for the selectin family of adhesion proteins. It may function in the sorting and cycling of PSGL-1 into endosomes. SNX21, also called SNX-L, is distinctly and highly-expressed in fetal liver and may be involved in protein sorting and degradation during embryonic liver development.


Pssm-ID: 132812  Cd Length: 112  Bit Score: 52.72  E-value: 8.55e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 117 VMEERAKFTVYKILVKKTPEESW---VVFRRYTDFSRLNDKLKEMFPGFRLALP-PKRWFKDNYNADFLEDRQLGLQAFL 192
Cdd:cd07279   11 VKEGEKKYVVYQLAVVQTGDPDTqpaFIERRYSDFLKLYKALRKQHPQLMAKVSfPRKVLMGNFSSELIAERSRAFEQFL 90
                         90       100
                 ....*....|....*....|..
gi 966959103 193 QNLVAHKDIANCLAVREFLCLD 214
Cdd:cd07279   91 GHILSIPNLRDSKAFLDFLQGP 112
PX_SNX4 cd06864
The phosphoinositide binding Phox Homology domain of Sorting Nexin 4; The PX domain is a ...
124-211 9.35e-09

The phosphoinositide binding Phox Homology domain of Sorting Nexin 4; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX4 is involved in recycling traffic from the sorting endosome (post-Golgi endosome) back to the late Golgi. It shows a similar domain architecture as SNX1-2, among others, containing a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. SNX4 is implicated in the regulation of plasma membrane receptor trafficking and interacts with receptors for EGF, insulin, platelet-derived growth factor and the long form of the leptin receptor.


Pssm-ID: 132774  Cd Length: 129  Bit Score: 53.14  E-value: 9.35e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVK--------KTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLA-LPPKR----WFK---DNYNADFLEDRQLG 187
Cdd:cd06864   23 YTVYLIETKiveheseeGLSKKLSSLWRRYSEFELLRNYLVVTYPYVIVPpLPEKRamfmWQKlssDTFDPDFVERRRAG 102
                         90       100
                 ....*....|....*....|....
gi 966959103 188 LQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06864  103 LENFLLRVAGHPELCQDKIFLEFL 126
PX_SNX7_30_like cd06860
The phosphoinositide binding Phox Homology domain of Sorting Nexins 7 and 30; The PX domain is ...
124-211 3.17e-08

The phosphoinositide binding Phox Homology domain of Sorting Nexins 7 and 30; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. This subfamily consists of SNX7, SNX30, and similar proteins. They harbor a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-6, SNX8, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of the sorting nexins in this subfamily has yet to be elucidated.


Pssm-ID: 132770  Cd Length: 116  Bit Score: 51.18  E-value: 3.17e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVKKT----PEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFK---DNYNADFLEDRQLGLQAFLQNL 195
Cdd:cd06860   18 YITYRVTTKTTrsefDSSEYSVRRRYQDFLWLRQKLEESHPTHIIpPLPEKHSVKgllDRFSPEFVATRMRALHKFLNRI 97
                         90
                 ....*....|....*.
gi 966959103 196 VAHKDIANCLAVREFL 211
Cdd:cd06860   98 VEHPVLSFNEHLKVFL 113
PX_SNX41_42 cd06867
The phosphoinositide binding Phox Homology domain of fungal Sorting Nexins 41 and 42; The PX ...
124-211 2.50e-07

The phosphoinositide binding Phox Homology domain of fungal Sorting Nexins 41 and 42; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX41 and SNX42 (also called Atg20p) form dimers with SNX4, and are required in protein recycling from the sorting endosome (post-Golgi endosome) back to the late Golgi in yeast.


Pssm-ID: 132777  Cd Length: 112  Bit Score: 48.40  E-value: 2.50e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVKKTpeeswVVFRRYTDFSRLNDKLKEMFPGFRLA-LPPKRWFKDNY--------NADFLEDRQLGLQAFLQN 194
Cdd:cd06867   18 YIVYVIRLGGS-----EVKRRYSEFESLRKNLTRLYPTLIIPpIPEKHSLKDYAkkpskaknDAKIIERRKRMLQRFLNR 92
                         90
                 ....*....|....*..
gi 966959103 195 LVAHKDIANCLAVREFL 211
Cdd:cd06867   93 CLQHPILRNDIVFQKFL 109
PX_PI3K_C2 cd06883
The phosphoinositide binding Phox Homology Domain of Class II Phosphoinositide 3-Kinases; The ...
111-211 3.43e-07

The phosphoinositide binding Phox Homology Domain of Class II Phosphoinositide 3-Kinases; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The Phosphoinositide 3-Kinase (PI3K) family of enzymes catalyzes the phosphorylation of the 3-hydroxyl group of the inositol ring of phosphatidylinositol. PI3Ks play an important role in a variety of fundamental cellular processes, including cell motility, the Ras pathway, vesicle trafficking and secretion, immune cell activation and apoptosis. They are also involved in the regulation of clathrin-mediated membrane trafficking as well as ATP-dependent priming of neurosecretory granule exocytosis. PI3Ks are divided into three main classes (I, II, and III) based on their substrate specificity, regulation, and domain structure. Class II PI3Ks preferentially use PI as a substrate to produce PI3P, but can also phosphorylate PI4P to produce PI(3,4)P2. They function as monomers and do not associate with any regulatory subunits. Class II enzymes contain an N-terminal Ras binding domain, a lipid binding C2 domain, a PI3K homology domain of unknown function, an ATP-binding cataytic domain, a PX domain, and a second C2 domain at the C-terminus. Class II PI3Ks include three vertebrate isoforms (alpha, beta, and gamma), the Drosophila PI3K_68D, and similar proteins.


Pssm-ID: 132793  Cd Length: 109  Bit Score: 48.12  E-value: 3.43e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 111 TILGYEVMEERAKFTVY--KILVKKTPEESWVvFRRYTDFSRLNDKLKEMFPGFRLALPPKRW---FKDNYNADflEDRQ 185
Cdd:cd06883    3 SVFGFQKRYSPEKYYIYvvKVTRENQTEPSFV-FRTFEEFQELHNKLSLLFPSLKLPSFPARVvlgRSHIKQVA--ERRK 79
                         90       100
                 ....*....|....*....|....*..
gi 966959103 186 LGLQAFLQNLV-AHKDIANCLAVREFL 211
Cdd:cd06883   80 IELNSYLKSLFnASPEVAESDLVYTFF 106
PX_SNX22 cd06880
The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a ...
113-217 3.54e-07

The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX22 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. The biological function of SNX22 is not yet known.


Pssm-ID: 132790  Cd Length: 110  Bit Score: 48.04  E-value: 3.54e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 113 LGYEVMEERAKFTVYKILVKkTPEESWVVFRRYTDFSRLNDKLKEMF--PGFrlalPPKRwfKDNYNADFLEDRQLGLQA 190
Cdd:cd06880    8 YRLEVDESEKPYTVFTIEVL-VNGRRHTVEKRYSEFHALHKKLKKSIktPDF----PPKR--VRNWNPKVLEQRRQGLEA 80
                         90       100
                 ....*....|....*....|....*..
gi 966959103 191 FLQNLVAHKDIANCLAvrEFLCLDDPP 217
Cdd:cd06880   81 YLQGLLKINELPKQLL--DFLGVRHFP 105
PX_SNX1 cd07281
The phosphoinositide binding Phox Homology domain of Sorting Nexin 1; The PX domain is a ...
124-211 3.80e-07

The phosphoinositide binding Phox Homology domain of Sorting Nexin 1; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX1 is both membrane associated and a cytosolic protein that exists as a tetramer in protein complexes. It can associate reversibly with membranes of the endosomal compartment, thereby coating these vesicles. SNX1 is a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures efficient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex. SNX1 contains a Bin/Amphiphysin/Rvs (BAR) domain C-terminal to the PX domain. The PX domain of SNX1 specifically binds phosphatidylinositol-3-phosphate (PI3P) and PI(3,5)P2, while the BAR domain detects membrane curvature. Both domains help determine the specific membrane-targeting of SNX1, which is localized to a microdomain in early endosomes where it regulates cation-independent mannose-6-phosphate receptor retrieval to the trans Golgi network.


Pssm-ID: 132814  Cd Length: 124  Bit Score: 48.52  E-value: 3.80e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVKKT----PEESWVVFRRYTDFSRLNDKLKEMFP--GFRLALPPKRWF----------KDNYNADFLEDRQLG 187
Cdd:cd07281   18 YVVYKVTTQTSllmfRSKHFTVKRRFSDFLGLYEKLSEKHSqnGFIVPPPPEKSLigmtkvkvgkEDSSSAEFLERRRAA 97
                         90       100
                 ....*....|....*....|....
gi 966959103 188 LQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd07281   98 LERYLQRIVSHPSLLQDPDVREFL 121
COG5391 COG5391
Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function ...
56-255 4.70e-07

Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function prediction only];


Pssm-ID: 227680 [Multi-domain]  Cd Length: 524  Bit Score: 51.34  E-value: 4.70e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  56 SVPDQMDNTSPVCSSPLIRTKFTGAASSIEYSTRPRETEEQNpetviwedrpstptilgyevMEERAKFTVYKILVKKT- 134
Cdd:COG5391  103 SLRDMLSLLLPTSLQPPLSTSHTILDYFISSTVSNPQSLTLL--------------------VDSRDKHTSYEIITVTNl 162
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 135 ------PEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRW----FKDNYNADFLEDRQLGLQAFLQNLVAHKDIAN 203
Cdd:COG5391  163 psfqlrESRPLVVRRRYSDFESLHSILIKLLPLCAIpPLPSKKSnseyYGDRFSDEFIEERRQSLQNFLRRVSTHPLLSN 242
                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 966959103 204 CLAVReflclddppgpfDSLEESRAFCETLEETNYRLQKELLEKQKEMESLK 255
Cdd:COG5391  243 YKNSK------------SWESHSTLLSSFIENRKSVPTPLSLDLTSTTQELD 282
PX_KIF16B_SNX23 cd06874
The phosphoinositide binding Phox Homology domain of KIF16B kinesin or Sorting Nexin 23; The ...
117-197 7.46e-07

The phosphoinositide binding Phox Homology domain of KIF16B kinesin or Sorting Nexin 23; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. KIF16B, also called sorting nexin 23 (SNX23), is a family-3 kinesin which harbors an N-terminal kinesin motor domain containing ATP and microtubule binding sites, a ForkHead Associated (FHA) domain, and a C-terminal PX domain. The PX domain of KIF16B binds to phosphatidylinositol-3-phosphate (PI3P) in early endosomes and plays a role in the transport of early endosomes to the plus end of microtubules. By regulating early endosome plus end motility, KIF16B modulates the balance between recycling and degradation of receptors. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132784  Cd Length: 127  Bit Score: 47.76  E-value: 7.46e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 117 VMEERAKFTVYKILVK-KTPEESWVVFRRYTDFSRLNDKLKEMFPGF-RLALPPKRWFkDNYNADFLEDRQLGLQAFLQN 194
Cdd:cd06874    9 VLRGQGKDEHFEFEVKiTVLDETWTVFRRYSRFRELHKTMKLKYPEVaALEFPPKKLF-GNKSERVAKERRRQLETYLRN 87

                 ...
gi 966959103 195 LVA 197
Cdd:cd06874   88 FFS 90
PX_p47phox cd06887
The phosphoinositide binding Phox Homology domain of the p47phox subunit of NADPH oxidase; The ...
112-210 1.21e-06

The phosphoinositide binding Phox Homology domain of the p47phox subunit of NADPH oxidase; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. p47phox is a cytosolic subunit of the phagocytic NADPH oxidase complex (also called Nox2 or gp91phox), which plays a key role in the ability of phagocytes to defend against bacterial infections. NADPH oxidase catalyzes the transfer of electrons from NADPH to oxygen during phagocytosis forming superoxide and reactive oxygen species. p47phox is required for activation of NADH oxidase and plays a role in translocation. It contains an N-terminal PX domain, two Src Homology 3 (SH3) domains, and a C-terminal domain that contains PxxP motifs for binding SH3 domains. The PX domain of p47phox is unique in that it contains two distinct basic pockets on the membrane-binding surface: one preferentially binds phosphatidylinositol-3,4-bisphosphate [PI(3,4)P2] and is analogous to the PI3P-binding pocket of p40phox, while the other binds anionic phospholipids such as phosphatidic acid or phosphatidylserine. Simultaneous binding in the two pockets results in increased membrane affinity. The PX domain of p47phox is also involved in protein-protein interaction.


Pssm-ID: 132797  Cd Length: 118  Bit Score: 46.75  E-value: 1.21e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 112 ILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFP--------GFRL--ALPPKRWFKDNYNAdfl 181
Cdd:cd06887    6 LLGFEKRFVPSQHYVYMFLVKWQDLSEKLVYRRFTEIYEFHKTLKEMFPieagdinkENRIipHLPAPKWFDGQRAA--- 82
                         90       100       110
                 ....*....|....*....|....*....|
gi 966959103 182 EDRQLGLQAFLQNLV-AHKDIANCLAVREF 210
Cdd:cd06887   83 ENRQGTLTEYCSTLLsLPPKISRCPHVLDF 112
PX_SNX19 cd06893
The phosphoinositide binding Phox Homology domain of Sorting Nexin 19; The PX domain is a ...
141-213 1.69e-06

The phosphoinositide binding Phox Homology domain of Sorting Nexin 19; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX19 contains an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some SNXs. These domains are also found in SNX13 and SNX14, which also contain a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNX19 interacts with IA-2, a major autoantigen found in type-1 diabetes. It inhibits the conversion of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] to PI(3,4,5)P3, which leads in the decrease of protein phosphorylation in the Akt signaling pathway, resulting in apoptosis. SNX19 may also be implicated in coronary heart disease and thyroid oncocytic tumors.


Pssm-ID: 132803 [Multi-domain]  Cd Length: 132  Bit Score: 46.77  E-value: 1.69e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 141 VFRRYTDFSRLNDKLKEMfPGFRLALP---PKRWFKD----NYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCL 213
Cdd:cd06893   53 VNRRFREFLTLQTRLEEN-PKFRKIMNvkgPPKRLFDlpfgNMDKDKIEARRGLLETFLRQLCSIPEISNSEEVQEFLAY 131
PX_SNX27 cd06886
The phosphoinositide binding Phox Homology domain of Sorting Nexin 27; The PX domain is a ...
117-211 5.52e-06

The phosphoinositide binding Phox Homology domain of Sorting Nexin 27; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX27 contains an N-terminal PDZ domain followed by a PX domain and a Ras-Associated (RA) domain. It binds G protein-gated potassium (Kir3) channels, which play a role in neuronal excitability control, through its PDZ domain. SNX27 downregulates Kir3 channels by promoting their movement in the endosome, reducing surface expression and increasing degradation. SNX27 also associates with 5-hydroxytryptamine type 4 receptor (5-HT4R), cytohesin associated scaffolding protein (CASP), and diacylglycerol kinase zeta, and may play a role in their intracellular trafficking and endocytic recycling. The SNX27 PX domain preferentially binds to phosphatidylinositol-3-phosphate (PI3P) and is important for targeting to the early endosome.


Pssm-ID: 132796  Cd Length: 106  Bit Score: 44.71  E-value: 5.52e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 117 VMEERAKFTVYKILVKktpeESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRW-FKdnYNADFLEDRQLGLQAFLQNL 195
Cdd:cd06886   14 VEQNGEKFVVYNIYMA----GRQLCSRRYREFANLHQNLKKEFPDFQFPKLPGKWpFS--LSEQQLDARRRGLEQYLEKV 87
                         90
                 ....*....|....*.
gi 966959103 196 VAHKDIANCLAVREFL 211
Cdd:cd06886   88 CSIRVIGESDIMQDFL 103
PX_SNX9_18_like cd06862
The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18; The PX domain is ...
123-215 1.24e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX9, SNX18, and similar proteins. They contain an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain. SNX9 is localized to plasma membrane endocytic sites and acts primarily in clathrin-mediated endocytosis, while SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.


Pssm-ID: 132772  Cd Length: 125  Bit Score: 44.23  E-value: 1.24e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 123 KFTVYKILVKKTPEEswvVFRRYTDFSRLNDKLKEMFPGfrLALP--PKRWFKDNYNADFLEDRQLGLQAFLQNLVAHKD 200
Cdd:cd06862   19 SFIAYQITPTHTNVT---VSRRYKHFDWLYERLVEKYSC--IAIPplPEKQVTGRFEEDFIEKRRERLELWMNRLARHPV 93
                         90
                 ....*....|....*
gi 966959103 201 IANCLAVREFLCLDD 215
Cdd:cd06862   94 LSQSEVFRHFLTCTD 108
PX_SNX10 cd06898
The phosphoinositide binding Phox Homology domain of Sorting Nexin 10; The PX domain is a ...
122-197 1.53e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexin 10; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX10 may be involved in the regulation of endosome homeostasis. Its expression induces the formation of giant vacuoles in mammalian cells.


Pssm-ID: 132808  Cd Length: 113  Bit Score: 43.47  E-value: 1.53e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 122 AKFTVYKILVKKT----PEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKDNYNADFLEDRQLGLQAFLQNLV 196
Cdd:cd06898   16 GSYTDYEIFLHTNsmcfTLKTSCVRRRYSEFVWLRNRLQKNALLIQLpSLPPKNLFGRFNNEGFIEERQQGLQDFLEKVL 95

                 .
gi 966959103 197 A 197
Cdd:cd06898   96 Q 96
PX_Atg24p cd06863
The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation ...
135-211 1.61e-05

The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation protein; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The yeast Atg24p is a sorting nexin (SNX) which is involved in membrane fusion events at the vacuolar surface during pexophagy. This is facilitated via binding of Atg24p to phosphatidylinositol 3-phosphate (PI3P) through its PX domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132773  Cd Length: 118  Bit Score: 43.43  E-value: 1.61e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 135 PEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFK----DNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVRE 209
Cdd:cd06863   34 SRKEFKVRRRYSDFVFLHECLSNDFPACVVpPLPDKHRLEyitgDRFSPEFITRRAQSLQRFLRRISLHPVLSQSKILHQ 113

                 ..
gi 966959103 210 FL 211
Cdd:cd06863  114 FL 115
PX_SNX18 cd07286
The phosphoinositide binding Phox Homology domain of Sorting Nexin 18; The PX domain is a ...
118-211 1.93e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexin 18; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX18, like SNX9, contains an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature. The PX-BAR structural unit helps determine specific membrane localization. SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.


Pssm-ID: 132819  Cd Length: 127  Bit Score: 43.50  E-value: 1.93e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 118 MEERAKFTVYK-----ILVKKTPEESWV-VFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDRQLGLQAF 191
Cdd:cd07286    5 IDDPTKQTKFKgmksyISYKLVPSHTGLqVHRRYKHFDWLYARLAEKFPVISVPHIPEKQATGRFEEDFISKRRKGLIWW 84
                         90       100
                 ....*....|....*....|
gi 966959103 192 LQNLVAHKDIANCLAVREFL 211
Cdd:cd07286   85 MDHMCSHPVLARCDAFQHFL 104
PX_SNX14 cd06877
The phosphoinositide binding Phox Homology domain of Sorting Nexin 14; The PX domain is a ...
106-198 3.07e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexin 14; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX14 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. It is expressed in the embryonic nervous system of mice, and is co-expressed in the motoneurons and the anterior pituary with Islet-1. SNX14 shows a similar domain architecture as SNX13, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.


Pssm-ID: 132787  Cd Length: 119  Bit Score: 42.75  E-value: 3.07e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 106 RPSTPTILGYEVMEERAKFTVYKILVK------KTPE-ESWVVFRRYTDFSRLNDKLKEMFPGFRLA-LPPKRWFKDNyN 177
Cdd:cd06877    4 RVSIPYVEMRRDPSNGERIYVFCIEVErndrraKGHEpQHWSVLRRYNEFYVLESKLTEFHGEFPDApLPSRRIFGPK-S 82
                         90       100
                 ....*....|....*....|.
gi 966959103 178 ADFLEDRQLGLQAFLQNLVAH 198
Cdd:cd06877   83 YEFLESKREIFEEFLQKLLQK 103
PX_SNX21 cd07301
The phosphoinositide binding Phox Homology domain of Sorting Nexin 21; The PX domain is a ...
117-213 4.15e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexin 21; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX21, also called SNX-L, is distinctly and highly-expressed in fetal liver and may be involved in protein sorting and degradation during embryonic liver development.


Pssm-ID: 132834  Cd Length: 112  Bit Score: 42.10  E-value: 4.15e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 117 VMEERAKFTVYKILV------KKTPEeswVVFRRYTDFSRLNDKLKEMFPG--FRLALPPKRwFKDNYNADFLEDRQLGL 188
Cdd:cd07301   11 VQDAHSKYVLYTIYViqtgqyDPSPA---YISRRYSDFERLHRRLRRLFGGemAGVSFPRKR-LRKNFTAETIAKRSRAF 86
                         90       100
                 ....*....|....*....|....*
gi 966959103 189 QAFLQNLVAHKDIANCLAVREFLCL 213
Cdd:cd07301   87 EQFLCHLHSLPELRASPAFLEFFYL 111
PX_HS1BP3 cd06868
The phosphoinositide binding Phox Homology domain of HS1BP3; The PX domain is a ...
140-214 5.62e-05

The phosphoinositide binding Phox Homology domain of HS1BP3; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Hematopoietic lineage cell-specific protein-1 (HS1) binding protein 3 (HS1BP3) associates with HS1 proteins through their SH3 domains, suggesting a role in mediating signaling. It has been reported that HS1BP3 might affect the IL-2 signaling pathway in hematopoietic lineage cells. Mutations in HS1BP3 may also be associated with familial Parkinson disease and essential tremor. HS1BP3 contains a PX domain, a leucine zipper, motifs similar to immunoreceptor tyrosine-based inhibitory motif and proline-rich regions. The PX domain interacts with PIs and plays a role in targeting proteins to PI-enriched membranes.


Pssm-ID: 132778  Cd Length: 120  Bit Score: 42.01  E-value: 5.62e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 966959103 140 VVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKdnyNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd06868   48 MVSKKYSEFEELYKKLSEKYPGTILpPLPRKALFV---SESDIRERRAAFNDFMRFISKDEKLANCPELLEFLGVK 120
PX_UP1_plant cd06879
The phosphoinositide binding Phox Homology domain of uncharacterized plant proteins; The PX ...
141-214 1.00e-04

The phosphoinositide binding Phox Homology domain of uncharacterized plant proteins; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized fungal proteins containing a PX domain. PX domain harboring proteins have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction.


Pssm-ID: 132789  Cd Length: 138  Bit Score: 41.93  E-value: 1.00e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 966959103 141 VFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKdNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLD 214
Cdd:cd06879   65 VLRRFNDFLKLHTDLKKLFPKKKLpAAPPKGLLR-MKNRALLEERRHSLEEWMGKLLSDIDLSRSVPVASFLELE 138
PX_SNX15_like cd06881
The phosphoinositide binding Phox Homology domain of Sorting Nexin 15-like proteins; The PX ...
119-210 2.87e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexin 15-like proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily have similarity to sorting nexin 15 (SNX15), which contains an N-terminal PX domain and a C-terminal Microtubule Interacting and Trafficking (MIT) domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNX15 plays a role in protein trafficking processes in the endocytic pathway and the trans-Golgi network. The PX domain of SNX15 interacts with the PDGF receptor and is responsible for the membrane association of the protein. Other members of this subfamily contain an additional C-terminal kinase domain, similar to human RPK118, which binds sphingosine kinase and the antioxidant peroxiredoxin-3 (PRDX3). RPK118 may be involved in the transport of proteins such as PRDX3 from the cytoplasm to its site of function in the mitochondria.


Pssm-ID: 132791  Cd Length: 117  Bit Score: 40.00  E-value: 2.87e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 119 EERAKFTVYKILV----KKTPEE--SWVVFRRYTDFSRL---------NDKLKEMFPGFrlalPPKRWFKdNYNADFLED 183
Cdd:cd06881   12 RHKKGYTEYKITSkvfsRSVPEDvsEVVVWKRYSDFKKLhrelsrlhkQLYLSGSFPPF----PKGKYFG-RFDAAVIEE 86
                         90       100
                 ....*....|....*....|....*..
gi 966959103 184 RQLGLQAFLQNLVAHKDIANCLAVREF 210
Cdd:cd06881   87 RRQAILELLDFVGNHPALYQSSAFQQF 113
PX_SNX17_31 cd06885
The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain ...
144-211 3.17e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Members of this subfamily include sorting nexin 17 (SNX17), SNX31, and similar proteins. They contain an N-terminal PX domain followed by a truncated FERM (4.1, ezrin, radixin, and moesin) domain and a unique C-terminal region. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX17 is known to regulate the trafficking and processing of a number of proteins. It binds some members of the low-density lipoprotein receptor (LDLR) family such as LDLR, VLDLR, ApoER2, and others, regulating their endocytosis. It also binds P-selectin and may regulate its lysosomal degradation. SNX17 is highly expressed in neurons. It binds amyloid precursor protein (APP) and may be involved in its intracellular trafficking and processing to amyloid beta peptide, which plays a central role in the pathogenesis of Alzheimer's disease. The biological function of SNX31 is unknown.


Pssm-ID: 132795  Cd Length: 104  Bit Score: 39.62  E-value: 3.17e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 966959103 144 RYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKDNYNAdfLEDRQLGLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd06885   34 RYSQLHGLNEQLKKEFGNRKLpPFPPKKLLPLTPAQ--LEERRLQLEKYLQAVVQDPRIANSDIFNSFL 100
PX_PI3K_C2_alpha cd07289
The phosphoinositide binding Phox Homology Domain of the Alpha Isoform of Class II ...
111-211 3.94e-04

The phosphoinositide binding Phox Homology Domain of the Alpha Isoform of Class II Phosphoinositide 3-Kinases; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The Phosphoinositide 3-Kinase (PI3K) family of enzymes catalyzes the phosphorylation of the 3-hydroxyl group of the inositol ring of phosphatidylinositol. PI3Ks play an important role in a variety of fundamental cellular processes, including cell motility, the Ras pathway, vesicle trafficking and secretion, immune cell activation and apoptosis. PI3Ks are divided into three main classes (I, II, and III) based on their substrate specificity, regulation, and domain structure. Class II PI3Ks preferentially use PI as a substrate to produce PI3P, but can also phosphorylate PI4P to produce PI(3,4)P2. They function as monomers and do not associate with any regulatory subunits. Class II enzymes contain an N-terminal Ras binding domain, a lipid binding C2 domain, a PI3K homology domain of unknown function, an ATP-binding cataytic domain, a PX domain, and a second C2 domain at the C-terminus. The class II alpha isoform, PI3K-C2alpha, plays key roles in clathrin assembly and clathrin-mediated membrane trafficking, insulin signaling, vascular smooth muscle contraction, and the priming of neurosecretory granule exocytosis. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction.


Pssm-ID: 132822  Cd Length: 109  Bit Score: 39.53  E-value: 3.94e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 111 TILGYEVMEERAKFTVYKI-LVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRL-ALPPKRWFKDNYNADFLEDRQLGL 188
Cdd:cd07289    3 SVFTYHKRYNPDKHYIYVVrILREGQIEPSFVFRTFDEFQELHNKLSILFPLWKLpGFPNKMVLGRTHIKDVAAKRKVEL 82
                         90       100
                 ....*....|....*....|....
gi 966959103 189 QAFLQNLV-AHKDIANCLAVREFL 211
Cdd:cd07289   83 NSYIQSLMnSSTEVAECDLVYTFF 106
PX_SNX7 cd07284
The phosphoinositide binding Phox Homology domain of Sorting Nexin 7; The PX domain is a ...
124-198 5.71e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexin 7; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX7 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-6, SNX8, SNX30, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of SNX7 has yet to be elucidated.


Pssm-ID: 132817  Cd Length: 116  Bit Score: 39.19  E-value: 5.71e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVKKTPEE----SWVVFRRYTDFSRLNDKLKEMFPgfRLALPP------KRWFKDNYNADFLEDRQLGLQAFLQ 193
Cdd:cd07284   18 FITYRVMTKTSRSEfdssEFEVRRRYQDFLWLKGRLEEAHP--TLIIPPlpekfvMKGMVERFNEDFIETRRKALHKFLN 95

                 ....*
gi 966959103 194 NLVAH 198
Cdd:cd07284   96 RIADH 100
PX_Vps5p cd06861
The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps5p; The PX domain ...
125-211 6.11e-04

The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps5p; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Vsp5p is the yeast counterpart of human SNX1 and is part of the retromer complex, which functions in the endosome-to-Golgi retrieval of vacuolar protein sorting receptor Vps10p, the Golgi-resident membrane protein A-ALP, and endopeptidase Kex2. The PX domain of Vps5p binds phosphatidylinositol-3-phosphate (PI3P). Similar to SNX1, Vps5p contains a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. Both domains have been shown to determine the specific membrane-targeting of SNX1.


Pssm-ID: 132771  Cd Length: 112  Bit Score: 38.87  E-value: 6.11e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 125 TVYKILVKKT---PEESWV-VFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDRQLGLQAFLQNLVAHKD 200
Cdd:cd06861   19 TVYTVRTRTTspnFEVSSFsVLRRYRDFRWLYRQLQNNHPGVIVPPPPEKQSVGRFDDNFVEQRRAALEKMLRKIANHPV 98
                         90
                 ....*....|.
gi 966959103 201 IANCLAVREFL 211
Cdd:cd06861   99 LQKDPDFRLFL 109
PX_SNX2 cd07282
The phosphoinositide binding Phox Homology domain of Sorting Nexin 2; The PX domain is a ...
124-211 7.06e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexin 2; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX2 is a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures efficient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex. Similar to SNX1, SNX2 contains a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX domain of SNX2 preferentially binds phosphatidylinositol-3-phosphate (PI3P), but not PI(3,4,5)P3. Studies on mice deficient with SNX1 and/or SNX2 suggest that they provide an essential function in embryogenesis and are functionally redundant.


Pssm-ID: 132815  Cd Length: 124  Bit Score: 38.88  E-value: 7.06e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 124 FTVYKILVKKT----PEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPP------------KRWFKDNYNADFLEDRQLG 187
Cdd:cd07282   18 YMAYRVTTKTSlsmfSRSEFSVRRRFSDFLGLHSKLASKYLHVGYIVPPapeksivgmtkvKVGKEDSSSTEFVEKRRAA 97
                         90       100
                 ....*....|....*....|....
gi 966959103 188 LQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd07282   98 LERYLQRTVKHPTLLQDPDLRQFL 121
PX_p40phox cd06882
The phosphoinositide binding Phox Homology domain of the p40phox subunit of NADPH oxidase; The ...
105-211 1.04e-03

The phosphoinositide binding Phox Homology domain of the p40phox subunit of NADPH oxidase; The PX domain is a phosphoinositide binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. p40phox contains an N-terminal PX domain, a central SH3 domain that binds p47phox, and a C-terminal PB1 domain that interacts with p67phox. It is a cytosolic subunit of the phagocytic NADPH oxidase complex (also called Nox2 or gp91phox) which plays a crucial role in the cellular response to bacterial infection. NADPH oxidase catalyzes the transfer of electrons from NADPH to oxygen during phagocytosis forming superoxide and reactive oxygen species. p40phox positively regulates NADPH oxidase in both phosphatidylinositol-3-phosphate (PI3P)-dependent and PI3P-independent manner. The PX domain is a phospholipid-binding module involved in the membrane targeting of proteins. The p40phox PX domain binds to PI3P, an abundant lipid in phagosomal membranes, playing an important role in the localization of NADPH oxidase. The PX domain of p40phox is also involved in protein-protein interaction.


Pssm-ID: 132792  Cd Length: 123  Bit Score: 38.57  E-value: 1.04e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 105 DRPSTPTILGyevMEERAKFT---VYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPG---------FRLALPPKRWF 172
Cdd:cd06882    1 DVAVSATIAD---IEEKRGFTnyyVFVIEVKTKGGSKYLIYRRYRQFFALQSKLEERFGPeagssaydcTLPTLPGKIYV 77
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 966959103 173 KDnyNADFLEDRQLGLQAFLQNLVA-HKDIANCLAVREFL 211
Cdd:cd06882   78 GR--KAEIAERRIPLLNRYMKELLSlPVWVLMDEDVRLFF 115
PX_PI3K_C2_68D cd06884
The phosphoinositide binding Phox Homology Domain of Class II Phosphoinositide 3-Kinases ...
111-210 1.28e-03

The phosphoinositide binding Phox Homology Domain of Class II Phosphoinositide 3-Kinases similar to the Drosophila PI3K_68D protein; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The Phosphoinositide 3-Kinase (PI3K) family of enzymes catalyzes the phosphorylation of the 3-hydroxyl group of the inositol ring of phosphatidylinositol. PI3Ks play an important role in a variety of fundamental cellular processes, including cell motility, the Ras pathway, vesicle trafficking and secretion, immune cell activation and apoptosis. PI3Ks are divided into three main classes (I, II, and III) based on their substrate specificity, regulation, and domain structure. Class II PI3Ks preferentially use PI as a substrate to produce PI3P, but can also phosphorylate PI4P to produce PI(3,4)P2. They function as monomers and do not associate with any regulatory subunits. Class II enzymes contain an N-terminal Ras binding domain, a lipid binding C2 domain, a PI3K homology domain of unknown function, an ATP-binding cataytic domain, a PX domain, and a second C2 domain at the C-terminus. PI3K_68D is a novel PI3K which is widely expressed throughout the Drosophila life cycle. In vitro, it has been shown to phosphorylate PI and PI4P. It is involved in signaling pathways that affect pattern formation of Drosophila wings.


Pssm-ID: 132794  Cd Length: 111  Bit Score: 38.17  E-value: 1.28e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 111 TILGYEVMEERAKFTVYKILV-KKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFL-EDRQLGL 188
Cdd:cd06884    5 TVVGFQKRYDPEKYYVYVVEVtRENQASPQHVFRTYKEFLELYQKLCRKFPLAKLHPLSTGSHVGRSNIKSVaEKRKQDI 84
                         90       100
                 ....*....|....*....|...
gi 966959103 189 QAFLQNLVA-HKDIANCLAVREF 210
Cdd:cd06884   85 QQFLNSLFKmAEEVSHSDLVYTF 107
PX_SNX20 cd07300
The phosphoinositide binding Phox Homology domain of Sorting Nexin 20; The PX domain is a ...
122-211 2.48e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 20; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX20 interacts with P-Selectin glycoprotein ligand-1 (PSGL-1), a surface-expressed mucin that acts as a ligand for the selectin family of adhesion proteins. The PX domain of SNX20 binds PIs and targets the SNX20/PSGL-1 complex to endosomes. SNX20 may function in the sorting and cycling of PSGL-1 into endosomes.


Pssm-ID: 132833  Cd Length: 114  Bit Score: 37.10  E-value: 2.48e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 122 AKFTVYKILVKKTPE---ESWVVFRRYTDFSRLNdklKEMFPGFRLALP----PKRWFKDNYNADFLEDRQLGLQAFLQN 194
Cdd:cd07300   16 SKHVVYQIIVIQTGSfdcNKVVIERRYSDFLKLH---QELLSDFSEELEdvvfPKKKLTGNFSEEIIAERRVALRDYLTL 92
                         90
                 ....*....|....*..
gi 966959103 195 LVAHKDIANCLAVREFL 211
Cdd:cd07300   93 LYSLRFVRRSQAFQDFL 109
Mplasa_alph_rch TIGR04523
helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of ...
231-336 2.73e-03

helix-rich Mycoplasma protein; Members of this family occur strictly within a subset of Mycoplasma species. Members average 750 amino acids in length, including signal peptide. Sequences are predicted (Jpred 3) to be almost entirely alpha-helical. These sequences show strong periodicity (consistent with long alpha helical structures) and low complexity rich in D,E,N,Q, and K. Genes encoding these proteins are often found in tandem. The function is unknown.


Pssm-ID: 275316 [Multi-domain]  Cd Length: 745  Bit Score: 39.62  E-value: 2.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103  231 ETLEETNYRLQKELLEKQKEMESLKKLLSEKQLHIDTLENRIRTLSVEPEESLDVSETEGEQILKVKSSALEVDQDVLD- 309
Cdd:TIGR04523 352 TNSESENSEKQRELEEKQNEIEKLKKENQSYKQEIKNLESQINDLESKIQNQEKLNQQKDEQIKKLQQEKELLEKEIERl 431
                          90       100
                  ....*....|....*....|....*..
gi 966959103  310 EESRADNKPCLSFSEPENAVSEIEVAE 336
Cdd:TIGR04523 432 KETIIKNNSEIKDLTNQDSVKELIIKN 458
PX_SNX3 cd07293
The phosphoinositide binding Phox Homology domain of Sorting Nexin 3; The PX domain is a ...
121-211 2.87e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 3; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX3 associates with early endosomes through a PX domain-mediated interaction with phosphatidylinositol-3-phosphate (PI3P). It associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer. SNX3 is required for the formation of multivesicular bodies, which function as transport intermediates to late endosomes. It also promotes cell surface expression of the amiloride-sensitive epithelial Na+ channel (ENaC), which is critical in sodium homeostasis and maintenance of extracellular fluid volume.


Pssm-ID: 132826  Cd Length: 123  Bit Score: 37.28  E-value: 2.87e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 121 RAKFTVYKILVK------KTPEESwvVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKD--------NYNADFLEDRQL 186
Cdd:cd07293   16 RGRFTTYEIRLKtnlpifKLKEST--VRRRYSDFEWLRSELERESKVVVPPLPGKALFRQlpfrgddgIFDDSFIEERKQ 93
                         90       100
                 ....*....|....*....|....*
gi 966959103 187 GLQAFLQNLVAHKDIANCLAVREFL 211
Cdd:cd07293   94 GLEQFLNKVAGHPLAQNERCLHMFL 118
PX_SNX3_like cd06894
The phosphoinositide binding Phox Homology domain of Sorting Nexin 3 and related proteins; The ...
121-212 4.04e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 3 and related proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily is composed of SNX3, SNX12, and fungal Grd19. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. SNX3/Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.


Pssm-ID: 132804  Cd Length: 123  Bit Score: 36.67  E-value: 4.04e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 121 RAKFTVYKILVK------KTPEESwvVFRRYTDFSRLNDKL----KEMFPgfrlALPPKRW-----FKDN---YNADFLE 182
Cdd:cd06894   16 KKRFTDYEVRMRtnlpvfKKKESS--VRRRYSDFEWLRSELerdsKIVVP----PLPGKALkrqlpFRGDdgiFEEEFIE 89
                         90       100       110
                 ....*....|....*....|....*....|
gi 966959103 183 DRQLGLQAFLQNLVAHKDIANCLAVREFLC 212
Cdd:cd06894   90 ERRKGLETFINKVAGHPLAQNEKCLHMFLQ 119
PX_Bem1p cd06890
The phosphoinositide binding Phox Homology domain of Bem1p; The PX domain is a ...
127-213 4.06e-03

The phosphoinositide binding Phox Homology domain of Bem1p; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily bear similarity to Saccharomyces cerevisiae Bem1p, containing two Src Homology 3 (SH3) domains at the N-terminus, a central PX domain, and a C-terminal PB1 domain. Bem1p is a scaffolding protein that is critical for proper Cdc42p activation during bud formation in yeast. During budding and mating, Bem1p migrates to the plasma membrane where it can serve as an adaptor for Cdc42p and some other proteins. Bem1p also functions as an effector of the G1 cyclin Cln3p and the cyclin-dependent kinase Cdc28p in promoting vacuolar fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of Bem1p specifically binds phosphatidylinositol-4-phosphate (PI4P).


Pssm-ID: 132800  Cd Length: 112  Bit Score: 36.50  E-value: 4.06e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 127 YKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFP------GFRLALP--PKRwFKDNYNADFLEDRQLGLQAFLQNLVAH 198
Cdd:cd06890   17 YRVRATLSDGKTRYLCRYYQDFYKLHIALLDLFPaeagrnSSKRILPylPGP-VTDVVNDSISLKRLNDLNEYLNELINL 95
                         90
                 ....*....|....*.
gi 966959103 199 K-DIANCLAVREFLCL 213
Cdd:cd06890   96 PaYIQTSEVVRDFFAN 111
PX_Grd19 cd07295
The phosphoinositide binding Phox Homology domain of fungal Grd19; The PX domain is a ...
141-198 4.11e-03

The phosphoinositide binding Phox Homology domain of fungal Grd19; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.


Pssm-ID: 132828  Cd Length: 116  Bit Score: 36.71  E-value: 4.11e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 966959103 141 VFRRYTDFSRLNDKLKEMFPgfRLALP--PKRWFKDNYNADFLEDRQLGLQAFLQNLVAH 198
Cdd:cd07295   40 VRRRYSDFEYFRDILERESP--RVMIPplPGKIFTNRFSDEVIEERRQGLETFLQSVAGH 97
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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