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Conserved domains on  [gi|1622946365|ref|XP_014996077|]
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LOW QUALITY PROTEIN: glycine cleavage system H protein, mitochondrial [Macaca mulatta]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 51-170 1.97e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 1.97e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:COG0509     7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKS 170
Cdd:COG0509    87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 51-170 1.97e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 1.97e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:COG0509     7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKS 170
Cdd:COG0509    87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
51-170 6.13e-65

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 194.99  E-value: 6.13e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAEN 130
Cdd:PRK01202    8 LKYTKSHEWVRVEGDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEALEDS 87

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1622946365 131 PGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKS 170
Cdd:PRK01202   88 PELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 51-171 5.66e-63

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 189.85  E-value: 5.66e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTEN-GIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:pfam01597   1 LYYTKEHEWVKVEGdGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSI 171
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 53-147 7.47e-49

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 153.07  E-value: 7.47e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  53 FTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENP 131
Cdd:cd06848     1 YTKDHEWVKVEgDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 1622946365 132 GLVNKSCYEDGWLIKM 147
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 52-166 7.63e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 144.21  E-value: 7.63e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  52 KFTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAEN 130
Cdd:TIGR00527   7 RYSSEHEWVRVEgDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALEDS 86

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1622946365 131 PGLVNKSCYEDGWLIKMTLSNP-SELDELMSEEAYEK 166
Cdd:TIGR00527  87 PELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYEA 123
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 51-170 1.97e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 1.97e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:COG0509     7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKS 170
Cdd:COG0509    87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
51-170 6.13e-65

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 194.99  E-value: 6.13e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAEN 130
Cdd:PRK01202    8 LKYTKSHEWVRVEGDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEALEDS 87

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1622946365 131 PGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKS 170
Cdd:PRK01202   88 PELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 51-171 5.66e-63

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 189.85  E-value: 5.66e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTEN-GIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:pfam01597   1 LYYTKEHEWVKVEGdGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSI 171
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 53-147 7.47e-49

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 153.07  E-value: 7.47e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  53 FTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENP 131
Cdd:cd06848     1 YTKDHEWVKVEgDGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                          90
                  ....*....|....*.
gi 1622946365 132 GLVNKSCYEDGWLIKM 147
Cdd:cd06848    81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 52-166 7.63e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 144.21  E-value: 7.63e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  52 KFTEKHEW*TTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAEN 130
Cdd:TIGR00527   7 RYSSEHEWVRVEgDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALEDS 86

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1622946365 131 PGLVNKSCYEDGWLIKMTLSNP-SELDELMSEEAYEK 166
Cdd:TIGR00527  87 PELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYEA 123
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 51-173 1.64e-39

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 130.89  E-value: 1.64e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  51 RKFTEKHEW*TTEN-GIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAE 129
Cdd:PRK13380   14 RYTIKNHEWLRLEGdGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVVEVNEALED 93

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1622946365 130 NPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE 173
Cdd:PRK13380   94 SPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENID 137
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 69-150 8.94e-16

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 69.09  E-value: 8.94e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622946365  69 VGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMT 148
Cdd:PRK00624   21 LGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDIQPINNAPESEGWFVVVQ 100


                  ..
gi 1622946365 149 LS 150
Cdd:PRK00624  101 LD 102
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 68-146 2.49e-12

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 58.99  E-value: 2.49e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1622946365  68 TVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPglvnkscYEDGWLIK 146
Cdd:cd06663     1 TILIPDLAQHLGDGTVVKWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKV-------EGDTPLVK 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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