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Conserved domains on  [gi|967375435|ref|XP_014976869|]
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zinc finger protein ZIC 2 isoform X2 [Macaca mulatta]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
zf_ZIC pfam18366
Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family ...
 250-295 3.52e-23

Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family proteins found in Eukaryotes. In humans, there are five members of the Zic family that are involved in human congenital anomalies. One of them, ZIC3, causes X-linked heterotaxy (HTX1), which is a left-right axis disturbance that manifests as variable combinations of heart malformation, altered lung lobation, splenic abnormality and gastrointestinal malrotation. Zic faily proteins contain multiple zinc finger domains (ZFD), which are generally composed of five tandemly repeated C2H2 zinc finger (ZF) motifs. Sequence comparison analysis reveal that this N-terminal ZF (ZF1) domain of the Zic zinc finger domains is unique in that it possesses more amino acid residues (6-38 amino acids) between the two cysteine residues of the C2H2 motif compared to Gli and Glis ZF1s or any of the other ZFs (ZF2-5) in the Gli/Glis/Zic superfamily of proteins. Mutations in cysteine 253 (C253S) or histidine 286 (H286R) in ZIC3 ZF1, which are found in heterotaxy patients, result in extranuclear localization of the mutant ZIC3 protein. Furthermore, mutations in the evolutionarily conserved amino acid residues (C253, W255, C268, H281 and H286) of ZF1 generally impair nuclear localization.


:

Pssm-ID: 436441  Cd Length: 45  Bit Score: 91.29  E-value: 3.52e-23
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 967375435  250 QCIKQELICKWIDPEQlSNPKKSCNKTFSTMHELVTHVSVEHVGGP 295
Cdd:pfam18366   1 QPIKQELSCKWIDPEQ-PAPKKPCNKTFSTMHEIVTHLTVEHVGGP 45
SFP1 super family cl25788
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 329-413 2.26e-08

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


The actual alignment was detected with superfamily member COG5189:

Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 55.88  E-value: 2.26e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 329 GEKPFPCPFPGCGKVFARSENLKIHKRthtgekpfqcefEG-CDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSS 407
Cdd:COG5189  346 DGKPYKCPVEGCNKKYKNQNGLKYHML------------HGhQNQKLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNG 413


                 ....*.
gi 967375435 408 LRKHMK 413
Cdd:COG5189  414 LKYHRK 419
 
Name Accession Description Interval E-value
zf_ZIC pfam18366
Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family ...
 250-295 3.52e-23

Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family proteins found in Eukaryotes. In humans, there are five members of the Zic family that are involved in human congenital anomalies. One of them, ZIC3, causes X-linked heterotaxy (HTX1), which is a left-right axis disturbance that manifests as variable combinations of heart malformation, altered lung lobation, splenic abnormality and gastrointestinal malrotation. Zic faily proteins contain multiple zinc finger domains (ZFD), which are generally composed of five tandemly repeated C2H2 zinc finger (ZF) motifs. Sequence comparison analysis reveal that this N-terminal ZF (ZF1) domain of the Zic zinc finger domains is unique in that it possesses more amino acid residues (6-38 amino acids) between the two cysteine residues of the C2H2 motif compared to Gli and Glis ZF1s or any of the other ZFs (ZF2-5) in the Gli/Glis/Zic superfamily of proteins. Mutations in cysteine 253 (C253S) or histidine 286 (H286R) in ZIC3 ZF1, which are found in heterotaxy patients, result in extranuclear localization of the mutant ZIC3 protein. Furthermore, mutations in the evolutionarily conserved amino acid residues (C253, W255, C268, H281 and H286) of ZF1 generally impair nuclear localization.


Pssm-ID: 436441  Cd Length: 45  Bit Score: 91.29  E-value: 3.52e-23
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 967375435  250 QCIKQELICKWIDPEQlSNPKKSCNKTFSTMHELVTHVSVEHVGGP 295
Cdd:pfam18366   1 QPIKQELSCKWIDPEQ-PAPKKPCNKTFSTMHEIVTHLTVEHVGGP 45
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 329-413 2.26e-08

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 55.88  E-value: 2.26e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 329 GEKPFPCPFPGCGKVFARSENLKIHKRthtgekpfqcefEG-CDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSS 407
Cdd:COG5189  346 DGKPYKCPVEGCNKKYKNQNGLKYHML------------HGhQNQKLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNG 413


                 ....*.
gi 967375435 408 LRKHMK 413
Cdd:COG5189  414 LKYHRK 419
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
243-414 1.27e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 1.27e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 243 FFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTMHELVTHVSVEHVGGPEQSNHVCfWEECPREGKPFKAKYKLVN 322
Cdd:COG5048  235 LSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ-CNISFSRSSPLTRHLRSVN 313

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 323 HIRVhtGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKhmHVHTSDKPYLCKMcDKSY 402
Cdd:COG5048  314 HSGE--SLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFSPLLNNEP--PQSLQQYKDLKND-KKSE 388

                        170
                 ....*....|..
gi 967375435 403 THPSSLRKHMKS 414
Cdd:COG5048  389 TLSNSCIRNFKR 400
zf-H2C2_2 pfam13465
Zinc-finger double domain;
349-376 1.45e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.51  E-value: 1.45e-04
                          10        20
                  ....*....|....*....|....*...
gi 967375435  349 NLKIHKRTHTGEKPFQCEFegCDRRFAN 376
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPE--CGKSFKS 26
 
Name Accession Description Interval E-value
zf_ZIC pfam18366
Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family ...
 250-295 3.52e-23

Zic proteins zinc finger domain; This is the ZF1 (Zinc Finger 1) domain found in Zic family proteins found in Eukaryotes. In humans, there are five members of the Zic family that are involved in human congenital anomalies. One of them, ZIC3, causes X-linked heterotaxy (HTX1), which is a left-right axis disturbance that manifests as variable combinations of heart malformation, altered lung lobation, splenic abnormality and gastrointestinal malrotation. Zic faily proteins contain multiple zinc finger domains (ZFD), which are generally composed of five tandemly repeated C2H2 zinc finger (ZF) motifs. Sequence comparison analysis reveal that this N-terminal ZF (ZF1) domain of the Zic zinc finger domains is unique in that it possesses more amino acid residues (6-38 amino acids) between the two cysteine residues of the C2H2 motif compared to Gli and Glis ZF1s or any of the other ZFs (ZF2-5) in the Gli/Glis/Zic superfamily of proteins. Mutations in cysteine 253 (C253S) or histidine 286 (H286R) in ZIC3 ZF1, which are found in heterotaxy patients, result in extranuclear localization of the mutant ZIC3 protein. Furthermore, mutations in the evolutionarily conserved amino acid residues (C253, W255, C268, H281 and H286) of ZF1 generally impair nuclear localization.


Pssm-ID: 436441  Cd Length: 45  Bit Score: 91.29  E-value: 3.52e-23
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 967375435  250 QCIKQELICKWIDPEQlSNPKKSCNKTFSTMHELVTHVSVEHVGGP 295
Cdd:pfam18366   1 QPIKQELSCKWIDPEQ-PAPKKPCNKTFSTMHEIVTHLTVEHVGGP 45
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 329-413 2.26e-08

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 55.88  E-value: 2.26e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 329 GEKPFPCPFPGCGKVFARSENLKIHKRthtgekpfqcefEG-CDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSS 407
Cdd:COG5189  346 DGKPYKCPVEGCNKKYKNQNGLKYHML------------HGhQNQKLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNG 413


                 ....*.
gi 967375435 408 LRKHMK 413
Cdd:COG5189  414 LKYHRK 419
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
330-407 1.05e-06

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.85  E-value: 1.05e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 967375435 330 EKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSS 407
Cdd:COG5048   29 NAPRPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHNNPSDLNSKSLPLSNSKAS 106
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
320-415 1.11e-05

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 47.38  E-value: 1.11e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 320 LVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDrKKHMHVHTSDKPYLCKMCD 399
Cdd:COG5048   49 LTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHNNPSDLNSKSLPLSNSKASSS-SLSSSSSNSNDNNLLSSHS 127

                         90
                 ....*....|....*.
gi 967375435 400 KSYTHPSSLRKHMKSP 415
Cdd:COG5048  128 LPPSSRDPQLPDLLSI 143
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
243-414 1.27e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 44.30  E-value: 1.27e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 243 FFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTMHELVTHVSVEHVGGPEQSNHVCfWEECPREGKPFKAKYKLVN 322
Cdd:COG5048  235 LSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ-CNISFSRSSPLTRHLRSVN 313

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 967375435 323 HIRVhtGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEFEGCDRRFANSSDRKKhmHVHTSDKPYLCKMcDKSY 402
Cdd:COG5048  314 HSGE--SLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFSPLLNNEP--PQSLQQYKDLKND-KKSE 388

                        170
                 ....*....|..
gi 967375435 403 THPSSLRKHMKS 414
Cdd:COG5048  389 TLSNSCIRNFKR 400
zf-H2C2_2 pfam13465
Zinc-finger double domain;
349-376 1.45e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 38.51  E-value: 1.45e-04
                          10        20
                  ....*....|....*....|....*...
gi 967375435  349 NLKIHKRTHTGEKPFQCEFegCDRRFAN 376
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPE--CGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
320-346 7.60e-04

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 36.58  E-value: 7.60e-04
                          10        20
                  ....*....|....*....|....*..
gi 967375435  320 LVNHIRVHTGEKPFPCPFpgCGKVFAR 346
Cdd:pfam13465   2 LKRHMRTHTGEKPYKCPE--CGKSFKS 26
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
312-383 2.15e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 40.06  E-value: 2.15e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 967375435 312 KPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLKIHKRTHTGEKPFQCEfegCDRRFANSSDRKKH 383
Cdd:COG5048  396 RNFKRDSNLSLHIITHLSFRPYNCKNPPCSKSFNRHYNLIPHKKIHTNHAPLLCS---ILKSFRRDLDLSNH 464
zf-H2C2_2 pfam13465
Zinc-finger double domain;
381-404 5.26e-03

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 34.27  E-value: 5.26e-03
                          10        20
                  ....*....|....*....|....
gi 967375435  381 KKHMHVHTSDKPYLCKMCDKSYTH 404
Cdd:pfam13465   3 KRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 333-357 6.76e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 33.81  E-value: 6.76e-03
                          10        20
                  ....*....|....*....|....*
gi 967375435  333 FPCPfpGCGKVFARSENLKIHKRTH 357
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 393-414 8.64e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 33.81  E-value: 8.64e-03
                          10        20
                  ....*....|....*....|..
gi 967375435  393 YLCKMCDKSYTHPSSLRKHMKS 414
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRT 22
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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