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Conserved domains on  [gi|966994380|ref|XP_014973980|]
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mannose-P-dolichol utilization defect 1 protein isoform X2 [Macaca mulatta]

Protein Classification

cystinosin/MPDU1 family protein( domain architecture ID 707892)

cystinosin/MPDU1 family protein similar to homo sapiens mannose-P-dolichol utilization defect 1, a cystinosin homolog that required for normal utilization of mannose-dolichol phosphate (Dol-P-Man) in the synthesis of N-linked and O-linked oligosaccharides and GPI anchors

Gene Ontology:  GO:0006810|GO:0016020
PubMed:  22363504
TCDB:  2.A.43

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
2A43 super family cl28663
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 46-161 1.00e-23

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


The actual alignment was detected with superfamily member TIGR00951:

Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 95.31  E-value: 1.00e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380   46 WLAHFLlPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALFLMLQTITICFLVM 114
Cdd:TIGR00951  15 WSISFY-PQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVFFTLHAILICFIVL 93

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380  115 HYRGQTVKG-------------VAFLACYGLVLLVLLSPLTPLTVVTLLQASNVPAVVVG 161
Cdd:TIGR00951  94 HQCGDYERGwqrvsnpwilrilVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVK 153
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 46-161 1.00e-23

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 95.31  E-value: 1.00e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380   46 WLAHFLlPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALFLMLQTITICFLVM 114
Cdd:TIGR00951  15 WSISFY-PQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVFFTLHAILICFIVL 93

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380  115 HYRGQTVKG-------------VAFLACYGLVLLVLLSPLTPLTVVTLLQASNVPAVVVG 161
Cdd:TIGR00951  94 HQCGDYERGwqrvsnpwilrilVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVK 153
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 51-94 2.68e-06

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 43.65  E-value: 2.68e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 966994380   51 LLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSITNNFPFS 94
Cdd:pfam04193  17 YLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPS 60
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 51-80 4.36e-04

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 36.66  E-value: 4.36e-04
                           10        20        30
                   ....*....|....*....|....*....|
gi 966994380    51 LLPQVFKILGAKSAEGLSLQSVMLELVALT 80
Cdd:smart00679   3 LLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
 
Name Accession Description Interval E-value
2A43 TIGR00951
Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and ...
 46-161 1.00e-23

Lysosomal Cystine Transporter; [Transport and binding proteins, Amino acids, peptides and amines]


Pssm-ID: 130026 [Multi-domain]  Cd Length: 220  Bit Score: 95.31  E-value: 1.00e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380   46 WLAHFLlPQVFKILGAKSAEGLSLQSVMLELVALTGTMVY--------SITNNFPFSSWG---EALFLMLQTITICFLVM 114
Cdd:TIGR00951  15 WSISFY-PQIIKNWRRKSAEGLSFDFVMLNLVGFTAYVIFnflqlycwSITNEFPLSSPGvtqNDVFFTLHAILICFIVL 93

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 966994380  115 HYRGQTVKG-------------VAFLACYGLVLLVLLSPLTPLTVVTLLQASNVPAVVVG 161
Cdd:TIGR00951  94 HQCGDYERGwqrvsnpwilrilVALLACFATLLVALLSPITPLAFVTMLSYIKVAVTLVK 153
PQ-loop pfam04193
PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of ...
 51-94 2.68e-06

PQ loop repeat; Members of this family are all membrane bound proteins possessing a pair of repeats each spanning two transmembrane helices connected by a loop. The PQ motif found on loop 2 is critical for the localization of cystinosin to lysosomes. However, the PQ motif appears not to be a general lysosome-targeting motif. It is thought likely to possess a more general function. Most probably this involves a glutamine residue. Family members are membrane transporters since two members, cystinosin and PQLC2, transport cystine and cationic amino acids, respectively, across the lysosomal membrane. The 2nd PQ-loop of cystinosin hosts the substrate-coupled H+ binding site underlying its H+ symport mechanism, suggesting that PQ-loop repeats have functional significance. It is thus likely that PQ-loop-containing proteins act as a family of membrane transporters. Some transport cystine and cationic amino acids, respectively, across the lysosomal membrane. Others transport lysine and or arginine across the lysosomal membrane in order to maintain the acidic homoeostasis.


Pssm-ID: 461220 [Multi-domain]  Cd Length: 61  Bit Score: 43.65  E-value: 2.68e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 966994380   51 LLPQVFKILGAKSAEGLSLQSVMLELVALTGTMVYSITNNFPFS 94
Cdd:pfam04193  17 YLPQIIKNYKRKSTEGLSILFLLLWLLGDIFYLLYLILNGFPPS 60
CTNS smart00679
Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, ...
 51-80 4.36e-04

Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins; Function unknown, but likely to be associated with the glycosylation machinery.


Pssm-ID: 128923 [Multi-domain]  Cd Length: 32  Bit Score: 36.66  E-value: 4.36e-04
                           10        20        30
                   ....*....|....*....|....*....|
gi 966994380    51 LLPQVFKILGAKSAEGLSLQSVMLELVALT 80
Cdd:smart00679   3 LLPQIIKNYRRKSTEGLSILFVLLWLLGDI 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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