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Conserved domains on  [gi|755536129|ref|XP_011241951|]
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proteasome activator complex subunit 4 isoform X1 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
BLM10_mid super family cl24919
Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or ...
330-828 7.23e-138

Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_mid is the middle ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. Thus Blm10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.


The actual alignment was detected with superfamily member pfam16507:

Pssm-ID: 465150  Cd Length: 503  Bit Score: 435.80  E-value: 7.23e-138
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   330 LAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWltpvPESHKLTDEDVTDFVQCIIQPVLLAMF 409
Cdd:pfam16507    1 LEKLIQSIETFFHPSNSGSWTKPLSKFVHYLIKQFHKRWNEEEKPDCKT----PEERRLTPAIKKEFVLILRPVLLLGIF 76
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   410 SKTGSL--EAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPegptHMLPLLMR 487
Cdd:pfam16507   77 SKDSDVanYYQSALQYLAYLEPDLILPGILERIYPSLEGLNETHRTIASLKALTRLARFLVSTKLYRV----HLTPLLSL 152
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   488 ALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSS-----VLQERNDLTEIEKE-----LCSATAEFEDFVLQFMDRCFGL 557
Cdd:pfam16507  153 LLPGIDLNDLSKTSNTLNFIVSIASFIPFGDLTKdndegDDEEDDEDFELDDElednaLRSSTTGFEDWLKSFLDRVFTL 232
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   558 IESStleqtrEETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVagRMVADMCRAAVKC 637
Cdd:pfam16507  233 LENL------PDEGKKTGGKLEEGVINKLPQTLDILFESLSDELFDLALDKFFDFVSTNVKHNAV--DAVGIICAALVKR 304
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   638 CPEESLKLFVPHC-YGVITQL--------TMNDDVlneeELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTL-H 707
Cdd:pfam16507  305 DPEKTLKKLFPLLiANIREEIeegassrrTTSEIL----PRDRALVWNLSILNGCVRNSGSALLKYKDELLELLKYLYeK 380
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   708 LTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPseyFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSF 787
Cdd:pfam16507  381 CKGPPGYVLTSILVHSLLSTLTSTYPTEYRLFNPDEWKSP---FDEEHWGKLQFDENLKIDWHVPSEEEIDFAVELFESI 457
                          490       500       510       520
                   ....*....|....*....|....*....|....*....|....*.
gi 755536129   788 LQPELIKLQ-----CCGDGELEMSRDDILQSLTIVHSCLIGSGNLL 828
Cdd:pfam16507  458 VSPALDKLEalmkeMSRPGSDKEWSDDFCRYLLYLRHALSGISTLF 503
 
Name Accession Description Interval E-value
BLM10_mid pfam16507
Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or ...
330-828 7.23e-138

Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_mid is the middle ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. Thus Blm10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.


Pssm-ID: 465150  Cd Length: 503  Bit Score: 435.80  E-value: 7.23e-138
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   330 LAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWltpvPESHKLTDEDVTDFVQCIIQPVLLAMF 409
Cdd:pfam16507    1 LEKLIQSIETFFHPSNSGSWTKPLSKFVHYLIKQFHKRWNEEEKPDCKT----PEERRLTPAIKKEFVLILRPVLLLGIF 76
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   410 SKTGSL--EAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPegptHMLPLLMR 487
Cdd:pfam16507   77 SKDSDVanYYQSALQYLAYLEPDLILPGILERIYPSLEGLNETHRTIASLKALTRLARFLVSTKLYRV----HLTPLLSL 152
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   488 ALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSS-----VLQERNDLTEIEKE-----LCSATAEFEDFVLQFMDRCFGL 557
Cdd:pfam16507  153 LLPGIDLNDLSKTSNTLNFIVSIASFIPFGDLTKdndegDDEEDDEDFELDDElednaLRSSTTGFEDWLKSFLDRVFTL 232
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   558 IESStleqtrEETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVagRMVADMCRAAVKC 637
Cdd:pfam16507  233 LENL------PDEGKKTGGKLEEGVINKLPQTLDILFESLSDELFDLALDKFFDFVSTNVKHNAV--DAVGIICAALVKR 304
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   638 CPEESLKLFVPHC-YGVITQL--------TMNDDVlneeELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTL-H 707
Cdd:pfam16507  305 DPEKTLKKLFPLLiANIREEIeegassrrTTSEIL----PRDRALVWNLSILNGCVRNSGSALLKYKDELLELLKYLYeK 380
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   708 LTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPseyFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSF 787
Cdd:pfam16507  381 CKGPPGYVLTSILVHSLLSTLTSTYPTEYRLFNPDEWKSP---FDEEHWGKLQFDENLKIDWHVPSEEEIDFAVELFESI 457
                          490       500       510       520
                   ....*....|....*....|....*....|....*....|....*.
gi 755536129   788 LQPELIKLQ-----CCGDGELEMSRDDILQSLTIVHSCLIGSGNLL 828
Cdd:pfam16507  458 VSPALDKLEalmkeMSRPGSDKEWSDDFCRYLLYLRHALSGISTLF 503
 
Name Accession Description Interval E-value
BLM10_mid pfam16507
Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or ...
330-828 7.23e-138

Proteasome-substrate-size regulator, mid region; The ordered regions of the yeast BLM10 or PA200 (human homolog), full-length protein encode 32 HEAT repeat (HR)-like modules, each comprising two helices joined by a turn, with adjacent repeats connected by a linker. Whereas a standard HEAT repeat is composed of ~50 residues, the BLM10 HEAT repeats are highly variable. The length of helices ranges from 8 to 35 residues, turns range from 2 to 87 residues, and linkers range from 1 to 88 residues, with the longest linker, between HR21 and HR22, containing additional secondary structures (two strands and three helices). BLM10_mid is the middle ordered region of the three in BLM10. BLM10 is found to surround the proteasome entry pore in the 1.2 MDa complex of proteasome and BLM10 to form a largely closed dome that is expected to restrict access of potential substrates. Thus Blm10 and PA200 are predominantly nuclear and stimulate the degradation of model peptides, although they do not appear to stimulate the degradation of proteins, recognize ubiquitin, or utilize ATP.


Pssm-ID: 465150  Cd Length: 503  Bit Score: 435.80  E-value: 7.23e-138
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   330 LAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERFKKPSWltpvPESHKLTDEDVTDFVQCIIQPVLLAMF 409
Cdd:pfam16507    1 LEKLIQSIETFFHPSNSGSWTKPLSKFVHYLIKQFHKRWNEEEKPDCKT----PEERRLTPAIKKEFVLILRPVLLLGIF 76
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   410 SKTGSL--EAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLNCVIGVARSLVSGSKWFPegptHMLPLLMR 487
Cdd:pfam16507   77 SKDSDVanYYQSALQYLAYLEPDLILPGILERIYPSLEGLNETHRTIASLKALTRLARFLVSTKLYRV----HLTPLLSL 152
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   488 ALPGVDPNDFSKCMITFQFIGTFSTLVPLVDCSS-----VLQERNDLTEIEKE-----LCSATAEFEDFVLQFMDRCFGL 557
Cdd:pfam16507  153 LLPGIDLNDLSKTSNTLNFIVSIASFIPFGDLTKdndegDDEEDDEDFELDDElednaLRSSTTGFEDWLKSFLDRVFTL 232
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   558 IESStleqtrEETETEKMTHLESLVELGLSSTFSTILTQCSKDIFMVALQKVFNFSVSHIFETRVagRMVADMCRAAVKC 637
Cdd:pfam16507  233 LENL------PDEGKKTGGKLEEGVINKLPQTLDILFESLSDELFDLALDKFFDFVSTNVKHNAV--DAVGIICAALVKR 304
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   638 CPEESLKLFVPHC-YGVITQL--------TMNDDVlneeELDKELLWNLQLLSEITRVDGKKLLLYREQLVKILQRTL-H 707
Cdd:pfam16507  305 DPEKTLKKLFPLLiANIREEIeegassrrTTSEIL----PRDRALVWNLSILNGCVRNSGSALLKYKDELLELLKYLYeK 380
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755536129   708 LTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFNKPPseyFPVKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSF 787
Cdd:pfam16507  381 CKGPPGYVLTSILVHSLLSTLTSTYPTEYRLFNPDEWKSP---FDEEHWGKLQFDENLKIDWHVPSEEEIDFAVELFESI 457
                          490       500       510       520
                   ....*....|....*....|....*....|....*....|....*.
gi 755536129   788 LQPELIKLQ-----CCGDGELEMSRDDILQSLTIVHSCLIGSGNLL 828
Cdd:pfam16507  458 VSPALDKLEalmkeMSRPGSDKEWSDDFCRYLLYLRHALSGISTLF 503
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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