cwf21 domain; The cwf21 family is involved in mRNA splicing. It has been isolated as a subcomplex of the splicosome in Schizosaccharomyces pombe. The function of the cwf21 domain is to bind directly to the spliceosomal protein Prp8. Mutations in the cwf21 domain prevent Prp8 from binding. The structure of this domain has recently been solved which shows this domain to be composed of two alpha helices.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 755514420   55 LDHERKRRVELKCMELQEMMEEQGYSEEEIRQKVGTFRQMLMEK 98
Cdd:pfam08312   1 LEHERKREIEVKVLELRDELEEQGLSEEEIEEKVDELRKKLLAE 44

cwf21 domain found in serine/arginine repetitive matrix protein 2; Serine/arginine repetitive matrix protein 2 (SRRM2) is also called 300 kDa nuclear matrix antigen, serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa, SR-related nuclear matrix protein of 300 kDa, Ser/Arg-related nuclear matrix protein of 300 kDa, splicing coactivator subunit SRm300, or Tax-responsive enhancer element-binding protein 803 (TaxREB803). It is required for pre-mRNA splicing as component of the spliceosome. It contains a cwf21 domain at the N-terminus. The cwf21 domain is involved in mRNA splicing; it binds directly to the spliceosomal protein Prp8.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 755514420  36 EEELRAAEPGLVKRAHREILDHERKRRVELKCMELQEMMEEQGYSEEEIRQKVGTFRQMLMEKE 99
Cdd:cd21375    1 EEELRRLEAALVKKPNPDILDHERKRRVELKCLELEEMMEEQGYSEEEIQEKVATFRLMLLEKD 64

cwf21 domain found in fungal complexed with CEF1 protein 21 (CWC21) and similar proteins; This subfamily includes complexed with CEF1 protein 21 (CWC21) from budding yeast, complexed with cdc5 protein 21 (CWF21) from fission yeast, as well as their orthologs, serine/arginine repetitive matrix proteins (SRRM2 and SRRM3) from vertebrates. Both CWC21 and CWF21 are pre-mRNA-splicing factors that may function at or prior to the first catalytic step of splicing at the catalytic center of the spliceosome, together with ISY1. SRRM2 is required for pre-mRNA splicing as a component of the spliceosome. SRRM3 may play a role in regulating breast cancer cell invasiveness. It may be involved in RYBP-mediated breast cancer progression. Members of this family contain a cwf21 domain at the N-terminus. The cwf21 domain is involved in mRNA splicing; it binds directly to the spliceosomal protein Prp8.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 755514420  53 EILDHERKRRVELKCMELQEMMEEQGYSEEEIRQKVGTFRQMLMEK 98
Cdd:cd21372    3 EILEHERKRQIELKCLELRDELEDEGLSEEEIEEKVDELREKLLKE 48

cwf21 domain; The cwf21 domain is involved in mRNA splicing; it binds directly to the spliceosomal protein Prp8. Mutations in the cwf21 domain prevents its binding to Prp8. The domain is composed of two alpha helices. Proteins containing the cwf21 domain include complexed with CEF1 protein 21 (CWC21) from budding yeast, complexed with cdc5 protein 21 (CWF21) from fission yeast, as well as their orthologs, serine/arginine repetitive matrix proteins (SRRM2 and SRRM3) from vertebrates. This domain family also includes U2-associated protein SR140 from Eumetazoa, protein RRC1, and similar proteins from plants.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 755514420  53 EILDHERKRRVELKCMELQEMMEEQG-YSEEEIRQKVGTFRQMLMEK 98
Cdd:cd21369    2 DEEKRAKKREIELKVMELRDELEEQGrKPEQQIQEKVEHYRDKLLQR 48