RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 181 SLNPIAQQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLL 260
Cdd:cd04376    1 SLNPIARQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVLDENHSVHDVAALLKEFFRDMPDPLL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 261 PDDLYMSFLLTATLKPKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDSIGVDGQLVPGNRMTSTNLALVFGTA 340
Cdd:cd04376   81 PRELYTAFIGTALLEPDEQLEALQLLIYLLPPCNCDTLHRLLKFLHTVAEHAADSIDEDGQEVSGNKMTSLNLATIFGPN 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 569009416 341 LLKKGKLANKESRKTKLGIDHYVASVNVVRAMIDNWDILFQVPPHI 386
Cdd:cd04376  161 LLHKQKSGEREFVQASLRIEESTAIINVVQTMIDNYEELFMVSPEL 206

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 181 SLNPIAQQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLL 260
Cdd:cd04376    1 SLNPIARQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVLDENHSVHDVAALLKEFFRDMPDPLL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 261 PDDLYMSFLLTATLKPKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDSIGVDGQLVPGNRMTSTNLALVFGTA 340
Cdd:cd04376   81 PRELYTAFIGTALLEPDEQLEALQLLIYLLPPCNCDTLHRLLKFLHTVAEHAADSIDEDGQEVSGNKMTSLNLATIFGPN 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 569009416 341 LLKKGKLANKESRKTKLGIDHYVASVNVVRAMIDNWDILFQVPPHI 386
Cdd:cd04376  161 LLHKQKSGEREFVQASLRIEESTAIINVVQTMIDNYEELFMVSPEL 206

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416  190 PQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLD-DSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRGPDVDLDlEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 569009416  269 LLTATLKPKD-QVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALL 342
Cdd:pfam00620  81 IEAAKLPDEEeRLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDV-----------NKMNAHNLAIVFGPTLL 144

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 181 SLNPIAQQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLL 260
Cdd:cd04376    1 SLNPIARQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVLDENHSVHDVAALLKEFFRDMPDPLL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 261 PDDLYMSFLLTATLKPKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDSIGVDGQLVPGNRMTSTNLALVFGTA 340
Cdd:cd04376   81 PRELYTAFIGTALLEPDEQLEALQLLIYLLPPCNCDTLHRLLKFLHTVAEHAADSIDEDGQEVSGNKMTSLNLATIFGPN 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 569009416 341 LLKKGKLANKESRKTKLGIDHYVASVNVVRAMIDNWDILFQVPPHI 386
Cdd:cd04376  161 LLHKQKSGEREFVQASLRIEESTAIINVVQTMIDNYEELFMVSPEL 206

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416  190 PQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLD-DSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRGPDVDLDlEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 569009416  269 LLTATLKPKD-QVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALL 342
Cdd:pfam00620  81 IEAAKLPDEEeRLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDV-----------NKMNAHNLAIVFGPTLL 144

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDK---GLDIVLDDSvNVHDVAELLKEFFREMKDPLLPDDLY 265
Cdd:cd04385   15 IPVIVDKCIDFITQHGLMSEGIYRKNGKNSSVKKLLEAFRKdarSVQLREGEY-TVHDVADVLKRFLRDLPDPLLTSELH 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 266 MSFLLTATLKPKDQ-VSALQLLVYLMPPCHSDTLERLLKALHKItENCEDSigvdgqlvpgNRMTSTNLALVFGTALLKK 344
Cdd:cd04385   94 AEWIEAAELENKDErIARYKELIRRLPPINRATLKVLIGHLYRV-QKHSDE----------NQMSVHNLALVFGPTLFQT 162

                        170       180       190
                 ....*....|....*....|....*....|..
gi 569009416 345 GKLANKESRKtklgidhyvaSVNVVRAMIDNW 376
Cdd:cd04385  163 DEHSVGQTSH----------EVKVIEDLIDNY 184

RhoGAP_ARHGAP21: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP21-like proteins. ArhGAP21 is a multi-domain protein, containing RhoGAP, PH and PDZ domains, and is believed to play a role in the organization of the cell-cell junction complex. It has been shown to function as a GAP of Cdc42 and RhoA, and to interact with alpha-catenin and Arf6. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGL-DIVLDD----SVNVhdVAELLKEFFREMKDPLLPDD 263
Cdd:cd04395   18 VPLIVEVCCNIVEARGLETVGIYRVPGNNAAISALQEELNRGGfDIDLQDprwrDVNV--VSSLLKSFFRKLPEPLFTNE 95

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 264 LYMSFLLTATLK-PKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALL 342
Cdd:cd04395   96 LYPDFIEANRIEdPVERLKELRRLIHSLPDHHYETLKHLIRHLKTVADNSEV-----------NKMEPRNLAIVFGPTLV 164

                 .
gi 569009416 343 K 343
Cdd:cd04395  165 R 165

RhoGAP_DLC1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of DLC1-like proteins. DLC1 shows in vitro GAP activity towards RhoA and CDC42. Beside its C-terminal GAP domain, DLC1 also contains a SAM (sterile alpha motif) and a START (StAR-related lipid transfer action) domain. DLC1 has tumor suppressor activity in cell culture. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 180 MSLNPIAQQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPL 259
Cdd:cd04375   11 VNLQRTGQPLPRSIQQAMRWLRNNALDQVGLFRKSGVKSRIQKLRSMIESSTDNVNYDGQQAYDVADMLKQYFRDLPEPL 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 260 LPDDLYMSFLLTATLKPKDQ-VSALQLLVYLMPPCHSDTLERLLKALHKITENcedsigvdgqlVPGNRMTSTNLALVFG 338
Cdd:cd04375   91 LTNKLSETFIAIFQYVPKEQrLEAVQCAILLLPDENREVLQTLLYFLSDVAAN-----------SQENQMTATNLAVCLA 159

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 569009416 339 TAL-----LKKGKLANKESRKTK--LGIDHY------VASVNVVRAMIDNWDILFQVP 383
Cdd:cd04375  160 PSLfhlntSRRENSSPARRMQRKksLGKPDQkelsenKAAHQCLAYMIEECNTLFMVP 217

RhoGAP_myosin_IX: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in class IX myosins. Class IX myosins contain a characteristic head domain, a neck domain, a tail domain which contains a C6H2-zinc binding motif and a RhoGAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:cd04377   15 VPLVLEKLLEHIEMHGLYTEGIYRKSGSANKIKELRQGLDTDPDSVNLEDYPIHVITSVLKQWLRELPEPLMTFELYENF 94

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 569009416 269 LLTATLK-PKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENcEDSigvdgqlvpgNRMTSTNLALVFGTALLK 343
Cdd:cd04377   95 LRAMELEeKQERVRALYSVLEQLPRANLNTLERLIFHLVRVALQ-EEV----------NRMSANALAIVFAPCILR 159

RhoGAP-p50rhoGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p50RhoGAP-like proteins; p50RhoGAP, also known as RhoGAP-1, contains a C-terminal RhoGAP domain and an N-terminal Sec14 domain which binds phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). It is ubiquitously expressed and preferentially active on Cdc42. This subgroup also contains closely related ARHGAP8. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 183 NPIAQQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLLPD 262
Cdd:cd04404   17 NPEQEPIPPVVRETVEYLQAHALTTEGIFRRSANTQVVKEVQQKYNMGEPVDFDQYEDVHLPAVILKTFLRELPEPLLTF 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 263 DLYMSFLLTATLKPKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALL 342
Cdd:cd04404   97 DLYDDIVGFLNVDKEERVERVKQLLQTLPEENYQVLKYLIKFLVQVSAHSDQ-----------NKMTNSNLAVVFGPNLL 165

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 168 PIGQRLLGSKRKMSLnpiaqqipqIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKG-LDIVLDDSVN-VHDVA 245
Cdd:cd04386    8 PLEEHLKRTGREIAL---------PIEACVMCLLETGMNEEGLFRVGGGASKLKRLKAALDAGtFSLPLDEFYSdPHAVA 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 246 ELLKEFFREMKDPLLPDDLYMSFLLTATLKPKDQ-VSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvp 324
Cdd:cd04386   79 SALKSYLRELPDPLLTYNLYEDWVQAANKPDEDErLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDE---------- 148

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 569009416 325 gNRMTSTNLALVFGTALLkkgkLANKESRKTKLGIDHYVASVNVVRAMIDNWDILF 380
Cdd:cd04386  149 -NKMSPSNIAIVLAPNLL----WAKNEGSLAEMAAGTSVHVVAIVELIISHADWFF 199

RhoGAP_GMIP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein) and PARG1 (PTPL1-associated RhoGAP1). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:cd04378   16 VPFIIKKCTSEIENRALGVQGIYRVSGSKARVEKLCQAFENGKDLVELSELSPHDISSVLKLFLRQLPEPLILFRLYNDF 95

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 269 ------LLTATLKPKDQ---------VSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNL 333
Cdd:cd04378   96 ialakeIQRDTEEDKAPntpievnriIRKLKDLLRQLPASNYNTLQHLIAHLYRVAEQFEE-----------NKMSPNNL 164

                        170
                 ....*....|
gi 569009416 334 ALVFGTALLK 343
Cdd:cd04378  165 GIVFGPTLIR 174

RhoGAP_srGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an N-terminal FCH domain, a central RhoGAP domain and a C-terminal SH3 domain; this SH3 domain interacts with the intracellular proline-rich-tail of the Roundabout receptor (Robo). This interaction with Robo then activates the rhoGAP domain which in turn inhibits Cdc42 activity. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 187 QQIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSvNVHD---VAELLKEFFREMKDPLLPDD 263
Cdd:cd04383   16 QAIPLVVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNAFERGEDPLADDQ-NDHDinsVAGVLKLYFRGLENPLFPKE 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 264 LYMSFLLTATLK-PKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALL 342
Cdd:cd04383   95 RFEDLMSCVKLEnPTERVHQIREILSTLPRSVIIVMRYLFAFLNHLSQFSDE-----------NMMDPYNLAICFGPTLM 163

RhoGAP_CdGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to focal adhesions via the interaction with the scaffold protein actopaxin (alpha-parvin). Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 187 QQIPQIVETCCKFIEKHGLSSvGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHD---VAELLKEFFREMKDPLLPDD 263
Cdd:cd04384   16 QDVPQVLKSCTEFIEKHGIVD-GIYRLSGIASNIQRLRHEFDSEQIPDLTKDVYIQDihsVSSLCKLYFRELPNPLLTYQ 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 264 LYMSFLLTATLKPKD-QVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDSigvdgqlvpgnRMTSTNLALVFGTALL 342
Cdd:cd04384   95 LYEKFSEAVSAASDEeRLEKIHDVIQQLPPPHYRTLEFLMRHLSRLAKYCSIT-----------NMHAKNLAIVWAPNLL 163

                 ....*..
gi 569009416 343 KKGKLAN 349
Cdd:cd04384  164 RSKQIES 170

RhoGAP_myosin_IXB: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:cd04407   15 VPIVLEKLLEHVEMHGLYTEGIYRKSGSANRMKELHQLLQADPENVKLENYPIHAITGLLKQWLRELPEPLMTFAQYNDF 94

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 569009416 269 LLTATLKPK-DQVSALQLLVYLMPPCHSDTLERLLKALHKITENcEDSigvdgqlvpgNRMTSTNLALVFGTALLK 343
Cdd:cd04407   95 LRAVELPEKqEQLQAIYRVLEQLPTANHNTLERLIFHLVKVALE-EDV----------NRMSPNALAIVFAPCLLR 159

RhoGAP_chimaerin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of chimaerins. Chimaerins are a family of phorbolester- and diacylglycerol-responsive GAPs specific for the Rho-like GTPase Rac. Chimaerins exist in two alternative splice forms that each contain a C-terminal GAP domain, and a central C1 domain which binds phorbol esters, inducing a conformational change that activates the protein; one splice form is lacking the N-terminal Src homology-2 (SH2) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 188 QIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKG---LDIVLDDSVNVHDVAELLKEFFREMKDPLLPDDL 264
Cdd:cd04372   15 QRPMVVDMCIREIEARGLQSEGLYRVSGFAEEIEDVKMAFDRDgekADISATVYPDINVITGALKLYFRDLPIPVITYDT 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 265 YMSFLLTATL-KPKDQVSALQLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNRMTSTNLALVFGTALLK 343
Cdd:cd04372   95 YPKFIDAAKIsNPDERLEAVHEALMLLPPAHYETLRYLMEHLKRVTLHEKD-----------NKMNAENLGIVFGPTLMR 163

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 569009416 344 KGKLANKESrktklgIDHYVASVNVVRAMIDNWDILF 380
Cdd:cd04372  164 PPEDSALTT------LNDMRYQILIVQLLITNEDVLF 194

RhoGAP_ARHGAP19: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP19-like proteins. The function of ArhGAP19 is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 188 QIPQIVEtcckFIEKHgLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDS-VNVHDVAELLKEFFREMKDPLLPDDLYM 266
Cdd:cd04392   12 QIYQLIE----YLEKN-LRVEGLFRKPGNSARQQELRDLLNSGTDLDLESGgFHAHDCATVLKGFLGELPEPLLTHAHYP 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 267 SFLLTATLKPKD-------------QVSALQLLVYLMPPCHSDTLERLLKALHKiTENCEDSigvdgqlvpgNRMTSTNL 333
Cdd:cd04392   87 AHLQIADLCQFDekgnktsapdkerLLEALQLLLLLLPEENRNLLKLILDLLYQ-TAKHEDK----------NKMSADNL 155

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 569009416 334 ALVFGTALLKKGKLANKESRKTKLGIDhyvasvNVVRAMIDNWDILFQVPPHIQKEV 390
Cdd:cd04392  156 ALLFTPHLICPRNLTPEDLHENAQKLN------SIVTFMIKHSQKLFKAPAYLREDA 206

RhoGAP_fSAC7_BAG7: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal SAC7 and BAG7-like proteins. Both proteins are GTPase activating proteins of Rho1, but differ functionally in vivo: SAC7, but not BAG7, is involved in the control of Rho1-mediated activation of the PKC-MPK1 pathway. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDI---VLDDSVNVHDVAELLKEFFREMKDPLLPDDLY 265
Cdd:cd04396   32 IPVVVAKCGVYLKENATEVEGIFRVAGSSKRIRELQLIFSTPPDYgksFDWDGYTVHDAASVLRRYLNNLPEPLVPLDLY 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 266 MSFLLTATLKPK---------------DQVSAL---QLLVYLMPPCHSDTLERLLKALHKITENCEDsigvdgqlvpgNR 327
Cdd:cd04396  112 EEFRNPLRKRPRilqymkgrineplntDIDQAIkeyRDLITRLPNLNRQLLLYLLDLLAVFARNSDK-----------NL 180

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 569009416 328 MTSTNLALVFGTALLKKGKLAnkesrktkLGIDHYVASVNVVRAMIDNWDIL 379
Cdd:cd04396  181 MTASNLAAIFQPGILSHPDHE--------MDPKEYKLSRLVVEFLIEHQDKF 224

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 188 QIPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDK--GLDIVLDDsVNVHDVAELLKEFFREMKDPLLPDDLY 265
Cdd:cd04373   14 PIPIFLEKCVEFIEATGLETEGIYRVSGNKTHLDSLQKQFDQdhNLDLVSKD-FTVNAVAGALKSFFSELPDPLIPYSMH 92

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 569009416 266 MSFLLTATLKPKDQ-VSALQLLVYLMPPCHSDTLERLLKALHKITENCedsigvdgqlvPGNRMTSTNLALVFGTALLK 343
Cdd:cd04373   93 LELVEAAKINDREQrLHALKELLKKFPPENFDVFKYVITHLNKVSQNS-----------KVNLMTSENLSICFWPTLMR 160

RhoGAP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of PARG1 (PTPL1-associated RhoGAP1). PARG1 was originally cloned as an interaction partner of PTPL1, an intracellular protein-tyrosine phosphatase. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 189 IPQIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELFDKGLDIVLDDSVNVHDVAELLKEFFREMKDPLLPDDLYMSF 268
Cdd:cd04409   16 IPFIIKKCTSEIESRALCLKGIYRVNGAKSRVEKLCQAFENGKDLVELSELSPHDISNVLKLYLRQLPEPLILFRLYNEF 95

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 269 L-----------------LTATLKPKDQVSALQLLVYL------MPPCHSDTLERLLKALHKITENCEDsigvdgqlvpg 325
Cdd:cd04409   96 IglakesqhvnetqeakkNSDKKWPNMCTELNRILLKSkdllrqLPAPNYNTLQFLIVHLHRVSEQAEE----------- 164

                        170
                 ....*....|....*...
gi 569009416 326 NRMTSTNLALVFGTALLK 343
Cdd:cd04409  165 NKMSASNLGIIFGPTLIR 182

RhoGAP_Graf: GTPase-activator protein (GAP) domain for Rho-like GTPases found in GRAF (GTPase regulator associated with focal adhesion kinase); Graf is a multi-domain protein, containing SH3 and PH domains, that binds focal adhesion kinase and influences cytoskeletal changes mediated by Rho proteins. Graf exhibits GAP activity toward RhoA and Cdc42, but only weakly activates Rac1. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569009416 191 QIVETCCKFIEKHGLSSVGIFTIEYSLRRVLELRELF-------DKGLDIVlDDSVNVHDVAELLKEFFREMKDPLLPDD 263
Cdd:cd04374   30 KFVRKCIEAVETRGINEQGLYRVVGVNSKVQKLLSLGldpktstPGDVDLD-NSEWEIKTITSALKTYLRNLPEPLMTYE 108