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Conserved domains on  [gi|568956672|ref|XP_006531000|]
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methenyltetrahydrofolate synthase domain-containing protein isoform X2 [Mus musculus]

Protein Classification

5-formyltetrahydrofolate cyclo-ligase family protein( domain architecture ID 951)

5-formyltetrahydrofolate cyclo-ligase family protein similar to Mus musculus methenyltetrahydrofolate synthase domain-containing protein (MTHFSD), a novel RNA-binding protein abnormally regulated in amyotrophic lateral sclerosis

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
5-FTHF_cyc-lig super family cl00360
5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or ...
 81-278 5.59e-23

5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or methenyl-THF synthetase EC:6.3.3.2 catalyzes the interchange of 5-formyltetrahydrofolate (5-FTHF) to 5-10-methenyltetrahydrofolate, this requires ATP and Mg2+. 5-FTHF is used in chemotherapy where it is clinically known as Leucovorin.


The actual alignment was detected with superfamily member pfam01812:

Pssm-ID: 444864 [Multi-domain]  Cd Length: 186  Bit Score: 95.07  E-value: 5.59e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672   81 KQSIRERI---WDYMESHDIADFPRPVHHRIPNFKGSylagQSIRDLEVFagtqeVKVDPDKPLEGVRFLALQSKKTLLV 157
Cdd:pfam01812   1 KQELRKQLlarRRALSEEERAAQSEALHQRLISLPEY----QKAKRVAAY-----VSVGGEIDTRELIDLLLEEGKRVLL 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672  158 PTPRLRTGLFN--KITPPPGATKDILRKCATSQGVRNFSVPVGLDSSVLVDLVVVgsvAVSEKGWRIGKGEGYADLEYAM 235
Cdd:pfam01812  72 PVPRPGSGHLDmvRFTPYYPEDSLPRGAWGLKEPVEEELRELALGQLDLVLVPGV---AFDRQGYRLGRGGGYYDRYLAR 148

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 568956672  236 MVSMGAvhKGTPVVTIVHDCQVVDIPealVEDHDLTVDYILTP 278
Cdd:pfam01812 149 LQGHGA--KPYTVGLAFDEQLVERLP---VEPHDVPVDEVVTE 186
 
Name Accession Description Interval E-value
5-FTHF_cyc-lig pfam01812
5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or ...
 81-278 5.59e-23

5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or methenyl-THF synthetase EC:6.3.3.2 catalyzes the interchange of 5-formyltetrahydrofolate (5-FTHF) to 5-10-methenyltetrahydrofolate, this requires ATP and Mg2+. 5-FTHF is used in chemotherapy where it is clinically known as Leucovorin.


Pssm-ID: 396398 [Multi-domain]  Cd Length: 186  Bit Score: 95.07  E-value: 5.59e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672   81 KQSIRERI---WDYMESHDIADFPRPVHHRIPNFKGSylagQSIRDLEVFagtqeVKVDPDKPLEGVRFLALQSKKTLLV 157
Cdd:pfam01812   1 KQELRKQLlarRRALSEEERAAQSEALHQRLISLPEY----QKAKRVAAY-----VSVGGEIDTRELIDLLLEEGKRVLL 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672  158 PTPRLRTGLFN--KITPPPGATKDILRKCATSQGVRNFSVPVGLDSSVLVDLVVVgsvAVSEKGWRIGKGEGYADLEYAM 235
Cdd:pfam01812  72 PVPRPGSGHLDmvRFTPYYPEDSLPRGAWGLKEPVEEELRELALGQLDLVLVPGV---AFDRQGYRLGRGGGYYDRYLAR 148

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 568956672  236 MVSMGAvhKGTPVVTIVHDCQVVDIPealVEDHDLTVDYILTP 278
Cdd:pfam01812 149 LQGHGA--KPYTVGLAFDEQLVERLP---VEPHDVPVDEVVTE 186
FAU1 COG0212
5-formyltetrahydrofolate cyclo-ligase [Coenzyme transport and metabolism];
214-281 6.47e-10

5-formyltetrahydrofolate cyclo-ligase [Coenzyme transport and metabolism];


Pssm-ID: 439982 [Multi-domain]  Cd Length: 186  Bit Score: 57.86  E-value: 6.47e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 568956672 214 AVSEKGWRIGKGEGYAD--LEyammvsmgAVHKGTPVVTIVHDCQVVD-IPealVEDHDLTVDYILTPTRV 281
Cdd:COG0212  127 AFDRRGYRLGYGGGYYDrtLA--------RLRPRPLTIGLAFDCQLVDeLP---VEPHDVPLDAIVTEKGV 186
MTHFS_bact TIGR02727
5,10-methenyltetrahydrofolate synthetase; This enzyme, 5,10-methenyltetrahydrofolate ...
 214-278 1.26e-05

5,10-methenyltetrahydrofolate synthetase; This enzyme, 5,10-methenyltetrahydrofolate synthetase, is also called 5-formyltetrahydrofolate cycloligase. Function of bacterial proteins in this family was inferred originally from the known activity of eukaryotic homologs. Recently, activity was shown explicitly for the member from Mycoplasma pneumonia. Members of this family from alpha- and gamma-proteobacteria, designated ygfA, are often found in an operon with 6S structural RNA, and show a similar pattern of high expression during stationary phase. The function may be to deplete folate to slow 1-carbon biosynthetic metabolism. [Central intermediary metabolism, One-carbon metabolism]


Pssm-ID: 274270 [Multi-domain]  Cd Length: 179  Bit Score: 45.34  E-value: 1.26e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 568956672  214 AVSEKGWRIGKGEGYADLEYAMMVsmgavhkgTPVVTIVHDCQVVD-IPealVEDHDLTVDYILTP 278
Cdd:TIGR02727 125 AFDRRGYRLGYGGGYYDRFLARLK--------GITIGLAFDFQLVDeLP---REPHDVPVDAIITE 179
PLN02812 PLN02812
5-formyltetrahydrofolate cyclo-ligase
 214-282 1.40e-03

5-formyltetrahydrofolate cyclo-ligase


Pssm-ID: 178408  Cd Length: 211  Bit Score: 39.63  E-value: 1.40e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 568956672 214 AVSEKGWRIGKGEGYADLEYAMMVSMgAVHKG--TP-VVTIVHDCQVVDIPEALVEDHDLTVDYILTPTRVI 282
Cdd:PLN02812 141 AFDRSGRRLGRGGGYYDTFLSKYQEL-AKEKGwkQPlLVALSYSPQILDEGSVPVDETDVLVDALVTPSGVI 211
 
Name Accession Description Interval E-value
5-FTHF_cyc-lig pfam01812
5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or ...
 81-278 5.59e-23

5-formyltetrahydrofolate cyclo-ligase family; 5-formyltetrahydrofolate cyclo-ligase or methenyl-THF synthetase EC:6.3.3.2 catalyzes the interchange of 5-formyltetrahydrofolate (5-FTHF) to 5-10-methenyltetrahydrofolate, this requires ATP and Mg2+. 5-FTHF is used in chemotherapy where it is clinically known as Leucovorin.


Pssm-ID: 396398 [Multi-domain]  Cd Length: 186  Bit Score: 95.07  E-value: 5.59e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672   81 KQSIRERI---WDYMESHDIADFPRPVHHRIPNFKGSylagQSIRDLEVFagtqeVKVDPDKPLEGVRFLALQSKKTLLV 157
Cdd:pfam01812   1 KQELRKQLlarRRALSEEERAAQSEALHQRLISLPEY----QKAKRVAAY-----VSVGGEIDTRELIDLLLEEGKRVLL 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568956672  158 PTPRLRTGLFN--KITPPPGATKDILRKCATSQGVRNFSVPVGLDSSVLVDLVVVgsvAVSEKGWRIGKGEGYADLEYAM 235
Cdd:pfam01812  72 PVPRPGSGHLDmvRFTPYYPEDSLPRGAWGLKEPVEEELRELALGQLDLVLVPGV---AFDRQGYRLGRGGGYYDRYLAR 148

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 568956672  236 MVSMGAvhKGTPVVTIVHDCQVVDIPealVEDHDLTVDYILTP 278
Cdd:pfam01812 149 LQGHGA--KPYTVGLAFDEQLVERLP---VEPHDVPVDEVVTE 186
FAU1 COG0212
5-formyltetrahydrofolate cyclo-ligase [Coenzyme transport and metabolism];
214-281 6.47e-10

5-formyltetrahydrofolate cyclo-ligase [Coenzyme transport and metabolism];


Pssm-ID: 439982 [Multi-domain]  Cd Length: 186  Bit Score: 57.86  E-value: 6.47e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 568956672 214 AVSEKGWRIGKGEGYAD--LEyammvsmgAVHKGTPVVTIVHDCQVVD-IPealVEDHDLTVDYILTPTRV 281
Cdd:COG0212  127 AFDRRGYRLGYGGGYYDrtLA--------RLRPRPLTIGLAFDCQLVDeLP---VEPHDVPLDAIVTEKGV 186
MTHFS_bact TIGR02727
5,10-methenyltetrahydrofolate synthetase; This enzyme, 5,10-methenyltetrahydrofolate ...
 214-278 1.26e-05

5,10-methenyltetrahydrofolate synthetase; This enzyme, 5,10-methenyltetrahydrofolate synthetase, is also called 5-formyltetrahydrofolate cycloligase. Function of bacterial proteins in this family was inferred originally from the known activity of eukaryotic homologs. Recently, activity was shown explicitly for the member from Mycoplasma pneumonia. Members of this family from alpha- and gamma-proteobacteria, designated ygfA, are often found in an operon with 6S structural RNA, and show a similar pattern of high expression during stationary phase. The function may be to deplete folate to slow 1-carbon biosynthetic metabolism. [Central intermediary metabolism, One-carbon metabolism]


Pssm-ID: 274270 [Multi-domain]  Cd Length: 179  Bit Score: 45.34  E-value: 1.26e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 568956672  214 AVSEKGWRIGKGEGYADLEYAMMVsmgavhkgTPVVTIVHDCQVVD-IPealVEDHDLTVDYILTP 278
Cdd:TIGR02727 125 AFDRRGYRLGYGGGYYDRFLARLK--------GITIGLAFDFQLVDeLP---REPHDVPVDAIITE 179
PLN02812 PLN02812
5-formyltetrahydrofolate cyclo-ligase
 214-282 1.40e-03

5-formyltetrahydrofolate cyclo-ligase


Pssm-ID: 178408  Cd Length: 211  Bit Score: 39.63  E-value: 1.40e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 568956672 214 AVSEKGWRIGKGEGYADLEYAMMVSMgAVHKG--TP-VVTIVHDCQVVDIPEALVEDHDLTVDYILTPTRVI 282
Cdd:PLN02812 141 AFDRSGRRLGRGGGYYDTFLSKYQEL-AKEKGwkQPlLVALSYSPQILDEGSVPVDETDVLVDALVTPSGVI 211
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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