N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like transcription factor 8, KLF8) is a CACCC-box binding protein that associates with C-terminal Binding Protein (CtBP) and represses transcription. It plays an essential role in the regulation of the cell cycle, apoptosis, and differentiation. It has been identified as a key component of the transcription factor network that controls terminal differentiation during adipogenesis. It also plays an important role in the formation of several human tumors, including the promotion of tumorigenesis, invasion, and metastasis of colorectal cancer cells, and the progression of pancreatic cancer. KLF8 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF8 contains an N-terminal repression domain that is related to that of KLF12.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 147 SPSFLAASPMENPALFNDIKIEPPEELLESDFNMPQVEPVDLSFHKPKAPLQPASMLQA-PIRPPKPPTAPQAIMVPTSA 225
Cdd:cd21440     1 SSSLLAASPAETPALLSDIKTEPPEELLASDCSQPQAEPVDLSLHKPKAPLQPPSVLSPsPMILSVSPSAPQSLVSSTGT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 226 DTVTSAAIPTVLTPGSILASSQGTGGQPILHVIHTIPSVSLPNKMSGLKTIPLVVQSLPMVYTSLPTDGSPAAITVPLIG 305
Cdd:cd21440    81 GMGTTSAIPAVLSPGSILASSQGSGGQQILHVIHTIPSVNLPSKMGNLQTIPVVVQSLPVVYTTLPTDGVTAAITVPLIG 160

                  ....*....
gi 1907203850 306 GDGKSAGSV 314
Cdd:cd21440   161 GDGKNAGSV 169

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 1907203850 432 FRCTDCNRSFSRSDHLSLHRRRH 454
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like transcription factor 8, KLF8) is a CACCC-box binding protein that associates with C-terminal Binding Protein (CtBP) and represses transcription. It plays an essential role in the regulation of the cell cycle, apoptosis, and differentiation. It has been identified as a key component of the transcription factor network that controls terminal differentiation during adipogenesis. It also plays an important role in the formation of several human tumors, including the promotion of tumorigenesis, invasion, and metastasis of colorectal cancer cells, and the progression of pancreatic cancer. KLF8 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF8 contains an N-terminal repression domain that is related to that of KLF12.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 147 SPSFLAASPMENPALFNDIKIEPPEELLESDFNMPQVEPVDLSFHKPKAPLQPASMLQA-PIRPPKPPTAPQAIMVPTSA 225
Cdd:cd21440     1 SSSLLAASPAETPALLSDIKTEPPEELLASDCSQPQAEPVDLSLHKPKAPLQPPSVLSPsPMILSVSPSAPQSLVSSTGT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 226 DTVTSAAIPTVLTPGSILASSQGTGGQPILHVIHTIPSVSLPNKMSGLKTIPLVVQSLPMVYTSLPTDGSPAAITVPLIG 305
Cdd:cd21440    81 GMGTTSAIPAVLSPGSILASSQGSGGQQILHVIHTIPSVNLPSKMGNLQTIPVVVQSLPVVYTTLPTDGVTAAITVPLIG 160

                  ....*....
gi 1907203850 306 GDGKSAGSV 314
Cdd:cd21440   161 GDGKNAGSV 169

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*.
gi 1907203850 418 ELTRHFRKHTGIKPFRCTDCNRSFSR 443
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 1907203850 432 FRCTDCNRSFSRSDHLSLHRRRH 454
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

zinc finger;

                           10        20
                   ....*....|....*....|...
gi 1907203850  432 FRCTDCNRSFSRSDHLSLHRRRH 454
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23

N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like transcription factor 8, KLF8) is a CACCC-box binding protein that associates with C-terminal Binding Protein (CtBP) and represses transcription. It plays an essential role in the regulation of the cell cycle, apoptosis, and differentiation. It has been identified as a key component of the transcription factor network that controls terminal differentiation during adipogenesis. It also plays an important role in the formation of several human tumors, including the promotion of tumorigenesis, invasion, and metastasis of colorectal cancer cells, and the progression of pancreatic cancer. KLF8 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF8 contains an N-terminal repression domain that is related to that of KLF12.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 147 SPSFLAASPMENPALFNDIKIEPPEELLESDFNMPQVEPVDLSFHKPKAPLQPASMLQA-PIRPPKPPTAPQAIMVPTSA 225
Cdd:cd21440     1 SSSLLAASPAETPALLSDIKTEPPEELLASDCSQPQAEPVDLSLHKPKAPLQPPSVLSPsPMILSVSPSAPQSLVSSTGT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 226 DTVTSAAIPTVLTPGSILASSQGTGGQPILHVIHTIPSVSLPNKMSGLKTIPLVVQSLPMVYTSLPTDGSPAAITVPLIG 305
Cdd:cd21440    81 GMGTTSAIPAVLSPGSILASSQGSGGQQILHVIHTIPSVNLPSKMGNLQTIPVVVQSLPVVYTTLPTDGVTAAITVPLIG 160

                  ....*....
gi 1907203850 306 GDGKSAGSV 314
Cdd:cd21440   161 GDGKNAGSV 169

N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 159 PALFNDIKIEPPEELLESDFNMPQVEPVDLSFHKPK--------APLQPASMLQAPIRPPKPPTAPQAIMVPTSADTVTS 230
Cdd:cd21441    29 PLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARtsptavssSPVSMTASASPSSSSSSSSSSSRPASSPTVITSVSS 108

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 231 AA-IPTVLTPGSILASSQGTGGQPILHVIHTIPSVS----LPNKMSGLKTIPLVVQSLPMVYTSLPTDGS-PAAITVPLI 304
Cdd:cd21441   109 ASsVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSpmnlQSNKLSHVHRIPVVVQSVPVVYTAVRSPGNvNNTIVVPLL 188

                         170
                  ....*....|
gi 1907203850 305 gGDGKSAGSV 314
Cdd:cd21441   189 -EDGRSHGKA 197

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*.
gi 1907203850 418 ELTRHFRKHTGIKPFRCTDCNRSFSR 443
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26

FOG: Zn-finger [General function prediction only];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 141 DRRNMTSPS--FLAASPMENPALFNDIKIEPPEELLESD-FNMPQVEPVDLSFHKPKAPLQ------PASMLQAPIRPPK 211
Cdd:COG5048    92 DLNSKSLPLsnSKASSSSLSSSSSNSNDNNLLSSHSLPPsSRDPQLPDLLSISNLRNNPLPgnnsssVNTPQSNSLHPPL 171

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 212 P-----PTAPQAIMVPTSADTVTSAAIPTVLTPGSILASSQgtggqpilhviHTIPSVSLPNKMSGLKTIPLVVQSLPMV 286
Cdd:COG5048   172 PanslsKDPSSNLSLLISSNVSTSIPSSSENSPLSSSYSIP-----------SSSSDQNLENSSSSLPLTTNSQLSPKSL 240

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 287 YTSLPTDGSPAAITVPLIGGDGKSAGSVKVDPASmcPLEFPSdSDESAIESGSSALQSLQGFHHEPATMVHMQgEESLDL 366
Cdd:COG5048   241 LSQSPSSLSSSDSSSSASESPRSSLPTASSQSSS--PNESDS-SSEKGFSLPIKSKQCNISFSRSSPLTRHLR-SVNHSG 316

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907203850 367 KRRRIHQCDFAGCSKVYTKSSHLKAHRRIHTGEKPYKCTWDGCSWKF-----ARSDELTRHFRKHTGIKPFRCTD--CNR 439
Cdd:COG5048   317 ESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSETLSnsCIR 396

                         330
                  ....*....|....*...
gi 1907203850 440 SFSRSDHLSLHRRRHDTM 457
Cdd:COG5048   397 NFKRDSNLSLHIITHLSF 414

FOG: Zn-finger [General function prediction only];

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1907203850 400 KPYKCtwDGCSWKFARSDELTRHFRKHTGIKPFRCTDCNRSFSRSDHLSLHRRR 453
Cdd:COG5048    32 RPDSC--PNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHL 83

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*...
gi 1907203850 388 HLKAHRRIHTGEKPYKCtwDGCSWKFAR 415
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKC--PECGKSFKS 26

zinc finger;

                           10        20
                   ....*....|....*....|...
gi 1907203850  432 FRCTDCNRSFSRSDHLSLHRRRH 454
Cdd:smart00355   1 YRCPECGKVFKSKSALREHMRTH 23

C2H2-type zinc finger; This family contains a number of divergent C2H2 type zinc fingers.

                          10        20
                  ....*....|....*....|...
gi 1907203850 432 FRCTDCNRSFSRSDHLSLHRRRH 454
Cdd:pfam13894   1 FKCPICGKSFSSKKSLKRHLKTH 23