mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1 [Mus musculus]
Protein Classification
mitotic checkpoint serine/threonine-protein kinase BUB1; choline kinase family protein( domain architecture ID 13746932)
mitotic checkpoint serine/threonine-protein kinase BUB1 (Budding uninhibited by benzimidazoles 1) catalyzes the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates, and is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment| choline kinase (ChoK) family protein similar to ChoK that catalyzes the transfer of the gamma-phosphoryl group from ATP (or CTP) to its substrate, choline, producing phosphorylcholine (PCho), a precursor to the biosynthesis of two major membrane phospholipids, phosphatidylcholine (PC), and sphingomyelin (SM)
List of domain hits
| Name | Accession | Description | Interval | E-value | |||||
| PKc_like super family | cl21453 | Protein Kinases, catalytic domain; The protein kinase superfamily is mainly composed of the ... |
740-1036 | 1.60e-152 | |||||
Protein Kinases, catalytic domain; The protein kinase superfamily is mainly composed of the catalytic domains of serine/threonine-specific and tyrosine-specific protein kinases. It also includes RIO kinases, which are atypical serine protein kinases, aminoglycoside phosphotransferases, and choline kinases. These proteins catalyze the transfer of the gamma-phosphoryl group from ATP to hydroxyl groups in specific substrates such as serine, threonine, or tyrosine residues of proteins. The actual alignment was detected with superfamily member cd14029: Pssm-ID: 473864 [Multi-domain] Cd Length: 304 Bit Score: 455.09 E-value: 1.60e-152
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| Mad3_BUB1_I | pfam08311 | Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ... |
51-173 | 4.47e-58 | |||||
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p. : Pssm-ID: 462420 Cd Length: 123 Bit Score: 195.44 E-value: 4.47e-58
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| half-pint super family | cl31128 | poly-U binding splicing factor, half-pint family; The proteins represented by this model ... |
253-445 | 7.20e-04 | |||||
poly-U binding splicing factor, half-pint family; The proteins represented by this model contain three RNA recognition motifs (rrm: pfam00076) and have been characterized as poly-pyrimidine tract binding proteins associated with RNA splicing factors. In the case of PUF60 (GP|6176532), in complex with p54, and in the presence of U2AF, facilitates association of U2 snRNP with pre-mRNA. The actual alignment was detected with superfamily member TIGR01645: Pssm-ID: 130706 [Multi-domain] Cd Length: 612 Bit Score: 43.52 E-value: 7.20e-04
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| Name | Accession | Description | Interval | E-value | |||||
| STKc_BubR1_vert | cd14029 | Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint ... |
740-1036 | 1.60e-152 | |||||
Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint protein BubR1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. BubR1 (Budding uninhibited by benzimidazoles R1) is also called Bub1 beta (Bub1b). It contains an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding and a C-terminal kinase domain. It is involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. BubR1 inhibits APC/C through direct binding. It also plays an important role in stabilizing kinetochore-microtubule attachments. Mutant mice expressing only 10% normal BubR1 protein are viable and develop into adult mice, but display many early aging-associated phenotypes including reduced lifespan, muscle atrophy, cataracts, impaired wound healing, and infertility. The BubR1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 270931 [Multi-domain] Cd Length: 304 Bit Score: 455.09 E-value: 1.60e-152
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| Mad3_BUB1_I | pfam08311 | Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ... |
51-173 | 4.47e-58 | |||||
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p. Pssm-ID: 462420 Cd Length: 123 Bit Score: 195.44 E-value: 4.47e-58
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| Mad3_BUB1_I | smart00777 | Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ... |
50-172 | 1.09e-57 | |||||
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p. Pssm-ID: 214817 Cd Length: 124 Bit Score: 194.36 E-value: 1.09e-57
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| PRK14879 | PRK14879 | Kae1-associated kinase Bud32; |
827-911 | 4.76e-04 | |||||
Kae1-associated kinase Bud32; Pssm-ID: 237847 [Multi-domain] Cd Length: 211 Bit Score: 42.59 E-value: 4.76e-04
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| arch_bud32 | TIGR03724 | Kae1-associated kinase Bud32; Members of this protein family are the Bud32 protein associated ... |
827-911 | 5.42e-04 | |||||
Kae1-associated kinase Bud32; Members of this protein family are the Bud32 protein associated with Kae1 (kinase-associated endopeptidase 1) in the Archaea. In many Archaeal genomes, Kae1 and Bud32 are fused. The complex is homologous to the Kae1 and Bud32 subunits of the eukaryotic KEOPS complex, an apparently ancient protein kinase-containing molecular machine. [Unknown function, General] Pssm-ID: 274749 [Multi-domain] Cd Length: 199 Bit Score: 42.20 E-value: 5.42e-04
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| half-pint | TIGR01645 | poly-U binding splicing factor, half-pint family; The proteins represented by this model ... |
253-445 | 7.20e-04 | |||||
poly-U binding splicing factor, half-pint family; The proteins represented by this model contain three RNA recognition motifs (rrm: pfam00076) and have been characterized as poly-pyrimidine tract binding proteins associated with RNA splicing factors. In the case of PUF60 (GP|6176532), in complex with p54, and in the presence of U2AF, facilitates association of U2 snRNP with pre-mRNA. Pssm-ID: 130706 [Multi-domain] Cd Length: 612 Bit Score: 43.52 E-value: 7.20e-04
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| SPS1 | COG0515 | Serine/threonine protein kinase [Signal transduction mechanisms]; |
827-903 | 2.91e-03 | |||||
Serine/threonine protein kinase [Signal transduction mechanisms]; Pssm-ID: 440281 [Multi-domain] Cd Length: 482 Bit Score: 41.54 E-value: 2.91e-03
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| Name | Accession | Description | Interval | E-value | |||||
| STKc_BubR1_vert | cd14029 | Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint ... |
740-1036 | 1.60e-152 | |||||
Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint protein BubR1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. BubR1 (Budding uninhibited by benzimidazoles R1) is also called Bub1 beta (Bub1b). It contains an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding and a C-terminal kinase domain. It is involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. BubR1 inhibits APC/C through direct binding. It also plays an important role in stabilizing kinetochore-microtubule attachments. Mutant mice expressing only 10% normal BubR1 protein are viable and develop into adult mice, but display many early aging-associated phenotypes including reduced lifespan, muscle atrophy, cataracts, impaired wound healing, and infertility. The BubR1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 270931 [Multi-domain] Cd Length: 304 Bit Score: 455.09 E-value: 1.60e-152
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| Mad3_BUB1_I | pfam08311 | Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ... |
51-173 | 4.47e-58 | |||||
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p. Pssm-ID: 462420 Cd Length: 123 Bit Score: 195.44 E-value: 4.47e-58
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| Mad3_BUB1_I | smart00777 | Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ... |
50-172 | 1.09e-57 | |||||
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p. Pssm-ID: 214817 Cd Length: 124 Bit Score: 194.36 E-value: 1.09e-57
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| STKc_Bub1_BubR1 | cd13981 | Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 ... |
784-1031 | 1.49e-33 | |||||
Catalytic domain of the Serine/Threonine kinases, Spindle assembly checkpoint proteins Bub1 and BubR1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily is composed of Bub1 (Budding uninhibited by benzimidazoles 1), BubR1, and similar proteins. They contain an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding and a C-terminal kinase domain. Bub1 and BubR1 are involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. Impaired SAC leads to genomic instabilities and tumor development. Bub1 and BubR1 facilitate the localization of SAC proteins to kinetochores and regulate kinetochore-microtubule (K-MT) attachments. Repression studies of Bub1 and BubR1 show that they exert an additive effect in misalignment phenotypes and may function cooperatively or in parallel pathways in regulating K-MT attachments. The Bub1/BubR1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 270883 [Multi-domain] Cd Length: 298 Bit Score: 131.32 E-value: 1.49e-33
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| STKc_Bub1_vert | cd14028 | Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint ... |
792-1006 | 1.55e-22 | |||||
Catalytic domain of the Serine/Threonine kinase, Vertebrate Spindle assembly checkpoint protein Bub1; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Bub1 (Budding uninhibited by benzimidazoles 1) contains an N-terminal Bub1/Mad3 homology domain essential for Cdc20 binding, a GLEBS motif for Bub3/kinetochore binding, and a C-terminal kinase domain. It is involved in SAC, a surveillance system that delays metaphase to anaphase transition by blocking the activity of APC/C (the anaphase promoting complex) until all chromosomes achieve proper attachments to the mitotic spindle, to avoid chromosome missegregation. Bub1 contributes to the inhibition of APC/C by phosphorylating its crucial cofactor, Cdc20, rendering it unable to activate APC/C. In addition, Bub1 facilitates the localization to kinetochores of other SAC and motor proteins including Mad1, Mad2, BubR1, and Plk1. It acts as the master organizer of the functional inner centromere. Bub1 also play roles in protecting sister chromatid cohesion and normal metaphase congression. The Bub1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 270930 [Multi-domain] Cd Length: 290 Bit Score: 99.15 E-value: 1.55e-22
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| STKc_IRAK2 | cd14157 | Catalytic domain of the Serine/Threonine kinase, Interleukin-1 Receptor Associated Kinase 2; ... |
811-945 | 4.42e-05 | |||||
Catalytic domain of the Serine/Threonine kinase, Interleukin-1 Receptor Associated Kinase 2; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. IRAKs are involved in Toll-like receptor (TLR) and interleukin-1 (IL-1) signalling pathways, and are thus critical in regulating innate immune responses and inflammation. IRAKs contain an N-terminal Death domain (DD), a proST region (rich in serines, prolines, and threonines), a central kinase domain, and a C-terminal domain; IRAK-4 lacks the C-terminal domain. Vertebrates contain four IRAKs (IRAK-1, -2, -3 (or -M), and -4) that display distinct functions and patterns of expression and subcellular distribution, and can differentially mediate TLR signaling. IRAK2 plays a role in mediating NFkB activation by TLR3, TLR4, and TLR8. It is specifically targeted by the viral protein A52, which is important for virulence, to inhibit all IL-1/TLR pathways, indicating that IRAK2 has a predominant role in NFkB activation. It is redundant with IRAK1 in early signaling but is critical for late and sustained activation. The IRAK2 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 271059 [Multi-domain] Cd Length: 289 Bit Score: 46.75 E-value: 4.42e-05
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| PRK14879 | PRK14879 | Kae1-associated kinase Bud32; |
827-911 | 4.76e-04 | |||||
Kae1-associated kinase Bud32; Pssm-ID: 237847 [Multi-domain] Cd Length: 211 Bit Score: 42.59 E-value: 4.76e-04
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| STKc_PhKG1 | cd14182 | Catalytic domain of the Serine/Threonine Kinase, Phosphorylase kinase Gamma 1 subunit; STKs ... |
852-939 | 5.22e-04 | |||||
Catalytic domain of the Serine/Threonine Kinase, Phosphorylase kinase Gamma 1 subunit; STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Phosphorylase kinase (PhK) catalyzes the phosphorylation of inactive phosphorylase b to form the active phosphorylase a. It coordinates hormonal, metabolic, and neuronal signals to initiate the breakdown of glycogen stores, which enables the maintenance of blood-glucose homeostasis during fasting, and is also used as a source of energy for muscle contraction. PhK is one of the largest and most complex protein kinases, composed of a heterotetramer containing four molecules each of four subunit types: one catalytic (gamma) and three regulatory (alpha, beta, and delta). The gamma 1 subunit (PhKG1) is also referred to as the muscle gamma isoform. The gamma subunit, when isolated, is constitutively active and does not require phosphorylation of the A-loop for activity. The regulatory subunits restrain this kinase activity until signals are received to relieve this inhibition. For example, the kinase is activated in response to hormonal stimulation, after autophosphorylation or phosphorylation by cAMP-dependent kinase of the alpha and beta subunits. The high-affinity binding of ADP to the beta subunit also stimulates kinase activity, whereas calcium relieves inhibition by binding to the delta (calmodulin) subunit. The PhKG1 subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 271084 [Multi-domain] Cd Length: 276 Bit Score: 43.37 E-value: 5.22e-04
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| arch_bud32 | TIGR03724 | Kae1-associated kinase Bud32; Members of this protein family are the Bud32 protein associated ... |
827-911 | 5.42e-04 | |||||
Kae1-associated kinase Bud32; Members of this protein family are the Bud32 protein associated with Kae1 (kinase-associated endopeptidase 1) in the Archaea. In many Archaeal genomes, Kae1 and Bud32 are fused. The complex is homologous to the Kae1 and Bud32 subunits of the eukaryotic KEOPS complex, an apparently ancient protein kinase-containing molecular machine. [Unknown function, General] Pssm-ID: 274749 [Multi-domain] Cd Length: 199 Bit Score: 42.20 E-value: 5.42e-04
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| half-pint | TIGR01645 | poly-U binding splicing factor, half-pint family; The proteins represented by this model ... |
253-445 | 7.20e-04 | |||||
poly-U binding splicing factor, half-pint family; The proteins represented by this model contain three RNA recognition motifs (rrm: pfam00076) and have been characterized as poly-pyrimidine tract binding proteins associated with RNA splicing factors. In the case of PUF60 (GP|6176532), in complex with p54, and in the presence of U2AF, facilitates association of U2 snRNP with pre-mRNA. Pssm-ID: 130706 [Multi-domain] Cd Length: 612 Bit Score: 43.52 E-value: 7.20e-04
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| SPS1 | COG0515 | Serine/threonine protein kinase [Signal transduction mechanisms]; |
827-903 | 2.91e-03 | |||||
Serine/threonine protein kinase [Signal transduction mechanisms]; Pssm-ID: 440281 [Multi-domain] Cd Length: 482 Bit Score: 41.54 E-value: 2.91e-03
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| PKc | cd00180 | Catalytic domain of Protein Kinases; PKs catalyze the transfer of the gamma-phosphoryl group ... |
811-909 | 6.47e-03 | |||||
Catalytic domain of Protein Kinases; PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. PKs make up a large family of serine/threonine kinases (STKs), protein tyrosine kinases (PTKs), and dual-specificity PKs that phosphorylate both serine/threonine and tyrosine residues of target proteins. Majority of protein phosphorylation occurs on serine residues while only 1% occurs on tyrosine residues. Protein phosphorylation is a mechanism by which a wide variety of cellular proteins, such as enzymes and membrane channels, are reversibly regulated in response to certain stimuli. PKs often function as components of signal transduction pathways in which one kinase activates a second kinase, which in turn, may act on other kinases; this sequential action transmits a signal from the cell surface to target proteins, which results in cellular responses. The PK family is one of the largest known protein families with more than 100 homologous yeast enzymes and more than 500 human proteins. A fraction of PK family members are pseudokinases that lack crucial residues for catalytic activity. The mutiplicity of kinases allows for specific regulation according to substrate, tissue distribution, and cellular localization. PKs regulate many cellular processes including proliferation, division, differentiation, motility, survival, metabolism, cell-cycle progression, cytoskeletal rearrangement, immunity, and neuronal functions. Many kinases are implicated in the development of various human diseases including different types of cancer. The PK family is part of a larger superfamily that includes the catalytic domains of RIO kinases, aminoglycoside phosphotransferase, choline kinase, phosphoinositide 3-kinase (PI3K), and actin-fragmin kinase. Pssm-ID: 270622 [Multi-domain] Cd Length: 215 Bit Score: 39.18 E-value: 6.47e-03
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| PK_eIF2AK_GCN2_rpt1 | cd14012 | Pseudokinase domain, repeat 1, of eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or ... |
822-910 | 7.17e-03 | |||||
Pseudokinase domain, repeat 1, of eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or General Control Non-derepressible-2; The pseudokinase domain shows similarity to protein kinases but lacks crucial residues for catalytic activity. EIF2AKs phosphorylate the alpha subunit of eIF-2, resulting in the overall downregulation of protein synthesis. eIF-2 phosphorylation is induced in response to cellular stresses including virus infection, heat shock, nutrient deficiency, and the accummulation of unfolded proteins, among others. There are four distinct kinases that phosphorylate eIF-2 and control protein synthesis under different stress conditions: GCN2, protein kinase regulated by RNA (PKR), heme-regulated inhibitor kinase (HRI), and PKR-like endoplasmic reticulum kinase (PERK). GCN2 is activated by amino acid or serum starvation and UV irradiation. It induces GCN4, a transcriptional activator of amino acid biosynthetic genes, leading to increased production of amino acids under amino acid-deficient conditions. In serum-starved cells, GCN2 activation induces translation of the stress-responsive transcription factor ATF4, while under UV stress, GCN2 triggers transcriptional rescue via NF-kappaB signaling. GCN2 contains an N-terminal RWD, a degenerate kinase-like (repeat 1), the catalytic kinase (repeat 2), a histidyl-tRNA synthetase (HisRS)-like, and a C-terminal ribosome-binding and dimerization (RB/DD) domains. The degenerate pseudokinase domain of GCN2 may function as a regulatory domain. The GCN2 subfamily is part of a larger superfamily that includes the catalytic domains of serine/threonine kinases, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Pssm-ID: 270914 [Multi-domain] Cd Length: 254 Bit Score: 39.65 E-value: 7.17e-03
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