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Conserved domains on  [gi|530395059|ref|XP_005252844|]
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xylosyl- and glucuronyltransferase LARGE2 isoform X16 [Homo sapiens]

Protein Classification

LARGE family glycosyltransferase( domain architecture ID 82316)

LARGE family glycosyltransferase is a bifunctional glycosyltransferase containing N-terminal family 8 and C-terminal family 49 glycosyltransferase domains, similar to LARGE xylosyl- and glucuronyltransferase proteins, which exhibit both alpha-1,3-xylosyltransferase and beta-1,3-glucuronyltransferase activities and are involved in the maturation of alpha-dystroglycan

CAZY:  GT8|GT49
EC:  2.4.-.-
Gene Ontology:  GO:0016757|GO:0006486
PubMed:  9892679|15958417|12691742|10521532|9445404
SCOP:  3000077

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Glyco_tranf_GTA_type super family cl11394
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 54-136 2.19e-48

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


The actual alignment was detected with superfamily member cd06431:

Pssm-ID: 472172  Cd Length: 280  Bit Score: 168.04  E-value: 2.19e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  54 QDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFYLTFLEYDGNLLRR 133
Cdd:cd06431  198 QDIFNAVIKQNPFLVYQLPCAWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRR 277


                 ...
gi 530395059 134 ELF 136
Cdd:cd06431  278 ELF 280
Glyco_transf_49 super family cl16461
Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the ...
 191-439 4.58e-48

Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the glycosylation of the alpha-dystroglycan subunit. Dystroglycan is an integral member of the skeletal muscular dystrophin glycoprotein complex, which links dystrophin to proteins in the extracellular matrix.


The actual alignment was detected with superfamily member pfam13896:

Pssm-ID: 464027  Cd Length: 327  Bit Score: 168.58  E-value: 4.58e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  191 DVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEA----SPVLAARQDVAYHVVYREGP--------- 257
Cdd:pfam13896   1 DVTLATHGTVDFLDNLEPLVERWRGPISVAVFAPGTDFSLALDYIAYlrrcFPSELVRENVTFHLVFPSEHmppkqvtcp 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  258 --------------------------------LYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLR--ASIEQLGL 303
Cdd:pfam13896  81 sallsssndcsellsplrklvppganyaaqnlLYPINLLRNVARKGAQTHFVLVIDIDLYPSPGLAEKFLefLARNKKLL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  304 GSRRKAALVVPAFEtLRYRFSFPHSKVELLALLDAGTLYTFrYHEW-PRGHAPTDYARWREAQAP---------YRV-QW 372
Cdd:pfam13896 161 NRTSPCVFVVPAFE-VDANATVPRTKAELLRLLKNGEARPF-HHKVcPKCHKPTNYDRWLNLSKNsdglnlfvaYKVtYW 238

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 530395059  373 AANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVEldaqarqarqpllLCptLQEYELLVLPEAFTIH 439
Cdd:pfam13896 239 QDPWEPFYIGTRNDPLYDERFTWYGFDRISQVYE-------------LC--VAGYEFHVLDNAFLVH 290
 
Name Accession Description Interval E-value
GT8_LARGE_C cd06431
LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in ...
 54-136 2.19e-48

LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis; The catalytic domain of LARGE is a putative glycosyltransferase. Mutations of LARGE in mouse and human cause dystroglycanopathies, a disease associated with hypoglycosylation of the membrane protein alpha-dystroglycan (alpha-DG) and consequent loss of extracellular ligand binding. LARGE needs to both physically interact with alpha-dystroglycan and function as a glycosyltransferase in order to stimulate alpha-dystroglycan hyperglycosylation. LARGE localizes to the Golgi apparatus and contains three conserved DxD motifs. While two of the motifs are indispensible for glycosylation function, one is important for localization of th eenzyme. LARGE was originally named because it covers approximately large trunck of genomic DNA, more than 600bp long. The predicted protein structure contains an N-terminal cytoplasmic domain, a transmembrane region, a coiled-coil motif, and two putative catalytic domains. This catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis.


Pssm-ID: 133053  Cd Length: 280  Bit Score: 168.04  E-value: 2.19e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  54 QDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFYLTFLEYDGNLLRR 133
Cdd:cd06431  198 QDIFNAVIKQNPFLVYQLPCAWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRR 277


                 ...
gi 530395059 134 ELF 136
Cdd:cd06431  278 ELF 280
Glyco_transf_49 pfam13896
Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the ...
 191-439 4.58e-48

Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the glycosylation of the alpha-dystroglycan subunit. Dystroglycan is an integral member of the skeletal muscular dystrophin glycoprotein complex, which links dystrophin to proteins in the extracellular matrix.


Pssm-ID: 464027  Cd Length: 327  Bit Score: 168.58  E-value: 4.58e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  191 DVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEA----SPVLAARQDVAYHVVYREGP--------- 257
Cdd:pfam13896   1 DVTLATHGTVDFLDNLEPLVERWRGPISVAVFAPGTDFSLALDYIAYlrrcFPSELVRENVTFHLVFPSEHmppkqvtcp 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  258 --------------------------------LYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLR--ASIEQLGL 303
Cdd:pfam13896  81 sallsssndcsellsplrklvppganyaaqnlLYPINLLRNVARKGAQTHFVLVIDIDLYPSPGLAEKFLefLARNKKLL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  304 GSRRKAALVVPAFEtLRYRFSFPHSKVELLALLDAGTLYTFrYHEW-PRGHAPTDYARWREAQAP---------YRV-QW 372
Cdd:pfam13896 161 NRTSPCVFVVPAFE-VDANATVPRTKAELLRLLKNGEARPF-HHKVcPKCHKPTNYDRWLNLSKNsdglnlfvaYKVtYW 238

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 530395059  373 AANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVEldaqarqarqpllLCptLQEYELLVLPEAFTIH 439
Cdd:pfam13896 239 QDPWEPFYIGTRNDPLYDERFTWYGFDRISQVYE-------------LC--VAGYEFHVLDNAFLVH 290
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
 52-105 1.76e-03

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 40.00  E-value: 1.76e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 530395059   52 LTQDIFNAVIKehpGLVQRLPCVWNVQLSDHTLAERCYSEASD-LKVIHWNSPKK 105
Cdd:pfam01501 195 GDQDPLNIVFY---GKVKPLDPRWNVLGLGYYNKKKSLNEITEnAAVIHYNGPTK 246
 
Name Accession Description Interval E-value
GT8_LARGE_C cd06431
LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in ...
 54-136 2.19e-48

LARGE catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis; The catalytic domain of LARGE is a putative glycosyltransferase. Mutations of LARGE in mouse and human cause dystroglycanopathies, a disease associated with hypoglycosylation of the membrane protein alpha-dystroglycan (alpha-DG) and consequent loss of extracellular ligand binding. LARGE needs to both physically interact with alpha-dystroglycan and function as a glycosyltransferase in order to stimulate alpha-dystroglycan hyperglycosylation. LARGE localizes to the Golgi apparatus and contains three conserved DxD motifs. While two of the motifs are indispensible for glycosylation function, one is important for localization of th eenzyme. LARGE was originally named because it covers approximately large trunck of genomic DNA, more than 600bp long. The predicted protein structure contains an N-terminal cytoplasmic domain, a transmembrane region, a coiled-coil motif, and two putative catalytic domains. This catalytic domain has closest homology to GT8 glycosyltransferase involved in lipooligosaccharide synthesis.


Pssm-ID: 133053  Cd Length: 280  Bit Score: 168.04  E-value: 2.19e-48
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  54 QDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFYLTFLEYDGNLLRR 133
Cdd:cd06431  198 QDIFNAVIKQNPFLVYQLPCAWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRR 277


                 ...
gi 530395059 134 ELF 136
Cdd:cd06431  278 ELF 280
Glyco_transf_49 pfam13896
Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the ...
 191-439 4.58e-48

Glycosyl-transferase for dystroglycan; This glycosyl-transferase brings about the glycosylation of the alpha-dystroglycan subunit. Dystroglycan is an integral member of the skeletal muscular dystrophin glycoprotein complex, which links dystrophin to proteins in the extracellular matrix.


Pssm-ID: 464027  Cd Length: 327  Bit Score: 168.58  E-value: 4.58e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  191 DVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEA----SPVLAARQDVAYHVVYREGP--------- 257
Cdd:pfam13896   1 DVTLATHGTVDFLDNLEPLVERWRGPISVAVFAPGTDFSLALDYIAYlrrcFPSELVRENVTFHLVFPSEHmppkqvtcp 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  258 --------------------------------LYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLR--ASIEQLGL 303
Cdd:pfam13896  81 sallsssndcsellsplrklvppganyaaqnlLYPINLLRNVARKGAQTHFVLVIDIDLYPSPGLAEKFLefLARNKKLL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  304 GSRRKAALVVPAFEtLRYRFSFPHSKVELLALLDAGTLYTFrYHEW-PRGHAPTDYARWREAQAP---------YRV-QW 372
Cdd:pfam13896 161 NRTSPCVFVVPAFE-VDANATVPRTKAELLRLLKNGEARPF-HHKVcPKCHKPTNYDRWLNLSKNsdglnlfvaYKVtYW 238

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 530395059  373 AANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVEldaqarqarqpllLCptLQEYELLVLPEAFTIH 439
Cdd:pfam13896 239 QDPWEPFYIGTRNDPLYDERFTWYGFDRISQVYE-------------LC--VAGYEFHVLDNAFLVH 290
Glyco_transf_8 cd00505
Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis ...
 51-109 9.58e-12

Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis; Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+.


Pssm-ID: 132996 [Multi-domain]  Cd Length: 246  Bit Score: 65.16  E-value: 9.58e-12
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 530395059  51 DLTQDIFNAVIKEHPGLVQRLPCVWNVQLSDHTLAERC-YSEASDLKVIHWNSPKKLRVK 109
Cdd:cd00505  187 GGDQDLLNTFFKQVPFIVKSLPCIWNVRLTGCYRSLNCfKAFVKNAKVIHFNGPTKPWNK 246
Glyco_transf_8 pfam01501
Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to ...
 52-105 1.76e-03

Glycosyl transferase family 8; This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase.


Pssm-ID: 279798 [Multi-domain]  Cd Length: 252  Bit Score: 40.00  E-value: 1.76e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 530395059   52 LTQDIFNAVIKehpGLVQRLPCVWNVQLSDHTLAERCYSEASD-LKVIHWNSPKK 105
Cdd:pfam01501 195 GDQDPLNIVFY---GKVKPLDPRWNVLGLGYYNKKKSLNEITEnAAVIHYNGPTK 246
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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