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Conserved domains on  [gi|445980965|ref|WP_000058820|]
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MULTISPECIES: Fe-S cluster assembly sulfur transfer protein SufU [Leptospira]

Protein Classification

iron-sulfur cluster assembly scaffold protein( domain architecture ID 10797689)

iron-sulfur cluster assembly scaffold protein, such as Escherichia coli IscU, on which IscS assembles Fe-S clusters

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SUF_scaf_2 TIGR01994
SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are ...
 5-139 1.84e-75

SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are known so far. ISC is broadly distributed while NIF tends to be associated with nitrogenase in nitrogen-fixing bacteria. The most recently described is SUF, believed to be important to maintain the function during aerobic stress of enzymes with labile Fe-S clusters. It is fairly widely distributed. This family represents one of two different proteins proposed to act as a scaffold on which the Fe-S cluster is built and from which it is transferred. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


:

Pssm-ID: 273918  Cd Length: 137  Bit Score: 220.66  E-value: 1.84e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    5 DSLYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIA 84
Cdd:TIGR01994   2 DSLYRQVILDHYKNPRHRGKLEDATVQERGHNPTCGDEITLTVKLEGDRIEDIAFEGEGCSISQASASMMTELIKGKTVE 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 445980965   85 EAKDIILRFKNMILEDQDPKFDEGLEDLESMESVKKIPARIKCAVLPWNTLERAF 139
Cdd:TIGR01994  82 EALSLVEAFSEMIQGQETDEDEEKLGDAEALAGVAKFPARIKCATLAWKALERAL 136
 
Name Accession Description Interval E-value
SUF_scaf_2 TIGR01994
SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are ...
 5-139 1.84e-75

SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are known so far. ISC is broadly distributed while NIF tends to be associated with nitrogenase in nitrogen-fixing bacteria. The most recently described is SUF, believed to be important to maintain the function during aerobic stress of enzymes with labile Fe-S clusters. It is fairly widely distributed. This family represents one of two different proteins proposed to act as a scaffold on which the Fe-S cluster is built and from which it is transferred. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 273918  Cd Length: 137  Bit Score: 220.66  E-value: 1.84e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    5 DSLYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIA 84
Cdd:TIGR01994   2 DSLYRQVILDHYKNPRHRGKLEDATVQERGHNPTCGDEITLTVKLEGDRIEDIAFEGEGCSISQASASMMTELIKGKTVE 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 445980965   85 EAKDIILRFKNMILEDQDPKFDEGLEDLESMESVKKIPARIKCAVLPWNTLERAF 139
Cdd:TIGR01994  82 EALSLVEAFSEMIQGQETDEDEEKLGDAEALAGVAKFPARIKCATLAWKALERAL 136
IscU COG0822
Fe-S cluster assembly scaffold protein IscU, NifU family [Posttranslational modification, ...
 5-141 1.63e-53

Fe-S cluster assembly scaffold protein IscU, NifU family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440584  Cd Length: 128  Bit Score: 164.63  E-value: 1.63e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965   5 DSLYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIA 84
Cdd:COG0822    3 DDLYSEKILDHAKNPRNVGELEDADGSGEGGNPLCGDTVTLYLKVDDGRIEDAKFEGFGCAISQASASMLTELVKGKTLE 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 445980965  85 EAKDIILRFknmiledqdpkfdeglEDLESMESVKKIPARIKCAVLPWNTLERAFLQ 141
Cdd:COG0822   83 EALALTDEF----------------GDLAALGGLPKFPARVKCALLAWDALKAALAD 123
IscU_like cd06664
Iron-sulfur cluster scaffold-like proteins; IscU_like and NifU_like proteins. IscU and NifU ...
 7-138 1.02e-50

Iron-sulfur cluster scaffold-like proteins; IscU_like and NifU_like proteins. IscU and NifU function as a scaffold for the assembly of [2Fe-2S] clusters before they are transferred to apo target proteins. They are highly conserved and play vital roles in the ISC and NIF systems of Fe-S protein maturation. NIF genes participate in nitrogen fixation in several isolated bacterial species. The NifU domain, however, is also found in bacteria that do not fix nitrogen, so it may have wider significance in the cell. Human IscU interacts with frataxin, the Friedreich ataxia gene product, and incorrectly spliced IscU has been shown to disrupt iron homeostasis in skeletal muscle and cause myopathy.


Pssm-ID: 143480  Cd Length: 123  Bit Score: 157.41  E-value: 1.02e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965   7 LYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIAEA 86
Cdd:cd06664    1 LYSEIILDHYRNPRNVGRLEDADGTGEVGNPLCGDEITLYLKVEDGRITDAKFQGFGCAISIASASLLTELIKGKTLDEA 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 445980965  87 KDIILRFKNMIledqdpkfdEGLEDLESMESVKKIPARIKCAVLPWNTLERA 138
Cdd:cd06664   81 LKLLNKDIAML---------DGKEELAALAGVGLPPARIHCALLAWKALKAA 123
NifU_N pfam01592
NifU-like N terminal domain; This domain is found in NifU in combination with pfam01106. This ...
 7-138 3.22e-27

NifU-like N terminal domain; This domain is found in NifU in combination with pfam01106. This domain is found on isolated in several bacterial species. The nif genes are responsible for nitrogen fixation. However this domain is found in bacteria that do not fix nitrogen, so it may have a broader significance in the cell than nitrogen fixation. These proteins appear to be scaffold proteins for iron-sulfur clusters.


Pssm-ID: 426336  Cd Length: 127  Bit Score: 98.25  E-value: 3.22e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    7 LYKEVILDHYQNPRFRGKLESADLY--EHGiNPLCGDELELTINLDGN--RITEIRVTGKGCSISQASGSMMAESIYGKT 82
Cdd:pfam01592   1 LYTDKVLDHYKNPRNVGVLEDADAGvgDVG-NPACGDAMRLQIKVDEStdRIEDAKFKTFGCGSAIASSSMLTELVKGKT 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 445980965   83 IAEAKDIilrfknmiledqdpKFDEGLEDLESMESVKkiparIKCAVLPWNTLERA 138
Cdd:pfam01592  80 IEEALKI--------------TNTDIAEELGGLPPVK-----MHCSVLADDALKAA 116
 
Name Accession Description Interval E-value
SUF_scaf_2 TIGR01994
SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are ...
 5-139 1.84e-75

SUF system FeS assembly protein, NifU family; Three iron-sulfur cluster assembly systems are known so far. ISC is broadly distributed while NIF tends to be associated with nitrogenase in nitrogen-fixing bacteria. The most recently described is SUF, believed to be important to maintain the function during aerobic stress of enzymes with labile Fe-S clusters. It is fairly widely distributed. This family represents one of two different proteins proposed to act as a scaffold on which the Fe-S cluster is built and from which it is transferred. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 273918  Cd Length: 137  Bit Score: 220.66  E-value: 1.84e-75
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    5 DSLYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIA 84
Cdd:TIGR01994   2 DSLYRQVILDHYKNPRHRGKLEDATVQERGHNPTCGDEITLTVKLEGDRIEDIAFEGEGCSISQASASMMTELIKGKTVE 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 445980965   85 EAKDIILRFKNMILEDQDPKFDEGLEDLESMESVKKIPARIKCAVLPWNTLERAF 139
Cdd:TIGR01994  82 EALSLVEAFSEMIQGQETDEDEEKLGDAEALAGVAKFPARIKCATLAWKALERAL 136
IscU COG0822
Fe-S cluster assembly scaffold protein IscU, NifU family [Posttranslational modification, ...
 5-141 1.63e-53

Fe-S cluster assembly scaffold protein IscU, NifU family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440584  Cd Length: 128  Bit Score: 164.63  E-value: 1.63e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965   5 DSLYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIA 84
Cdd:COG0822    3 DDLYSEKILDHAKNPRNVGELEDADGSGEGGNPLCGDTVTLYLKVDDGRIEDAKFEGFGCAISQASASMLTELVKGKTLE 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 445980965  85 EAKDIILRFknmiledqdpkfdeglEDLESMESVKKIPARIKCAVLPWNTLERAFLQ 141
Cdd:COG0822   83 EALALTDEF----------------GDLAALGGLPKFPARVKCALLAWDALKAALAD 123
IscU_like cd06664
Iron-sulfur cluster scaffold-like proteins; IscU_like and NifU_like proteins. IscU and NifU ...
 7-138 1.02e-50

Iron-sulfur cluster scaffold-like proteins; IscU_like and NifU_like proteins. IscU and NifU function as a scaffold for the assembly of [2Fe-2S] clusters before they are transferred to apo target proteins. They are highly conserved and play vital roles in the ISC and NIF systems of Fe-S protein maturation. NIF genes participate in nitrogen fixation in several isolated bacterial species. The NifU domain, however, is also found in bacteria that do not fix nitrogen, so it may have wider significance in the cell. Human IscU interacts with frataxin, the Friedreich ataxia gene product, and incorrectly spliced IscU has been shown to disrupt iron homeostasis in skeletal muscle and cause myopathy.


Pssm-ID: 143480  Cd Length: 123  Bit Score: 157.41  E-value: 1.02e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965   7 LYKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIAEA 86
Cdd:cd06664    1 LYSEIILDHYRNPRNVGRLEDADGTGEVGNPLCGDEITLYLKVEDGRITDAKFQGFGCAISIASASLLTELIKGKTLDEA 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 445980965  87 KDIILRFKNMIledqdpkfdEGLEDLESMESVKKIPARIKCAVLPWNTLERA 138
Cdd:cd06664   81 LKLLNKDIAML---------DGKEELAALAGVGLPPARIHCALLAWKALKAA 123
NifU_N pfam01592
NifU-like N terminal domain; This domain is found in NifU in combination with pfam01106. This ...
 7-138 3.22e-27

NifU-like N terminal domain; This domain is found in NifU in combination with pfam01106. This domain is found on isolated in several bacterial species. The nif genes are responsible for nitrogen fixation. However this domain is found in bacteria that do not fix nitrogen, so it may have a broader significance in the cell than nitrogen fixation. These proteins appear to be scaffold proteins for iron-sulfur clusters.


Pssm-ID: 426336  Cd Length: 127  Bit Score: 98.25  E-value: 3.22e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    7 LYKEVILDHYQNPRFRGKLESADLY--EHGiNPLCGDELELTINLDGN--RITEIRVTGKGCSISQASGSMMAESIYGKT 82
Cdd:pfam01592   1 LYTDKVLDHYKNPRNVGVLEDADAGvgDVG-NPACGDAMRLQIKVDEStdRIEDAKFKTFGCGSAIASSSMLTELVKGKT 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 445980965   83 IAEAKDIilrfknmiledqdpKFDEGLEDLESMESVKkiparIKCAVLPWNTLERA 138
Cdd:pfam01592  80 IEEALKI--------------TNTDIAEELGGLPPVK-----MHCSVLADDALKAA 116
NifU_clost TIGR03419
FeS cluster assembly scaffold protein NifU, Clostridium type; NifU and NifS form a pair of ...
 8-89 3.44e-17

FeS cluster assembly scaffold protein NifU, Clostridium type; NifU and NifS form a pair of iron-sulfur (FeS) cluster biosynthesis proteins much simpler than the ISC and SUF systems. Members of this protein family are a distinct group of NifU-like proteins, found always to a NifS-like protein and restricted to species that lack a SUF system. Typically, NIF systems service a smaller number of FeS-containing proteins than do ISC or SUF. Members of this particular branch typically are found, almost half the time, near the mnmA gene, involved in the carboxymethylaminomethyl modification of U34 in some tRNAs (see GenProp0704). While other NifU proteins are associated with nitrogen fixation, this family is not. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 132460  Cd Length: 121  Bit Score: 72.08  E-value: 3.44e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 445980965    8 YKEVILDHYQNPRFRGKLESADLYEHGINPLCGDELELTINLDGNRITEIRVTGKGCSISQASGSMMAESIYGKTIAEAK 87
Cdd:TIGR03419   1 YSEKVMDHFMNPRNVGEIENADGVGEVGNPKCGDIMKIFLKVEDDIIKDVKFKTFGCGAAIASSSMATEMIKGKTLEEAW 80


                  ..
gi 445980965   88 DI 89
Cdd:TIGR03419  81 EL 82
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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