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Conserved domains on  [gi|46358344|ref|NP_957245|]
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isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Danio rerio]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
29-361 0e+00

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 557.77  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    29 GIQTVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGpgGRWMIPPEAKESMDKNKIGLKGPLKTPIAAG-HP 107
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTD--GKTEIPDEAVESIKRNKVALKGPLETPIGKGgHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   108 SMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYAR 187
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   188 NNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIG 267
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   268 GLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDLGGN 347
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 46358344   348 SKCSEFTADICRRV 361
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 29-361 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 557.77  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    29 GIQTVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGpgGRWMIPPEAKESMDKNKIGLKGPLKTPIAAG-HP 107
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTD--GKTEIPDEAVESIKRNKVALKGPLETPIGKGgHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   108 SMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYAR 187
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   188 NNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIG 267
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   268 GLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDLGGN 347
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 46358344   348 SKCSEFTADICRRV 361
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 7-358 6.93e-170

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 478.22  E-value: 6.93e-170
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    7 SAVSCIMGAIKTQTPQPRVFSRGIQ------TVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGPGGRWMIP 80
Cdd:PLN00118  12 NRLAQILGASSSSSGAFSSSARAFSssstpiTATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTGSFLT 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   81 PEAKESMDKNKIGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIV 160
Cdd:PLN00118  92 WESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVV 171

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  161 DGVVQSIKLITEEASRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQ 240
Cdd:PLN00118 172 RGVVESLKIITRQASLRVAEYAFHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVK 251

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  241 DPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLH 320
Cdd:PLN00118 252 NPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLKLN 331

                        330       340       350
                 ....*....|....*....|....*....|....*...
gi 46358344  321 GHAKKIETACFDTIRDKKVLTKDLGGNSKCSEFTADIC 358
Cdd:PLN00118 332 EQAEQIHNAILNTIAEGKYRTADLGGSSTTTDFTKAIC 369
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 32-361 4.41e-153

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 434.44  E-value: 4.41e-153
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  32 TVTLIPGDGIGPEISTAVMKIFEAAKT----PIQWEERNVtaikgpGGRW------MIPPEAKESMDKNKIGLKGPLKTP 101
Cdd:COG0473   3 KIAVLPGDGIGPEVVAAALKVLEAAAErfglDFEFEEADI------GGAAydktgtPLPDETLEALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 102 IAAGHP---SMNLLLRKTFDLYANVRPCVSIEGYKTPY-----TDVDLVTIRENTEGEYSGIEHVIVDG----VVQSIKL 169
Cdd:COG0473  77 KWDDGVrpeSGLLALRKELDLYANLRPAKLYPGLPSPLkpeivEGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRV 156

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 170 ITEEASRRIAEYAFEYARNNqRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLV 249
Cdd:COG0473 157 YTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIV 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 250 MPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETA 329
Cdd:COG0473 236 TENLFGDILSDLAAGLTGSLGLAPSANIGDEGKALFEPVHGSAPDIAGKGIANPIATILSAAMMLRHLGEEEAADAIEAA 315

                       330       340       350
                ....*....|....*....|....*....|..
gi 46358344 330 CFDTIRDkKVLTKDLGGNSKCSEFTADICRRV 361
Cdd:COG0473 316 VEKVLAE-GVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
32-357 2.78e-151

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 429.79  E-value: 2.78e-151
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    32 TVTLIPGDGIGPEISTAVMKIFEAAK----TPIQWEERNVTAIKGPGGRWMIPPEAKESMDKNKIGLKGPLKTPIA--AG 105
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALekapLEFEFEERDVGGAAIDETGEPLPDETLEACKKADAVLLGAVGGPKWdpAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   106 HPSMNLL--LRKTFDLYANVRPCVSI--EGYKTPY----TDVDLVTIRENTEGEYSGIEHVIVDG---VVQSIKLITEEA 174
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFppLGDASPLknevEGVDIVIVRELTGGIYFGIEKGIKGSgneVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   175 SRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLY 254
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   255 GDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRH-MGLHGHAKKIETACFDT 333
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGANGFGIFEPVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKV 320

                         330       340
                  ....*....|....*....|....*..
gi 46358344   334 IRDKKvLTKDLGGNS---KCSEFTADI 357
Cdd:pfam00180 321 LESGI-RTGDLAGSAtyvSTSEFGEAV 346
AksF2_Meth NF040626
homoisocitrate dehydrogenase;
32-357 9.02e-109

homoisocitrate dehydrogenase;


Pssm-ID: 468598  Cd Length: 332  Bit Score: 321.30  E-value: 9.02e-109
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   32 TVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEErnvtaikGPGGR-------WMIPPEAKESMDKNKIGLKGPLKTpiAA 104
Cdd:NF040626   3 KITVIPGDGIGKEVMEAALYVLDALDLNFDFIE-------AEAGRecfkkngTTIPEETIKIAKKSDATLFGAVTS--TP 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  105 GHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFE 184
Cdd:NF040626  74 GQKSPIITLRKELDLYANLRPIKSYEGINCLFKDLDFLIVRENTEGLYSGLEEEYTEEKAIAERVITRKASERICKFAFE 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  185 YARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAG 264
Cdd:NF040626 154 YAIKLGRKKVTAVHKANVLKKTDGIFKDTFYKVAKDYPQIETEDYYVDATAMYLITKPQDFDVIVTTNLFGDILSDEAAG 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  265 LIGGLGVTPSGNIGANGvAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDL 344
Cdd:NF040626 234 LVGGLGLAPSANIGDKN-GLFEPVHGSAPDIAGKNIANPIAMILSASMMLDYLGETYEANKLENALEKVLREGKVVTPDL 312

                        330
                 ....*....|...
gi 46358344  345 GGNSKCSEFTADI 357
Cdd:NF040626 313 GGNAKTMEMANEI 325
AksF_Meth NF040619
homoisocitrate dehydrogenase;
33-357 1.58e-80

homoisocitrate dehydrogenase;


Pssm-ID: 468591  Cd Length: 332  Bit Score: 249.29  E-value: 1.58e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIF-EAAKTPIQWEERNVTAIKGPGGrwMIPPEAKESMDKNKIGLKGPLKTPIAAGHPSMN- 110
Cdd:NF040619   4 ICVIEGDGIGKEVIPETVRVLkELGDFEFIKGEAGLECFKKYGD--AIPEETIEKAKECDAILFGAVTTPKPTELKNKNy 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  111 ----LLLRKTFDLYANVRPcVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDG-VVQSIKLITEEASRRIAEYAFEY 185
Cdd:NF040619  82 kspiLTLRKELDLYANVRP-INNFGDGQDVKNIDFVIIRENTEGLYVGREYYDEENeIAIAERIISKKGSERIIKFAFEY 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  186 ARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKD--VKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCA 263
Cdd:NF040619 161 AKKNNRKKVSCIHKANVLRVTDGLFLEIFNEIKKKYKNfnIEADDYLVDATAMYLIKNPEMFDVIVTTNLFGDILSDEAS 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  264 GLIGGLGVTPSGNIGANgVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKD 343
Cdd:NF040619 241 GLIGGLGLAPSANIGDK-KGLFEPVHGSAPDIAGKGIANPIATILSAAMMLDYLGMKEKGDLIREAVKKCLENGKV-TPD 318

                        330
                 ....*....|....
gi 46358344  344 LGGNSKCSEFTADI 357
Cdd:NF040619 319 LGGNLKTKEVTDKI 332
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 29-361 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 557.77  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    29 GIQTVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGpgGRWMIPPEAKESMDKNKIGLKGPLKTPIAAG-HP 107
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTD--GKTEIPDEAVESIKRNKVALKGPLETPIGKGgHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   108 SMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYAR 187
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   188 NNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIG 267
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   268 GLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDLGGN 347
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 46358344   348 SKCSEFTADICRRV 361
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 7-358 6.93e-170

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 478.22  E-value: 6.93e-170
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    7 SAVSCIMGAIKTQTPQPRVFSRGIQ------TVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGPGGRWMIP 80
Cdd:PLN00118  12 NRLAQILGASSSSSGAFSSSARAFSssstpiTATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTGSFLT 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   81 PEAKESMDKNKIGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIV 160
Cdd:PLN00118  92 WESLESVRRNKVGLKGPMATPIGKGHRSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVV 171

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  161 DGVVQSIKLITEEASRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQ 240
Cdd:PLN00118 172 RGVVESLKIITRQASLRVAEYAFHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVK 251

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  241 DPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLH 320
Cdd:PLN00118 252 NPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLKLN 331

                        330       340       350
                 ....*....|....*....|....*....|....*...
gi 46358344  321 GHAKKIETACFDTIRDKKVLTKDLGGNSKCSEFTADIC 358
Cdd:PLN00118 332 EQAEQIHNAILNTIAEGKYRTADLGGSSTTTDFTKAIC 369
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 32-361 4.41e-153

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 434.44  E-value: 4.41e-153
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  32 TVTLIPGDGIGPEISTAVMKIFEAAKT----PIQWEERNVtaikgpGGRW------MIPPEAKESMDKNKIGLKGPLKTP 101
Cdd:COG0473   3 KIAVLPGDGIGPEVVAAALKVLEAAAErfglDFEFEEADI------GGAAydktgtPLPDETLEALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 102 IAAGHP---SMNLLLRKTFDLYANVRPCVSIEGYKTPY-----TDVDLVTIRENTEGEYSGIEHVIVDG----VVQSIKL 169
Cdd:COG0473  77 KWDDGVrpeSGLLALRKELDLYANLRPAKLYPGLPSPLkpeivEGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRV 156

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 170 ITEEASRRIAEYAFEYARNNqRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLV 249
Cdd:COG0473 157 YTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIV 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 250 MPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETA 329
Cdd:COG0473 236 TENLFGDILSDLAAGLTGSLGLAPSANIGDEGKALFEPVHGSAPDIAGKGIANPIATILSAAMMLRHLGEEEAADAIEAA 315

                       330       340       350
                ....*....|....*....|....*....|..
gi 46358344 330 CFDTIRDkKVLTKDLGGNSKCSEFTADICRRV 361
Cdd:COG0473 316 VEKVLAE-GVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
32-357 2.78e-151

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 429.79  E-value: 2.78e-151
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    32 TVTLIPGDGIGPEISTAVMKIFEAAK----TPIQWEERNVTAIKGPGGRWMIPPEAKESMDKNKIGLKGPLKTPIA--AG 105
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALekapLEFEFEERDVGGAAIDETGEPLPDETLEACKKADAVLLGAVGGPKWdpAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   106 HPSMNLL--LRKTFDLYANVRPCVSI--EGYKTPY----TDVDLVTIRENTEGEYSGIEHVIVDG---VVQSIKLITEEA 174
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFppLGDASPLknevEGVDIVIVRELTGGIYFGIEKGIKGSgneVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   175 SRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLY 254
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   255 GDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRH-MGLHGHAKKIETACFDT 333
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGANGFGIFEPVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKV 320

                         330       340
                  ....*....|....*....|....*..
gi 46358344   334 IRDKKvLTKDLGGNS---KCSEFTADI 357
Cdd:pfam00180 321 LESGI-RTGDLAGSAtyvSTSEFGEAV 346
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 31-361 5.79e-130

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 375.60  E-value: 5.79e-130
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   31 QTVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGPGGRWMIPPEAKESMDKNKIGLKGPLKTPIAAGHPSMN 110
Cdd:PRK08997   3 QTITVIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGELLPQRTLDLIEKNKIALKGPLTTPVGEGFTSIN 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  111 LLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGI-EHVIVDG-VVQSIKLITEEASRRIAEYAFEYARN 188
Cdd:PRK08997  83 VTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEgQTVSEDGeTAEATSIITRKGAERIVRFAYELARK 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  189 NQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGG 268
Cdd:PRK08997 163 EGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVGG 242

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  269 LGVTPSGNIGANGvAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDLGGNS 348
Cdd:PRK08997 243 LGMAPGANIGRDA-AIFEAVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDLGGTH 321

                        330
                 ....*....|...
gi 46358344  349 KCSEFTADICRRV 361
Cdd:PRK08997 322 GTTDFTQAVIDRL 334
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
33-344 1.50e-127

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 374.61  E-value: 1.50e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTA---IKG-PGGrwmIPPEAKESMDKNKIGLKGPLKTPIAAGHPS 108
Cdd:PRK09222   7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEkvyKKGwTSG---ISPSAWESIRRTKVLLKAPITTPQGGGYKS 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  109 MNLLLRKTFDLYANVRPCVSIEGY-KTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYAR 187
Cdd:PRK09222  84 LNVTLRKTLGLYANVRPCVSYHPFvETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEYAR 163

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  188 NNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIG 267
Cdd:PRK09222 164 ANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEISG 243

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 46358344  268 GLGVTPSGNIGaNGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDkKVLTKDL 344
Cdd:PRK09222 244 SVGLAGSANIG-EEYAMFEAVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLED-GIHTADI 318
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 17-362 3.10e-127

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 369.58  E-value: 3.10e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   17 KTQTPQPRVFSRGIQTVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTaikgpGGRWMIPPEAKESMDKNKIGLKG 96
Cdd:PLN00123  17 RSVTYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVH-----GDMKKVPEEVLESIRRNKVCLKG 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   97 PLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASR 176
Cdd:PLN00123  92 GLATPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSE 171

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  177 RIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGD 256
Cdd:PLN00123 172 RIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGN 251

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  257 ILSDLCAGLIGGLGVTPSGNIGAnGVAIFESVHGTA----PDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFD 332
Cdd:PLN00123 252 LVANTAAGIAGGTGVMPGGNVGA-DHAVFEQGASAGnvgnEKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKR 330

                        330       340       350
                 ....*....|....*....|....*....|
gi 46358344  333 TIRDKKVLTKDLGGNSKCSEFTADICRRVQ 362
Cdd:PLN00123 331 VIAEGKYRTKDLGGSSTTQEVVDAVIANLD 360
AksF2_Meth NF040626
homoisocitrate dehydrogenase;
32-357 9.02e-109

homoisocitrate dehydrogenase;


Pssm-ID: 468598  Cd Length: 332  Bit Score: 321.30  E-value: 9.02e-109
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   32 TVTLIPGDGIGPEISTAVMKIFEAAKTPIQWEErnvtaikGPGGR-------WMIPPEAKESMDKNKIGLKGPLKTpiAA 104
Cdd:NF040626   3 KITVIPGDGIGKEVMEAALYVLDALDLNFDFIE-------AEAGRecfkkngTTIPEETIKIAKKSDATLFGAVTS--TP 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  105 GHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFE 184
Cdd:NF040626  74 GQKSPIITLRKELDLYANLRPIKSYEGINCLFKDLDFLIVRENTEGLYSGLEEEYTEEKAIAERVITRKASERICKFAFE 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  185 YARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAG 264
Cdd:NF040626 154 YAIKLGRKKVTAVHKANVLKKTDGIFKDTFYKVAKDYPQIETEDYYVDATAMYLITKPQDFDVIVTTNLFGDILSDEAAG 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  265 LIGGLGVTPSGNIGANGvAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVLTKDL 344
Cdd:NF040626 234 LVGGLGLAPSANIGDKN-GLFEPVHGSAPDIAGKNIANPIAMILSASMMLDYLGETYEANKLENALEKVLREGKVVTPDL 312

                        330
                 ....*....|...
gi 46358344  345 GGNSKCSEFTADI 357
Cdd:NF040626 313 GGNAKTMEMANEI 325
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 33-361 4.17e-106

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 314.76  E-value: 4.17e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIFEAAKTPIQ--WEERNVTAIKGPGGrwMIPPEAKESMDKNKIGLKGplktpiAAGHPSMN 110
Cdd:PRK14025   4 ICVIEGDGIGKEVVPAALHVLEATGLPFEfvYAEAGDEVFEKTGK--ALPEETIEAAKEADAVLFG------AAGETAAD 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  111 LL--LRKTFDLYANVRPCVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYARN 188
Cdd:PRK14025  76 VIvkLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEMAKR 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  189 NQ----RTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAG 264
Cdd:PRK14025 156 RKkmgkEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDGAAG 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  265 LIGGLGVTPSGNIGaNGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKDL 344
Cdd:PRK14025 236 LVGGLGLAPSANIG-DKYGLFEPVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLALGLT-TPDL 313

                        330
                 ....*....|....*..
gi 46358344  345 GGNSKCSEFTADICRRV 361
Cdd:PRK14025 314 GGNLSTMEMAEEVAKRV 330
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 33-357 5.35e-106

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 314.01  E-value: 5.35e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    33 VTLIPGDGIGPEISTAVMKIFEAAKTPIQWeernvtaIKGPGG--RW-----MIPPEAKESMDKNKIGLKGPLKTPIAAG 105
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEF-------IEFEAGdeALkkygsALPEDTLEEIRKADAILFGAVTTPANPG 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   106 HPSMNLLLRKTFDLYANVRPCVSIEGYKTPY-TDVDLVTIRENTEGEYSGIEHVIVDGVVqSIKLITEEASRRIAEYAFE 184
Cdd:TIGR02088  74 YKSVIVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFEFGFSDRAI-AIRVITREGSERIARFAFN 152

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   185 YARNNQRtSVTAVHKANIMRMSDGLFLRKCREVAENfKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAG 264
Cdd:TIGR02088 153 LAKERNR-KVTCVHKANVLKGTDGLFREVCREIAKR-YGVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASA 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   265 LIGGLGVTPSGNIGaNGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKDL 344
Cdd:TIGR02088 231 LAGSLGLAPSANIG-DRKALFEPVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDL 308

                         330
                  ....*....|...
gi 46358344   345 GGNSKCSEFTADI 357
Cdd:TIGR02088 309 GGTAKTKEVGDEI 321
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 33-343 9.81e-97

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 295.52  E-value: 9.81e-97
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    33 VTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNV-TAIKGPGGRWMIPPEAKESMDKNKIGLKGPLKTPIAAGHPSMNL 111
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEIgEKVYKKGWPSGISPSSWESIRRTKVLLKAPITTPQGGGHKSLNV 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   112 LLRKTFDLYANVRPCVSIEGY-KTPYTDVDLVTIRENTEGEYSGIEHVIVDGVVQSIKLITEEASRRIAEYAFEYARNNQ 190
Cdd:TIGR02924  83 TLRKTLGLYANIRPCVSYHPFiETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARKHN 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   191 RTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLG 270
Cdd:TIGR02924 163 RKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGSVG 242

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   271 VTPSGNIGANGvAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRD-------------- 336
Cdd:TIGR02924 243 LAGSANIGEEY-AMFEAVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLEDgvhtadiynektsk 321


                  ....*..
gi 46358344   337 KKVLTKD 343
Cdd:TIGR02924 322 QKVGTKE 328
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 36-363 2.13e-90

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 276.99  E-value: 2.13e-90
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  36 IPGDGIGPEISTAVMKIFEAA-------KTPIQWEE-------RNVTaikgpgGRWmIPPEAKESMDKNKIGLKGPLKTP 101
Cdd:COG0538  24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEvdagekaRDET------GDW-LPDETAEAIKEYGVGIKGPLTTP 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 102 IAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTP---YTDVDLVTIRENTEGEYSGIE--------------------HV 158
Cdd:COG0538  97 VGGGWRSLNVTIRQILDLYVCRRPVRYFKGVPSPvkhPEKVDIVIFRENTEDIYAGIEwkagspealkliffledemgVT 176

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 159 IVD-----GVVqsIKLITEEASRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAEN-FKDVKFTE---- 228
Cdd:COG0538 177 VIRfpedsGIG--IKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEEeFGDKFITEgpwe 254

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344 229 -----------MYLDTVCLNMVQD----PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAP 293
Cdd:COG0538 255 kykgpkpagkiVYKDRIADDMLQQillrPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGDDGGAEFEATHGTAP 334

                       330       340       350       360       370       380       390
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 46358344 294 DIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKDL-----GGNS-KCSEFTADICRRVQD 363
Cdd:COG0538 335 KYAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDLarlmeGATElSTSEFGDAIIENLDK 409
AksF_Meth NF040619
homoisocitrate dehydrogenase;
33-357 1.58e-80

homoisocitrate dehydrogenase;


Pssm-ID: 468591  Cd Length: 332  Bit Score: 249.29  E-value: 1.58e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIF-EAAKTPIQWEERNVTAIKGPGGrwMIPPEAKESMDKNKIGLKGPLKTPIAAGHPSMN- 110
Cdd:NF040619   4 ICVIEGDGIGKEVIPETVRVLkELGDFEFIKGEAGLECFKKYGD--AIPEETIEKAKECDAILFGAVTTPKPTELKNKNy 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  111 ----LLLRKTFDLYANVRPcVSIEGYKTPYTDVDLVTIRENTEGEYSGIEHVIVDG-VVQSIKLITEEASRRIAEYAFEY 185
Cdd:NF040619  82 kspiLTLRKELDLYANVRP-INNFGDGQDVKNIDFVIIRENTEGLYVGREYYDEENeIAIAERIISKKGSERIIKFAFEY 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  186 ARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKD--VKFTEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCA 263
Cdd:NF040619 161 AKKNNRKKVSCIHKANVLRVTDGLFLEIFNEIKKKYKNfnIEADDYLVDATAMYLIKNPEMFDVIVTTNLFGDILSDEAS 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  264 GLIGGLGVTPSGNIGANgVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKD 343
Cdd:NF040619 241 GLIGGLGLAPSANIGDK-KGLFEPVHGSAPDIAGKGIANPIATILSAAMMLDYLGMKEKGDLIREAVKKCLENGKV-TPD 318

                        330
                 ....*....|....
gi 46358344  344 LGGNSKCSEFTADI 357
Cdd:NF040619 319 LGGNLKTKEVTDKI 332
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 33-363 2.57e-77

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 241.93  E-value: 2.57e-77
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIFEAAKT----PIQWEERNV--TAIKGPGGrwMIPPE----AKESmDKNKIGLKGPLK--T 100
Cdd:PRK00772   5 IAVLPGDGIGPEVMAEAVKVLDAVAEkfgfDFEFEEALVggAAIDAHGV--PLPEEtleaCRAA-DAVLLGAVGGPKwdN 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  101 PIAAGHPSMNLL-LRKTFDLYANVRPCVSIEGYKTPYT-------DVDLVTIRENTEGEYSG----IEHVIVDGVVQSIK 168
Cdd:PRK00772  82 LPPDVRPERGLLaLRKELGLFANLRPAKLYPGLADASPlkpeivaGLDILIVRELTGGIYFGeprgREGLGGEERAFDTM 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  169 LITEEASRRIAEYAFEYARNnQRTSVTAVHKANIMRMSdgLFLRK-CREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDV 247
Cdd:PRK00772 162 VYTREEIERIARVAFELARK-RRKKVTSVDKANVLESS--RLWREvVTEVAKEYPDVELSHMYVDNAAMQLVRNPKQFDV 238

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  248 LVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRH-MGLHGHAKKI 326
Cdd:PRK00772 239 IVTENLFGDILSDEAAMLTGSLGMLPSASLGESGPGLYEPIHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEEAADAI 318

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|
gi 46358344  327 ETACFDTIrDKKVLTKDL---GGNSKCSEFTADICRRVQD 363
Cdd:PRK00772 319 EAAVEKVL-AQGYRTADIaegGGKVSTSEMGDAILAALAE 357
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 36-361 5.79e-71

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 225.19  E-value: 5.79e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   36 IPGDGIGPEISTAVMKIFEAAKTPIQWEERnvTAIKGPGGRWM-----IPPEAKESMDKNKIGLKGplktpiAAGHPSM- 109
Cdd:PRK03437  10 IPGDGIGPEVVAEALKVLDAVAAGGPGVET--TEYDLGARRYLrtgetLPDSVLAELRQHDAILLG------AIGDPSVp 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  110 ------NLLLRKTFDL--YANVRPCVSIEGYKTPYT---DVDLVTIRENTEGEYSGIEHVIVDG----VVQSIKLITEEA 174
Cdd:PRK03437  82 sgvlerGLLLKLRFALdhYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGNGGALRVGtpheVATEVSVNTAFG 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  175 SRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLVMPNLY 254
Cdd:PRK03437 162 VERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDVIVTDNLF 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  255 GDILSDLCAGLIGGLGVTPSGNIGANGVA--IFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAKKIETACfd 332
Cdd:PRK03437 242 GDIITDLAAAVTGGIGLAASGNINPTGTNpsMFEPVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAARIEAAV-- 319

                        330       340
                 ....*....|....*....|....*....
gi 46358344  333 tirdKKVLTKDLGGNSKCSEFTADICRRV 361
Cdd:PRK03437 320 ----EADLAERGKMGRSTAEVGDRIAARL 344
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 36-353 2.43e-70

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 225.71  E-value: 2.43e-70
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   36 IPGDGIGPEISTAVMKIFEAA-------KTPIQWEE----RNVTAIKGPGgRWMiPPEAKESMDKNKIGLKGPLKTPIAA 104
Cdd:PRK07006  25 IEGDGIGPDITPAMLKVVDAAvekaykgERKISWMEiyagEKATKVYGED-VWL-PEETLDLIREYRVAIKGPLTTPVGG 102

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  105 GHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYT---DVDLVTIRENTEGEYSGIEHVI----VDGVVQ------------ 165
Cdd:PRK07006 103 GIRSLNVALRQELDLYVCLRPVRYFKGVPSPVKrpeDTDMVIFRENSEDIYAGIEWKAgsaeAKKVIKflqeemgvkkir 182

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  166 -------SIKLITEEASRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAEN-FKDVKFT---------- 227
Cdd:PRK07006 183 fpetsgiGIKPVSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKDWGYQLAEEeFGDELIDggpwdkiknp 262

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  228 EMYLDTVCLNMVQD---------PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGaNGVAIFESVHGTAPDIAGK 298
Cdd:PRK07006 263 ETGKEIIVKDSIADaflqqillrPAEYDVIATMNLNGDYISDALAAQVGGIGIAPGANIN-DGHAIFEATHGTAPKYAGL 341

                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 46358344  299 DMANPTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKDL-----GGNS-KCSEF 353
Cdd:PRK07006 342 DKVNPGSVILSAEMMLRHMGWTEAADLIIKSMEKTIASKTV-TYDFarlmeGATEvKCSEF 401
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
36-344 1.00e-69

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 223.94  E-value: 1.00e-69
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   36 IPGDGIGPEISTAVMKIFEAA-------KTPIQWEE--RNVTAIKGPGGRWmiPPEAKESMDKNKIGLKGPLKTPIAAGH 106
Cdd:PRK06451  29 VEGDGIGPEITHAAMKVINKAvekaygsDREIKWVEvlAGDKAEKLTGNRF--PKESEELIEKYRVLLKGPLETPIGKGW 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  107 PSMNLLLRKTFDLYANVRPCVSIEGYKTPYTD---VDLVTIRENTEGEYSGIEHV--------------------IVDGV 163
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYPydseeakkirdflrkelgveVEDDT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  164 VQSIKLITEEASRRIAEYAFEYARNNQRTSVTAVHKANIMRMSDGLFLRKCREVAEN-FKDVKFTE-------------- 228
Cdd:PRK06451 187 GIGIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVALKeFRDYVVTEeevtknyngvppsg 266

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  229 --MYLDTVCLNMVQD----PSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGvAIFESVHGTAPDIAGKDMAN 302
Cdd:PRK06451 267 kvIINDRIADNMFQQiiirPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTG-GMFEAIHGTAPKYAGKNVAN 345

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|..
gi 46358344  303 PTALLLSAVMMLRHMGLHGHAKKIETACFDTIRDKKVlTKDL 344
Cdd:PRK06451 346 PTGIIKGGELMLRFMGWDKAADLIDKAIMESIKQKKV-TQDL 386
TTC TIGR02089
tartrate dehydrogenase; Tartrate dehydrogenase catalyzes the oxidation of both meso- and (+) ...
 33-361 2.15e-68

tartrate dehydrogenase; Tartrate dehydrogenase catalyzes the oxidation of both meso- and (+)-tartrate as well as a D-malate. These enzymes are closely related to the 3-isopropylmalate and isohomocitrate dehydrogenases found in TIGR00169 and TIGR02088, respectively. [Energy metabolism, Other]


Pssm-ID: 273963  Cd Length: 352  Bit Score: 218.94  E-value: 2.15e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    33 VTLIPGDGIGPEISTAVMKIFEAAKtpiqwEERNVTAIKGPGGRW----------MIPPEAKESMDKNK---IGLKG-PL 98
Cdd:TIGR02089   6 IAAIPGDGIGKEVVAAALQVLEAAA-----KRHGGFSLHFTEFPWscdyykehgkMMPEDGLEKLKKFDaifLGAVGwPA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    99 KTPiaaGHPSMNLLL---RKTFDLYANVRPCVSIEGYKTPY-----TDVDLVTIRENTEGEYSGIEHVIVDG----VVQS 166
Cdd:TIGR02089  81 LVP---DHISLWGLLlkiRREFDQYANVRPAKLLPGVTSPLrncgpGDFDFVVVRENSEGEYSGVGGRIHRGtdeeVATQ 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   167 IKLITEEASRRIAEYAFEYARNnQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFD 246
Cdd:TIGR02089 158 NAIFTRKGVERIMRFAFELAQK-RRKHLTSATKSNGIRHSMPFWDEVFAEVAAEYPDVEWDSYHIDALAARFVLKPETFD 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   247 VLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVA--IFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAK 324
Cdd:TIGR02089 237 VIVASNLFGDILSDLGAALMGSLGVAPSANINPEGKFpsMFEPVHGSAPDIAGKGIANPIGAIWTAAMMLEHLGEKEAGA 316

                         330       340       350
                  ....*....|....*....|....*....|....*..
gi 46358344   325 KIETAcFDTIRDKKVLTKDLGGNSKCSEFTADICRRV 361
Cdd:TIGR02089 317 KIMDA-IERVTAAGILTPDVGGKATTSEVTEAVCNAL 352
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 33-361 1.41e-64

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 208.79  E-value: 1.41e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344    33 VTLIPGDGIGPEISTAVMKIFEA-AKTP---IQWEERNV--TAIKGPGGRwmIPPEAKESMDKNKIGLKGPLKTP----- 101
Cdd:TIGR00169   2 IAVLPGDGIGPEVMAQALKVLKAvAERFglkFEFEEHLIggAAIDATGQP--LPEETLKGCKEADAVLLGAVGGPkwdnl 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   102 IAAGHPSMNLL-LRKTFDLYANVRPCVSIEGY------KTPYTD-VDLVTIRENTEGEY----SGIEhviVDGVVQSIKL 169
Cdd:TIGR00169  80 PRDQRPEQGLLkLRKSLDLFANLRPAKVFPGLedlsplKEEIAKgVDFVVVRELTGGIYfgepKGRE---GEGEAWDTEV 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   170 ITEEASRRIAEYAFEYARNnQRTSVTAVHKANIMRMSDgLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLV 249
Cdd:TIGR00169 157 YTVPEIERIARVAFEMARK-RRKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVV 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   250 MPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRH-MGLHGHAKKIET 328
Cdd:TIGR00169 235 TSNLFGDILSDEASVITGSLGMLPSASLGSDGFGLFEPVHGSAPDIAGKGIANPIAQILSAAMLLRYsFNLEEAADAIEA 314

                         330       340       350
                  ....*....|....*....|....*....|....*...
gi 46358344   329 ACfdtirdKKVL-----TKDLGGNSKCSEFTADICRRV 361
Cdd:TIGR00169 315 AV------KKVLaegyrTPDLGSSATTAVGTAEMGEEL 346
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 32-362 2.17e-61

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 200.72  E-value: 2.17e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   32 TVTLIPGDGIGPEISTAVMKIFEAA----------KTPIQWEernvTAIKGPGGRwMIPPEAKESMDKNKIGLKGPLKTP 101
Cdd:PRK08194   5 KIAVIPGDGVGKEVVPAAVRVLKAVaevhgglkfeFTEFPWS----CEYYLEHGE-MMPEDGLEQLKQFDAIFLGAVGNP 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  102 -IAAGHPSM-NLLL--RKTFDLYANVRPCVSIEGYKTPYT---DVDLVTIRENTEGEYSGIEHVIVDG----VVQSiKLI 170
Cdd:PRK08194  80 kLVPDHISLwGLLIkiRREFEQVINIRPAKQLRGIKSPLAnpkDFDLLVVRENSEGEYSEVGGRIHRGedeiAIQN-AVF 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  171 TEEASRRIAEYAFEYARNnQRTSVTAVHKAN----IMRMSDGLFlrkcREVAENFKDVKFTEMYLDTVCLNMVQDPSQFD 246
Cdd:PRK08194 159 TRKGTERAMRYAFELAAK-RRKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEEFD 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  247 VLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGV--AIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGHAK 324
Cdd:PRK08194 234 VIVASNLFGDILTDIGAAIMGSIGIAPAANINVNGKypSMFEPVHGSAPDIAGKGIANPIGQIWTAKLMLDHFGEEELGS 313

                        330       340       350
                 ....*....|....*....|....*....|....*...
gi 46358344  325 KIETACFDTIRDkKVLTKDLGGNSKCSEFTADICRRVQ 362
Cdd:PRK08194 314 HLLDVIEDVTED-GIKTPDIGGRATTDEVTDEIISRLK 350
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
33-360 4.63e-47

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 166.43  E-value: 4.63e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIFEAAKTPIQWEERNVTAIKGPGGR---------WMIPPEAKESMDKNKIGLKGPLKTPIA 103
Cdd:PRK07362  31 IPFIRGDGTGVDIWPATQKVLDAAVAKAYGGERKINWFKVYAGDeacdlygtyQYLPEDTLEAIREYGVAIKGPLTTPIG 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  104 AGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYT---DVDLVTIRENTEGEYSGIE------------------------ 156
Cdd:PRK07362 111 GGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGIEweagdeigdklikhlneevipasp 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  157 ----HVIVDGVVQSIKLITEEASRRIAEYAFEYAR--NNQRTSVTAVHKANIMRMSDGLFLRKCREVAEN-FKDVKFTEM 229
Cdd:PRK07362 191 elgkRQIPLGSGIGIKPVSKTGSQRHIRRAIEHALrlPGDKRHVTLVHKGNIMKYTEGAFRDWGYELATTeFRDECVTER 270

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  230 ---YLDTVCLN---------------------------------------------------MVQD-------------P 242
Cdd:PRK07362 271 eswILSNKEKNpnisiednarmiepgydsltpekkaaicaevkevldsiwsshgngkwkekvLVDDriadsifqqiqtrP 350

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  243 SQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANgVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLHGH 322
Cdd:PRK07362 351 QEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDN-AAIFEATHGTAPKHAGLDRINPGSVILSGVMMLEYLGWQEA 429

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|....*
gi 46358344  323 AKKIETACFDTIRDKKVlTKDLG-------GNSKCSEFTADICRR 360
Cdd:PRK07362 430 ADLITKGLSAAIANKQV-TYDLArlmeppvDPLSCSEFAEAIISH 473
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 33-362 2.24e-43

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 155.23  E-value: 2.24e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344   33 VTLIPGDGIGPEISTAVMKIFEAAKTpIQWEERNVTAIKGPGGRW-----MIPPE---AKESMDKNKIGLKGPLKTPIAA 104
Cdd:PLN02329  49 IALLPGDGIGPEVISVAKNVLQKAGS-LEGLEFDFQEMPVGGAALdlvgvPLPEEtftAAKQSDAILLGAIGGYKWDKNE 127

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  105 GH--PSMNLL-LRKTFDLYANVRPCVSIEGY-------KTPYTDVDLVTIRENTEGEYSGIEHVIV-----DGVVQSIKL 169
Cdd:PLN02329 128 KHlrPEMALFyLRRDLKVFANLRPATVLPQLvdastlkKEVAEGVDMMIVRELTGGIYFGEPRGITinengEEVGVSTEI 207

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  170 ITEEASRRIAEYAFEYARNnQRTSVTAVHKANIMRMSDgLFLRKCREVAENFKDVKFTEMYLDTVCLNMVQDPSQFDVLV 249
Cdd:PLN02329 208 YAAHEIDRIARVAFETARK-RRGKLCSVDKANVLDASI-LWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQFDTIV 285

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  250 MPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRH-MGLHGHAKKIET 328
Cdd:PLN02329 286 TNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEEKAAKRIED 365

                        330       340       350
                 ....*....|....*....|....*....|....*...
gi 46358344  329 ACFDTIrDKKVLTKDL--GGNS--KCSEFTADICRRVQ 362
Cdd:PLN02329 366 AVVDAL-NKGFRTGDIysPGNKlvGCKEMGEEVLKSVD 402
PLN03065 PLN03065
isocitrate dehydrogenase (NADP+); Provisional
 171-344 1.48e-05

isocitrate dehydrogenase (NADP+); Provisional


Pssm-ID: 178617 [Multi-domain]  Cd Length: 483  Bit Score: 46.80  E-value: 1.48e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  171 TEEASRRIAEYAFEYARNnQRTSVTAVHKANIMRMSDGLFLRKCREVAENFKDVKFTEM---YLDTVCLNMVQDPSQFD- 246
Cdd:PLN03065 254 VDESIRAFAESSMAMALQ-KKWPLYLSTKNTILKKYDGRFKDIFQEVYEEQWKQKFEEHsiwYEHRLIDDMVAYAVKSEg 332

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 46358344  247 --VLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAI-FESVHGTAPD-----IAGKDMA-NPTALLLSAVMMLRHM 317
Cdd:PLN03065 333 gyVWACKNYDGDVQSDLLAQGFGSLGLMTSVLLSSDGKTLeAEAAHGTVTRhfrlhQKGQETStNSIASIFAWTRGLEHR 412

                        170       180       190
                 ....*....|....*....|....*....|....
gi 46358344  318 G-------LHGHAKKIETACFDTIRDKKvLTKDL 344
Cdd:PLN03065 413 AkldkneeLLDFVHKLESACIETVESGK-MTKDL 445
PdxA COG1995
4-hydroxy-L-threonine phosphate dehydrogenase PdxA [Coenzyme transport and metabolism]; ...
 287-316 2.91e-03

4-hydroxy-L-threonine phosphate dehydrogenase PdxA [Coenzyme transport and metabolism]; 4-hydroxy-L-threonine phosphate dehydrogenase PdxA is part of the Pathway/BioSystem: Pyridoxal phosphate biosynthesis


Pssm-ID: 441598  Cd Length: 330  Bit Score: 39.28  E-value: 2.91e-03
                        10        20        30
                ....*....|....*....|....*....|....
gi 46358344 287 SV-HGTAPDIAGKDMANPTAL---LLSAVMMLRH 316
Cdd:COG1995 295 SVdHGTAFDIAGKGIADPGSLiaaIRLAARLARN 328
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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