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Conserved domains on  [gi|31983701|ref|NP_858345|]
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ISSfl4 ORF2 (plasmid) [Shigella flexneri 2a str. 301]

Protein Classification

IS66 family insertion sequence element accessory protein TnpB( domain architecture ID 16678254)

IS66 family insertion sequence element accessory protein TnpB is part of a transposase complex that binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803|GO:0003677|GO:0006313|GO:0032196
PubMed:  30454069|11418571|20215432|11418571|20615441|17165262|20079432

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 10-99 7.69e-52

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


:

Pssm-ID: 411392  Cd Length: 90  Bit Score: 158.03  E-value: 7.69e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701   10 IWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLT 89
Cdd:NF033819   1 IYLATGPTDMRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALT 80

                         90
                 ....*....|
gi 31983701   90 PAQLSMLLEG 99
Cdd:NF033819  81 PAQLAWLLEG 90
 
Name Accession Description Interval E-value
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 10-99 7.69e-52

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 158.03  E-value: 7.69e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701   10 IWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLT 89
Cdd:NF033819   1 IYLATGPTDMRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALT 80

                         90
                 ....*....|
gi 31983701   90 PAQLSMLLEG 99
Cdd:NF033819  81 PAQLAWLLEG 90
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 13-107 3.47e-50

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 154.17  E-value: 3.47e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701    13 IAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPV-TREGKVHLTPA 91
Cdd:pfam05717   1 ATGPTDMRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAA 80

                          90
                  ....*....|....*.
gi 31983701    92 QLSMLLEGIAWPHPKR 107
Cdd:pfam05717  81 QLSWLLEGIDWRRPRA 96
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
12-102 6.78e-07

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 46.18  E-value: 6.78e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701  12 IIAGITDMRCgFNGLA---SKVQNTLKDDPfsGHIFVFRGRSGKmVKILWADRDGLC---LFAKRLERG----------- 74
Cdd:COG3436  79 VLHEPTDMRK-IDGLAaigEEVSEELDLDP--GALFVFRNRRDK-YKCLYCDGTGLVyapAPAKRLEKGlagpgllahil 154

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 31983701  75 --RFV--WPVTREGK------VHLTPAQLSMLLEGIAW 102
Cdd:COG3436 155 vsKFVdhLPLYRQEEifarlgVELSRSTLLNWVGRAAE 192
 
Name Accession Description Interval E-value
IS66_TnpB NF033819
IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion ...
 10-99 7.69e-52

IS66 family insertion sequence element accessory protein TnpB; The IS66 family insertion sequence element encodes a DDE transposase TnpC, and two accessory proteins, TnpA and TnpB. It has been assumed that the TnpA, TnpB, and TnpC proteins are produced independently in appropriate amounts and form a complex, which acts as a transposase to promote the transposition of an IS66 family element.


Pssm-ID: 411392  Cd Length: 90  Bit Score: 158.03  E-value: 7.69e-52
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701   10 IWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLT 89
Cdd:NF033819   1 IYLATGPTDMRKGIDGLAALVQEVLGLDPFSGHLFVFRNRRRDRLKILYWDGSGFCLYYKRLEKGRFRWPRAADGVVALT 80

                         90
                 ....*....|
gi 31983701   90 PAQLSMLLEG 99
Cdd:NF033819  81 PAQLAWLLEG 90
TnpB_IS66 pfam05717
IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The ...
 13-107 3.47e-50

IS66 Orf2 like protein; This protein is found in insertion sequences related to IS66. The function of these proteins is uncertain, but they are probably essential for transposition.


Pssm-ID: 461723  Cd Length: 96  Bit Score: 154.17  E-value: 3.47e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701    13 IAGITDMRCGFNGLASKVQNTLKDDPFSGHIFVFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPV-TREGKVHLTPA 91
Cdd:pfam05717   1 ATGPTDMRKGIDGLAALVQEALGLDPFSGHLFVFRNRRRDRIKILYWDGDGFWLYYKRLERGRFVWPRrAGDGVVELTAA 80

                          90
                  ....*....|....*.
gi 31983701    92 QLSMLLEGIAWPHPKR 107
Cdd:pfam05717  81 QLSWLLEGIDWRRPRA 96
COG3436 COG3436
Transposase [Mobilome: prophages, transposons];
12-102 6.78e-07

Transposase [Mobilome: prophages, transposons];


Pssm-ID: 442662 [Multi-domain]  Cd Length: 416  Bit Score: 46.18  E-value: 6.78e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31983701  12 IIAGITDMRCgFNGLA---SKVQNTLKDDPfsGHIFVFRGRSGKmVKILWADRDGLC---LFAKRLERG----------- 74
Cdd:COG3436  79 VLHEPTDMRK-IDGLAaigEEVSEELDLDP--GALFVFRNRRDK-YKCLYCDGTGLVyapAPAKRLEKGlagpgllahil 154

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 31983701  75 --RFV--WPVTREGK------VHLTPAQLSMLLEGIAW 102
Cdd:COG3436 155 vsKFVdhLPLYRQEEifarlgVELSRSTLLNWVGRAAE 192
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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