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Conserved domains on  [gi|29653682|ref|NP_819374|]
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sulfur carrier protein ThiS [Coxiella burnetii RSA 493]

Protein Classification

sulfur carrier protein ThiS( domain architecture ID 10088150)

ThiS ubiquitin-like sulfur carrier protein is involved in thiamin biosynthesis in bacteria

Gene Symbol:  thiS
Gene Ontology:  GO:0009228
PubMed:  11135670

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ubl_ThiS cd00565
ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine ...
 4-67 4.10e-22

ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine biosynthesis protein (ThiaminS), is a sulfur carrier protein involved in thiamin biosynthesis in prokaryotes. It has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub), and is activated in an ATP-dependent manner by sulfurtransferases, similar to the activation mechanism of Ub-activating enzyme E1. ThiS has common evolutionary origin with Ub-related protein modifiers in eukaryotes, a beta-grasp fold as Ub, and is closely related to proteins MoaD and Urm1.


:

Pssm-ID: 340451 [Multi-domain]  Cd Length: 64  Bit Score: 79.86  E-value: 4.10e-22
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 29653682  4 IYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:cd00565  1 ITVNGEPREVDEGLTLAELLEELGFTPKGVAVELNGEIVPRSEWAETILKDGDRIEIVTFVGGG 64
 
Name Accession Description Interval E-value
Ubl_ThiS cd00565
ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine ...
 4-67 4.10e-22

ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine biosynthesis protein (ThiaminS), is a sulfur carrier protein involved in thiamin biosynthesis in prokaryotes. It has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub), and is activated in an ATP-dependent manner by sulfurtransferases, similar to the activation mechanism of Ub-activating enzyme E1. ThiS has common evolutionary origin with Ub-related protein modifiers in eukaryotes, a beta-grasp fold as Ub, and is closely related to proteins MoaD and Urm1.


Pssm-ID: 340451 [Multi-domain]  Cd Length: 64  Bit Score: 79.86  E-value: 4.10e-22
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 29653682  4 IYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:cd00565  1 ITVNGEPREVDEGLTLAELLEELGFTPKGVAVELNGEIVPRSEWAETILKDGDRIEIVTFVGGG 64
thiS TIGR01683
thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like ...
 4-67 4.04e-20

thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like thiamine biosynthesis protein related to MoaD, a molybdenum cofactor biosynthesis protein. Both proteins are involved in sulfur transfer. ThiS has a conserved Gly-Gly C-terminus that is modified, in reactions requiring ThiI, ThiF, IscS, and a sulfur atom from Cys, into the thiocarboxylate that provides the sulfur for thiazole biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Thiamine]


Pssm-ID: 273754 [Multi-domain]  Cd Length: 64  Bit Score: 74.91  E-value: 4.04e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 29653682    4 IYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:TIGR01683  1 ITVNGEPVEVEDGTTLAALLESLGLDPRRVAVAVNGEIVPRSEWDDTILKEGDRIEIVTFVGGG 64
ThiS COG2104
Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; ...
 2-67 3.67e-18

Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; Sulfur carrier protein ThiS (thiamine biosynthesis) is part of the Pathway/BioSystem: Thiamine biosynthesis


Pssm-ID: 441707 [Multi-domain]  Cd Length: 66  Bit Score: 70.10  E-value: 3.67e-18
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682  2 IDIYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:COG2104  1 MKITVNGEPREVPEGTTLADLLEELGLDPKGVAVAVNGEIVPRSQWASTVLKEGDRVEIVTAVGGG 66
ThiS pfam02597
ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in ...
 6-67 3.82e-10

ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalyzed by IscS. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.


Pssm-ID: 396932 [Multi-domain]  Cd Length: 74  Bit Score: 49.98  E-value: 3.82e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682    6 LNGKLISLAEKMSLKKLLE----KQGVVMGTVAVAVNETVIPGiNYEKIIIQPGDRIEIITAMCGG 67
Cdd:pfam02597 10 LAGKDEELAEGATVAELLEalglRYPELRERVAVAVNGEIVPR-LWLDTPLKDGDEVAIIPPVGGG 74
PRK11840 PRK11840
bifunctional sulfur carrier protein/thiazole synthase protein; Provisional
 2-67 1.96e-08

bifunctional sulfur carrier protein/thiazole synthase protein; Provisional


Pssm-ID: 236998 [Multi-domain]  Cd Length: 326  Bit Score: 48.59  E-value: 1.96e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682    2 IDIYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:PRK11840   1 MRIRLNGEPRQVPAGLTIAALLAELGLAPKKVAVERNLEIVPRSEYGQVALEEGDELEIVHFVGGG 66
 
Name Accession Description Interval E-value
Ubl_ThiS cd00565
ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine ...
 4-67 4.10e-22

ubiquitin-like (Ubl) domain found in sulfur carrier protein ThiS; ThiS, also termed Thiamine biosynthesis protein (ThiaminS), is a sulfur carrier protein involved in thiamin biosynthesis in prokaryotes. It has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub), and is activated in an ATP-dependent manner by sulfurtransferases, similar to the activation mechanism of Ub-activating enzyme E1. ThiS has common evolutionary origin with Ub-related protein modifiers in eukaryotes, a beta-grasp fold as Ub, and is closely related to proteins MoaD and Urm1.


Pssm-ID: 340451 [Multi-domain]  Cd Length: 64  Bit Score: 79.86  E-value: 4.10e-22
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 29653682  4 IYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:cd00565  1 ITVNGEPREVDEGLTLAELLEELGFTPKGVAVELNGEIVPRSEWAETILKDGDRIEIVTFVGGG 64
thiS TIGR01683
thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like ...
 4-67 4.04e-20

thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like thiamine biosynthesis protein related to MoaD, a molybdenum cofactor biosynthesis protein. Both proteins are involved in sulfur transfer. ThiS has a conserved Gly-Gly C-terminus that is modified, in reactions requiring ThiI, ThiF, IscS, and a sulfur atom from Cys, into the thiocarboxylate that provides the sulfur for thiazole biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Thiamine]


Pssm-ID: 273754 [Multi-domain]  Cd Length: 64  Bit Score: 74.91  E-value: 4.04e-20
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 29653682    4 IYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:TIGR01683  1 ITVNGEPVEVEDGTTLAALLESLGLDPRRVAVAVNGEIVPRSEWDDTILKEGDRIEIVTFVGGG 64
ThiS COG2104
Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; ...
 2-67 3.67e-18

Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; Sulfur carrier protein ThiS (thiamine biosynthesis) is part of the Pathway/BioSystem: Thiamine biosynthesis


Pssm-ID: 441707 [Multi-domain]  Cd Length: 66  Bit Score: 70.10  E-value: 3.67e-18
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682  2 IDIYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:COG2104  1 MKITVNGEPREVPEGTTLADLLEELGLDPKGVAVAVNGEIVPRSQWASTVLKEGDRVEIVTAVGGG 66
ThiS pfam02597
ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in ...
 6-67 3.82e-10

ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalyzed by IscS. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.


Pssm-ID: 396932 [Multi-domain]  Cd Length: 74  Bit Score: 49.98  E-value: 3.82e-10
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682    6 LNGKLISLAEKMSLKKLLE----KQGVVMGTVAVAVNETVIPGiNYEKIIIQPGDRIEIITAMCGG 67
Cdd:pfam02597 10 LAGKDEELAEGATVAELLEalglRYPELRERVAVAVNGEIVPR-LWLDTPLKDGDEVAIIPPVGGG 74
PRK11840 PRK11840
bifunctional sulfur carrier protein/thiazole synthase protein; Provisional
 2-67 1.96e-08

bifunctional sulfur carrier protein/thiazole synthase protein; Provisional


Pssm-ID: 236998 [Multi-domain]  Cd Length: 326  Bit Score: 48.59  E-value: 1.96e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682    2 IDIYLNGKLISLAEKMSLKKLLEKQGVVMGTVAVAVNETVIPGINYEKIIIQPGDRIEIITAMCGG 67
Cdd:PRK11840   1 MRIRLNGEPRQVPAGLTIAALLAELGLAPKKVAVERNLEIVPRSEYGQVALEEGDELEIVHFVGGG 66
MoaD COG1977
Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; ...
 11-67 7.57e-03

Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; Molybdopterin synthase sulfur carrier subunit MoaD is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 441580 [Multi-domain]  Cd Length: 82  Bit Score: 31.71  E-value: 7.57e-03
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 29653682 11 ISLAEKMSLKKLLE---------KQGVVMGTVAVAVNETVIPginyEKIIIQPGDRIEIITAMCGG 67
Cdd:COG1977 21 VELPEGATVGDLLEalaarypglAERLLRRRVRVAVNGEDVR----LDTPLKDGDEVALFPPVAGG 82
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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