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Conserved domains on  [gi|25151365|ref|NP_741145|]
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Myosin, essential light chain [Caenorhabditis elegans]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 1000080)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PTZ00184 super family cl33172
calmodulin; Provisional
 10-142 4.60e-23

calmodulin; Provisional


The actual alignment was detected with superfamily member PTZ00184:

Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 88.28  E-value: 4.60e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   10 KEIFNLYDEELDGKIDGTQVGDVARAAGLKPTQAMVTKAAgQEFKRKGEKRLTFEEWLPMYEQlaKEKEQGTYADFYEGL 89
Cdd:PTZ00184  14 KEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI-NEVDADGNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAF 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 25151365   90 KVFDKEETGKILAAELRHILLALGERLSADEADELLKGVE-DGEGMVKYEDFIK 142
Cdd:PTZ00184  91 KVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADvDGDGQINYEEFVK 144
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 10-142 4.60e-23

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 88.28  E-value: 4.60e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   10 KEIFNLYDEELDGKIDGTQVGDVARAAGLKPTQAMVTKAAgQEFKRKGEKRLTFEEWLPMYEQlaKEKEQGTYADFYEGL 89
Cdd:PTZ00184  14 KEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI-NEVDADGNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAF 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 25151365   90 KVFDKEETGKILAAELRHILLALGERLSADEADELLKGVE-DGEGMVKYEDFIK 142
Cdd:PTZ00184  91 KVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADvDGDGQINYEEFVK 144
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 90-142 2.80e-05

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 39.84  E-value: 2.80e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 25151365  90 KVFDKEETGKILAAELRHILLALGERLSADEADELLKGV-EDGEGMVKYEDFIK 142
Cdd:cd00051   7 RLFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVdKDGDGKIDFEEFLE 60
EF-hand_8 pfam13833
EF-hand domain pair;
96-141 1.87e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 37.29  E-value: 1.87e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 25151365    96 ETGKILAAELRHILLALGER-LSADEADELLKGV-EDGEGMVKYEDFI 141
Cdd:pfam13833   1 EKGVITREELKRALALLGLKdLSEDEVDILFREFdTDGDGYISFDEFC 48
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
9-151 5.34e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 37.85  E-value: 5.34e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   9 LKEIFNLYDEELDGKIDGTQVGDVARAAglkpTQAMVTKAAgqefkRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEg 88
Cdd:COG5126   7 LDRRFDLLDADGDGVLERDDFEALFRRL----WATLFSEAD-----TDGDGRISREEFVAGMESLFEATVEPFARAAFD- 76

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 25151365  89 lkVFDKEETGKILAAELRHILLALGerLSADEADELLKGVE-DGEGMVKYEDFIKKVLAGPFPD 151
Cdd:COG5126  77 --LLDTDGDGKISADEFRRLLTALG--VSEEEADELFARLDtDGDGKISFEEFVAAVRDYYTPD 136
 
Name Accession Description Interval E-value
PTZ00184 PTZ00184
calmodulin; Provisional
 10-142 4.60e-23

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 88.28  E-value: 4.60e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   10 KEIFNLYDEELDGKIDGTQVGDVARAAGLKPTQAMVTKAAgQEFKRKGEKRLTFEEWLPMYEQlaKEKEQGTYADFYEGL 89
Cdd:PTZ00184  14 KEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI-NEVDADGNGTIDFPEFLTLMAR--KMKDTDSEEEIKEAF 90

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 25151365   90 KVFDKEETGKILAAELRHILLALGERLSADEADELLKGVE-DGEGMVKYEDFIK 142
Cdd:PTZ00184  91 KVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADvDGDGQINYEEFVK 144
PTZ00183 PTZ00183
centrin; Provisional
 9-142 7.48e-09

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 51.61  E-value: 7.48e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365    9 LKEIFNLYDEELDGKIDGTQVGDVARAAGLKPTQAMVTKAAgQEFKRKGEKRLTFEEWLPMYEQLAKEKEqgTYADFYEG 88
Cdd:PTZ00183  19 IREAFDLFDTDGSGTIDPKELKVAMRSLGFEPKKEEIKQMI-ADVDKDGSGKIDFEEFLDIMTKKLGERD--PREEILKA 95

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 25151365   89 LKVFDKEETGKILAAELRHILLALGERLSADEADELLKGVE-DGEGMVKYEDFIK 142
Cdd:PTZ00183  96 FRLFDDDKTGKISLKNLKRVAKELGETITDEELQEMIDEADrNGDGEISEEEFYR 150
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 90-142 2.80e-05

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 39.84  E-value: 2.80e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 25151365  90 KVFDKEETGKILAAELRHILLALGERLSADEADELLKGV-EDGEGMVKYEDFIK 142
Cdd:cd00051   7 RLFDKDGDGTISADELKAALKSLGEGLSEEEIDEMIREVdKDGDGKIDFEEFLE 60
EF-hand_8 pfam13833
EF-hand domain pair;
96-141 1.87e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 37.29  E-value: 1.87e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 25151365    96 ETGKILAAELRHILLALGER-LSADEADELLKGV-EDGEGMVKYEDFI 141
Cdd:pfam13833   1 EKGVITREELKRALALLGLKdLSEDEVDILFREFdTDGDGYISFDEFC 48
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
9-151 5.34e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 37.85  E-value: 5.34e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   9 LKEIFNLYDEELDGKIDGTQVGDVARAAglkpTQAMVTKAAgqefkRKGEKRLTFEEWLPMYEQLAKEKEQGTYADFYEg 88
Cdd:COG5126   7 LDRRFDLLDADGDGVLERDDFEALFRRL----WATLFSEAD-----TDGDGRISREEFVAGMESLFEATVEPFARAAFD- 76

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 25151365  89 lkVFDKEETGKILAAELRHILLALGerLSADEADELLKGVE-DGEGMVKYEDFIKKVLAGPFPD 151
Cdd:COG5126  77 --LLDTDGDGKISADEFRRLLTALG--VSEEEADELFARLDtDGDGKISFEEFVAAVRDYYTPD 136
EFh_PEF cd15897
The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five ...
 9-141 1.12e-03

The penta-EF hand (PEF) family; The penta-EF hand (PEF) family contains a group of five EF-hand calcium-binding proteins, including several classical calpain large catalytic subunits (CAPN1, 2, 3, 8, 9, 11, 12, 13, 14), two calpain small subunits (CAPNS1 and CAPNS2), as well as non-calpain PEF proteins, ALG-2 (apoptosis-linked gene 2, also termed programmed cell death protein 6, PDCD6), peflin, sorcin, and grancalcin. Based on the sequence similarity of EF1 hand, ALG-2 and peflin have been classified into group I PEF proteins. Calcium-dependent protease calpain subfamily members, sorcin and grancalcin, are group II PEF proteins. Calpains (EC 3.4.22.17) are calcium-activated intracellular cysteine proteases that play important roles in the degradation or functional modulation in a variety of substrates. They have been implicated in a number of physiological processes such as cell cycle progression, remodeling of cytoskeletal-cell membrane attachments, signal transduction, gene expression and apoptosis. ALG-2 is a pro-apoptotic factor that forms a homodimer in the cell or a heterodimer with its closest paralog peflin through their EF5s. Peflin is a 30-kD PEF protein with a longer N-terminal hydrophobic domain than any other member of the PEF family, and it contains nine nonapeptide (A/PPGGPYGGP) repeats. It exists only as a heterodimer with ALG-2. The dissociation of heterodimer occurs in the presence of Ca2+. ALG-2 interacts with various proteins in a Ca2+-dependent manner. Sorcin (for soluble resistance-related calcium binding protein) is a soluble resistance-related calcium-binding protein that participates in the regulation of calcium homeostasis in cells. Grancalcin is a cytosolic Ca2+-binding protein specifically expressed in neutrophils and monocytes/macrophages. It plays a key role in leukocyte-specific functions that are responsible for host defense. Grancalcin can form a heterodimer together with sorcin. Members in this family contain five EF-hand motifs attached to an N-terminal region of variable length containing one or more short Gly/Pro-rich sequences. These proteins form homodimers or heterodimers through pairing between the 5th EF-hands from the two molecules. Unlike calmodulin, the PEF domains do not undergo major conformational changes upon binding Ca2+.


Pssm-ID: 320054 [Multi-domain]  Cd Length: 165  Bit Score: 37.41  E-value: 1.12e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 25151365   9 LKEIFNLYDEElDGKIDGTQVGDVARAAGLKPTQAMVTKAAGQE----FKRKGEKRLTFEEWLPMYEQLAkekeqgtyaD 84
Cdd:cd15897   2 LRNVFQAVAGD-DGEISATELQQALSNVGWTHFDLGFSLETCRSmiamMDRDHSGKLNFSEFKGLWNYIK---------A 71

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 25151365  85 FYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLKGVEDGEGMVKYEDFI 141
Cdd:cd15897  72 WQEIFRTYDTDGSGTIDSNELRQALSGAGYRLSEQTYDIIIRRYDRGRGNIDFDDFI 128
PTZ00184 PTZ00184
calmodulin; Provisional
 69-141 3.05e-03

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 35.89  E-value: 3.05e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 25151365   69 MYEQLAKEkeqgTYADFYEGLKVFDKEETGKILAAELRHILLALGERLSADEADELLKGVE-DGEGMVKYEDFI 141
Cdd:PTZ00184   1 MADQLTEE----QIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDaDGNGTIDFPEFL 70
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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