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Conserved domains on  [gi|24655771|ref|NP_728695|]
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mutator 2, isoform B [Drosophila melanogaster]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MU2_FHA super family cl39576
Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain ...
 5-98 8.26e-44

Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain found in Drosophila mutator 2 (MU2) protein. FHA domains are generally phosphothreonine (pThr) specific-binding domains and are present in DNA repair and checkpoint proteins. However, phosphothreonine binding is not conserved in the MU2 active site pocket due to the absence of three key residues needed for pThr binding. Dimerization, is conserved between FHA domains of Drosophila MU2 and human MDC1 albeit through different interfaces. The MU2 FHA domain dimerizes via the beta-sheet 2, the MDC1 FHA domain dimerizes via the opposite beta-sheet 1.


The actual alignment was detected with superfamily member pfam18221:

Pssm-ID: 375648  Cd Length: 94  Bit Score: 153.80  E-value: 8.26e-44
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771      5 SLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGK 84
Cdd:pfam18221    1 SLSVNGKKAVLLEPDTIYRIGRKLGLEFFIDDESMELAHATAVILRAGVVRVAAATGKVFVNGLETGNADISEKDAIDGK 80

                           90
                   ....*....|....
gi 24655771     85 VKLRFGNVEARLEF 98
Cdd:pfam18221   81 VKLRFGNVEAELQF 94
BRCT_MDC1_rpt1 cd17744
first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; ...
 1067-1134 2.34e-13

first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the first BRCT domain.


:

Pssm-ID: 349375 [Multi-domain]  Cd Length: 72  Bit Score: 66.10  E-value: 2.34e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 24655771 1067 IKVAFTMCN-RPALETVLKSLKHvvEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKK 1134
Cdd:cd17744    1 PRVLFTGVSdKEEGEKIIKKLGG--SVVDSVEDCTHLVTDKVRRTVKFLCALARGIPIVSPDWLEASIK 67
BRCT_PAXIP1_rpt6_like cd18432
sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA ...
 1168-1250 1.10e-11

sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; PAXIP1, also termed PAX transactivation activation domain-interacting protein (PTIP), is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. It also facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. PAXIP1 contains six BRCT repeats. MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the sixth BRCT domain of PAXIP1 and the second BRCT domain of MDC1.


:

Pssm-ID: 349385 [Multi-domain]  Cd Length: 85  Bit Score: 61.83  E-value: 1.10e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771 1168 LLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISV---DLYVVTTSKDTKSKRRLNNyEKVHFIKTEAVMQAL 1244
Cdd:cd18432    1 LFEGYTFYVTPNVKPPPEELKEIIEAAGGKVLKKLPTKRIEDddpDTIVISCEEDRKLWKPLKK-KGIPIYSVEFILTGI 79


                 ....*.
gi 24655771 1245 VQHNIE 1250
Cdd:cd18432   80 LRQKLD 85
 
Name Accession Description Interval E-value
MU2_FHA pfam18221
Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain ...
 5-98 8.26e-44

Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain found in Drosophila mutator 2 (MU2) protein. FHA domains are generally phosphothreonine (pThr) specific-binding domains and are present in DNA repair and checkpoint proteins. However, phosphothreonine binding is not conserved in the MU2 active site pocket due to the absence of three key residues needed for pThr binding. Dimerization, is conserved between FHA domains of Drosophila MU2 and human MDC1 albeit through different interfaces. The MU2 FHA domain dimerizes via the beta-sheet 2, the MDC1 FHA domain dimerizes via the opposite beta-sheet 1.


Pssm-ID: 375648  Cd Length: 94  Bit Score: 153.80  E-value: 8.26e-44
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771      5 SLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGK 84
Cdd:pfam18221    1 SLSVNGKKAVLLEPDTIYRIGRKLGLEFFIDDESMELAHATAVILRAGVVRVAAATGKVFVNGLETGNADISEKDAIDGK 80

                           90
                   ....*....|....
gi 24655771     85 VKLRFGNVEARLEF 98
Cdd:pfam18221   81 VKLRFGNVEAELQF 94
BRCT_MDC1_rpt1 cd17744
first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; ...
 1067-1134 2.34e-13

first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the first BRCT domain.


Pssm-ID: 349375 [Multi-domain]  Cd Length: 72  Bit Score: 66.10  E-value: 2.34e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 24655771 1067 IKVAFTMCN-RPALETVLKSLKHvvEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKK 1134
Cdd:cd17744    1 PRVLFTGVSdKEEGEKIIKKLGG--SVVDSVEDCTHLVTDKVRRTVKFLCALARGIPIVSPDWLEASIK 67
BRCT_PAXIP1_rpt6_like cd18432
sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA ...
 1168-1250 1.10e-11

sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; PAXIP1, also termed PAX transactivation activation domain-interacting protein (PTIP), is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. It also facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. PAXIP1 contains six BRCT repeats. MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the sixth BRCT domain of PAXIP1 and the second BRCT domain of MDC1.


Pssm-ID: 349385 [Multi-domain]  Cd Length: 85  Bit Score: 61.83  E-value: 1.10e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771 1168 LLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISV---DLYVVTTSKDTKSKRRLNNyEKVHFIKTEAVMQAL 1244
Cdd:cd18432    1 LFEGYTFYVTPNVKPPPEELKEIIEAAGGKVLKKLPTKRIEDddpDTIVISCEEDRKLWKPLKK-KGIPIYSVEFILTGI 79


                 ....*.
gi 24655771 1245 VQHNIE 1250
Cdd:cd18432   80 LRQKLD 85
RTT107_BRCT_5 pfam16770
Regulator of Ty1 transposition protein 107 BRCT domain; This is the fifth BRCT domain of ...
 1059-1145 2.92e-05

Regulator of Ty1 transposition protein 107 BRCT domain; This is the fifth BRCT domain of regulator of Ty1 transposition protein 107 (RTT107). It is involved in binding phosphorylated histone H2A.


Pssm-ID: 465266  Cd Length: 91  Bit Score: 43.66  E-value: 2.92e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771   1059 KAKTTTGKIKVAFTMCNRPAL--ETVLKSLKHV-VEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKKT 1135
Cdd:pfam16770    1 KKSLPPYDIRAVLTGCERWIDkeDLDKKKLRLLgIKIVQDPSKCNHLIAPKILRTEKFLCALAFAPYILSPDFITDCLKE 80

                           90
                   ....*....|
gi 24655771   1136 RSIDIKADHL 1145
Cdd:pfam16770   81 GKLPDEEDYL 90
 
Name Accession Description Interval E-value
MU2_FHA pfam18221
Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain ...
 5-98 8.26e-44

Mutator 2 Fork head associated domain; This is the N-terminal forkhead-associated (FHA) domain found in Drosophila mutator 2 (MU2) protein. FHA domains are generally phosphothreonine (pThr) specific-binding domains and are present in DNA repair and checkpoint proteins. However, phosphothreonine binding is not conserved in the MU2 active site pocket due to the absence of three key residues needed for pThr binding. Dimerization, is conserved between FHA domains of Drosophila MU2 and human MDC1 albeit through different interfaces. The MU2 FHA domain dimerizes via the beta-sheet 2, the MDC1 FHA domain dimerizes via the opposite beta-sheet 1.


Pssm-ID: 375648  Cd Length: 94  Bit Score: 153.80  E-value: 8.26e-44
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771      5 SLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGK 84
Cdd:pfam18221    1 SLSVNGKKAVLLEPDTIYRIGRKLGLEFFIDDESMELAHATAVILRAGVVRVAAATGKVFVNGLETGNADISEKDAIDGK 80

                           90
                   ....*....|....
gi 24655771     85 VKLRFGNVEARLEF 98
Cdd:pfam18221   81 VKLRFGNVEAELQF 94
BRCT_MDC1_rpt1 cd17744
first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; ...
 1067-1134 2.34e-13

first BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the first BRCT domain.


Pssm-ID: 349375 [Multi-domain]  Cd Length: 72  Bit Score: 66.10  E-value: 2.34e-13
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 24655771 1067 IKVAFTMCN-RPALETVLKSLKHvvEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKK 1134
Cdd:cd17744    1 PRVLFTGVSdKEEGEKIIKKLGG--SVVDSVEDCTHLVTDKVRRTVKFLCALARGIPIVSPDWLEASIK 67
BRCT_PAXIP1_rpt6_like cd18432
sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA ...
 1168-1250 1.10e-11

sixth BRCT domain of PAX-interacting protein 1 (PAXIP1), second BRCT domain of mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; PAXIP1, also termed PAX transactivation activation domain-interacting protein (PTIP), is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. It also facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. PAXIP1 contains six BRCT repeats. MDC1, also termed nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The family corresponds to the sixth BRCT domain of PAXIP1 and the second BRCT domain of MDC1.


Pssm-ID: 349385 [Multi-domain]  Cd Length: 85  Bit Score: 61.83  E-value: 1.10e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771 1168 LLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISV---DLYVVTTSKDTKSKRRLNNyEKVHFIKTEAVMQAL 1244
Cdd:cd18432    1 LFEGYTFYVTPNVKPPPEELKEIIEAAGGKVLKKLPTKRIEDddpDTIVISCEEDRKLWKPLKK-KGIPIYSVEFILTGI 79


                 ....*.
gi 24655771 1245 VQHNIE 1250
Cdd:cd18432   80 LRQKLD 85
BRCT_BRC1_like_rpt5 cd17743
fifth BRCT domain of Schizosaccharomyces pombe BRCT-containing protein 1 (BRC1) and similar ...
 1067-1129 8.87e-08

fifth BRCT domain of Schizosaccharomyces pombe BRCT-containing protein 1 (BRC1) and similar proteins; Schizosaccharomyces pombe BRC1 is required for mitotic fidelity, specifically in the G2 phase of the cell cycle. It plays a role in chromatin organization. The family also includes Cryptococcus neoformans DNA ligase 4 (LIG4, also known as DNA ligase IV or polydeoxyribonucleotide synthase [ATP] 4), which is involved in dsDNA break repair, and plays a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining. Members in this family contain six BRCT domains. This family corresponds to the fifth one.


Pssm-ID: 349374  Cd Length: 70  Bit Score: 50.32  E-value: 8.87e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 24655771 1067 IKVAFTMCNRPALETVLKSLKHVVEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWL 1129
Cdd:cd17743    1 VRLLFTGYKLWTEKEIKKLKKLGISIVEDPDECTHLVAPKIVRTEKFLCALAYAPVIVTTDWL 63
BRCT_PAXIP1_rpt5 cd17712
fifth BRCT domain of PAX-interacting protein 1 (PAXIP1) and similar proteins; PAXIP1, also ...
 1091-1130 1.40e-05

fifth BRCT domain of PAX-interacting protein 1 (PAXIP1) and similar proteins; PAXIP1, also termed PAX transactivation activation domain-interacting protein (PTIP), is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. It also facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. PAXIP1 contains six BRCT repeats. This family corresponds to the fifth BRCT domain.


Pssm-ID: 349344  Cd Length: 75  Bit Score: 44.15  E-value: 1.40e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 24655771 1091 EITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLH 1130
Cdd:cd17712   27 EVVESPQECTHLVAPKVSRTVKFLTAISVCKHIVTPEWLE 66
RTT107_BRCT_5 pfam16770
Regulator of Ty1 transposition protein 107 BRCT domain; This is the fifth BRCT domain of ...
 1059-1145 2.92e-05

Regulator of Ty1 transposition protein 107 BRCT domain; This is the fifth BRCT domain of regulator of Ty1 transposition protein 107 (RTT107). It is involved in binding phosphorylated histone H2A.


Pssm-ID: 465266  Cd Length: 91  Bit Score: 43.66  E-value: 2.92e-05
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24655771   1059 KAKTTTGKIKVAFTMCNRPAL--ETVLKSLKHV-VEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKKT 1135
Cdd:pfam16770    1 KKSLPPYDIRAVLTGCERWIDkeDLDKKKLRLLgIKIVQDPSKCNHLIAPKILRTEKFLCALAFAPYILSPDFITDCLKE 80

                           90
                   ....*....|
gi 24655771   1136 RSIDIKADHL 1145
Cdd:pfam16770   81 GKLPDEEDYL 90
BRCT_PAXIP1_rpt6 cd18440
sixth BRCT domain of PAX-interacting protein 1 (PAXIP1) and similar proteins; PAXIP1, also ...
 1168-1206 3.49e-03

sixth BRCT domain of PAX-interacting protein 1 (PAXIP1) and similar proteins; PAXIP1, also termed PAX transactivation activation domain-interacting protein (PTIP), is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. It also facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. PAXIP1 contains six BRCT repeats. This family corresponds to the sixth BRCT domain. The Trp-X-X-X-Cys/Ser signature motif of the BRCT family is not conserved in this family.


Pssm-ID: 349393  Cd Length: 90  Bit Score: 37.97  E-value: 3.49e-03
                         10        20        30
                 ....*....|....*....|....*....|....*....
gi 24655771 1168 LLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLA 1206
Cdd:cd18440    1 LFKGKYFYITPGVKPSPSVLKEIIESAGGKVEKKRPSLK 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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