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Conserved domains on  [gi|186515274|ref|NP_680753|]
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Transcriptional factor B3 family protein [Arabidopsis thaliana]

Protein Classification

B3 domain-containing protein( domain architecture ID 10659976)

B3 DNA-binding domain-containing protein such as Arabidopsis thaliana REM1 that may play a role in flower development

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 151-245 4.91e-27

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 104.30  E-value: 4.91e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   151 YLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSFYR 230
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80

                           90
                   ....*....|....*
gi 186515274   231 FKLVQNGErPLLRLC 245
Cdd:smart01019  81 FELDGNGE-FVVSIF 94
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 274-371 1.77e-22

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 91.59  E-value: 1.77e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   274 FLTMTFKPYMLKSGQLRLSRPFAIENEIKE-AEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGETF 352
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKL-KYRGSSGMFLLTGGWKEFVKANGLKAGDSL 79

                           90
                   ....*....|....*....
gi 186515274   353 TLEFVRGEgtTPMLKFCSK 371
Cdd:smart01019  80 TFELDGNG--EFVVSIFRK 96
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 14-103 1.39e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 66.55  E-value: 1.39e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274    14 FFVIVLHGH---KSSPMIPAEFFSTYVEGKNHQstKLKLTSDAfDRTWEVKLNGRR------FAGGWENFSTVHSLQDDD 84
Cdd:smart01019   1 FFKTLTTSDvskHGRLVLPKQFAESNGPPLKPK--EITLLDPD-GKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGD 77

                           90
                   ....*....|....*....
gi 186515274    85 VVIFREIGDMTFHVTASGR 103
Cdd:smart01019  78 SLTFELDGNGEFVVSIFRK 96
 
Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 151-245 4.91e-27

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 104.30  E-value: 4.91e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   151 YLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSFYR 230
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80

                           90
                   ....*....|....*
gi 186515274   231 FKLVQNGErPLLRLC 245
Cdd:smart01019  81 FELDGNGE-FVVSIF 94
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 274-371 1.77e-22

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 91.59  E-value: 1.77e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   274 FLTMTFKPYMLKSGQLRLSRPFAIENEIKE-AEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGETF 352
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKL-KYRGSSGMFLLTGGWKEFVKANGLKAGDSL 79

                           90
                   ....*....|....*....
gi 186515274   353 TLEFVRGEgtTPMLKFCSK 371
Cdd:smart01019  80 TFELDGNG--EFVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 274-372 2.55e-22

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 91.28  E-value: 2.55e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  274 FLTMTFKPYMLKSGQLRLSRPFAIEN---EIKEAEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGE 350
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsglPKRLSKEITLLDEHGKSWTVKL-RYRKKSGRYVLTGGWKEFVKANGLKAGD 79

                          90       100
                  ....*....|....*....|..
gi 186515274  351 TFTLEFVRGEGTTPMLKFCSKS 372
Cdd:pfam02362  80 FLTFKLVGNSGFTPVVGIFRKS 101
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 151-245 1.32e-20

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 86.66  E-value: 1.32e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  151 YLVAHVTPSNLSRDRLYLIKAFARSN-GLNERCC-EIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSF 228
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsGLPKRLSkEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLKAGDF 80

                          90
                  ....*....|....*...
gi 186515274  229 YRFKLVQNGE-RPLLRLC 245
Cdd:pfam02362  81 LTFKLVGNSGfTPVVGIF 98
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 151-245 4.41e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 85.07  E-value: 4.41e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274 151 YLVAHVTPSNLSR-DRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSFY 229
Cdd:cd10017    2 LFFKVLTPSDVSSlGRLVLPKKFAKAHLPPKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81

                         90
                 ....*....|....*.
gi 186515274 230 RFKLVQNGERPLLRLC 245
Cdd:cd10017   82 VFELDGRNSKFKVRIF 97
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 274-362 1.71e-15

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 71.98  E-value: 1.71e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274 274 FLTMTfKPYMLKSGQLRLSRPFAIEN-EIKEAEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGETF 352
Cdd:cd10017    4 FKVLT-PSDVSSLGRLVLPKKFAKAHlPPKEGVEVTLEDPDGKKWTVKL-KKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81

                         90
                 ....*....|
gi 186515274 353 TLEFVRGEGT 362
Cdd:cd10017   82 VFELDGRNSK 91
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 14-103 1.39e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 66.55  E-value: 1.39e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274    14 FFVIVLHGH---KSSPMIPAEFFSTYVEGKNHQstKLKLTSDAfDRTWEVKLNGRR------FAGGWENFSTVHSLQDDD 84
Cdd:smart01019   1 FFKTLTTSDvskHGRLVLPKQFAESNGPPLKPK--EITLLDPD-GKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGD 77

                           90
                   ....*....|....*....
gi 186515274    85 VVIFREIGDMTFHVTASGR 103
Cdd:smart01019  78 SLTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 12-99 3.19e-11

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 59.65  E-value: 3.19e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  12 KHFFVIVLHGHKSS---PMIPAEFFSTYVEGKNHQSTKLKltsDAFDRTWEVKLNGR------RFAGGWENFSTVHSLQD 82
Cdd:cd10017    1 PLFFKVLTPSDVSSlgrLVLPKKFAKAHLPPKEGVEVTLE---DPDGKKWTVKLKKRknsgrmVLSGGWKEFVRANGLKE 77

                         90
                 ....*....|....*...
gi 186515274  83 DDVVIFREIG-DMTFHVT 99
Cdd:cd10017   78 GDFLVFELDGrNSKFKVR 95
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 28-96 5.03e-07

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 47.75  E-value: 5.03e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 186515274   28 IPAEFFSTYVEGKNHQSTKLKLTsDAFDRTWEVKLNGRR------FAGGWENFSTVHSLQDDDVVIFREIGDMTF 96
Cdd:pfam02362  18 IPKKFARENSGLPKRLSKEITLL-DEHGKSWTVKLRYRKksgryvLTGGWKEFVKANGLKAGDFLTFKLVGNSGF 91
 
Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 151-245 4.91e-27

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 104.30  E-value: 4.91e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   151 YLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSFYR 230
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80

                           90
                   ....*....|....*
gi 186515274   231 FKLVQNGErPLLRLC 245
Cdd:smart01019  81 FELDGNGE-FVVSIF 94
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 274-371 1.77e-22

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 91.59  E-value: 1.77e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274   274 FLTMTFKPYMLKSGQLRLSRPFAIENEIKE-AEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGETF 352
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKL-KYRGSSGMFLLTGGWKEFVKANGLKAGDSL 79

                           90
                   ....*....|....*....
gi 186515274   353 TLEFVRGEgtTPMLKFCSK 371
Cdd:smart01019  80 TFELDGNG--EFVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 274-372 2.55e-22

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 91.28  E-value: 2.55e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  274 FLTMTFKPYMLKSGQLRLSRPFAIEN---EIKEAEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGE 350
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsglPKRLSKEITLLDEHGKSWTVKL-RYRKKSGRYVLTGGWKEFVKANGLKAGD 79

                          90       100
                  ....*....|....*....|..
gi 186515274  351 TFTLEFVRGEGTTPMLKFCSKS 372
Cdd:pfam02362  80 FLTFKLVGNSGFTPVVGIFRKS 101
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 151-245 1.32e-20

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 86.66  E-value: 1.32e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  151 YLVAHVTPSNLSRDRLYLIKAFARSN-GLNERCC-EIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSF 228
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsGLPKRLSkEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLKAGDF 80

                          90
                  ....*....|....*...
gi 186515274  229 YRFKLVQNGE-RPLLRLC 245
Cdd:pfam02362  81 LTFKLVGNSGfTPVVGIF 98
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 151-245 4.41e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 85.07  E-value: 4.41e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274 151 YLVAHVTPSNLSR-DRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLRYSTVNDQASISGGWRDFCLVNGLEAGSFY 229
Cdd:cd10017    2 LFFKVLTPSDVSSlGRLVLPKKFAKAHLPPKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81

                         90
                 ....*....|....*.
gi 186515274 230 RFKLVQNGERPLLRLC 245
Cdd:cd10017   82 VFELDGRNSKFKVRIF 97
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 274-362 1.71e-15

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 71.98  E-value: 1.71e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274 274 FLTMTfKPYMLKSGQLRLSRPFAIEN-EIKEAEDITLVDKNGGNWPSYVaSGDGQGGKYYLAKGWRSFCAANRLKTGETF 352
Cdd:cd10017    4 FKVLT-PSDVSSLGRLVLPKKFAKAHlPPKEGVEVTLEDPDGKKWTVKL-KKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81

                         90
                 ....*....|
gi 186515274 353 TLEFVRGEGT 362
Cdd:cd10017   82 VFELDGRNSK 91
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 14-103 1.39e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 66.55  E-value: 1.39e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274    14 FFVIVLHGH---KSSPMIPAEFFSTYVEGKNHQstKLKLTSDAfDRTWEVKLNGRR------FAGGWENFSTVHSLQDDD 84
Cdd:smart01019   1 FFKTLTTSDvskHGRLVLPKQFAESNGPPLKPK--EITLLDPD-GKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGD 77

                           90
                   ....*....|....*....
gi 186515274    85 VVIFREIGDMTFHVTASGR 103
Cdd:smart01019  78 SLTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 12-99 3.19e-11

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 59.65  E-value: 3.19e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 186515274  12 KHFFVIVLHGHKSS---PMIPAEFFSTYVEGKNHQSTKLKltsDAFDRTWEVKLNGR------RFAGGWENFSTVHSLQD 82
Cdd:cd10017    1 PLFFKVLTPSDVSSlgrLVLPKKFAKAHLPPKEGVEVTLE---DPDGKKWTVKLKKRknsgrmVLSGGWKEFVRANGLKE 77

                         90
                 ....*....|....*...
gi 186515274  83 DDVVIFREIG-DMTFHVT 99
Cdd:cd10017   78 GDFLVFELDGrNSKFKVR 95
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 28-96 5.03e-07

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 47.75  E-value: 5.03e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 186515274   28 IPAEFFSTYVEGKNHQSTKLKLTsDAFDRTWEVKLNGRR------FAGGWENFSTVHSLQDDDVVIFREIGDMTF 96
Cdd:pfam02362  18 IPKKFARENSGLPKRLSKEITLL-DEHGKSWTVKLRYRKksgryvLTGGWKEFVKANGLKAGDFLTFKLVGNSGF 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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