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Conserved domains on  [gi|221499783|ref|NP_608361|]
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THADA, isoform B [Drosophila melanogaster]

Protein Classification

THADA/TRM732 family protein( domain architecture ID 10563587)

thyroid adenoma-associated protein (THADA)/TRM732 family protein is a DUF2428 domain-containing protein; similar to Homo sapiens thyroid adenoma-associated protein (THADA) and the tRNA -methyltransferase non-catalytic subunit TRM732

Gene Ontology:  GO:0030488
PubMed:  17889454

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
DUF2428 pfam10350
THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins ...
863-1113 4.18e-73

THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins (THADA) and the yeast homolog, TRM732. Trm732 forms a complex with the methyltransferase Trm7 to 2'- O-methylate tRNA residue 32 (Nm32), being required for Trm7 methylation activity. In humans, mutations of the Trm7 homolog FTSJ1, which interacts with THADA, impair Nm32 modifications, associated with non-syndromic X-linked intellectual disability. It has been suggested that these proteins may play a role in additional biological processes not related to translation. This domain contains a RRSAGLP conserved motif that is required for tRNA modification activity (Funk HM et.al., Preprint from bioRxiv, 03 Jun 2021 DOI: 10.1101/2021.06.03.446962).


:

Pssm-ID: 463054 [Multi-domain]  Cd Length: 260  Bit Score: 244.96  E-value: 4.18e-73
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   863 PEGHLPETCDQETDQpltnvldrQLSREELLQVRTTPQMILLCAWRSSKEVCLILGELVQRAPLEEEEDEeqqqqqGDFL 942
Cdd:pfam10350   26 PEGHLPEDLDDMDDD--------STIDESEETEGLTHQILLSCSWRALKESSLLLGTIVKRVPLTTSPSN------GDQL 91
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   943 LSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFTMLCRRFWHSESVRLNQLPGQWVDEAMAMVSGQeewagkgaRLCATR 1022
Cdd:pfam10350   92 LTVEQLERIGELFFTQLLELRHRGAFETVYVGFTALCRRLLSSDDPELKQLPEQWLQELLEAIKSS--------SLSITR 163
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  1023 RSAGMPFMLQALVGTELKLGTHATLYRCMNRLLEVCER-------RTGGAAGITARSHALNIMRALFRSSELAELVTEFM 1095
Cdd:pfam10350  164 RSAGLPFLILAILSSEPNSGKRPLLHRAMKELLEIATSpvssdeeSESDETVDLPQVHALNILRALFRDSLLGERVSPYV 243
                          250
                   ....*....|....*...
gi 221499783  1096 ARGIQCALDGlLLAEEWA 1113
Cdd:pfam10350  244 ADGLILAIEG-FSSPVWA 260
 
Name Accession Description Interval E-value
DUF2428 pfam10350
THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins ...
863-1113 4.18e-73

THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins (THADA) and the yeast homolog, TRM732. Trm732 forms a complex with the methyltransferase Trm7 to 2'- O-methylate tRNA residue 32 (Nm32), being required for Trm7 methylation activity. In humans, mutations of the Trm7 homolog FTSJ1, which interacts with THADA, impair Nm32 modifications, associated with non-syndromic X-linked intellectual disability. It has been suggested that these proteins may play a role in additional biological processes not related to translation. This domain contains a RRSAGLP conserved motif that is required for tRNA modification activity (Funk HM et.al., Preprint from bioRxiv, 03 Jun 2021 DOI: 10.1101/2021.06.03.446962).


Pssm-ID: 463054 [Multi-domain]  Cd Length: 260  Bit Score: 244.96  E-value: 4.18e-73
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   863 PEGHLPETCDQETDQpltnvldrQLSREELLQVRTTPQMILLCAWRSSKEVCLILGELVQRAPLEEEEDEeqqqqqGDFL 942
Cdd:pfam10350   26 PEGHLPEDLDDMDDD--------STIDESEETEGLTHQILLSCSWRALKESSLLLGTIVKRVPLTTSPSN------GDQL 91
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   943 LSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFTMLCRRFWHSESVRLNQLPGQWVDEAMAMVSGQeewagkgaRLCATR 1022
Cdd:pfam10350   92 LTVEQLERIGELFFTQLLELRHRGAFETVYVGFTALCRRLLSSDDPELKQLPEQWLQELLEAIKSS--------SLSITR 163
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  1023 RSAGMPFMLQALVGTELKLGTHATLYRCMNRLLEVCER-------RTGGAAGITARSHALNIMRALFRSSELAELVTEFM 1095
Cdd:pfam10350  164 RSAGLPFLILAILSSEPNSGKRPLLHRAMKELLEIATSpvssdeeSESDETVDLPQVHALNILRALFRDSLLGERVSPYV 243
                          250
                   ....*....|....*...
gi 221499783  1096 ARGIQCALDGlLLAEEWA 1113
Cdd:pfam10350  244 ADGLILAIEG-FSSPVWA 260
COG5543 COG5543
Uncharacterized conserved protein [Function unknown];
897-1271 1.07e-22

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227830 [Multi-domain]  Cd Length: 1400  Bit Score: 106.63  E-value: 1.07e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  897 TTPQMILLCAWRSSKEVCLILGELVQRAPLeeeedeeqqqqqgdfllSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFT 976
Cdd:COG5543   643 VPDQVILSVYWRNMKECSSLLCGMLLKYPL-----------------TRGNLALSGRLVQVLLSHIRHLGAFLQVSPYLN 705
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  977 MLCRRFWHSesvrLNQLPGQWVDEAMAMVSGQEEWagkgarlcATRRSAGMPFMLQALVGTELKLGtHATLYRCMNRLLE 1056
Cdd:COG5543   706 MVCKRCNME----FEEDELLELVKVSFNRIEKCKA--------ITRRSGGIPLLFTSILRAEVTKY-PSLLERTLKRLLE 772
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783 1057 VCERRTGGAAGI--TARSHALNIMRALFRSSELAELVTEFMARGIQCALDGlLLAEEWAERNSATLLLAALIVRVFGver 1134
Cdd:COG5543   773 LAETPESSHQDEfdLPQVHALNILSRIFEEGCLSSKLPLQIEEAFGLAFKC-FRSNLWAIRNCSIMLFTNLVNRAFG--- 848
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783 1135 aRLETGELHVRNRMTGRIFFTRYPQLFDYFHAALQRESEQMdaggggsenaSGKRRQAVQLEAMLLMLSRLYPSSLEGae 1214
Cdd:COG5543   849 -RGPEGIVGMAHAGSRKLFFKKLSLLRDKLLDVLALSIAHL----------SGRMRESNGEESIFLVTEALWRLSPRG-- 915
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 221499783 1215 STLNLSEFVPFLIRICHSHDLMTREMAALVvanFVTQEQALAEIRRIVVELKALQLR 1271
Cdd:COG5543   916 GVTGLKSFNICTKKCWKMPNWKARPRASMV---LNPLLSEGEVLLRVLILLDSEAVE 969
 
Name Accession Description Interval E-value
DUF2428 pfam10350
THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins ...
863-1113 4.18e-73

THADA/TRM732, DUF2428; This conserved domain is found in Thyroid adenoma-associated proteins (THADA) and the yeast homolog, TRM732. Trm732 forms a complex with the methyltransferase Trm7 to 2'- O-methylate tRNA residue 32 (Nm32), being required for Trm7 methylation activity. In humans, mutations of the Trm7 homolog FTSJ1, which interacts with THADA, impair Nm32 modifications, associated with non-syndromic X-linked intellectual disability. It has been suggested that these proteins may play a role in additional biological processes not related to translation. This domain contains a RRSAGLP conserved motif that is required for tRNA modification activity (Funk HM et.al., Preprint from bioRxiv, 03 Jun 2021 DOI: 10.1101/2021.06.03.446962).


Pssm-ID: 463054 [Multi-domain]  Cd Length: 260  Bit Score: 244.96  E-value: 4.18e-73
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   863 PEGHLPETCDQETDQpltnvldrQLSREELLQVRTTPQMILLCAWRSSKEVCLILGELVQRAPLEEEEDEeqqqqqGDFL 942
Cdd:pfam10350   26 PEGHLPEDLDDMDDD--------STIDESEETEGLTHQILLSCSWRALKESSLLLGTIVKRVPLTTSPSN------GDQL 91
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783   943 LSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFTMLCRRFWHSESVRLNQLPGQWVDEAMAMVSGQeewagkgaRLCATR 1022
Cdd:pfam10350   92 LTVEQLERIGELFFTQLLELRHRGAFETVYVGFTALCRRLLSSDDPELKQLPEQWLQELLEAIKSS--------SLSITR 163
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  1023 RSAGMPFMLQALVGTELKLGTHATLYRCMNRLLEVCER-------RTGGAAGITARSHALNIMRALFRSSELAELVTEFM 1095
Cdd:pfam10350  164 RSAGLPFLILAILSSEPNSGKRPLLHRAMKELLEIATSpvssdeeSESDETVDLPQVHALNILRALFRDSLLGERVSPYV 243
                          250
                   ....*....|....*...
gi 221499783  1096 ARGIQCALDGlLLAEEWA 1113
Cdd:pfam10350  244 ADGLILAIEG-FSSPVWA 260
COG5543 COG5543
Uncharacterized conserved protein [Function unknown];
897-1271 1.07e-22

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227830 [Multi-domain]  Cd Length: 1400  Bit Score: 106.63  E-value: 1.07e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  897 TTPQMILLCAWRSSKEVCLILGELVQRAPLeeeedeeqqqqqgdfllSRAQLEAIGEHFLQLLAETKHRGAFEQAYVGFT 976
Cdd:COG5543   643 VPDQVILSVYWRNMKECSSLLCGMLLKYPL-----------------TRGNLALSGRLVQVLLSHIRHLGAFLQVSPYLN 705
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783  977 MLCRRFWHSesvrLNQLPGQWVDEAMAMVSGQEEWagkgarlcATRRSAGMPFMLQALVGTELKLGtHATLYRCMNRLLE 1056
Cdd:COG5543   706 MVCKRCNME----FEEDELLELVKVSFNRIEKCKA--------ITRRSGGIPLLFTSILRAEVTKY-PSLLERTLKRLLE 772
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783 1057 VCERRTGGAAGI--TARSHALNIMRALFRSSELAELVTEFMARGIQCALDGlLLAEEWAERNSATLLLAALIVRVFGver 1134
Cdd:COG5543   773 LAETPESSHQDEfdLPQVHALNILSRIFEEGCLSSKLPLQIEEAFGLAFKC-FRSNLWAIRNCSIMLFTNLVNRAFG--- 848
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 221499783 1135 aRLETGELHVRNRMTGRIFFTRYPQLFDYFHAALQRESEQMdaggggsenaSGKRRQAVQLEAMLLMLSRLYPSSLEGae 1214
Cdd:COG5543   849 -RGPEGIVGMAHAGSRKLFFKKLSLLRDKLLDVLALSIAHL----------SGRMRESNGEESIFLVTEALWRLSPRG-- 915
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 221499783 1215 STLNLSEFVPFLIRICHSHDLMTREMAALVvanFVTQEQALAEIRRIVVELKALQLR 1271
Cdd:COG5543   916 GVTGLKSFNICTKKCWKMPNWKARPRASMV---LNPLLSEGEVLLRVLILLDSEAVE 969
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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