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Conserved domains on  [gi|24642191|ref|NP_573030|]
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Grip128 [Drosophila melanogaster]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GCP5_NTD cd22572
N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, ...
 25-130 2.86e-09

N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, also called TUBGCP5, is a component of the gamma-tubulin ring complex (gamma-TuRC), which is necessary for microtubule nucleation at the centrosome. The model corresponds to the N-terminal domain (NTD) of GCP-5, which is involved in protein-protein interactions. GCP-5 binds Mozart1, a microprotein that regulates subcellular targeting and microtubule formation activity of gamma-TuRC at different cell cycle stages, through its NTD.


:

Pssm-ID: 439339  Cd Length: 102  Bit Score: 55.25  E-value: 2.86e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191   25 INENVRKLVRSLSEPGTSQKKLRQMEDTALKIIANQRRTwapTTTDMEVKRNLDDLAERFRAEGMASFGESIKDLAIRYL 104
Cdd:cd22572    1 LEELLEELITALTGFQEDDENFRLCLDFALSNLRYHRFL---STNSFEVERRLDGLVEKFRVHGQDDLADALRELLDELL 77

                         90       100
                 ....*....|....*....|....*.
gi 24642191  105 AMDENQQQfNPNPGWQMLELLFRIAD 130
Cdd:cd22572   78 KLPLFDHS-QSDWHPSILSLLLELSD 102
GCP_C_terminal super family cl38152
Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components ...
 873-1082 1.57e-07

Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Structure-based sequence analysis revealed the existence of an exposed surface area conserved in all human GCPs and in GCP4 orthologs. This area is located in the C-terminal domain of GCP4, which was confirmed in vitro to bind directly to gamma-tubulin. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains.


The actual alignment was detected with superfamily member pfam04130:

Pssm-ID: 461187  Cd Length: 297  Bit Score: 54.17  E-value: 1.57e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    873 ILTPAHMQDYNSVWRLMLKVKWAVWKLENMQFLRRARFNPcapldliGLTIRRLEIVRFWLIFLINNLHAHIMEAVS--- 949
Cdd:pfam04130   95 VLTPEALTKYQRLFRFLLRLKRVEFVLSSLWRRRQMSGSR-------SVLWHRARLLRQEMIHFVSQLQYYVMFEVIeps 167

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    950 -RQFELRIG-ECKNVRELRIMHDEHLAWLKTHCMLTDEFKAFRVALDQIFhlvyvldmewttcaSCMHDHdALCLDVTIS 1027
Cdd:pfam04130  168 wREFEEKLQkAASDLDDLIEAHEDFLDRILKKCFLTSPQQPLLKLLEEIL--------------SLILDF-AEALDGLYL 232

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 24642191   1028 DDGIDDSETQRRSLEYLALNQVAEIELTYIRCHQMLADILNTLVHQNDHGFLSAL 1082
Cdd:pfam04130  233 SVSESARAEAEDELPELERERLRRLEKQFRKKVSLLLKVLRGLKSHPDESHLRQL 287
GCP_N_terminal super family cl40875
Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components ...
 371-574 7.23e-07

Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Functional studies have shown that the N-terminal domain defines the functional identity of GCPs, suggesting that all GCPs are incorporated into the helix of gamma-tubulin small complexes (gTURCs) via lateral interactions between their N-terminal domains. Thereby, they define the direct neighbors and position the GCPs within the helical wall of gTuRC. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains. In addition to the conserved sequences, the N-terminal domains carry specific insertions of various sizes depending on the GCP, i.e. internal insertions or N-terminal extensions. These insertions may equally contribute to the function of individual GCPs as they have been implied in specific interactions with regulatory or structural proteins. For instance, GCP6 carries a large internal insertion phosphorylated by Plk4 and containing a domain of interaction with keratins, whereas the N-terminal extension of GCP3 interacts with the recruitment protein MOZART1.


The actual alignment was detected with superfamily member pfam17681:

Pssm-ID: 465456  Cd Length: 298  Bit Score: 52.29  E-value: 7.23e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    371 LREILFMFV-RPASccFFEFDKATRRITVRDNVSiCTVTASTMQnfLLFDVVPALEDMMELRHIIDTHTLHFHGvkttYT 449
Cdd:pfam17681    1 LRDLLFALQgISGS--YIRFDESDSRIVDDIRIP-GILPPSLRS--LLSRLLELGLLYRRLRKFVESSSSFEYG----LV 71

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    450 VEFFAYGLRDLVQPICQLLIAYEDRVNKDPA-KNTLIRFTIEFREHFSQLRLLRELAEDVILdkgpAHIRSAYLLSRLYK 528
Cdd:pfam17681   72 LQALCAALQEELTEYYRLIAQLESQLLEASDsILTLLRLVVWLQPPLLLLRVLSNLVEAVEK----QNLKGGALLSLLHE 147

                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
gi 24642191    529 HTLLQVPH-QKLATALLFISLKRYCIIIDGWWRRAALEDHLREFIVE 574
Cdd:pfam17681  148 ATSHGDPFvRELLSRLLQRVSRPYLEMLERWIYEGELDDPYNEFFVE 194
 
Name Accession Description Interval E-value
GCP5_NTD cd22572
N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, ...
 25-130 2.86e-09

N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, also called TUBGCP5, is a component of the gamma-tubulin ring complex (gamma-TuRC), which is necessary for microtubule nucleation at the centrosome. The model corresponds to the N-terminal domain (NTD) of GCP-5, which is involved in protein-protein interactions. GCP-5 binds Mozart1, a microprotein that regulates subcellular targeting and microtubule formation activity of gamma-TuRC at different cell cycle stages, through its NTD.


Pssm-ID: 439339  Cd Length: 102  Bit Score: 55.25  E-value: 2.86e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191   25 INENVRKLVRSLSEPGTSQKKLRQMEDTALKIIANQRRTwapTTTDMEVKRNLDDLAERFRAEGMASFGESIKDLAIRYL 104
Cdd:cd22572    1 LEELLEELITALTGFQEDDENFRLCLDFALSNLRYHRFL---STNSFEVERRLDGLVEKFRVHGQDDLADALRELLDELL 77

                         90       100
                 ....*....|....*....|....*.
gi 24642191  105 AMDENQQQfNPNPGWQMLELLFRIAD 130
Cdd:cd22572   78 KLPLFDHS-QSDWHPSILSLLLELSD 102
GCP_C_terminal pfam04130
Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components ...
 873-1082 1.57e-07

Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Structure-based sequence analysis revealed the existence of an exposed surface area conserved in all human GCPs and in GCP4 orthologs. This area is located in the C-terminal domain of GCP4, which was confirmed in vitro to bind directly to gamma-tubulin. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains.


Pssm-ID: 461187  Cd Length: 297  Bit Score: 54.17  E-value: 1.57e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    873 ILTPAHMQDYNSVWRLMLKVKWAVWKLENMQFLRRARFNPcapldliGLTIRRLEIVRFWLIFLINNLHAHIMEAVS--- 949
Cdd:pfam04130   95 VLTPEALTKYQRLFRFLLRLKRVEFVLSSLWRRRQMSGSR-------SVLWHRARLLRQEMIHFVSQLQYYVMFEVIeps 167

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    950 -RQFELRIG-ECKNVRELRIMHDEHLAWLKTHCMLTDEFKAFRVALDQIFhlvyvldmewttcaSCMHDHdALCLDVTIS 1027
Cdd:pfam04130  168 wREFEEKLQkAASDLDDLIEAHEDFLDRILKKCFLTSPQQPLLKLLEEIL--------------SLILDF-AEALDGLYL 232

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 24642191   1028 DDGIDDSETQRRSLEYLALNQVAEIELTYIRCHQMLADILNTLVHQNDHGFLSAL 1082
Cdd:pfam04130  233 SVSESARAEAEDELPELERERLRRLEKQFRKKVSLLLKVLRGLKSHPDESHLRQL 287
GCP_N_terminal pfam17681
Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components ...
 371-574 7.23e-07

Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Functional studies have shown that the N-terminal domain defines the functional identity of GCPs, suggesting that all GCPs are incorporated into the helix of gamma-tubulin small complexes (gTURCs) via lateral interactions between their N-terminal domains. Thereby, they define the direct neighbors and position the GCPs within the helical wall of gTuRC. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains. In addition to the conserved sequences, the N-terminal domains carry specific insertions of various sizes depending on the GCP, i.e. internal insertions or N-terminal extensions. These insertions may equally contribute to the function of individual GCPs as they have been implied in specific interactions with regulatory or structural proteins. For instance, GCP6 carries a large internal insertion phosphorylated by Plk4 and containing a domain of interaction with keratins, whereas the N-terminal extension of GCP3 interacts with the recruitment protein MOZART1.


Pssm-ID: 465456  Cd Length: 298  Bit Score: 52.29  E-value: 7.23e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    371 LREILFMFV-RPASccFFEFDKATRRITVRDNVSiCTVTASTMQnfLLFDVVPALEDMMELRHIIDTHTLHFHGvkttYT 449
Cdd:pfam17681    1 LRDLLFALQgISGS--YIRFDESDSRIVDDIRIP-GILPPSLRS--LLSRLLELGLLYRRLRKFVESSSSFEYG----LV 71

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    450 VEFFAYGLRDLVQPICQLLIAYEDRVNKDPA-KNTLIRFTIEFREHFSQLRLLRELAEDVILdkgpAHIRSAYLLSRLYK 528
Cdd:pfam17681   72 LQALCAALQEELTEYYRLIAQLESQLLEASDsILTLLRLVVWLQPPLLLLRVLSNLVEAVEK----QNLKGGALLSLLHE 147

                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
gi 24642191    529 HTLLQVPH-QKLATALLFISLKRYCIIIDGWWRRAALEDHLREFIVE 574
Cdd:pfam17681  148 ATSHGDPFvRELLSRLLQRVSRPYLEMLERWIYEGELDDPYNEFFVE 194
 
Name Accession Description Interval E-value
GCP5_NTD cd22572
N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, ...
 25-130 2.86e-09

N-terminal domain of gamma-tubulin complex component 5 (GCP-5) and similar proteins; GCP-5, also called TUBGCP5, is a component of the gamma-tubulin ring complex (gamma-TuRC), which is necessary for microtubule nucleation at the centrosome. The model corresponds to the N-terminal domain (NTD) of GCP-5, which is involved in protein-protein interactions. GCP-5 binds Mozart1, a microprotein that regulates subcellular targeting and microtubule formation activity of gamma-TuRC at different cell cycle stages, through its NTD.


Pssm-ID: 439339  Cd Length: 102  Bit Score: 55.25  E-value: 2.86e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191   25 INENVRKLVRSLSEPGTSQKKLRQMEDTALKIIANQRRTwapTTTDMEVKRNLDDLAERFRAEGMASFGESIKDLAIRYL 104
Cdd:cd22572    1 LEELLEELITALTGFQEDDENFRLCLDFALSNLRYHRFL---STNSFEVERRLDGLVEKFRVHGQDDLADALRELLDELL 77

                         90       100
                 ....*....|....*....|....*.
gi 24642191  105 AMDENQQQfNPNPGWQMLELLFRIAD 130
Cdd:cd22572   78 KLPLFDHS-QSDWHPSILSLLLELSD 102
GCP_C_terminal pfam04130
Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components ...
 873-1082 1.57e-07

Gamma tubulin complex component C-terminal; This is the C-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Structure-based sequence analysis revealed the existence of an exposed surface area conserved in all human GCPs and in GCP4 orthologs. This area is located in the C-terminal domain of GCP4, which was confirmed in vitro to bind directly to gamma-tubulin. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains.


Pssm-ID: 461187  Cd Length: 297  Bit Score: 54.17  E-value: 1.57e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    873 ILTPAHMQDYNSVWRLMLKVKWAVWKLENMQFLRRARFNPcapldliGLTIRRLEIVRFWLIFLINNLHAHIMEAVS--- 949
Cdd:pfam04130   95 VLTPEALTKYQRLFRFLLRLKRVEFVLSSLWRRRQMSGSR-------SVLWHRARLLRQEMIHFVSQLQYYVMFEVIeps 167

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    950 -RQFELRIG-ECKNVRELRIMHDEHLAWLKTHCMLTDEFKAFRVALDQIFhlvyvldmewttcaSCMHDHdALCLDVTIS 1027
Cdd:pfam04130  168 wREFEEKLQkAASDLDDLIEAHEDFLDRILKKCFLTSPQQPLLKLLEEIL--------------SLILDF-AEALDGLYL 232

                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 24642191   1028 DDGIDDSETQRRSLEYLALNQVAEIELTYIRCHQMLADILNTLVHQNDHGFLSAL 1082
Cdd:pfam04130  233 SVSESARAEAEDELPELERERLRRLEKQFRKKVSLLLKVLRGLKSHPDESHLRQL 287
GCP_N_terminal pfam17681
Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components ...
 371-574 7.23e-07

Gamma tubulin complex component N-terminal; This is the N-terminal domain found in components of the gamma-tubulin complex proteins (GCPs). Family members include spindle pole body (SBP) components such as Spc97 and Spc98 which function as the microtubule-organizing center in yeast. Furthermore, family members such as human GCP4 (Gamma-tubulin complex component 4) have been structurally elucidated. Functional studies have shown that the N-terminal domain defines the functional identity of GCPs, suggesting that all GCPs are incorporated into the helix of gamma-tubulin small complexes (gTURCs) via lateral interactions between their N-terminal domains. Thereby, they define the direct neighbors and position the GCPs within the helical wall of gTuRC. Sequence alignment of human GCPs based on the GCP4 structure helped delineate conserved regions in the N- and C-terminal domains. In addition to the conserved sequences, the N-terminal domains carry specific insertions of various sizes depending on the GCP, i.e. internal insertions or N-terminal extensions. These insertions may equally contribute to the function of individual GCPs as they have been implied in specific interactions with regulatory or structural proteins. For instance, GCP6 carries a large internal insertion phosphorylated by Plk4 and containing a domain of interaction with keratins, whereas the N-terminal extension of GCP3 interacts with the recruitment protein MOZART1.


Pssm-ID: 465456  Cd Length: 298  Bit Score: 52.29  E-value: 7.23e-07
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    371 LREILFMFV-RPASccFFEFDKATRRITVRDNVSiCTVTASTMQnfLLFDVVPALEDMMELRHIIDTHTLHFHGvkttYT 449
Cdd:pfam17681    1 LRDLLFALQgISGS--YIRFDESDSRIVDDIRIP-GILPPSLRS--LLSRLLELGLLYRRLRKFVESSSSFEYG----LV 71

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24642191    450 VEFFAYGLRDLVQPICQLLIAYEDRVNKDPA-KNTLIRFTIEFREHFSQLRLLRELAEDVILdkgpAHIRSAYLLSRLYK 528
Cdd:pfam17681   72 LQALCAALQEELTEYYRLIAQLESQLLEASDsILTLLRLVVWLQPPLLLLRVLSNLVEAVEK----QNLKGGALLSLLHE 147

                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
gi 24642191    529 HTLLQVPH-QKLATALLFISLKRYCIIIDGWWRRAALEDHLREFIVE 574
Cdd:pfam17681  148 ATSHGDPFvRELLSRLLQRVSRPYLEMLERWIYEGELDDPYNEFFVE 194
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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