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Conserved domains on  [gi|24641578|ref|NP_572818|]
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uncharacterized protein Dmel_CG2540 [Drosophila melanogaster]

Protein Classification

glutathione-specific gamma-glutamylcyclotransferase( domain architecture ID 10520616)

glutathione-specific gamma-glutamylcyclotransferase (gamma-glu-GCG or gamma-GCG) catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide; acts specifically on glutathione, but not on other gamma-glutamyl peptides

CATH:  3.10.490.10
EC:  4.3.2.7
Gene Ontology:  GO:0016746
PubMed:  18515354|23070364
SCOP:  4002485

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 74-251 5.51e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


:

Pssm-ID: 398428  Cd Length: 176  Bit Score: 286.21  E-value: 5.51e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578    74 VWIFGYGSLVWKTDFPYIDRRRGFVWGFKRRFYQHSIDHRGIPERPGRVVTLLPGDpaQDRVYGVAYRIAASQKGAVLDH 153
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDE--EGRTWGVAYRIPAETAEEVLEY 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578   154 LDYREK-NGYERCSLEFHEYPTDGAEPIQVIMYVATQANDSYAGDVwQVPCIARQIFSSAGPSGPNREYLFNLAAAMDQL 232
Cdd:pfam04752  79 LDVREQiNGYTTHEVPFYPDQDTDGRPVNALVYVATPKNPQYLGDD-PLEQIAKQIATARGPSGPNAEYLFELAQALRKL 157

                         170
                  ....*....|....*....
gi 24641578   233 FPGAVDEHLEELVACVKRY 251
Cdd:pfam04752 158 GPGVQDDHLFELEKLVKKL 176
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 74-251 5.51e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 286.21  E-value: 5.51e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578    74 VWIFGYGSLVWKTDFPYIDRRRGFVWGFKRRFYQHSIDHRGIPERPGRVVTLLPGDpaQDRVYGVAYRIAASQKGAVLDH 153
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDE--EGRTWGVAYRIPAETAEEVLEY 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578   154 LDYREK-NGYERCSLEFHEYPTDGAEPIQVIMYVATQANDSYAGDVwQVPCIARQIFSSAGPSGPNREYLFNLAAAMDQL 232
Cdd:pfam04752  79 LDVREQiNGYTTHEVPFYPDQDTDGRPVNALVYVATPKNPQYLGDD-PLEQIAKQIATARGPSGPNAEYLFELAQALRKL 157

                         170
                  ....*....|....*....
gi 24641578   233 FPGAVDEHLEELVACVKRY 251
Cdd:pfam04752 158 GPGVQDDHLFELEKLVKKL 176
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 62-256 1.08e-56

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 181.56  E-value: 1.08e-56
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578  62 NQLEPPAATDADVWIFGYGSLVWKTDFPYIDRRRGFVWGFKRRFYQHSIDHRGIPERPGRVVTLLPGdpaqDRVYGVAYR 141
Cdd:COG3703   4 NRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRG----GSCRGVAFR 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578 142 IAASQKGAVLDHLDYREKN--GYERCSLEFHeypTDGAEPIQVIMYVATQANDSYAGDVwQVPCIARQIFSSAGPSGPNR 219
Cdd:COG3703  80 VPPEDAEAVLAYLWRREMVtgVYLPRWLPVR---LADGRRVEALAFVADRDHPQYAGGL-SLEEQARIIAQAVGPSGSNA 155

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 24641578 220 EYLFNLAAAMDQLfpGAVDEHLEELVACVKRYIVEDE 256
Cdd:COG3703 156 DYLLNTAEHLREL--GIRDPHLEALARLVRALLAAAT 190
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 76-187 3.74e-14

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 66.98  E-value: 3.74e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578  76 IFGYGSLVWKTDF-----PYIDRRRGFVWGFKRRFYQHSiDHRGIPERPGRvvtllpgdpaqdRVYGVAYRIAAsqkgAV 150
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-GYPGLVPGPGA------------RVWGELYEVDP----ED 63

                        90       100       110
                ....*....|....*....|....*....|....*....
gi 24641578 151 LDHLDYREK--NGYERCSLEFHeypTDGAEPIQVIMYVA 187
Cdd:cd06661  64 LARLDAFEGvpGGYRREEVEVE---LEDGEGVEAWVYVA 99
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 74-251 5.51e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 286.21  E-value: 5.51e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578    74 VWIFGYGSLVWKTDFPYIDRRRGFVWGFKRRFYQHSIDHRGIPERPGRVVTLLPGDpaQDRVYGVAYRIAASQKGAVLDH 153
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDE--EGRTWGVAYRIPAETAEEVLEY 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578   154 LDYREK-NGYERCSLEFHEYPTDGAEPIQVIMYVATQANDSYAGDVwQVPCIARQIFSSAGPSGPNREYLFNLAAAMDQL 232
Cdd:pfam04752  79 LDVREQiNGYTTHEVPFYPDQDTDGRPVNALVYVATPKNPQYLGDD-PLEQIAKQIATARGPSGPNAEYLFELAQALRKL 157

                         170
                  ....*....|....*....
gi 24641578   233 FPGAVDEHLEELVACVKRY 251
Cdd:pfam04752 158 GPGVQDDHLFELEKLVKKL 176
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 62-256 1.08e-56

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 181.56  E-value: 1.08e-56
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578  62 NQLEPPAATDADVWIFGYGSLVWKTDFPYIDRRRGFVWGFKRRFYQHSIDHRGIPERPGRVVTLLPGdpaqDRVYGVAYR 141
Cdd:COG3703   4 NRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRG----GSCRGVAFR 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578 142 IAASQKGAVLDHLDYREKN--GYERCSLEFHeypTDGAEPIQVIMYVATQANDSYAGDVwQVPCIARQIFSSAGPSGPNR 219
Cdd:COG3703  80 VPPEDAEAVLAYLWRREMVtgVYLPRWLPVR---LADGRRVEALAFVADRDHPQYAGGL-SLEEQARIIAQAVGPSGSNA 155

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 24641578 220 EYLFNLAAAMDQLfpGAVDEHLEELVACVKRYIVEDE 256
Cdd:COG3703 156 DYLLNTAEHLREL--GIRDPHLEALARLVRALLAAAT 190
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 76-187 3.74e-14

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 66.98  E-value: 3.74e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24641578  76 IFGYGSLVWKTDF-----PYIDRRRGFVWGFKRRFYQHSiDHRGIPERPGRvvtllpgdpaqdRVYGVAYRIAAsqkgAV 150
Cdd:cd06661   1 LFVYGTLMDGEVLharlgRALFLGPATLKGYRLVFGGGS-GYPGLVPGPGA------------RVWGELYEVDP----ED 63

                        90       100       110
                ....*....|....*....|....*....|....*....
gi 24641578 151 LDHLDYREK--NGYERCSLEFHeypTDGAEPIQVIMYVA 187
Cdd:cd06661  64 LARLDAFEGvpGGYRREEVEVE---LEDGEGVEAWVYVA 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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