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Conserved domains on  [gi|18408605|ref|NP_566900|]
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kinetochore protein [Arabidopsis thaliana]

Protein Classification

kinetochore Spc25 family protein( domain architecture ID 19231304)

kinetochore Spc25 family protein is involved in kinetochore-microtubule interactions and spindle checkpoint activity

Gene Ontology:  GO:0000776|GO:0031262|GO:0007059|GO:0007052
PubMed:  38605119

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 148-232 1.47e-25

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


:

Pssm-ID: 467646  Cd Length: 87  Bit Score: 97.27  E-value: 1.47e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18408605 148 KADIHEAISWYNHALGFHVE--AGHGVKFTFTNIDAKRPTREFSFTVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLF 225
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEktKDDRLKFVFTNIDPKDPEREFSFVLDVSEDDYKVVECDPPLEDLDELLEELNETRDFS 80


                ....*..
gi 18408605 226 RFVRLMR 232
Cdd:cd23784  81 AFLKRMR 87
 
Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 148-232 1.47e-25

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


Pssm-ID: 467646  Cd Length: 87  Bit Score: 97.27  E-value: 1.47e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18408605 148 KADIHEAISWYNHALGFHVE--AGHGVKFTFTNIDAKRPTREFSFTVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLF 225
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEktKDDRLKFVFTNIDPKDPEREFSFVLDVSEDDYKVVECDPPLEDLDELLEELNETRDFS 80


                ....*..
gi 18408605 226 RFVRLMR 232
Cdd:cd23784  81 AFLKRMR 87
Spindle_Spc25 pfam08234
Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It ...
 169-235 1.16e-22

Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It contains Spc25, which is a conserved eukaryotic kinetochore protein involved in cell division. In fungi the Spc25 protein is a subunit of the Nuf2-Ndc80 complex, and in vertebrates it forms part of the Ndc80 complex.


Pssm-ID: 462402  Cd Length: 71  Bit Score: 89.17  E-value: 1.16e-22
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 18408605   169 GHGVKFTFTNIDAKRPTREFSFTVHYGNDIYTLLDSDLQLD--YINEMVQELNKTNDLFRFVRLMREQF 235
Cdd:pfam08234   3 GDKLKFVFTNIDPKDPEREFSFTLDVSEDTYEVLECDPKLEdeDLEELLDELNETNDLFKFLKRMRKAF 71
 
Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 148-232 1.47e-25

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


Pssm-ID: 467646  Cd Length: 87  Bit Score: 97.27  E-value: 1.47e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18408605 148 KADIHEAISWYNHALGFHVE--AGHGVKFTFTNIDAKRPTREFSFTVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLF 225
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEktKDDRLKFVFTNIDPKDPEREFSFVLDVSEDDYKVVECDPPLEDLDELLEELNETRDFS 80


                ....*..
gi 18408605 226 RFVRLMR 232
Cdd:cd23784  81 AFLKRMR 87
Spindle_Spc25 pfam08234
Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It ...
 169-235 1.16e-22

Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It contains Spc25, which is a conserved eukaryotic kinetochore protein involved in cell division. In fungi the Spc25 protein is a subunit of the Nuf2-Ndc80 complex, and in vertebrates it forms part of the Ndc80 complex.


Pssm-ID: 462402  Cd Length: 71  Bit Score: 89.17  E-value: 1.16e-22
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 18408605   169 GHGVKFTFTNIDAKRPTREFSFTVHYGNDIYTLLDSDLQLD--YINEMVQELNKTNDLFRFVRLMREQF 235
Cdd:pfam08234   3 GDKLKFVFTNIDPKDPEREFSFTLDVSEDTYEVLECDPKLEdeDLEELLDELNETNDLFKFLKRMRKAF 71
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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