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Conserved domains on  [gi|18399730|ref|NP_566435|]
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ALA-interacting subunit 1 [Arabidopsis thaliana]

Protein Classification

CDC50/LEM3 family protein( domain architecture ID 10506127)

CDC50/LEM3 (ligand-effect modulator 3) family protein is a membrane protein, such as cell cycle control protein 50 (CDC50) and plant ALA-interacting subunits

Gene Ontology:  GO:0016020

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
70-334 1.22e-135

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


:

Pssm-ID: 460906  Cd Length: 292  Bit Score: 387.73  E-value: 1.22e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730    70 LFASQDVVEIVDRYDSACI--PLSDRANKVAYIQGT-GNKSCTRTLIVPKRMKQPIYVYYQLENFYQNHRRYVKSRSDSQ 146
Cdd:pfam03381   2 LYASNNVQEIVIDYTDDCAtlAPTDNFNPIDYIQNSnETKTCTIQFTIPKDMKGPVYLYYKLTNFYQNHRRYVKSRDDDQ 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   147 LR--SVKDENQIDACKPEDDFG----GQPIVPCGLIAWSLFNDTYVLSRNNQGL---------TVNKKGIAWKSDKEHKF 211
Cdd:pfam03381  82 LKgkAVSALLSGSSCKPLRYNGarddGKPIYPCGLIANSMFNDTFTLYNNSSPLllnvgtstyPLTKKGIAWPSDKEHKF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   212 GKN--------VFPKNFQKGNLTGGaSLDPNKPLSDQEDLIVWMRTAALPTFRKLYGKIE--SDLEKGeNIQVTLQNNYN 281
Cdd:pfam03381 162 KNPtkynasdiVPPPNWQKRYPNGY-TEDNIPNLSENEDFIVWMRTAALPTFRKLYRRINdtDGLPAG-TYTIDIGYNYP 239
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 18399730   282 TYSFSGKKKLVLSTTSWLGGKNDFLGIAYLTVGGICFVLALAFTVMYLVKPRR 334
Cdd:pfam03381 240 VTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKPRK 292
 
Name Accession Description Interval E-value
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
70-334 1.22e-135

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 387.73  E-value: 1.22e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730    70 LFASQDVVEIVDRYDSACI--PLSDRANKVAYIQGT-GNKSCTRTLIVPKRMKQPIYVYYQLENFYQNHRRYVKSRSDSQ 146
Cdd:pfam03381   2 LYASNNVQEIVIDYTDDCAtlAPTDNFNPIDYIQNSnETKTCTIQFTIPKDMKGPVYLYYKLTNFYQNHRRYVKSRDDDQ 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   147 LR--SVKDENQIDACKPEDDFG----GQPIVPCGLIAWSLFNDTYVLSRNNQGL---------TVNKKGIAWKSDKEHKF 211
Cdd:pfam03381  82 LKgkAVSALLSGSSCKPLRYNGarddGKPIYPCGLIANSMFNDTFTLYNNSSPLllnvgtstyPLTKKGIAWPSDKEHKF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   212 GKN--------VFPKNFQKGNLTGGaSLDPNKPLSDQEDLIVWMRTAALPTFRKLYGKIE--SDLEKGeNIQVTLQNNYN 281
Cdd:pfam03381 162 KNPtkynasdiVPPPNWQKRYPNGY-TEDNIPNLSENEDFIVWMRTAALPTFRKLYRRINdtDGLPAG-TYTIDIGYNYP 239
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 18399730   282 TYSFSGKKKLVLSTTSWLGGKNDFLGIAYLTVGGICFVLALAFTVMYLVKPRR 334
Cdd:pfam03381 240 VTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKPRK 292
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
26-345 7.80e-90

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 274.06  E-value: 7.80e-90
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730  26 SKRPKYSKFTQQELPACKPILTPGWVISTFLIISVIFIPLGVISLFASQDVVEIVDRYdSACIPLSDRA----------- 94
Cdd:COG5035  20 SRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDY-TDCMTLASDEfsdipseyiqf 98
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730  95 ---NKVAY--------IQGTGNKSCTRTLIVPKRMKQPIYVYYQLENFYQNHRRYVKSRSDSQLRSV--KDENQIDACKP 161
Cdd:COG5035  99 hfkKKVNVlpqwrfstDEEDDFQKCQIRFTVPSDMKKPVFIYYRLTNFYQNHRRYVKSFDEDQLRGEalKSDDLKSNCKP 178
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730 162 EDDFGGQPIVPCGLIAWSLFNDTYVLSRNNQGLTVN----KKGIAWKSDKeHKFGKN-------VFPKNFQKgNLTGGAS 230
Cdd:COG5035 179 LSYNEDKIIYPCGLIANSMFNDTFSSLLTGIEDTSNynltTKGIAWESDR-HRYKKTkynasdiVPPPNWAK-MYPDGYT 256
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730 231 lDPNKP-LSDQEDLIVWMRTAALPTFRKLYGKIESD-LEKGEnIQVTLQNNYNTYSFSGKKKLVLSTTSWLGGKNDFLGI 308
Cdd:COG5035 257 -DDNIPdLSTWEEFQNWMRTAAFPKFSKLAMRNVNDvLPPGT-YQLNITLNFPVLEFNGTKSIVLTTNSVIGGKNYFLGI 334
                       330       340       350
                ....*....|....*....|....*....|....*..
gi 18399730 309 AYLTVGGICFVLALAFTVMYLVKPRRLGDPTYLSWNR 345
Cdd:COG5035 335 VYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNWNM 371
 
Name Accession Description Interval E-value
CDC50 pfam03381
LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been ...
70-334 1.22e-135

LEM3 (ligand-effect modulator 3) family / CDC50 family; Members of this family have been predicted to contain transmembrane helices. The family member LEM3 is a ligand-effect modulator, mutation of which increases glucocorticoid receptor activity in response to dexamethasone and also confers increased activity on other intracellular receptors including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells. The products of genes YNR048w, YNL323w and YCR094w (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39. CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the general RNA polymerase II machinery or by altering chromatin structure. One function of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation causes arrest in G1 by activation of the pathway. It may be that the cold-sensitive arrest in G1 noticed in CDC50 mutants may be due to inactivation of CDC39. The effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via CDC39.


Pssm-ID: 460906  Cd Length: 292  Bit Score: 387.73  E-value: 1.22e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730    70 LFASQDVVEIVDRYDSACI--PLSDRANKVAYIQGT-GNKSCTRTLIVPKRMKQPIYVYYQLENFYQNHRRYVKSRSDSQ 146
Cdd:pfam03381   2 LYASNNVQEIVIDYTDDCAtlAPTDNFNPIDYIQNSnETKTCTIQFTIPKDMKGPVYLYYKLTNFYQNHRRYVKSRDDDQ 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   147 LR--SVKDENQIDACKPEDDFG----GQPIVPCGLIAWSLFNDTYVLSRNNQGL---------TVNKKGIAWKSDKEHKF 211
Cdd:pfam03381  82 LKgkAVSALLSGSSCKPLRYNGarddGKPIYPCGLIANSMFNDTFTLYNNSSPLllnvgtstyPLTKKGIAWPSDKEHKF 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730   212 GKN--------VFPKNFQKGNLTGGaSLDPNKPLSDQEDLIVWMRTAALPTFRKLYGKIE--SDLEKGeNIQVTLQNNYN 281
Cdd:pfam03381 162 KNPtkynasdiVPPPNWQKRYPNGY-TEDNIPNLSENEDFIVWMRTAALPTFRKLYRRINdtDGLPAG-TYTIDIGYNYP 239
                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 18399730   282 TYSFSGKKKLVLSTTSWLGGKNDFLGIAYLTVGGICFVLALAFTVMYLVKPRR 334
Cdd:pfam03381 240 VTSFGGTKSIVLSTTSWIGGKNPFLGIAYLVVGGICLLLGIVFLIIHLIKPRK 292
CDC50 COG5035
Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal ...
26-345 7.80e-90

Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms];


Pssm-ID: 227368  Cd Length: 372  Bit Score: 274.06  E-value: 7.80e-90
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730  26 SKRPKYSKFTQQELPACKPILTPGWVISTFLIISVIFIPLGVISLFASQDVVEIVDRYdSACIPLSDRA----------- 94
Cdd:COG5035  20 SRRPPNTAFRQQRLKAWQPILSPRTVLPLLFLIGIVFAPLGGGLLVASSSVQELTIDY-TDCMTLASDEfsdipseyiqf 98
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730  95 ---NKVAY--------IQGTGNKSCTRTLIVPKRMKQPIYVYYQLENFYQNHRRYVKSRSDSQLRSV--KDENQIDACKP 161
Cdd:COG5035  99 hfkKKVNVlpqwrfstDEEDDFQKCQIRFTVPSDMKKPVFIYYRLTNFYQNHRRYVKSFDEDQLRGEalKSDDLKSNCKP 178
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730 162 EDDFGGQPIVPCGLIAWSLFNDTYVLSRNNQGLTVN----KKGIAWKSDKeHKFGKN-------VFPKNFQKgNLTGGAS 230
Cdd:COG5035 179 LSYNEDKIIYPCGLIANSMFNDTFSSLLTGIEDTSNynltTKGIAWESDR-HRYKKTkynasdiVPPPNWAK-MYPDGYT 256
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 18399730 231 lDPNKP-LSDQEDLIVWMRTAALPTFRKLYGKIESD-LEKGEnIQVTLQNNYNTYSFSGKKKLVLSTTSWLGGKNDFLGI 308
Cdd:COG5035 257 -DDNIPdLSTWEEFQNWMRTAAFPKFSKLAMRNVNDvLPPGT-YQLNITLNFPVLEFNGTKSIVLTTNSVIGGKNYFLGI 334
                       330       340       350
                ....*....|....*....|....*....|....*..
gi 18399730 309 AYLTVGGICFVLALAFTVMYLVKPRRLGDPTYLSWNR 345
Cdd:COG5035 335 VYLIVGGICALLGLIFLIKWLFKPRKMADHSYLNWNM 371
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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