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Conserved domains on  [gi|392887389|ref|NP_493161|]
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LOW QUALITY PROTEIN: Glycoside hydrolase family 25 protein [Caenorhabditis elegans]

Protein Classification

glycoside hydrolase family 25 domain-containing protein( domain architecture ID 2550)

glycoside hydrolase family 25 domain-containing protein is a peptidoglycan hydrolase (also called lysozyme) that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GH25_muramidase super family cl10448
Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan ...
21-221 7.78e-30

Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.


The actual alignment was detected with superfamily member cd06416:

Pssm-ID: 447900  Cd Length: 196  Bit Score: 109.72  E-value: 7.78e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389  21 HKAVDVPQDITQDEGHCLWKDNNDITFINTLTSSGLFDSTAPRVFKNLVNYGGDMWFYMKPYPSNGKSGSQQMENVYQGL 100
Cdd:cd06416    1 ILGVDISQPTSVSTFQCLKNNGYSFAIIRAYRSNGSFDPNSVTNIKNARAAGLSTDVYFFPCINCCGSAAGQVQTFLQYL 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389 101 KSAQIKFSHITVHI-GDPQLWSSGSDANT-ISKDLIQRADQHGISVQFFTSQSNWHSITNN--NRSFSSYKLWWAHTNGa 176
Cdd:cd06416   81 KANGIKYGTVWIDIeQNPCQWSSDVASNCqFLQELVSAAKALGLKVGIYSSQYDWSQIFGSsyTCNFSSLPLWYAHYDN- 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 392887389 177 gnggatSPNFEGFVSFGGWTSSSLvgKEYARQVQECGVTVNKYVY 221
Cdd:cd06416  160 ------NPNFSDFSPFGGWTKPTM--KQYSGTTTVCGVSVDLNVY 196
 
Name Accession Description Interval E-value
GH25_Lys1-like cd06416
Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a ...
21-221 7.78e-30

Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a several lysozyme genes upregulated upon infection by the Gram-negative bacterial pathogen Serratia marcescens. Lys-1 contains a glycosyl hydrolase family 25 (GH25) catalytic domain. This family also includes Lys-5 from Caenorhabditis elegans.


Pssm-ID: 119378  Cd Length: 196  Bit Score: 109.72  E-value: 7.78e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389  21 HKAVDVPQDITQDEGHCLWKDNNDITFINTLTSSGLFDSTAPRVFKNLVNYGGDMWFYMKPYPSNGKSGSQQMENVYQGL 100
Cdd:cd06416    1 ILGVDISQPTSVSTFQCLKNNGYSFAIIRAYRSNGSFDPNSVTNIKNARAAGLSTDVYFFPCINCCGSAAGQVQTFLQYL 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389 101 KSAQIKFSHITVHI-GDPQLWSSGSDANT-ISKDLIQRADQHGISVQFFTSQSNWHSITNN--NRSFSSYKLWWAHTNGa 176
Cdd:cd06416   81 KANGIKYGTVWIDIeQNPCQWSSDVASNCqFLQELVSAAKALGLKVGIYSSQYDWSQIFGSsyTCNFSSLPLWYAHYDN- 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 392887389 177 gnggatSPNFEGFVSFGGWTSSSLvgKEYARQVQECGVTVNKYVY 221
Cdd:cd06416  160 ------NPNFSDFSPFGGWTKPTM--KQYSGTTTVCGVSVDLNVY 196
 
Name Accession Description Interval E-value
GH25_Lys1-like cd06416
Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a ...
21-221 7.78e-30

Lys-1 is a lysozyme encoded by the Caenorhabditis elegans lys-1 gene. This gene is one of a several lysozyme genes upregulated upon infection by the Gram-negative bacterial pathogen Serratia marcescens. Lys-1 contains a glycosyl hydrolase family 25 (GH25) catalytic domain. This family also includes Lys-5 from Caenorhabditis elegans.


Pssm-ID: 119378  Cd Length: 196  Bit Score: 109.72  E-value: 7.78e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389  21 HKAVDVPQDITQDEGHCLWKDNNDITFINTLTSSGLFDSTAPRVFKNLVNYGGDMWFYMKPYPSNGKSGSQQMENVYQGL 100
Cdd:cd06416    1 ILGVDISQPTSVSTFQCLKNNGYSFAIIRAYRSNGSFDPNSVTNIKNARAAGLSTDVYFFPCINCCGSAAGQVQTFLQYL 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392887389 101 KSAQIKFSHITVHI-GDPQLWSSGSDANT-ISKDLIQRADQHGISVQFFTSQSNWHSITNN--NRSFSSYKLWWAHTNGa 176
Cdd:cd06416   81 KANGIKYGTVWIDIeQNPCQWSSDVASNCqFLQELVSAAKALGLKVGIYSSQYDWSQIFGSsyTCNFSSLPLWYAHYDN- 159
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 392887389 177 gnggatSPNFEGFVSFGGWTSSSLvgKEYARQVQECGVTVNKYVY 221
Cdd:cd06416  160 ------NPNFSDFSPFGGWTKPTM--KQYSGTTTVCGVSVDLNVY 196
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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