FHA domain; The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799   188 YLIGRDHKiADIPVDHPSCSKQHAVLQFRSMPftrddgtkarriMPYIIDLGSGNGTFLNEKKIEPQRYiELQEKDMLKF 267
Cdd:pfam00498   1 VTIGRSPD-CDIVLDDPSVSRRHAEIRYDGGG------------RFYLEDLGSTNGTFVNGQRLGPEPV-RLKDGDVIRL 66

Forkhead associated domain; Found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain.

                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 17505799    188 YLIGRDHKIADIPVDHPSCSKQHAVLqfrsmpfTRDDGTKArrimpYIIDLGSGNGTFLNEKKI 251
Cdd:smart00240   1 VTIGRSSEDCDIQLDGPSISRRHAVI-------VYDGGGRF-----YLIDLGSTNGTFVNGKRI 52

forkhead associated (FHA) domain found in Smad nuclear-interacting protein 1 (SNIP1), FHA domain-containing protein DDL, and similar proteins; SNIP1 is an FHA domain-containing protein required for pre-mRNA splicing as a component of the spliceosome. It inhibits NF-kappaB signaling by competing for its binding to the C/H1 domain of CBP/p300 transcriptional co-activators. It is involved in microRNA (miRNA) biogenesis. SNIP1 is a regulator of the cell cycle and cyclin D1 expression and may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. This family also includes Arabidopsis thaliana FHA domain-containing protein DDL and similar proteins. DDL, also called protein DAWDLE, is involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. It may facilitate DCL1 to access or recognize primary miRNAs. DDL binds RNA non-specifically. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 166 WRLYPFKGEESLQVLYIHRQSAYLIGRDHKIADIPVDHPSCSKQHAVLQFRSMPfTRDDGTKARRIMPYIIDLGSGNGTF 245
Cdd:cd22676   1 WRLYVFKGGEQLETIDIHRQSAYLIGRDRRVADIPLDHPSCSKQHAVIQFREVE-KRNEGDVIENIRPYIIDLGSTNGTF 79

                        90       100       110
                ....*....|....*....|....*....|..
gi 17505799 246 LNEKKIEPQRYIELQEKDMLKFGFSTREYVVM 277
Cdd:cd22676  80 LNGEKIEPRRYYELREKDVLKFGLSTREYVLL 111

forkhead associated (FHA) domain found in kanadaptin and similar proteins; Kanadaptin, also called human lung cancer oncogene 3 protein (HLC-3), kidney anion exchanger adapter protein, or solute carrier family 4 anion exchanger member 1 adapter protein (SLC4A1AP), is a nuclear protein widely expressed in mammalian tissues. It was originally isolated as a kidney Cl-/HCO3- anion exchanger 1 (kAE1)-binding protein. It is a highly mobile nucleocytoplasmic shuttling and multilocalizing protein. Its role in mammalian cells remains unclear. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 185 QSAYLIGRdHKIADIPVDHPSCSKQHAVLQFRSMPFTRDDGTkarrimpYIIDLGSGNGTFLNEKKIEPQRYIELQEKDM 264
Cdd:cd22677  21 KSFYVFGR-LPGCDVVLEHPSISRYHAVLQYRGDADDHDGGF-------YLYDLGSTHGTFLNKQRIPPKQYYRLRVGHV 92

                        90
                ....*....|.
gi 17505799 265 LKFGFSTREYV 275
Cdd:cd22677  93 LKFGGSTRLYI 103

forkhead associated (FHA) domain found in Saccharomyces cerevisiae pre-mRNA leakage protein 1 (PML1) and similar proteins; PML1 is an FHA domain-containing protein required for efficient splicing and pre-mRNA nuclear retention. It is a component of the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3, and PML1. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 185 QSAYLIGRDHK------IADIPVDHPSCSKQHAVLQFRSmpfTRDdgtkarRIMPYIIDLGSGNGTFLNEKKIEPQRYIE 258
Cdd:cd22681  39 PSCYLIGREKGesteivVADIGIPEETCSKQHCVIQFRN---VKG------ILKPYIMDLDSSNGTCLNDNVIPSSRYVE 109

                        90       100
                ....*....|....*....|
gi 17505799 259 LQEKDMLKFGFSTREYVVMK 278
Cdd:cd22681 110 LRSGDVITFSKSNDYELVFM 129

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 167 RLYPFKGEESLQVLYIHRQSaYLIGRDHKiADIPVDHPSCSKQHAVLQFrsmpftrDDGTkarrimPYIIDLGSGNGTFL 246
Cdd:cd00060   1 RLIVLDGDGGGREFPLTKGV-VTIGRSPD-CDIVLDDPSVSRRHARIEV-------DGGG------VYLEDLGSTNGTFV 65

                        90       100
                ....*....|....*....|..
gi 17505799 247 NEKKIEPQryIELQEKDMLKFG 268
Cdd:cd00060  66 NGKRITPP--VPLQDGDVIRLG 85

Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 165 RWRLYPFKGEESLQVLYIHRQSaYLIGRDHKiADIPVDHPSCSKQHAVLqfrsmpfTRDDGTkarrimPYIIDLGSGNGT 244
Cdd:COG1716   1 MARLVVLEGPLAGRRFPLDGGP-LTIGRAPD-NDIVLDDPTVSRRHARI-------RRDGGG------WVLEDLGSTNGT 65

                        90       100       110
                ....*....|....*....|....*....|..
gi 17505799 245 FLNEKKIepQRYIELQEKDMLKFGFSTREYVV 276
Cdd:COG1716  66 FVNGQRV--TEPAPLRDGDVIRLGKTELRFRL 95

forkhead associated (FHA) domain found in Trypanosoma brucei Parvulin 42 (TbPar42) and similar proteins; TbPar42 is a nuclear protein that plays a key role in parasite cell proliferation. It exhibits an N-terminal forkhead associated (FHA)-domain and a peptidyl-prolyl-cis/trans-isomerase (PPIase) domain, both connected by a linker. Its PPIase domain adopts a parvulin fold and reflects structural elements of Pin1-type proteins but is catalytically inactive. Its FHA domain may be involved in the binding of phosphorylated target proteins. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 177 LQVLYIHRQSAYLIGRDhKIADIPVDHPSCSKQHAVLQFRSmpftrddGTKARRIMpyiiDLGSGNGTFLNEKKIEPQRY 256
Cdd:cd22675  20 LPPLGLDRFPFYLFGRS-PVCDYVLEHPSISSVHAVLVFHG-------EQKCFVLM----DLGSTNGVKLNGKRIEKGRP 87

                        90       100
                ....*....|....*....|..
gi 17505799 257 IELQEKDMLKFGFSTREYVVMK 278
Cdd:cd22675  88 LPLPVGSVIQFGFSARKYKVRK 109

forkhead associated (FHA) domain found in the centrosomal protein of 170 kDa protein (Cep170) family; The Cep170 family includes Cep170 and Cep170B. Cep170, also called Cep170A, KARP-1-binding protein, or KARP1-binding protein, is a protein that localizes to centrosomes as well as spindle microtubules and plays a role in microtubule organization and microtubule assembly. It is required for centriole subdistal appendage assembly. Cep170 is phosphorylated during M phase and interacts with Polo-like kinase 1 (Plk1). Cep170B, also called centrosomal protein 170B, plays a role in microtubule organization. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 183 HR--QSAYLIGRDHkiADIPVDHPSCSKQHAVLQFrsmpftrDDGTKARRIMpyiiDLGSGNGTFLNEKKIEPQRYIELQ 260
Cdd:cd22704  12 HRlpRSMLFVGRED--CDLILQSRSVDKQHAVITY-------DQIDNEFKIK----DLGSLNGTFVNDSRIPEQTYITLK 78

                        90       100
                ....*....|....*....|....
gi 17505799 261 EKDMLKFGFSTREYVVMKEREITE 284
Cdd:cd22704  79 LGDSIRFGYDTNVYRFEQLSLTTI 102

forkhead associated (FHA) domain found in Bdellovibrio bacteriovorus GGDEF domain protein DgcB and similar proteins; DgcB is a GGDEF enzyme that produces cyclic-di-GMP in response to an unknown stimulus. It appends the C-terminal GGDEF enzymatic domain with an N-terminal forkhead-associated (FHA) domain that acts as a consensus phosphopeptide sensor. The GGDEF and sensory FHA domains form an asymmetrical dimer.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 185 QSAYLIGRDHKiADIPVDHPSCSKQHAVLQFrsmpftrdDGTKARrimpyIIDLGSGNGTFLNEKKIEPQRYIELQEKDM 264
Cdd:cd22682  19 ESTIVIGRSVE-SQVQIDDDSVSRYHAKLAV--------NPSAVS-----IIDLGSTNGTIVNGKKIPKLASCDLQNGDQ 84

                ....
gi 17505799 265 LKFG 268
Cdd:cd22682  85 IKIG 88

forkhead associated (FHA) domain found in centrosomal protein of 170 kDa protein B (Cep170B) and similar proteins; Cep170B, also called centrosomal protein 170B, plays a role in microtubule organization. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 17505799 205 SCSKQHAVLQFRSmpfTRDDGtkarrimpYIIDLGSGNGTFLNEKKIEPQRYIELQEKDMLKFGFSTREYVVMK 278
Cdd:cd22725  38 SVDKQHAVINYDQ---DTDEH--------WVKDLGSLNGTFVNDVRIPDQKYITLKLNDVIRFGYDSNMYVLER 100

forkhead associated (FHA) domain found in Chlamydia trachomatis Ct664 protein and similar proteins; This subfamily corresponds to a group of uncharacterized FHA domain-containing proteins which show high sequence similarity with Chlamydia trachomatis Ct664 protein. Ct664 situates within the type III secretion system cluster that also encodes an STPK (CT673 in C. trachomatis), suggesting a role of CT664 in the chlamydial type III secretion system by mediating phosphorylation-dependent protein-protein interactions. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 186 SAYLIGRDHKIADIPVDHPSCSKQHAVLqfrsmpFTRDDGTKarrimpYIIDLGSGNGTFLNEKKIEPQRyiELQEKDML 265
Cdd:cd22696  21 KTYFIGKDPTVCDIVLQDPSISRQHARL------SIDQDNRV------FIEDLSSKNGVLVNGKPIEGKE--EISGSDVI 86

                ...
gi 17505799 266 KFG 268
Cdd:cd22696  87 SLG 89

forkhead associated (FHA) domain found in proliferation marker protein Ki-67 and similar proteins; Ki-67, also called antigen identified by monoclonal antibody Ki-67, antigen KI-67, or antigen Ki67, acts as a biological surfactant to disperse mitotic chromosomes. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Ki-67 binds DNA with a preference for supercoiled DNA and AT-rich DNA. It may also play a role in chromatin organization. Ki-67 contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 188 YLIGRDHKiADIPVDHPSCSKQHAVLQFrsmpftrDDGTKArrimpYIIDLGSGNGTFLNEKKIEPQryIELQEKDMLKF 267
Cdd:cd22673  23 CTFGRDLS-CDIRIQLPGVSREHCRIEV-------DENGKA-----YLENLSTTNPTLVNGKAIEKS--AELKDGDVITI 87

                .
gi 17505799 268 G 268
Cdd:cd22673  88 G 88

forkhead associated (FHA) domain found in angiogenic factor with G patch and FHA domains 1 (AGGF1) and similar proteins; AGGF1, also called angiogenic factor VG5Q, or G patch domain-containing protein 7 (GPATC7), or vasculogenesis gene on 5q protein, is an angiogenic factor involved in vascular development, angiogenesis, specification of hemangioblasts, and differentiation of veins. It promotes angiogenesis and the proliferation of endothelial cells. It inhibits inflammatory effect and preserve vascular integrity in non-nervous system diseases. Mutated AGGF1 causes susceptibility to Klippel-Trenaunay syndrome, a vascular disorder. Increased AGGF1 expression is associated with tumor angiogenesis. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 175 ESLQV--LYIHRQSAYLIGRD----HKIAdIPVDhpSCSKQHAvlQFRsmpFTRDDGTKarrimpYIIDLGSGNGTFLNE 248
Cdd:cd22686  13 PSLQVgsLFIVTATGATIGREkdhgHTIR-IPEL--GVSKFHA--EIY---YDDDEQSY------TIVDLGSQNGTYLNG 78

                        90       100
                ....*....|....*....|....*..
gi 17505799 249 KKIEPQR----YIELQEKDMLKFGFST 271
Cdd:cd22686  79 VRISQPKeksdPYPLTHGDELKIGETT 105

forkhead associated (FHA) domain found in transcription factor 19 (TCF-19) and similar proteins; TCF-19, also called transcription factor SC1, was identified as a putative trans-activating factor with expression beginning at the late G1-S boundary in dividing cells. It also functions as a novel islet factor necessary for proliferation and survival in the INS-1 beta cell line. It plays an important role in susceptibility to both Type 1 Diabetes Mellitus (T1DM) and Type 2 Diabetes Mellitus (T2DM); it has been suggested that it may positively impact beta cell mass under conditions of beta cell stress and increased insulin demand. TCF-19 contains an N-terminal fork head association domain (FHA), a proline rich region, and a C-terminal plant homeodomain (PHD) finger. The FHA domain may serve as a nuclear signaling domain or as a phosphoprotein binding domain. The proline rich region is a common characteristic of trans-activating factors. The PHD finger may allow TCF-19 to interact with chromatin via methylated histone H3.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 185 QSAYLIGRDHKIADIPVD---HPS-CSKQHAVLQFrsmpfTRDDGTKARrimpYIIDLGSGNGTFLNEKKIEPQRYIELQ 260
Cdd:cd22685  27 LCEYRIGRNPEVCDVFLCssqHPNlISREHAEIHA-----ERDGNGNWK----VLIEDRSTNGTYVNDVRLQDGQRRELS 97

                ....*...
gi 17505799 261 EKDMLKFG 268
Cdd:cd22685  98 DGDTITFG 105

forkhead associated (FHA) domain found in RING finger protein 8 (RNF8) and similar proteins; RNF8 is a telomere-associated E3 ubiquitin-protein ligase that plays an important role in DNA double-strand break (DSB) repair via histone ubiquitination. It is localized in the nucleus and interacts with class III E2s (UBE2E2, UbcH6, and UBE2E3), but not with other E2s (UbcH5, UbcH7, UbcH10, hCdc34, and hBendless). It recruits UBC13 for lysine 63-based self polyubiquitylation. Its deficiency causes neuronal pathology and cognitive decline, and its loss results in neuron degeneration. RNF8, together with RNF168, catalyzes a series of ubiquitylation events on substrates such as H2A and H2AX, with the H2AK13/15 ubiquitylation being particularly important for recruitment of repair factors p53-binding protein 1 (53BP1) or the RAP80-BRCA1 complex to sites of DSBs. Specially, RNF8 mediates the ubiquitination of gammaH2AX, and recruits 53BP1 and BRCA1 to DNA damage sites which promotes DNA damage response (DDR) and inhibits chromosomal instability. Moreover, RNF8 interacts with retinoid X receptor alpha (RXR alpha) and enhances its transcription-stimulating activity. It also regulates the rate of exit from mitosis and cytokinesis. RNF8 contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 190 IGR--DHKIADIPVDHPSCSKQHAVLQFRsmpftrDDGTKarrimpYIIDLGSGNGTFLNEKKIEPQRYIELQEKDMLKF 267
Cdd:cd22663  25 VGRglGVTYQLVSTCPLMISRNHCVLKKN------DEGQW------TIKDNKSLNGVWVNGERIEPLKPYPLNEGDLIQL 92

                .
gi 17505799 268 G 268
Cdd:cd22663  93 G 93

forkhead associated (FHA) domain found in Mycobacterium tuberculosis FHA domain-containing protein FhaB and similar proteins; FhaB, also called FtsZ-interacting protein A (FipA), is a putative virulence factor involved in regulating cell shape. It can interact with polyketide-associated protein PapA5, a putative membrane protein involved in the biosynthesis of virulence enhancing lipids. FhaB regulates growth and cell division. It is probably required for divisomal protein assembly under oxidative stress. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 186 SAYLIGR--DHkiaDIPVDHPSCSKQHAVLQFRSMPFtrddgtkarrimpYIIDLGSGNGTFLNEKKIEPQryIELQEKD 263
Cdd:cd22693  18 SGITIGRadDN---DLVLSDDFVSSRHARIYLQGSSW-------------YLEDLGSTNGTFVNGNRVTQP--VVVQPGD 79

                ....*
gi 17505799 264 MLKFG 268
Cdd:cd22693  80 TIRIG 84

forkhead associated (FHA) domain found in Myxococcus xanthus EspA and similar proteins; EspA is a histidine protein kinase with a fork head-associated (FHA) domain at the N-terminus and a receiver domain at the C-terminus. It functions as an inhibitor of sporulation during early fruiting body development while cells are aggregating into raised mounds. EspA is part of a two-component signal transduction system that regulates the timing of sporulation initiation. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17505799 185 QSAYLIGRDhKIADIPVDHPSCSKQHAVLQFRSMPFtrddgtkarrimpYIIDLGSGNGTFLNEKKIEPQryiELQEKDM 264
Cdd:cd22698  20 QDEFTIGRS-SNNDIRLNDHSVSRHHARIVRQGDKC-------------NLTDLGSTNGTFLNGIRVGTH---ELKHGDR 82

                ....*.
gi 17505799 265 LKFGFS 270
Cdd:cd22698  83 IQLGET 88