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Conserved domains on  [gi|93277088|ref|NP_443728|]
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mediator of RNA polymerase II transcription subunit 12-like protein isoform 2 [Homo sapiens]

Protein Classification

Med12-LCEWAV and Med12-PQL domain-containing protein( domain architecture ID 12100658)

protein containing domains Med12, Med12-LCEWAV, and Med12-PQL

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Med12-LCEWAV pfam12145
Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically ...
283-730 0e+00

Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. The function of this particular region of the Mediator subunit Med12 is not known, but there is a conserved sequence motif: LCEWAV, from which the name derives.


:

Pssm-ID: 463472  Cd Length: 471  Bit Score: 711.13  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    283 EFVQSAYLSRRLAYFCARRLSLLLSDS--PNLLAAHSPHMmiGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPL 360
Cdd:pfam12145    1 EFVQSELLSRRLAYFCARKLALLLNSSieSNGPNSHSPHS--SNNNSPALAGSPTTSPPGTNPVQSAFSDYLSCPHHRDI 78
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    361 VYGLSCMLQTVTLCCPSALVWNYsTNENKSANP--GSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAV 438
Cdd:pfam12145   79 VLGLSCIIQTITLECPTALVWNY-TGDGRSSSSlnGSPLDHLPCAPSVLPMPGRNSAFNQQVRAQLREAEEEIKERSQAV 157
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    439 EVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEV---APNDEAVVTLLCEWAV 515
Cdd:pfam12145  158 EVRWSFDKCQESTAGVTINRVLHTLEALDRHSFERSDSSNSLDTLYAKIFGPGSSKDNHEIldpVPQDEAVVKLLCEWAV 237
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    516 SCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVE 595
Cdd:pfam12145  238 SCKRSGEHRAMVVAKLLEKRQAEILAERCGESEVGDEKDSISSGLSAPSALPVFQNVLMKFLDTQAPVLDDNNSEQEKTE 317
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    596 FVNLVLLFCEFIRHDVFSHDAYMCTLISRGDL----SVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANT 671
Cdd:pfam12145  318 FTNLVLLFSELIRHDVFSHDAYMCTLISRGDLlstpSASPKPTPPSPAGEIADEHGRKDDHLVSGIDLKDPGLSEHMDFD 397
                          410       420       430       440       450       460       470
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088    672 SlgRRMSVNCEKLV---KREKPRELI--------------FPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILL 730
Cdd:pfam12145  398 D--SRIDDDLDKLLqhiKEEQQNSMDapdspkdaggvtggFGSVYDQSRHLLYTTHFPIPQDESSSHECNQRHVLL 471
Med12-PQL pfam12144
Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is ...
1802-2019 1.68e-75

Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. Both development and carcinogenesis are driven by signal transduction within the canonical Wnt/beta-catenin pathway through both programmed and unprogrammed changes in gene transcription. Beta-catenin physically and functionally targets this PQL (proline-, glutamine-, leucine-rich) region of the Med12 subunit of Mediator to activate transcription. The beta-catenin transactivation domain binds directly to isolated Med12 and intact Mediator both in vitro and in vivo, and Mediator is recruited to Wnt-responsive genes in a beta-catenin-dependent manner.


:

Pssm-ID: 463471 [Multi-domain]  Cd Length: 210  Bit Score: 250.14  E-value: 1.68e-75
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1802 QMMHHPQSTLWGYNlVGQPQQPGFFLQNQSLTPGGSRLDPagSFVPtNTKQaLSNMLQRRSGAM-MQPPSLHAITSQQQL 1880
Cdd:pfam12144    1 QLMHHPQSALWGRN-MYQPQQMGFYTQNQPLPPGGPRLDP--PYRP-VRKP-LSKMLPTRPNYPgMMPTMMGSIMSLDQQ 75
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1881 IQMKLLQQQQQQRLLRQA-----QTRPFQQGQPGDQaalfaaQARPSPQLPQYPGLQQaqtMPQGYTMYGTQMPLQQTsQ 1955
Cdd:pfam12144   76 LYKMKLYKQQPTMPQGQLlrqqlQARLLQQGMLGQQ------QARQMPPNPSYGSLQL---MPQGYTMYGSHMGLQQH-P 145
                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088   1956 QQAGSVVlSPSYNSRAYPAAH--SNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQ 2019
Cdd:pfam12144  146 SQAGGMV-PPSYSSQAYQGAHpgSNPALVDPLRQMQQRPSGYVHQQAPGYLHPLTNTQRFAHQPLQ 210
Med12 pfam09497
Transcription mediator complex subunit Med12; Med12 is a negative regulator of the ...
106-161 4.70e-17

Transcription mediator complex subunit Med12; Med12 is a negative regulator of the Gli3-dependent sonic hedgehog signalling pathway via its interaction with Gli3 within the RNA polymerase II transcriptional Mediator. A complex is formed between Med12, Med13, CDK8 and CycC which is responsible for suppression of transcription. This subunit forms part of the Kinase section of Mediator.


:

Pssm-ID: 462818  Cd Length: 63  Bit Score: 77.18  E-value: 4.70e-17
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 93277088    106 TARSQSAIHSWFSDLAG-NKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIK 161
Cdd:pfam09497    7 VTLTDTKREAWLRDLANpNVPLSKLSKKIPHGLKGKKLLEQLCSKNVPIPRAIWFIK 63
 
Name Accession Description Interval E-value
Med12-LCEWAV pfam12145
Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically ...
283-730 0e+00

Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. The function of this particular region of the Mediator subunit Med12 is not known, but there is a conserved sequence motif: LCEWAV, from which the name derives.


Pssm-ID: 463472  Cd Length: 471  Bit Score: 711.13  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    283 EFVQSAYLSRRLAYFCARRLSLLLSDS--PNLLAAHSPHMmiGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPL 360
Cdd:pfam12145    1 EFVQSELLSRRLAYFCARKLALLLNSSieSNGPNSHSPHS--SNNNSPALAGSPTTSPPGTNPVQSAFSDYLSCPHHRDI 78
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    361 VYGLSCMLQTVTLCCPSALVWNYsTNENKSANP--GSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAV 438
Cdd:pfam12145   79 VLGLSCIIQTITLECPTALVWNY-TGDGRSSSSlnGSPLDHLPCAPSVLPMPGRNSAFNQQVRAQLREAEEEIKERSQAV 157
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    439 EVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEV---APNDEAVVTLLCEWAV 515
Cdd:pfam12145  158 EVRWSFDKCQESTAGVTINRVLHTLEALDRHSFERSDSSNSLDTLYAKIFGPGSSKDNHEIldpVPQDEAVVKLLCEWAV 237
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    516 SCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVE 595
Cdd:pfam12145  238 SCKRSGEHRAMVVAKLLEKRQAEILAERCGESEVGDEKDSISSGLSAPSALPVFQNVLMKFLDTQAPVLDDNNSEQEKTE 317
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    596 FVNLVLLFCEFIRHDVFSHDAYMCTLISRGDL----SVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANT 671
Cdd:pfam12145  318 FTNLVLLFSELIRHDVFSHDAYMCTLISRGDLlstpSASPKPTPPSPAGEIADEHGRKDDHLVSGIDLKDPGLSEHMDFD 397
                          410       420       430       440       450       460       470
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088    672 SlgRRMSVNCEKLV---KREKPRELI--------------FPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILL 730
Cdd:pfam12145  398 D--SRIDDDLDKLLqhiKEEQQNSMDapdspkdaggvtggFGSVYDQSRHLLYTTHFPIPQDESSSHECNQRHVLL 471
Med12-PQL pfam12144
Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is ...
1802-2019 1.68e-75

Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. Both development and carcinogenesis are driven by signal transduction within the canonical Wnt/beta-catenin pathway through both programmed and unprogrammed changes in gene transcription. Beta-catenin physically and functionally targets this PQL (proline-, glutamine-, leucine-rich) region of the Med12 subunit of Mediator to activate transcription. The beta-catenin transactivation domain binds directly to isolated Med12 and intact Mediator both in vitro and in vivo, and Mediator is recruited to Wnt-responsive genes in a beta-catenin-dependent manner.


Pssm-ID: 463471 [Multi-domain]  Cd Length: 210  Bit Score: 250.14  E-value: 1.68e-75
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1802 QMMHHPQSTLWGYNlVGQPQQPGFFLQNQSLTPGGSRLDPagSFVPtNTKQaLSNMLQRRSGAM-MQPPSLHAITSQQQL 1880
Cdd:pfam12144    1 QLMHHPQSALWGRN-MYQPQQMGFYTQNQPLPPGGPRLDP--PYRP-VRKP-LSKMLPTRPNYPgMMPTMMGSIMSLDQQ 75
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1881 IQMKLLQQQQQQRLLRQA-----QTRPFQQGQPGDQaalfaaQARPSPQLPQYPGLQQaqtMPQGYTMYGTQMPLQQTsQ 1955
Cdd:pfam12144   76 LYKMKLYKQQPTMPQGQLlrqqlQARLLQQGMLGQQ------QARQMPPNPSYGSLQL---MPQGYTMYGSHMGLQQH-P 145
                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088   1956 QQAGSVVlSPSYNSRAYPAAH--SNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQ 2019
Cdd:pfam12144  146 SQAGGMV-PPSYSSQAYQGAHpgSNPALVDPLRQMQQRPSGYVHQQAPGYLHPLTNTQRFAHQPLQ 210
Med12 pfam09497
Transcription mediator complex subunit Med12; Med12 is a negative regulator of the ...
106-161 4.70e-17

Transcription mediator complex subunit Med12; Med12 is a negative regulator of the Gli3-dependent sonic hedgehog signalling pathway via its interaction with Gli3 within the RNA polymerase II transcriptional Mediator. A complex is formed between Med12, Med13, CDK8 and CycC which is responsible for suppression of transcription. This subunit forms part of the Kinase section of Mediator.


Pssm-ID: 462818  Cd Length: 63  Bit Score: 77.18  E-value: 4.70e-17
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 93277088    106 TARSQSAIHSWFSDLAG-NKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIK 161
Cdd:pfam09497    7 VTLTDTKREAWLRDLANpNVPLSKLSKKIPHGLKGKKLLEQLCSKNVPIPRAIWFIK 63
 
Name Accession Description Interval E-value
Med12-LCEWAV pfam12145
Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically ...
283-730 0e+00

Eukaryotic Mediator 12 subunit domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. The function of this particular region of the Mediator subunit Med12 is not known, but there is a conserved sequence motif: LCEWAV, from which the name derives.


Pssm-ID: 463472  Cd Length: 471  Bit Score: 711.13  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    283 EFVQSAYLSRRLAYFCARRLSLLLSDS--PNLLAAHSPHMmiGPNNSSIGAPSPGPPGPGMSPVQLAFSDFLSCAQHGPL 360
Cdd:pfam12145    1 EFVQSELLSRRLAYFCARKLALLLNSSieSNGPNSHSPHS--SNNNSPALAGSPTTSPPGTNPVQSAFSDYLSCPHHRDI 78
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    361 VYGLSCMLQTVTLCCPSALVWNYsTNENKSANP--GSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAV 438
Cdd:pfam12145   79 VLGLSCIIQTITLECPTALVWNY-TGDGRSSSSlnGSPLDHLPCAPSVLPMPGRNSAFNQQVRAQLREAEEEIKERSQAV 157
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    439 EVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEV---APNDEAVVTLLCEWAV 515
Cdd:pfam12145  158 EVRWSFDKCQESTAGVTINRVLHTLEALDRHSFERSDSSNSLDTLYAKIFGPGSSKDNHEIldpVPQDEAVVKLLCEWAV 237
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    516 SCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVE 595
Cdd:pfam12145  238 SCKRSGEHRAMVVAKLLEKRQAEILAERCGESEVGDEKDSISSGLSAPSALPVFQNVLMKFLDTQAPVLDDNNSEQEKTE 317
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088    596 FVNLVLLFCEFIRHDVFSHDAYMCTLISRGDL----SVTASTRPRSPVGENADEHYSKDHDVKMEIFSPMPGESCENANT 671
Cdd:pfam12145  318 FTNLVLLFSELIRHDVFSHDAYMCTLISRGDLlstpSASPKPTPPSPAGEIADEHGRKDDHLVSGIDLKDPGLSEHMDFD 397
                          410       420       430       440       450       460       470
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088    672 SlgRRMSVNCEKLV---KREKPRELI--------------FPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILL 730
Cdd:pfam12145  398 D--SRIDDDLDKLLqhiKEEQQNSMDapdspkdaggvtggFGSVYDQSRHLLYTTHFPIPQDESSSHECNQRHVLL 471
Med12-PQL pfam12144
Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is ...
1802-2019 1.68e-75

Eukaryotic Mediator 12 catenin-binding domain; This domain is found in eukaryotes, and is typically between 325 and 354 amino acids in length. Both development and carcinogenesis are driven by signal transduction within the canonical Wnt/beta-catenin pathway through both programmed and unprogrammed changes in gene transcription. Beta-catenin physically and functionally targets this PQL (proline-, glutamine-, leucine-rich) region of the Med12 subunit of Mediator to activate transcription. The beta-catenin transactivation domain binds directly to isolated Med12 and intact Mediator both in vitro and in vivo, and Mediator is recruited to Wnt-responsive genes in a beta-catenin-dependent manner.


Pssm-ID: 463471 [Multi-domain]  Cd Length: 210  Bit Score: 250.14  E-value: 1.68e-75
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1802 QMMHHPQSTLWGYNlVGQPQQPGFFLQNQSLTPGGSRLDPagSFVPtNTKQaLSNMLQRRSGAM-MQPPSLHAITSQQQL 1880
Cdd:pfam12144    1 QLMHHPQSALWGRN-MYQPQQMGFYTQNQPLPPGGPRLDP--PYRP-VRKP-LSKMLPTRPNYPgMMPTMMGSIMSLDQQ 75
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277088   1881 IQMKLLQQQQQQRLLRQA-----QTRPFQQGQPGDQaalfaaQARPSPQLPQYPGLQQaqtMPQGYTMYGTQMPLQQTsQ 1955
Cdd:pfam12144   76 LYKMKLYKQQPTMPQGQLlrqqlQARLLQQGMLGQQ------QARQMPPNPSYGSLQL---MPQGYTMYGSHMGLQQH-P 145
                          170       180       190       200       210       220
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 93277088   1956 QQAGSVVlSPSYNSRAYPAAH--SNPVLMERLRQIQQQPSGYVQQQASPYLQPLTGSQRLNHQALQ 2019
Cdd:pfam12144  146 SQAGGMV-PPSYSSQAYQGAHpgSNPALVDPLRQMQQRPSGYVHQQAPGYLHPLTNTQRFAHQPLQ 210
Med12 pfam09497
Transcription mediator complex subunit Med12; Med12 is a negative regulator of the ...
106-161 4.70e-17

Transcription mediator complex subunit Med12; Med12 is a negative regulator of the Gli3-dependent sonic hedgehog signalling pathway via its interaction with Gli3 within the RNA polymerase II transcriptional Mediator. A complex is formed between Med12, Med13, CDK8 and CycC which is responsible for suppression of transcription. This subunit forms part of the Kinase section of Mediator.


Pssm-ID: 462818  Cd Length: 63  Bit Score: 77.18  E-value: 4.70e-17
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 93277088    106 TARSQSAIHSWFSDLAG-NKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIK 161
Cdd:pfam09497    7 VTLTDTKREAWLRDLANpNVPLSKLSKKIPHGLKGKKLLEQLCSKNVPIPRAIWFIK 63
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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