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Conserved domains on  [gi|15599309|ref|NP_252803|]
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spermidine acetyltransferase [Pseudomonas aeruginosa PAO1]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 11418877)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  27367672|26818562|15581578|10940244
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-135 5.36e-11

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 56.20  E-value: 5.36e-11
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15599309  57 GFALYGPDADDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGFVEQGLAP 135
Cdd:COG0456   1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEV--REDNEAAIALYEKLGFEEVGERP 77
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-135 5.36e-11

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 56.20  E-value: 5.36e-11
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15599309  57 GFALYGPDADDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGFVEQGLAP 135
Cdd:COG0456   1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEV--REDNEAAIALYEKLGFEEVGERP 77
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 12-128 8.60e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 48.28  E-value: 8.60e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309    12 WETCIDLKVARHQAHFVASNLYSLAQSRFLPGFRAVGIHHGGRMVGFALYGP-DADDGHYWLYRLMIDRRYQGKGHGRAA 90
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIiDDEPPVGEIEGLAVAPEYRGKGIGTAL 80

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 15599309    91 LQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGF 128
Cdd:pfam00583  81 LQALLEWARERGCERIFLEV--AADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 47-96 9.09e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 9.09e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 15599309  47 VGIHHGGRMVGFALYGPDADDGHY-WLYRLMIDRRYQGKGHGRAALQRIIA 96
Cdd:cd04301   2 LVAEDDGEIVGFASLSPDGSGGDTaYIGDLAVLPEYRGKGIGSALLEAAEE 52
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 49-130 2.06e-03

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 36.83  E-value: 2.06e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   49 IHHGGRMVGFALYGPDADDGHywLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVAYAvdNDVARQLYRDAGF 128
Cdd:PRK09491  45 LTVNGQMAAFAITQVVLDEAT--LFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRAS--NAAAIALYESLGF 120


                 ..
gi 15599309  129 VE 130
Cdd:PRK09491 121 NE 122
 
Name Accession Description Interval E-value
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 57-135 5.36e-11

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 56.20  E-value: 5.36e-11
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15599309  57 GFALYGPDADDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGFVEQGLAP 135
Cdd:COG0456   1 GFALLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEV--REDNEAAIALYEKLGFEEVGERP 77
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-132 2.76e-08

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 50.38  E-value: 2.76e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   1 MIELRTITRDDWETCIDLkVARHQAHFVAS---------NLYSLAQSRFLPGFRAVGIHHGGRMVGFALYGP---DADDG 68
Cdd:COG1247   1 EMTIRPATPEDAPAIAAI-YNEAIAEGTATfeteppseeEREAWFAAILAPGRPVLVAEEDGEVVGFASLGPfrpRPAYR 79

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15599309  69 HYWLYRLMIDRRYQGKGHGRAALQRIIAsvRRTRRRSDRLMVAYAVDNDVARQLYRDAGFVEQG 132
Cdd:COG1247  80 GTAEESIYVDPDARGRGIGRALLEALIE--RARARGYRRLVAVVLADNEASIALYEKLGFEEVG 141
RimL COG1670
Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, ...
 1-135 3.33e-08

Protein N-acetyltransferase, RimJ/RimL family [Translation, ribosomal structure and biogenesis, Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441276 [Multi-domain]  Cd Length: 173  Bit Score: 50.38  E-value: 3.33e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   1 MIELRTITRDDWETCIDLKVARHQAHFVASNLYSLAQSRFL------------PGFRAVGIHHGGRMVGFA-LYGPDADD 67
Cdd:COG1670   7 RLRLRPLRPEDAEALAELLNDPEVARYLPGPPYSLEEARAWlerlladwadggALPFAIEDKEDGELIGVVgLYDIDRAN 86

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15599309  68 GHYWLyRLMIDRRYQGKGHGRAALQRIIAsVRRTRRRSDRLMVAYAVDNDVARQLYRDAGFVEQGLAP 135
Cdd:COG1670  87 RSAEI-GYWLAPAYWGKGYATEALRALLD-YAFEELGLHRVEAEVDPDNTASIRVLEKLGFRLEGTLR 152
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 12-128 8.60e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 48.28  E-value: 8.60e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309    12 WETCIDLKVARHQAHFVASNLYSLAQSRFLPGFRAVGIHHGGRMVGFALYGP-DADDGHYWLYRLMIDRRYQGKGHGRAA 90
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELVGFASLSIiDDEPPVGEIEGLAVAPEYRGKGIGTAL 80

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 15599309    91 LQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGF 128
Cdd:pfam00583  81 LQALLEWARERGCERIFLEV--AADNLAAIALYEKLGF 116
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 49-130 2.71e-07

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 45.91  E-value: 2.71e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309    49 IHHGGRMVGFALYGPDADDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVAYAvdndvARQLYRDAGF 128
Cdd:pfam13508   8 AEDDGKIVGFAALLPLDDEGALAELRLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNR-----AAAFYEKLGF 82


                  ..
gi 15599309   129 VE 130
Cdd:pfam13508  83 EE 84
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 2-141 2.97e-07

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 46.97  E-value: 2.97e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   2 IELRTITRDDwetcIDLKVARHQAHFVASNLYSLaqsrfLPGFRAVGIHHGGRMVGFALYGPDADDGHYWLyRLMIDRRY 81
Cdd:COG0454   1 MSIRKATPED----INFILLIEALDAELKAMEGS-----LAGAEFIAVDDKGEPIGFAGLRRLDDKVLELK-RLYVLPEY 70

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15599309  82 QGKGHGRAALQRIIASVRRTRRRSDRLMVAYavDNDVARQLYRDAGFVEQG--LAPWGETMA 141
Cdd:COG0454  71 RGKGIGKALLEALLEWARERGCTALELDTLD--GNPAAIRFYERLGFKEIEryVAYVGGEFE 130
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
4-132 2.21e-05

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 41.99  E-value: 2.21e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   4 LRTITRDDWETCIDLKVARHQAHFVASNLYSLAQSRFLPgfRAVGIHHGGRMVGFALYGP---DADDGHYWLYRLMIDRR 80
Cdd:COG3153   1 IRPATPEDAEAIAALLRAAFGPGREAELVDRLREDPAAG--LSLVAEDDGEIVGHVALSPvdiDGEGPALLLGPLAVDPE 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 15599309  81 YQGKGHGRAALQRIIASVRRTRRRSDRLMVayavdNDVARQLYRDAGFVEQG 132
Cdd:COG3153  79 YRGQGIGRALMRAALEAARERGARAVVLLG-----DPSLLPFYERFGFRPAG 125
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
54-132 4.12e-05

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 40.28  E-value: 4.12e-05
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15599309  54 RMVGFALYGPDaDDGHYWLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVayAVDNDVARQLYRDAGFVEQG 132
Cdd:COG3393   1 ELVAMAGVRAE-SPGVAEISGVYTHPEYRGRGLASALVAALAREALARGARTPFLYV--DADNPAARRLYERLGFRPVG 76
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 47-96 9.09e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.80  E-value: 9.09e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 15599309  47 VGIHHGGRMVGFALYGPDADDGHY-WLYRLMIDRRYQGKGHGRAALQRIIA 96
Cdd:cd04301   2 LVAEDDGEIVGFASLSPDGSGGDTaYIGDLAVLPEYRGKGIGSALLEAAEE 52
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 52-132 3.87e-04

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 38.41  E-value: 3.87e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309    52 GGRMVGFALYGpdaDDGHywLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMV---AYAVDndvarqLYRDAGF 128
Cdd:pfam13673  39 GGQIVGVIALR---DRGH--ISLLFVDPDYQGQGIGKALLEAVEDYAEKDGIKLSELTVnasPYAVP------FYEKLGF 107


                  ....
gi 15599309   129 VEQG 132
Cdd:pfam13673 108 RATG 111
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 2-130 1.47e-03

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 36.89  E-value: 1.47e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   2 IELRTITRDDWETCIDLkvarhqahfVASNLYSLAQSRFLpgfravGIHHGGRMVGFALYGPDaDDGHYWLYRLMIDRRY 81
Cdd:COG1246   1 MTIRPATPDDVPAILEL---------IRPYALEEEIGEFW------VAEEDGEIVGCAALHPL-DEDLAELRSLAVHPDY 64

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*....
gi 15599309  82 QGKGHGRAALQRIIAsvrrtRRRSDRLMVAYAVDNDVARQLYRDAGFVE 130
Cdd:COG1246  65 RGRGIGRRLLEALLA-----EARELGLKRLFLLTTSAAIHFYEKLGFEE 108
rimI PRK09491
ribosomal-protein-alanine N-acetyltransferase; Provisional
 49-130 2.06e-03

ribosomal-protein-alanine N-acetyltransferase; Provisional


Pssm-ID: 181904 [Multi-domain]  Cd Length: 146  Bit Score: 36.83  E-value: 2.06e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599309   49 IHHGGRMVGFALYGPDADDGHywLYRLMIDRRYQGKGHGRAALQRIIASVRRTRRRSDRLMVAYAvdNDVARQLYRDAGF 128
Cdd:PRK09491  45 LTVNGQMAAFAITQVVLDEAT--LFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWLEVRAS--NAAAIALYESLGF 120


                 ..
gi 15599309  129 VE 130
Cdd:PRK09491 121 NE 122
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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