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Conserved domains on  [gi|15599007|ref|NP_252501|]
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iron-binding protein IscA [Pseudomonas aeruginosa PAO1]

Protein Classification

iron-sulfur cluster assembly accessory protein( domain architecture ID 1258)

iron-sulfur cluster assembly accessory protein from the HesB/IscA family is involved in the assembly of iron-sulfur proteins

CATH:  2.60.300.12
Gene Ontology:  GO:0005506|GO:0016226
SCOP:  4003950

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Fe-S_biosyn super family cl00400
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 1-107 1.13e-57

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


The actual alignment was detected with superfamily member PRK09502:

Pssm-ID: 444888 [Multi-domain]  Cd Length: 107  Bit Score: 173.13  E-value: 1.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    1 MAISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFT 80
Cdd:PRK09502   1 MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFV 80

                         90       100
                 ....*....|....*....|....*..
gi 15599007   81 REGLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:PRK09502  81 KEGLNEGFKFTNPNVKDECGCGESFHV 107
 
Name Accession Description Interval E-value
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
1-107 1.13e-57

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 173.13  E-value: 1.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    1 MAISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFT 80
Cdd:PRK09502   1 MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFV 80

                         90       100
                 ....*....|....*....|....*..
gi 15599007   81 REGLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:PRK09502  81 KEGLNEGFKFTNPNVKDECGCGESFHV 107
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 2-107 2.16e-57

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 172.26  E-value: 2.16e-57
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007   2 AISMTEAAAKHVQRSLEGRG-KGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFT 80
Cdd:COG0316   1 PITLTDAAAKRIKRLLAKEGnPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*..
gi 15599007  81 REGLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 3-107 8.84e-57

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 170.78  E-value: 8.84e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     3 ISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFTRE 82
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFGLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVKE 80

                          90       100
                  ....*....|....*....|....*
gi 15599007    83 GLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:TIGR02011  81 GLNEGFKFTNPNVKDECGCGESFHV 105
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 1-103 4.10e-25

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 90.78  E-value: 4.10e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     1 MAISMTEAAAKHVQRSLEGrgKGEGIRLGVRTTG-------CS-GLAYVLEFVDELAAE-DLVFESHGVKVIIDPKSLVY 71
Cdd:pfam01521   1 MKITITDAAAERLKKLLAG--DKKELRLDVDDGGgpyskggCSiGGKFSLVLVDEPDPDyDEVIESNGGPIYVDSYSLPF 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 15599007    72 LD-GTELDFTREGLNEGFKFNNPNVRGECGCGE 103
Cdd:pfam01521  79 LDeGLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
 
Name Accession Description Interval E-value
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
1-107 1.13e-57

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 173.13  E-value: 1.13e-57
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    1 MAISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFT 80
Cdd:PRK09502   1 MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFV 80

                         90       100
                 ....*....|....*....|....*..
gi 15599007   81 REGLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:PRK09502  81 KEGLNEGFKFTNPNVKDECGCGESFHV 107
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 2-107 2.16e-57

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 172.26  E-value: 2.16e-57
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007   2 AISMTEAAAKHVQRSLEGRG-KGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFT 80
Cdd:COG0316   1 PITLTDAAAKRIKRLLAKEGnPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*..
gi 15599007  81 REGLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 3-107 8.84e-57

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 170.78  E-value: 8.84e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     3 ISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFTRE 82
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFGLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVKE 80

                          90       100
                  ....*....|....*....|....*
gi 15599007    83 GLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:TIGR02011  81 GLNEGFKFTNPNVKDECGCGESFHV 105
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 6-107 5.05e-46

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 143.49  E-value: 5.05e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     6 TEAAAKHVQRSLEGRGK-GEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFTREGL 84
Cdd:TIGR00049   3 TDSAAKRIKALLAGEGEpNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEELL 82

                          90       100
                  ....*....|....*....|...
gi 15599007    85 NEGFKFNNPNVRGECGCGESFNV 107
Cdd:TIGR00049  83 GSGFTFTNPNAKGTCGCGKSFSV 105
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 3-107 6.98e-42

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 133.03  E-value: 6.98e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     3 ISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFTRE 82
Cdd:TIGR01997   3 ITLTDAAAIHIRELVAKRPEAVGIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDFVRT 82

                          90       100
                  ....*....|....*....|....*
gi 15599007    83 GLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:TIGR01997  83 TLRQGFKFNNPNATSACGCGESFEL 107
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 3-107 8.78e-41

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 131.01  E-value: 8.78e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    3 ISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDFTRE 82
Cdd:PRK09504  18 LTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVRE 97

                         90       100
                 ....*....|....*....|....*
gi 15599007   83 GLNEGFKFNNPNVRGECGCGESFNV 107
Cdd:PRK09504  98 GLNQIFKFHNPKAQNECGCGESFGV 122
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 1-103 4.10e-25

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 90.78  E-value: 4.10e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007     1 MAISMTEAAAKHVQRSLEGrgKGEGIRLGVRTTG-------CS-GLAYVLEFVDELAAE-DLVFESHGVKVIIDPKSLVY 71
Cdd:pfam01521   1 MKITITDAAAERLKKLLAG--DKKELRLDVDDGGgpyskggCSiGGKFSLVLVDEPDPDyDEVIESNGGPIYVDSYSLPF 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 15599007    72 LD-GTELDFTREGLNEGFKFNNPNVRGECGCGE 103
Cdd:pfam01521  79 LDeGLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 1-107 2.40e-24

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 88.83  E-value: 2.40e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    1 MAISMTEAAAKHVQRSLEGRGKGE-GIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDF 79
Cdd:PRK13623   8 LPLVFTDAAAAKVKELIEEEGNPDlKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDY 87

                         90       100       110
                 ....*....|....*....|....*....|
gi 15599007   80 TrEGLnEGFKF--NNPNVRGECGCGESFNV 107
Cdd:PRK13623  88 T-EGL-EGSRFviKNPNAKTTCGCGSSFSI 115
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 2-107 4.01e-18

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 74.58  E-value: 4.01e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    2 AISMTEAAAKHVQ--RSLEGRGKGEGIRLGVRTTGCSGLAYVLEFVDELAAEDLVFESHGVKVIIDPKSLVYLDGTELDF 79
Cdd:PLN03082  54 AVHMTDNCIRRLKelQTSEPSAEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVDY 133

                         90       100
                 ....*....|....*....|....*....
gi 15599007   80 TREGLNEGFKFN-NPNVRGECGCGESFNV 107
Cdd:PLN03082 134 VEELIRSAFVVStNPSAVGGCSCKSSFMV 162
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
3-94 1.04e-04

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 39.23  E-value: 1.04e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599007    3 ISMTEAAAKHVQRSLEGRGKGEGIRLGVRTTGCS----GLAYVLEfvDELAAEDLVFESHGVKVIIDPKSLVYLDGTELD 78
Cdd:PRK11190   2 ITISDAAQAHFAKLLANQEEGTQIRVFVINPGTPnaecGVSYCPP--DAVEATDTELKFDGFSAYVDELSAPFLEDAEID 79

                         90
                 ....*....|....*.
gi 15599007   79 FTREGLNEGFKFNNPN 94
Cdd:PRK11190  80 FVTDQLGSQLTLKAPN 95
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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