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Conserved domains on  [gi|15598296|ref|NP_251790|]
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type II secretion system protein H [Pseudomonas aeruginosa PAO1]

Protein Classification

type II secretion system protein( domain architecture ID 11450365)

type II secretion system protein similar to Pseudomonas aeruginosa type II secretion system protein H, which is involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins; required for the translocation of a variety of enzymes across the outer membrane; pilin similar to fimbrial protein PilE, which is required for pilus biogenesis, as well as fimbrial protein PilA, which is responsible for the adhesive property of the pilus

Gene Ontology:  GO:0005886|GO:0015628|GO:0015627
PubMed:  30767847|1365398

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-67 2.02e-13

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


:

Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 61.79  E-value: 2.02e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLR 67
Cdd:COG4970   5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTLS 71
typeII_sec_gspH super family cl46935
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 2-149 2.02e-11

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


The actual alignment was detected with superfamily member TIGR01708:

Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 58.73  E-value: 2.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15598296     2 RASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLERdayQVLRYDEA 81
Cdd:TIGR01708   1 HRQSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGS---RGIRFGER 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15598296    82 KARWLPVarDSHRLPEWAeltfeldGQPLVLAgskgekeqKKGTDQPqLLILSSGELSPFRLRLAERG 149
Cdd:TIGR01708  78 ASLALPV--DEVKMTDWT-------GQETVVA--------DRQTVLT-VLFLPDGRASPMDMTLQRKG 127
 
Name Accession Description Interval E-value
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-67 2.02e-13

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 61.79  E-value: 2.02e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLR 67
Cdd:COG4970   5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTLS 71
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 2-149 2.02e-11

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 58.73  E-value: 2.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15598296     2 RASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLERdayQVLRYDEA 81
Cdd:TIGR01708   1 HRQSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGS---RGIRFGER 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15598296    82 KARWLPVarDSHRLPEWAeltfeldGQPLVLAgskgekeqKKGTDQPqLLILSSGELSPFRLRLAERG 149
Cdd:TIGR01708  78 ASLALPV--DEVKMTDWT-------GQETVVA--------DRQTVLT-VLFLPDGRASPMDMTLQRKG 127
TIGR02596 TIGR02596
Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in ...
 7-70 7.79e-10

Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in Verrucomicrobium spinosum and a somewhat smaller paralogous family in Chthoniobacter flavus. All members share a type IV pilin-like N-terminal leader sequence (TIGR02532). These proteins occur in the four-gene Verru_Chthon cassette, in which two other genes likewise encode a cleavage/methylation domain. Most of these cassettes occur next to an unusually large PEP-CTERM protein with an autotransporter domain. [Cell envelope, Surface structures]


Pssm-ID: 274219 [Multi-domain]  Cd Length: 195  Bit Score: 55.17  E-value: 7.79e-10
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15598296     7 FTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLER 70
Cdd:TIGR02596   1 FTLVELLVVIAIAALLMALSTPVVNQVLAAQQLGSSATRLANELAAAAQLAVRENRPVEVRFLR 64
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 4.95e-08

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 46.60  E-value: 4.95e-08
                          10        20
                  ....*....|....*....|....*..
gi 15598296     1 MRASRGFTLIELMVVMVIISVLIGLAV 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
PRK10574 PRK10574
putative major pilin subunit; Provisional
 1-25 1.28e-06

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 45.79  E-value: 1.28e-06
                         10        20
                 ....*....|....*....|....*..
gi 15598296    1 MRASRGFTLIELMVVMVIISVL--IGL 25
Cdd:PRK10574   1 MDKQRGFTLIELMVVIAIIAILsaIGI 27
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 6-27 1.67e-06

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 44.04  E-value: 1.67e-06
                         10        20
                 ....*....|....*....|..
gi 15598296    6 GFTLIELMVVMVIISVLIGLAV 27
Cdd:NF040999   3 GFTLIEMLIVLLIISVLLLLFV 24
 
Name Accession Description Interval E-value
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-67 2.02e-13

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 61.79  E-value: 2.02e-13
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLR 67
Cdd:COG4970   5 RRRQRGFTLIELLVVLAILAILAAIAVPSFSSLIARQRLRAAANELAAALRLARSEAIRRGQPVTLS 71
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 1-64 1.08e-11

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 58.39  E-value: 1.08e-11
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGfASTSRELDSEAERLAGLIGVLTDEAVLDNREY 64
Cdd:COG2165   7 RRRQRGFTLIELLVVIAIIGILAALALPALQ-GARERARRAELRSNLRQIQQALERYRLDNGRY 69
typeII_sec_gspH TIGR01708
type II secretion system protein H; This model represents GspH, protein H of the main terminal ...
 2-149 2.02e-11

type II secretion system protein H; This model represents GspH, protein H of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130769 [Multi-domain]  Cd Length: 143  Bit Score: 58.73  E-value: 2.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15598296     2 RASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLERdayQVLRYDEA 81
Cdd:TIGR01708   1 HRQSGFTLIELLVVLAIMGLVAAAAALSLVSHYGTKSLDQVAGRLAARLRLAQTSARATGRPVLLRLGS---RGIRFGER 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15598296    82 KARWLPVarDSHRLPEWAeltfeldGQPLVLAgskgekeqKKGTDQPqLLILSSGELSPFRLRLAERG 149
Cdd:TIGR01708  78 ASLALPV--DEVKMTDWT-------GQETVVA--------DRQTVLT-VLFLPDGRASPMDMTLQRKG 127
TIGR02596 TIGR02596
Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in ...
 7-70 7.79e-10

Verru_Chthon cassette protein D; This model describes a nearly twenty member protein family in Verrucomicrobium spinosum and a somewhat smaller paralogous family in Chthoniobacter flavus. All members share a type IV pilin-like N-terminal leader sequence (TIGR02532). These proteins occur in the four-gene Verru_Chthon cassette, in which two other genes likewise encode a cleavage/methylation domain. Most of these cassettes occur next to an unusually large PEP-CTERM protein with an autotransporter domain. [Cell envelope, Surface structures]


Pssm-ID: 274219 [Multi-domain]  Cd Length: 195  Bit Score: 55.17  E-value: 7.79e-10
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15598296     7 FTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLER 70
Cdd:TIGR02596   1 FTLVELLVVIAIAALLMALSTPVVNQVLAAQQLGSSATRLANELAAAAQLAVRENRPVEVRFLR 64
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
1-27 8.45e-09

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 51.23  E-value: 8.45e-09
                        10        20
                ....*....|....*....|....*..
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAV 27
Cdd:COG4968   6 RRRQRGFTLIELMIVVAIIGILAAIAI 32
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
5-27 2.98e-08

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 49.15  E-value: 2.98e-08
                        10        20
                ....*....|....*....|...
gi 15598296   5 RGFTLIELMVVMVIISVLIGLAV 27
Cdd:COG4537  12 KGFTLIEMLIVLLIISILLLIAV 34
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 1-27 4.95e-08

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 46.60  E-value: 4.95e-08
                          10        20
                  ....*....|....*....|....*..
gi 15598296     1 MRASRGFTLIELMVVMVIISVLIGLAV 27
Cdd:pfam07963   1 MRKQRGFTLIELLVALAILAILLAAAL 27
PRK10574 PRK10574
putative major pilin subunit; Provisional
 1-25 1.28e-06

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 45.79  E-value: 1.28e-06
                         10        20
                 ....*....|....*....|....*..
gi 15598296    1 MRASRGFTLIELMVVMVIISVL--IGL 25
Cdd:PRK10574   1 MDKQRGFTLIELMVVIAIIAILsaIGI 27
pilin_ComGC NF040999
competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major ...
 6-27 1.67e-06

competence type IV pilus major pilin ComGC; ComGC, encoded in the comG operon, is the major pilin of a type IV pilus involved in natural transformation of monoderm bacteria (those lacking an outer membrane) such as Bacillus subtilis and Streptococcus pneumoniae. In the seed alignment, Bacillus proteins have a pair of Cys residues likely to form a disulfide bond while Streptococcus proteins lack Cys residues.


Pssm-ID: 468929  Cd Length: 84  Bit Score: 44.04  E-value: 1.67e-06
                         10        20
                 ....*....|....*....|..
gi 15598296    6 GFTLIELMVVMVIISVLIGLAV 27
Cdd:NF040999   3 GFTLIEMLIVLLIISVLLLLFV 24
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
1-44 1.87e-06

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 45.56  E-value: 1.87e-06
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAV-LSTGFASTSRELDSEAE 44
Cdd:COG4966   1 RRRQRGFTLVELMVALAIGLIVLAAVLqLFLSSRRSYRTQEALAR 45
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
1-27 2.90e-06

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 44.31  E-value: 2.90e-06
                        10        20
                ....*....|....*....|....*...
gi 15598296   1 MRAS-RGFTLIELMVVMVIISVLIGLAV 27
Cdd:COG4969   1 MKKKqKGFTLIELMIVVAIIGILAAIAI 28
PRK10506 PRK10506
prepilin peptidase-dependent protein;
1-71 5.11e-06

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 44.21  E-value: 5.11e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15598296    1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRELDSEAERLAGLIGVLTDEAVLDNREYGLRLERD 71
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSAWGLYGWQRWQQRQRLWQTAQQLLDFLLRLQEDANWHNRDHILWLIRE 75
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-47 5.81e-06

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 42.66  E-value: 5.81e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 15598296   1 MRASRGFTLIELMVVMVIISV----LIGLAVLSTGFASTSRELdSEAERLA 47
Cdd:COG4967   7 RRRQRGFTLIEVLVALVILSIgllgLAGLQAASLRSSQDARQR-TQAALLA 56
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 5-27 6.77e-06

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 40.75  E-value: 6.77e-06
                          10        20
                  ....*....|....*....|...
gi 15598296     5 RGFTLIELMVVMVIISVLIGLAV 27
Cdd:TIGR02532   2 RGFTLIELLVVLAILGILALIAL 24
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 1-49 2.82e-05

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 41.54  E-value: 2.82e-05
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|
gi 15598296   1 MRASRGFTLIELMVVMVIISVLIGLAVLSTGFASTSRE-LDSEAERLAGL 49
Cdd:COG4795   5 RRRQRGFTLLELLVALAIFALLLLAAYRGLDSVLRSRErLEQQAERLQEL 54
typeII_sec_gspG TIGR01710
type II secretion system protein G; This model represents GspG, protein G of the main terminal ...
 5-64 7.07e-05

type II secretion system protein G; This model represents GspG, protein G of the main terminal branch of the general secretion pathway, also called type II secretion. It transports folded proteins across the bacterial outer membrane and is widely distributed in Gram-negative pathogens. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]


Pssm-ID: 130771 [Multi-domain]  Cd Length: 134  Bit Score: 40.49  E-value: 7.07e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15598296     5 RGFTLIELMVVMVIISVLIGLAVLSTgfasTSRELDSEAER----LAGLIGVLtDEAVLDNREY 64
Cdd:TIGR01710   1 RGFTLLEIMVVLVILGLLAALVAPKL----FSQADKAKAQVakaqIKALKNAL-DMYRLDNGRY 59
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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